DNASU Plasmid | DDX28 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: DDX28

Gene Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 28

Original Clone ID:

ccsbBroadEn_08588

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC024273.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00515881

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1621nts

Open reading frame: 1 to 1621

Reference Sequence Alignment

ATGGCTCTAGCGCGGCCGGTGCGGCTCTTTTCCCTCGTGACTCGGTTGCTCCTGGCGCCG
CGACGGGGCCTCACGGTCCGCAGTCCCGACGAACCCCTGCCGGTGGTGCGCATCCCAGTG
GCTCTACAGCGGCAGTTGGAACAGCGGCAGAGCAGGCGGCGGAACCTCCCGAGGCCGGTG
CTGGTTCGACCCGGACCGCTGCTGGTTTCGGCGCGGCGGCCGGAGTTGAACCAGCCGGCG
CGCCTCACACTGGGCCGTTGGGAGCGCGCGCCGCTAGCCTCTCAAGGCTGGAAGAGTCGA
CGCGCGCGTCGGGACCACTTCTCCATCGAGCGCGCGCAACAGGAGGCGCCAGCGGTGCGA
AAGCTCTCGTCTAAGGGCAGCTTTGCTGACCTGGGCCTGGAGCCCCGTGTGCTGCACGCA
CTACAGGAGGCTGCGCCTGAAGTCGTTCAGCCCACAACCGTGCAGTCTAGCACCATCCCC
TCACTACTTCGCGGCCGCCATGTCGTTTGCGCCGCAGAAACCGGCAGTGGCAAGACTCTC
AGCTACCTCCTGCCGCTGCTTCAACGGCTCTTGGGCCAGCCAAGCCTGGACTCCCTTCCT
ATCCCCGCGCCCCGAGGCCTGGTCCTTGTTCCTTCCCGAGAATTGGCCCAACAGGTGCGG
GCTGTGGCCCAACCCTTGGGCCGCTCCTTGGGCCTGCTGGTGCGGGACCTGGAGGGAGGC
CACGGCATGCGTAGGATCAGGCTGCAGCTGTCCAGACAGCCTTCAGCAGATGTGCTTGTG
GCCACTCCAGGGGCTCTGTGGAAGGCCCTGAAAAGTCGACTGATCAGTCTGGAGCAACTC
TCCTTCTTGGTGTTGGATGAGGCAGACACACTGCTGGATGAAAGCTTCCTGGAACTGGTG
GACTACATCTTAGAGAAGAGCCACATAGCAGAAGGCCCAGCTGACTTGGAAGACCCCTTC
AATCCCAAAGCTCAGTTAGTGCTGGTAGGAGCCACATTTCCCGAAGGTGTAGGCCAGTTG
CTGAATAAAGTCGCCAGCCCAGATGCTGTCACCACCATCACCAGCTCCAAGCTCCACTGT
ATCATGCCTCATGTGAAACAGACATTTCTGAGACTGAAGGGAGCAGATAAGGTGGCCGAG
CTGGTGCACATCCTCAAGCATCGTGACAGAGCAGAAAGGACTGGTCCCTCAGGAACTGTT
CTGGTGTTCTGTAATAGCTCCAGCACTGTGAACTGGCTGGGATATATTCTGGATGACCAC
AAAATCCAACACCTAAGGTTGCAGGGGCAAATGCCAGCCTTGATGAGGGTAGGAATCTTC
CAGTCCTTCCAGAAGAGCTCCCGAGACATACTTCTCTGCACAGACATAGCCTCTCGGGGC
CTGGACAGCACTGGTGTGGAGCTGGTTGTCAATTATGATTTCCCCCCAACGCTGCAAGAT
TACATCCACAGAGCAGGGAGAGTGGGCCGTGTGGGGAGCGAGGTGCCAGGCACCGTCATC
AGTTTTGTGACCCATCCCTGGGATGTGAGCCTGGTTCAGAAGATTGAGCTGGCGGCTCGC
CGAAGGAGAAGTCTTCCAGGACTAGCATCCTCGGTGAAAGAGCCTTTGCCCCAAGCAACC

Protein sequence predicted based on nucleotide sequence

MALARPVRLFSLVTRLLLAPRRGLTVRSPDEPLPVVRIPVALQRQLEQRQSRRRNLPRPV
LVRPGPLLVSARRPELNQPARLTLGRWERAPLASQGWKSRRARRDHFSIERAQQEAPAVR
KLSSKGSFADLGLEPRVLHALQEAAPEVVQPTTVQSSTIPSLLRGRHVVCAAETGSGKTL
SYLLPLLQRLLGQPSLDSLPIPAPRGLVLVPSRELAQQVRAVAQPLGRSLGLLVRDLEGG
HGMRRIRLQLSRQPSADVLVATPGALWKALKSRLISLEQLSFLVLDEADTLLDESFLELV
DYILEKSHIAEGPADLEDPFNPKAQLVLVGATFPEGVGQLLNKVASPDAVTTITSSKLHC
IMPHVKQTFLRLKGADKVAELVHILKHRDRAERTGPSGTVLVFCNSSSTVNWLGYILDDH
KIQHLRLQGQMPALMRVGIFQSFQKSSRDILLCTDIASRGLDSTGVELVVNYDFPPTLQD
YIHRAGRVGRVGSEVPGTVISFVTHPWDVSLVQKIELAARRRRSLPGLASSVKEPLPQAT

