DNASU Plasmid | ZNF324B (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: ZNF324

Gene Name: zinc finger protein 324

Original Clone ID:

ccsbBroadEn_05585

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC140881.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00515923

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1633nts

Open reading frame: 1 to 1633

Reference Sequence Alignment

ATGACCTTCGAGGATGTGGCCGTGTACTTCTCCCAGGAGGAGTGGGGGCTCCTGGACACA
GCGCAGAGGGCCCTGTACCGCCACGTGATGCTGGAAAACTTCACACTTGTGACCTCACTT
GGACTCTCTACCTCCCGACCTCGTGTGGTCATTCAACTTGAGCGTGGCGAGGAGCCCTGG
GTTCCCAGTGGAAAGGACATGACCCTGGCCAGGAACACCTACGGGAGGCTCAACTCTGGT
TCCTGGAGTTTGACAGAGGATAGAGATGTTTCTGGAGAATGGCCACGAGCTTTCCCAGAT
ACCCCACCTGGGATGACTACTAGCGTCTTCCCAGTTGCCGATGCCTGCCACAGTGTAAAA
AGCCTGCAGCGACAACCGGGTGCCTCCCCATCTCAGGAGAGAAAACCCACGGGGGTGTCG
GTGATCTACTGGGAGAGGCTCCTGCTAGGCTCGCGCAGTGACCAGGCCAGCATCAGCCTG
CGACTGACCTCCCCACTCAGGCCCCCCAAGAGCAGCCGGCCCAGGGAAAAGACCTTCACA
GAGTACCGGGTGCCTGGGAGGCAGCCCAGGACGCCTGAGCGGCAGAAGCCATGTGCACAG
GAGGTCCCTGGGAGAGCCTTCGGGAATGCCTCGGACCTGAAGGCCGCCAGTGGTGGCAGG
GATCGCAGAATGGGCGCAGCTTGGCAGGAGCCTCATAGACTCCTCGGTGGCCAGGAGCCC
TCGACCTGGGACGAGCTGGGCGAGGCTCTTCACGCTGGGGAGAAGTCCTTCGAATGCAGG
GCGTGCAGCAAAGTGTTCGTGAAGAGCTCCGACCTCCTCAAGCACCTACGCACCCACACC
GGGGAGCGGCCCTACGAGTGCACCCAGTGCGGCAAGGCCTTCAGCCAGACGTCGCACTTG
ACGCAGCACCAGCGCATCCACAGCGGCGAGACGCCCTACGCGTGCCCCGTGTGCGGCAAG
GCCTTCCGGCATAGCTCCTCGCTGGTGCGGCACCAGCGCATCCACACGGCCGAGAAGTCC
TTCCGCTGCTCCGAGTGCGGCAAGGCCTTCAGCCACGGCTCCAACCTCAGCCAGCACCGC
AAGATCCACGCGGGTGGGCGTCCTTATGCTTGCGCACAGTGTGGCCGCCGCTTCTGCCGC
AACTCGCACCTGATCCAGCACGAGCGTACGCACACAGGCGAGAAGCCCTTCGTATGCGCG
CTCTGCGGTGCTGCCTTCAGCCAGGGCTCCTCGCTCTTTTTGCACCAGCGCGTGCACACA
GGCGAGAAGCCCTTCGCCTGCGCCCAGTGCGGCCGCTCCTTTAGCCGCAGCTCCAACCTC
ACCCAGCACCAGCTCCTGCACACGGGCGAGCGGCCCTTCCGCTGCGTGGACTGTGGCAAG
GGTTTCGCCAAGGGCGCCGTGCTGCTCAGCCACCGGCGCATTCACACGGGCGAGAAGCCC
TTCGTGTGCACGCAGTGTGGCCGCGCCTTCCGTGAGCGCCCTGCCCTCTTGCACCACCAG
AGGATCCACACCACAGAGAAGACCAATGCCGCAGCACCAGACTGCACCCCGGGGCCAGGT
TTCCTTCAGGGACATCATCGGAAGGTGCGCCGGGGAGGGAAGCCAAGCCCAGTCCTGAAG
CCAGCGAAGGTC

