DNASU Plasmid | FOXN4 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: FOXN4

Gene Name: forkhead box N4

Original Clone ID:

ccsbBroadEn_09470

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC146825.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00515888

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1552nts

Open reading frame: 1 to 1552

Reference Sequence Alignment

ATGATAGAAAGTGACACCTCATCCATAATGTCAGGAATTATTCGAAACTCAGGGCAAAAT
CACCACCCCTCTCGACAGGAATACAGGCTTCTAGCCACCACCAGCGATGATGACCTTCCC
GGGGACCTGCAGTCGCTGTCGTGGCTCACGGCGGTGGATGTGCCTCGGCTGCAGCAGATG
GCAAGTGGCCGCGTGGACCTGGGTGGCCCCTGCGTGCCACATCCACACCCAGGTGCCTTG
GCTGGGGTGGCCGACCTGCATGTGGGAGCCACTCCAAGTCCCCTTCTCCATGGCCCAGCA
GGCATGGCCCCCCGAGGCATGCCAGGTCTGGGCCCCATAACTGGCCACAGAGACAGCATG
AGCCAGTTCCCCGTGGGGGGCCAGCCCTCATCTGGGCTGCAGGACCCGCCGCATCTGTAC
TCACCTGCCACCCAACCACAGTTCCCGCTCCCCCCGGGTGCCCAGCAGTGCCCTCCTGTG
GGCCTCTATGGCCCCCCATTTGGGGTGCGGCCCCCCTACCCCCAGCCCCACGTGGCTGTG
CATTCATCTCAAGAACTGCACCCCAAACACTACCCCAAGCCCATCTACTCGTACAGCTGT
CTGATCGCCATGGCCCTGAAGAACAGCAAGACAGGCAGCCTGCCTGTGAGCGAGATCTAC
AGCTTCATGAAGGAGCACTTCCCCTACTTCAAGACGGCCCCCGACGGGTGGAAGAACTCG
GTGCGGCACAACCTGTCTCTGAACAAGTGCTTCGAGAAGGTGGAGAACAAGATGAGCGGC
TCCTCCCGCAAGGGCTGCCTGTGGGCTCTGAACCTGGCCCGCATCGACAAGATGGAGGAG
GAGATGCACAAGTGGAAGAGGAAGGACCTGGCTGCCATCCACCGGAGTATGGCCAACCCT
GAGGAGTTGGACAAGCTGATCTCCGACCGGCCTGAAAGCTGCCGGCGCCCCGGCAAACCG
GGGGAACCAGAGGCCCCCGTGCTGACTCACGCCACCACAGTGGCCGTGGCGCATGGCTGC
CTGGCTGTCTCCCAGCTCCCACCCCAGCCACTGATGACCCTGTCCCTGCAGTCAGTCCCC
CTGCACCACCAGGTCCAGCCCCAGGCACATCTTGCTACAGACTCTCCAGCACCAGCCCAG
ACCCCGCCACTGCACGCCCTGCCGGACCTCAGCCCCAGCCCGCTCCCCCACCCCGCCATG
GGAAGGGCTCCTGTAGACTTCATCAACATCAGCACCGACATGAACACTGAGGTGGATGCC
CTCGACCCGAGCATCATGGACTTCGCTCTGCAGGGGAACCTGTGGGAGGAGATGAAGGAT
GAGGGATTCAGCTTGGACACACTGGGCGCCTTTGCAGACTCCCCGCTTGGCTGTGACCTG
GGGGCCTCAGGCCTAACCCCTGCCTCGGGTGGCAGCGACCAGTCCTTCCCAGACTTGCAG
GTGACGGGTCTCTACACAGCGTACTCCACTCCGGACAGTGTGGCTGCATCGGGCACCAGC
TCCTCCTCCCAGTACCTGGGTGCACAGGGGAACAAGCCTATAGCCCTGCTT

Protein sequence predicted based on nucleotide sequence

MIESDTSSIMSGIIRNSGQNHHPSRQEYRLLATTSDDDLPGDLQSLSWLTAVDVPRLQQM
ASGRVDLGGPCVPHPHPGALAGVADLHVGATPSPLLHGPAGMAPRGMPGLGPITGHRDSM
SQFPVGGQPSSGLQDPPHLYSPATQPQFPLPPGAQQCPPVGLYGPPFGVRPPYPQPHVAV
HSSQELHPKHYPKPIYSYSCLIAMALKNSKTGSLPVSEIYSFMKEHFPYFKTAPDGWKNS
VRHNLSLNKCFEKVENKMSGSSRKGCLWALNLARIDKMEEEMHKWKRKDLAAIHRSMANP
EELDKLISDRPESCRRPGKPGEPEAPVLTHATTVAVAHGCLAVSQLPPQPLMTLSLQSVP
LHHQVQPQAHLATDSPAPAQTPPLHALPDLSPSPLPHPAMGRAPVDFINISTDMNTEVDA
LDPSIMDFALQGNLWEEMKDEGFSLDTLGAFADSPLGCDLGASGLTPASGGSDQSFPDLQ
VTGLYTAYSTPDSVAASGTSSSSQYLGAQGNKPIALL