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

DDX28

Symbol Nomenclature :

DDX28

SYNONYM :

MDDX28

Designation :

DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 28|mitochondrial DEAD box protein 28|mitochondrial DEAD-box polypeptide 28|probable ATP-dependent RNA helicase DDX28

Full Nomenclature :

DEAD (Asp-Glu-Ala-Asp) box polypeptide 28

GENEID :

55794

HGNC :

17330

MIM :

607618

Vega :

OTTHUMG00000137549

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00515881      ATGGCTCTAGCGCGGCCGGTGCGGCTCTTTTCCCTCGTGACTCGGTTGCTCCTGGCGCCG
NM_018380.3       ATGGCTCTAACGCGGCCGGTGCGGCTCTTTTCCCTCGTGACTCGGTTGCTCCTGGCGCCG
                  *********.**************************************************

HsCD00515881      CGACGGGGCCTCACGGTCCGCAGTCCCGACGAACCCCTGCCGGTGGTGCGCATCCCAGTG
NM_018380.3       CGACGGGGCCTCACGGTCCGCAGTCCCGACGAACCCCTGCCGGTGGTGCGCATCCCAGTG
                  ************************************************************

HsCD00515881      GCTCTACAGCGGCAGTTGGAACAGCGGCAGAGCAGGCGGCGGAACCTCCCGAGGCCGGTG
NM_018380.3       GCTCTACAGCGGCAGTTGGAACAGCGGCAGAGCAGGCGGCGGAACCTCCCGAGGCCGGTG
                  ************************************************************

HsCD00515881      CTGGTTCGACCCGGACCGCTGCTGGTTTCGGCGCGGCGGCCGGAGTTGAACCAGCCGGCG
NM_018380.3       CTGGTTCGACCCGGACCGCTGCTGGTTTCGGCGCGGCGGCCGGAGTTGAACCAGCCGGCG
                  ************************************************************

HsCD00515881      CGCCTCACACTGGGCCGTTGGGAGCGCGCGCCGCTAGCCTCTCAAGGCTGGAAGAGTCGA
NM_018380.3       CGCCTCACACTGGGCCGTTGGGAGCGCGCGCCGCTAGCCTCTCAAGGCTGGAAGAGTCGA
                  ************************************************************

HsCD00515881      CGCGCGCGTCGGGACCACTTCTCCATCGAGCGCGCGCAACAGGAGGCGCCAGCGGTGCGA
NM_018380.3       CGCGCGCGTCGGGACCACTTCTCCATCGAGCGCGCGCAACAGGAGGCGCCAGCGGTGCGA
                  ************************************************************

HsCD00515881      AAGCTCTCGTCTAAGGGCAGCTTTGCTGACCTGGGCCTGGAGCCCCGTGTGCTGCACGCA
NM_018380.3       AAGCTCTCGTCTAAGGGCAGCTTTGCTGACCTGGGCCTGGAGCCCCGTGTGCTGCACGCA
                  ************************************************************

HsCD00515881      CTACAGGAGGCTGCGCCTGAAGTCGTTCAGCCCACAACCGTGCAGTCTAGCACCATCCCC
NM_018380.3       CTACAGGAGGCTGCGCCTGAAGTCGTTCAGCCCACAACCGTGCAGTCTAGCACCATCCCC
                  ************************************************************

HsCD00515881      TCACTACTTCGCGGCCGCCATGTCGTTTGCGCCGCAGAAACCGGCAGTGGCAAGACTCTC
NM_018380.3       TCACTACTTCGCGGCCGCCATGTCGTTTGCGCCGCAGAAACCGGCAGTGGCAAGACTCTC
                  ************************************************************

HsCD00515881      AGCTACCTCCTGCCGCTGCTTCAACGGCTCTTGGGCCAGCCAAGCCTGGACTCCCTTCCT
NM_018380.3       AGCTACCTCCTGCCGCTGCTTCAACGGCTCTTGGGCCAGCCAAGCCTGGACTCCCTTCCT
                  ************************************************************

HsCD00515881      ATCCCCGCGCCCCGAGGCCTGGTCCTTGTTCCTTCCCGAGAATTGGCCCAACAGGTGCGG
NM_018380.3       ATCCCCGCGCCCCGAGGCCTGGTCCTTGTTCCTTCCCGAGAATTGGCCCAACAGGTGCGG
                  ************************************************************

HsCD00515881      GCTGTGGCCCAACCCTTGGGCCGCTCCTTGGGCCTGCTGGTGCGGGACCTGGAGGGAGGC
NM_018380.3       GCTGTGGCCCAACCCTTGGGCCGCTCCTTGGGCCTGCTGGTGCGGGACCTGGAGGGAGGC
                  ************************************************************