Protein sequence predicted based on nucleotide sequence

MTFEDVAVYFSQEEWGLLDTAQRALYRHVMLENFTLVTSLGLSTSRPRVVIQLERGEEPW
VPSGKDMTLARNTYGRLNSGSWSLTEDRDVSGEWPRAFPDTPPGMTTSVFPVADACHSVK
SLQRQPGASPSQERKPTGVSVIYWERLLLGSRSDQASISLRLTSPLRPPKSSRPREKTFT
EYRVPGRQPRTPERQKPCAQEVPGRAFGNASDLKAASGGRDRRMGAAWQEPHRLLGGQEP
STWDELGEALHAGEKSFECRACSKVFVKSSDLLKHLRTHTGERPYECTQCGKAFSQTSHL
TQHQRIHSGETPYACPVCGKAFRHSSSLVRHQRIHTAEKSFRCSECGKAFSHGSNLSQHR
KIHAGGRPYACAQCGRRFCRNSHLIQHERTHTGEKPFVCALCGAAFSQGSSLFLHQRVHT
GEKPFACAQCGRSFSRSSNLTQHQLLHTGERPFRCVDCGKGFAKGAVLLSHRRIHTGEKP
FVCTQCGRAFRERPALLHHQRIHTTEKTNAAAPDCTPGPGFLQGHHRKVRRGGKPSPVLK
PAKV

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

ZNF324

Symbol Nomenclature :

ZNF324

SYNONYM :

ZF5128; ZNF324A

Designation :

zinc finger protein 324A|zinc finger protein ZF5128

Full Nomenclature :

zinc finger protein 324

GENEID :

25799

HGNC :

14096

Vega :

OTTHUMG00000183540

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00515923      ATGACCTTCGAGGATGTGGCCGTGTACTTCTCCCAGGAGGAGTGGGGGCTCCTGGACACA
NM_207395.2       ATGACCTTCGAGGATGTGGCCGTGTACTTCTCCCAGGAGGAGTGGGGGCTCCTGGACACA
NM_014347.2       ATGGCCTTTGAGGATGTGGCTGTGTACTTCTCCCAGGAGGAGTGGGGGCTCCTGGACACA
                  ***.**** *********** ***************************************

HsCD00515923      GCGCAGAGGGCCCTGTACCGCCACGTGATGCTGGAAAACTTCACACTTGTGACCTCACTT
NM_207395.2       GCGCAGAGGGCCCTGTACCGCCACGTGATGCTGGAAAACTTCACACTTGTGACCTCACTT
NM_014347.2       GCCCAGAGGGCCCTGTACCGCCGCGTGATGCTAGACAACTTCGCACTTGTGGCCTCGCTG
                  ** *******************.*********.**.******.********.****.** 

HsCD00515923      GGACTCTCTACCTCCCGACCTCGTGTGGTCATTCAACTTGAGCGTGGCGAGGAGCCCTGG
NM_207395.2       GGACTCTCTACCTCCCGACCTCGTGTGGTCATTCAACTTGAGCGTGGCGAGGAGCCCTGG
NM_014347.2       GGACTCTCCACCTCTCGACCTCGTGTGGTCATCCAACTGGAGCGTGGCGAGGAGCCCTGG
                  ******** ***** ***************** ***** *********************

HsCD00515923      GTTCCCAGTGGAAAGGACATGACCCTGGCCAGGAACACCTACGGGAGGCTCAACTCTGGT
NM_207395.2       GTTCCCAGTGGAAAGGACATGACCCTGGCCAGGAACACCTACGGGAGGCTCAACTCTGGT
NM_014347.2       GTTCCCAGTGGAACGGACACAACCCTGTCCAGGACCACCTACAGGAGGCGCAACCCTGGT
                  *************.***** .****** ******.*******.****** **** *****

HsCD00515923      TCCTGGAGTTTGACAGAGGATAGAGATGTTTCTGGAGAATGGCCACGAGCTTTCCCAGAT
NM_207395.2       TCCTGGAGTTTGACAGAGGATAGAGATGTTTCTGGAGAATGGCCACGAGCTTTCCCAGAT
NM_014347.2       TCCTGGAGTTTGACAGAGGATAGAGATGTTTCTGGAGAATGGCCACGAGCTTTCCCAGAT
                  ************************************************************

HsCD00515923      ACCCCACCTGGGATGACTACTAGCGTCTTCCCAGTTGCCGATGCCTGCCACAGTGTAAAA
NM_207395.2       ACCCCACCTGGGATGACTACTAGCGTCTTCCCAGTTGCCGATGCCTGCCACAGTGTAAAA
NM_014347.2       ACCCCACCTGGGATGACTACTAGCGTCTTCCCTGTTGCCGGTGCCTGCCACAGTGTAAAA
                  ********************************:*******.*******************