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

FOXN4

Symbol Nomenclature :

FOXN4

Designation :

forkhead box protein N4|forkhead/winged helix transcription factor FOXN4

Full Nomenclature :

forkhead box N4

GENEID :

121643

HGNC :

21399

MIM :

609429

Vega :

OTTHUMG00000152868

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00515888      ATGATAGAAAGTGACACCTCATCCATAATGTCAGGAATTATTCGAAACTCAGGGCAAAAT
NM_213596.2       ATGATAGAAAGTGACACCTCATCCATAATGTCAGGAATTATTCGAAACTCAGGGCAAAAT
                  ************************************************************

HsCD00515888      CACCACCCCTCTCGACAGGAATACAGGCTTCTAGCCACCACCAGCGATGATGACCTTCCC
NM_213596.2       CACCACCCCTCTCCACAGGAATACAGGCTTCTAGCCACCACCAGCGATGATGACCTTCCC
                  ************* **********************************************

HsCD00515888      GGGGACCTGCAGTCGCTGTCGTGGCTCACGGCGGTGGATGTGCCTCGGCTGCAGCAGATG
NM_213596.2       GGGGACCTGCAGTCGCTGTCGTGGCTCACGGCGGTGGATGTGCCTCGGCTGCAGCAGATG
                  ************************************************************

HsCD00515888      GCAAGTGGCCGCGTGGACCTGGGTGGCCCCTGCGTGCCACATCCACACCCAGGTGCCTTG
NM_213596.2       GCAAGTGGCCGCGTGGACCTGGGTGGCCCCTGCGTGCCACATCCACACCCAGGTGCCTTG
                  ************************************************************

HsCD00515888      GCTGGGGTGGCCGACCTGCATGTGGGAGCCACTCCAAGTCCCCTTCTCCATGGCCCAGCA
NM_213596.2       GCTGGGGTGGCCGACCTGCATGTGGGAGCCACTCCAAGTCCCCTTCTCCATGGCCCAGCA
                  ************************************************************

HsCD00515888      GGCATGGCCCCCCGAGGCATGCCAGGTCTGGGCCCCATAACTGGCCACAGAGACAGCATG
NM_213596.2       GGCATGGCCCCCCGAGGCATGCCAGGTCTGGGCCCCATAACTGGCCACAGAGACAGCATG
                  ************************************************************

HsCD00515888      AGCCAGTTCCCCGTGGGGGGCCAGCCCTCATCTGGGCTGCAGGACCCGCCGCATCTGTAC
NM_213596.2       AGCCAGTTCCCCGTGGGGGGCCAGCCCTCATCTGGCCTGCAGGACCCGCCGCATCTGTAC
                  *********************************** ************************

HsCD00515888      TCACCTGCCACCCAACCACAGTTCCCGCTCCCCCCGGGTGCCCAGCAGTGCCCTCCTGTG
NM_213596.2       TCACCTGCCACCCAACCACAGTTCCCGCTCCCCCCGGGTGCCCAGCAGTGCCCTCCTGTG
                  ************************************************************

HsCD00515888      GGCCTCTATGGCCCCCCATTTGGGGTGCGGCCCCCCTACCCCCAGCCCCACGTGGCTGTG
NM_213596.2       GGCCTCTATGGCCCCCCATTTGGGGTGCGGCCCCCCTACCCCCAGCCCCACGTGGCTGTG
                  ************************************************************

HsCD00515888      CATTCATCTCAAGAACTGCACCCCAAACACTACCCCAAGCCCATCTACTCGTACAGCTGT
NM_213596.2       CATTCATCTCAAGAACTGCACCCCAAACACTACCCCAAGCCCATCTACTCGTACAGCTGT
                  ************************************************************

HsCD00515888      CTGATCGCCATGGCCCTGAAGAACAGCAAGACAGGCAGCCTGCCTGTGAGCGAGATCTAC
NM_213596.2       CTGATCGCCATGGCCCTGAAGAACAGCAAGACAGGCAGCCTGCCTGTGAGCGAGATCTAC
                  ************************************************************

HsCD00515888      AGCTTCATGAAGGAGCACTTCCCCTACTTCAAGACGGCCCCCGACGGGTGGAAGAACTCG
NM_213596.2       AGCTTCATGAAGGAGCACTTCCCCTACTTCAAGACGGCCCCCGACGGGTGGAAGAACTCG
                  ************************************************************