HsCD00515881      CACGGCATGCGTAGGATCAGGCTGCAGCTGTCCAGACAGCCTTCAGCAGATGTGCTTGTG
NM_018380.3       CACGGCATGCGTAGGATCAGGCTGCAGCTGTCCAGACAGCCTTCAGCAGATGTGCTTGTG
                  ************************************************************

HsCD00515881      GCCACTCCAGGGGCTCTGTGGAAGGCCCTGAAAAGTCGACTGATCAGTCTGGAGCAACTC
NM_018380.3       GCCACTCCAGGGGCTCTGTGGAAGGCCCTGAAAAGTCGACTGATCAGTCTGGAGCAACTC
                  ************************************************************

HsCD00515881      TCCTTCTTGGTGTTGGATGAGGCAGACACACTGCTGGATGAAAGCTTCCTGGAACTGGTG
NM_018380.3       TCCTTCTTGGTGTTGGATGAGGCAGACACACTGCTGGATGAAAGCTTCCTGGAACTGGTG
                  ************************************************************

HsCD00515881      GACTACATCTTAGAGAAGAGCCACATAGCAGAAGGCCCAGCTGACTTGGAAGACCCCTTC
NM_018380.3       GACTACATCTTAGAGAAGAGCCACATAGCAGAAGGCCCAGCTGACTTGGAAGACCCCTTC
                  ************************************************************

HsCD00515881      AATCCCAAAGCTCAGTTAGTGCTGGTAGGAGCCACATTTCCCGAAGGTGTAGGCCAGTTG
NM_018380.3       AATCCCAAAGCTCAGTTAGTGCTGGTAGGAGCCACATTTCCCGAAGGTGTAGGCCAGTTG
                  ************************************************************

HsCD00515881      CTGAATAAAGTCGCCAGCCCAGATGCTGTCACCACCATCACCAGCTCCAAGCTCCACTGT
NM_018380.3       CTGAATAAAGTCGCCAGCCCAGATGCTGTCACCACCATCACCAGCTCCAAGCTCCACTGT
                  ************************************************************

HsCD00515881      ATCATGCCTCATGTGAAACAGACATTTCTGAGACTGAAGGGAGCAGATAAGGTGGCCGAG
NM_018380.3       ATCATGCCTCATGTGAAACAGACATTTCTGAGACTGAAGGGAGCAGATAAGGTGGCCGAG
                  ************************************************************

HsCD00515881      CTGGTGCACATCCTCAAGCATCGTGACAGAGCAGAAAGGACTGGTCCCTCAGGAACTGTT
NM_018380.3       CTGGTGCACATCCTCAAGCATCGTGACAGAGCAGAAAGGACTGGTCCCTCAGGAACTGTT
                  ************************************************************

HsCD00515881      CTGGTGTTCTGTAATAGCTCCAGCACTGTGAACTGGCTGGGATATATTCTGGATGACCAC
NM_018380.3       CTGGTGTTCTGTAATAGCTCCAGCACTGTGAACTGGCTGGGATATATTCTGGATGACCAC
                  ************************************************************

HsCD00515881      AAAATCCAACACCTAAGGTTGCAGGGGCAAATGCCAGCCTTGATGAGGGTAGGAATCTTC
NM_018380.3       AAAATCCAACACCTAAGGTTGCAGGGGCAAATGCCAGCCTTGATGAGGGTAGGAATCTTC
                  ************************************************************

HsCD00515881      CAGTCCTTCCAGAAGAGCTCCCGAGACATACTTCTCTGCACAGACATAGCCTCTCGGGGC
NM_018380.3       CAGTCCTTCCAGAAGAGCTCCCGAGACATACTTCTCTGCACAGACATAGCCTCTCGGGGC
                  ************************************************************

HsCD00515881      CTGGACAGCACTGGTGTGGAGCTGGTTGTCAATTATGATTTCCCCCCAACGCTGCAAGAT
NM_018380.3       CTGGACAGCACTGGTGTGGAGCTGGTTGTCAATTATGATTTCCCCCCAACGCTGCAAGAT
                  ************************************************************

HsCD00515881      TACATCCACAGAGCAGGGAGAGTGGGCCGTGTGGGGAGCGAGGTGCCAGGCACCGTCATC
NM_018380.3       TACATCCACAGAGCAGGGAGAGTGGGCCGTGTGGGGAGCGAGGTGCCAGGCACCGTCATC
                  ************************************************************

HsCD00515881      AGTTTTGTGACCCATCCCTGGGATGTGAGCCTGGTTCAGAAGATTGAGCTGGCGGCTCGC
NM_018380.3       AGTTTTGTGACCCATCCCTGGGATGTGAGCCTGGTTCAGAAGATTGAGCTGGCGGCTCGC
                  ************************************************************

HsCD00515881      CGAAGGAGAAGTCTTCCAGGACTAGCATCCTCGGTGAAAGAGCCTTTGCCCCAAGCAACC
NM_018380.3       CGAAGGAGAAGTCTTCCAGGACTAGCATCCTCGGTGAAAGAGCCTTTGCCCCAAGCAACC
                  ************************************************************

HsCD00515881      ---
NM_018380.3       TGA