HsCD00515923      AGCCTGCAGCGACAACCGGGTGCCTCCCCATCTCAGGAGAGAAAACCCACGGGGGTGTCG
NM_207395.2       AGCCTGCAGCGACAACCGGGTGCCTCCCCATCTCAGGAGAGAAAACCCACGGGGGTGTCG
NM_014347.2       AGCCTGCAGAGACAACGGGGTGCCTCCCCATCTCGGGAGAGAAAACCCACGGGGGTGTCG
                  *********.****** *****************.*************************

HsCD00515923      GTGATCTACTGGGAGAGGCTCCTGCTAGGCTCGCGCAGTGACCAGGCCAGCATCAGCCTG
NM_207395.2       GTGATCTACTGGGAGAGGCTCCTGCTAGGCTCGCGCAGTGACCAGGCCAGCATCAGCCTG
NM_014347.2       GTGATCTACTGGGAGAGGCTCCTGCTAGGCTCAGGCAGTGGGCAAGCCAGCGTCAGCCTG
                  ********************************. ******. **.******.********

HsCD00515923      CGACTGACCTCCCCACTCAGGCCCCCCAAGAGCAGCCGGCCCAGGGAAAAGACCTTCACA
NM_207395.2       CGACTGACCTCCCCACTCAGGCCCCCCAAGAGCAGCCGGCCCAGGGAAAAGACCTTCACA
NM_014347.2       CGACTGACCTCCCCGCTTAGGCCTCCCGAGGGCGTCCGGCTTAGAGAAAAGACACTCACA
                  **************.** ***** ***.**.**. *****  **.********. *****

HsCD00515923      GAGTACCGGGTGCCTGGGAGGCAGCCCAGGACGCCTGAGCGGCAGAAGCCATGTGCACAG
NM_207395.2       GAGTACCGGGTGCCTGGGAGGCAGCCCAGGACGCCTGAGCGGCAGAAGCCATGTGCACAG
NM_014347.2       GAGCATGCGTTGCTGGGGAGGCAGCCCAGGACGCCTGAGCGGCAGAAACCATGTGCACAG
                  *** *   * ***  ********************************.************

HsCD00515923      GAGGTCCCTGGGAGAGCCTTCGGGAATGCCTCGGACCTGAAGGCCGCCAGTGGTGGCAGG
NM_207395.2       GAGGTCCCTGGGAGAGCCTTCGGGAATGCCTCGGACCTGAAGGCCGCCAGTGGTGGCAGG
NM_014347.2       GAGGTCCCTGGGAGAACCTTTGGGAGCGCCCAGGACCTGGAGGCTGCCGGCGGTCGGGGA
                  ***************.**** ****. *** .*******.**** ***.* *** * .*.

HsCD00515923      GATCGCAGAATGGGCGCAGCTTGGCAGGAGCCTCATAGACTCCTCGGTGGCCAGGAGCCC
NM_207395.2       GATCGCAGAATGGGCGCAGCTTGGCAGGAGCCTCATAGACTCCTCGGTGGCCAGGAGCCC
NM_014347.2       CATCACCGAATGGGTGCAGTTTGGCAGGAGCCTCATAGACTCCTCGGTGGCCAGGAGCCC
                   ***.*.******* **** ****************************************

HsCD00515923      TCGACCTGGGACGAGCTGGGCGAGGCTCTTCACGCTGGGGAGAAGTCCTTCGAATGCAGG
NM_207395.2       TCGACCTGGGACGAGCTGGGCGAGGCTCTTCACGCTGGGGAGAAGTCCTTCGAATGCAGG
NM_014347.2       TCGACCTGGGACGAGCTGGGCGAGGCTCTTCACGCTGGGGAGAAGTCCTTCGAATGCAGG
                  ************************************************************

HsCD00515923      GCGTGCAGCAAAGTGTTCGTGAAGAGCTCCGACCTCCTCAAGCACCTACGCACCCACACC
NM_207395.2       GCGTGCAGCAAAGTGTTCGTGAAGAGCTCCGACCTCCTCAAGCACCTACGCACCCACACC
NM_014347.2       GCGTGCAGCAAAGTGTTCGTGAAGAGCTCCGACCTCCTCAAGCACCTACGCACCCACACC
                  ************************************************************