HsCD00515888      GTGCGGCACAACCTGTCTCTGAACAAGTGCTTCGAGAAGGTGGAGAACAAGATGAGCGGC
NM_213596.2       GTGCGGCACAACCTGTCTCTGAACAAGTGCTTCGAGAAGGTGGAGAACAAGATGAGCGGC
                  ************************************************************

HsCD00515888      TCCTCCCGCAAGGGCTGCCTGTGGGCTCTGAACCTGGCCCGCATCGACAAGATGGAGGAG
NM_213596.2       TCCTCCCGCAAGGGCTGCCTGTGGGCTCTGAACCTGGCCCGCATCGACAAGATGGAGGAG
                  ************************************************************

HsCD00515888      GAGATGCACAAGTGGAAGAGGAAGGACCTGGCTGCCATCCACCGGAGTATGGCCAACCCT
NM_213596.2       GAGATGCACAAGTGGAAGAGGAAGGACCTGGCTGCCATCCACCGGAGTATGGCCAACCCT
                  ************************************************************

HsCD00515888      GAGGAGTTGGACAAGCTGATCTCCGACCGGCCTGAAAGCTGCCGGCGCCCCGGCAAACCG
NM_213596.2       GAGGAGTTGGACAAGCTGATCTCCGACCGGCCTGAAAGCTGCCGGCGCCCCGGCAAACCG
                  ************************************************************

HsCD00515888      GGGGAACCAGAGGCCCCCGTGCTGACTCACGCCACCACAGTGGCCGTGGCGCATGGCTGC
NM_213596.2       GGGGAACCAGAGGCCCCCGTGCTGACTCACGCCACCACAGTGGCCGTGGCGCATGGCTGC
                  ************************************************************

HsCD00515888      CTGGCTGTCTCCCAGCTCCCACCCCAGCCACTGATGACCCTGTCCCTGCAGTCAGTCCCC
NM_213596.2       CTGGCTGTCTCCCAGCTCCCACCCCAGCCACTGATGACCCTGTCCCTGCAGTCAGTCCCC
                  ************************************************************

HsCD00515888      CTGCACCACCAGGTCCAGCCCCAGGCACATCTTGCTACAGACTCTCCAGCACCAGCCCAG
NM_213596.2       CTGCACCACCAGGTCCAGCCCCAGGCACATCTTGCTCCAGACTCTCCAGCACCAGCCCAG
                  ************************************.***********************

HsCD00515888      ACCCCGCCACTGCACGCCCTGCCGGACCTCAGCCCCAGCCCGCTCCCCCACCCCGCCATG
NM_213596.2       ACCCCGCCACTGCACGCCCTGCCGGACCTCAGCCCCAGCCCGCTCCCCCACCCCGCCATG
                  ************************************************************

HsCD00515888      GGAAGGGCTCCTGTAGACTTCATCAACATCAGCACCGACATGAACACTGAGGTGGATGCC
NM_213596.2       GGAAGGGCTCCTGTAGACTTCATCAACATCAGCACCGACATGAACACTGAGGTGGATGCC
                  ************************************************************

HsCD00515888      CTCGACCCGAGCATCATGGACTTCGCTCTGCAGGGGAACCTGTGGGAGGAGATGAAGGAT
NM_213596.2       CTCGACCCGAGCATCATGGACTTCGCTCTGCAGGGGAACCTGTGGGAGGAGATGAAGGAT
                  ************************************************************

HsCD00515888      GAGGGATTCAGCTTGGACACACTGGGCGCCTTTGCAGACTCCCCGCTTGGCTGTGACCTG
NM_213596.2       GAGGGATTCAGCTTGGACACACTGGGCGCCTTTGCAGACTCCCCGCTTGGCTGTGACCTG
                  ************************************************************

HsCD00515888      GGGGCCTCAGGCCTAACCCCTGCCTCGGGTGGCAGCGACCAGTCCTTCCCAGACTTGCAG
NM_213596.2       GGGGCCTCAGGCCTAACCCCTGCCTCGGGTGGCAGCGACCAGTCCTTCCCAGACTTGCAG
                  ************************************************************

HsCD00515888      GTGACGGGTCTCTACACAGCGTACTCCACTCCGGACAGTGTGGCTGCATCGGGCACCAGC
NM_213596.2       GTGACGGGTCTCTACACAGCGTACTCCACTCCGGACAGTGTGGCTGCATCGGGCACCAGC
                  ************************************************************

HsCD00515888      TCCTCCTCCCAGTACCTGGGTGCACAGGGGAACAAGCCTATAGCCCTGCTT---
NM_213596.2       TCCTCCTCCCAGTACCTGGGTGCACAGGGGAACAAGCCTATAGCCCTGCTTTGA
                  ***************************************************