HsCD00515923      GGGGAGCGGCCCTACGAGTGCACCCAGTGCGGCAAGGCCTTCAGCCAGACGTCGCACTTG
NM_207395.2       GGGGAGCGGCCCTACGAGTGCACCCAGTGCGGCAAGGCCTTCAGCCAGACGTCGCACTTG
NM_014347.2       GGGGAGCGGCCCTACGAGTGCGCCCAGTGCGGCAAGGCCTTCAGCCAGACGTCGCACTTG
                  *********************.**************************************

HsCD00515923      ACGCAGCACCAGCGCATCCACAGCGGCGAGACGCCCTACGCGTGCCCCGTGTGCGGCAAG
NM_207395.2       ACGCAGCACCAGCGCATCCACAGCGGCGAGACGCCCTACGCGTGCCCCGTGTGCGGCAAG
NM_014347.2       ACGCAGCACCAGCGCATCCACAGCGGCGAGACGCCCTACGCGTGCCCCGTGTGCGGCAAG
                  ************************************************************

HsCD00515923      GCCTTCCGGCATAGCTCCTCGCTGGTGCGGCACCAGCGCATCCACACGGCCGAGAAGTCC
NM_207395.2       GCCTTCCGGCATAGCTCCTCGCTGGTGCGGCACCAGCGCATCCACACGGCCGAGAAGTCC
NM_014347.2       GCCTTCCGGCATAGCTCCTCGCTGGTGCGGCACCAGCGCATCCACACGGCCGAGAAGTCC
                  ************************************************************

HsCD00515923      TTCCGCTGCTCCGAGTGCGGCAAGGCCTTCAGCCACGGCTCCAACCTCAGCCAGCACCGC
NM_207395.2       TTCCGCTGCTCCGAGTGCGGCAAGGCCTTCAGCCACGGCTCCAACCTCAGCCAGCACCGC
NM_014347.2       TTCCGCTGCTCCGAGTGCGGCAAGGCCTTCAGCCACGGCTCCAACCTCAGCCAGCACCGC
                  ************************************************************

HsCD00515923      AAGATCCACGCGGGTGGGCGTCCTTATGCTTGCGCACAGTGTGGCCGCCGCTTCTGCCGC
NM_207395.2       AAGATCCACGCGGGTGGGCGTCCTTATGCTTGCGCACAGTGTGGCCGCCGCTTCTGCCGC
NM_014347.2       AAGATCCACGCGGGTGGGCGTCCTTATGCTTGCGCACAGTGTGGCCGCCGCTTCTGCCGC
                  ************************************************************

HsCD00515923      AACTCGCACCTGATCCAGCACGAGCGTACGCACACAGGCGAGAAGCCCTTCGTATGCGCG
NM_207395.2       AACTCGCACCTGATCCAGCACGAGCGTACGCACACAGGCGAGAAGCCCTTCGTATGCGCG
NM_014347.2       AACTCGCACCTGATCCAGCACGAGCGTACGCACACAGGCGAGAAGCCCTTCGTGTGCGCG
                  *****************************************************.******

HsCD00515923      CTCTGCGGTGCTGCCTTCAGCCAGGGCTCCTCGCTCTTTTTGCACCAGCGCGTGCACACA
NM_207395.2       CTCTGCGGTGCTGCCTTCAGCCAGGGCTCCTCGCTCTTTTTGCACCAGCGCGTGCACACA
NM_014347.2       CTCTGCGGTGCTGCCTTCAGCCAGGGCTCCTCGCTCTTTAAGCACCAGCGCGTGCACACA
                  ***************************************::*******************

HsCD00515923      GGCGAGAAGCCCTTCGCCTGCGCCCAGTGCGGCCGCTCCTTTAGCCGCAGCTCCAACCTC
NM_207395.2       GGCGAGAAGCCCTTCGCCTGCGCCCAGTGCGGCCGCTCCTTTAGCCGCAGCTCCAACCTC
NM_014347.2       GGCGAGAAGCCCTTCGCCTGCCCACAGTGCGGCCGCGCCTTTAGCCACAGCTCCAACCTC
                  ********************* *.************ *********.*************

HsCD00515923      ACCCAGCACCAGCTCCTGCACACGGGCGAGCGGCCCTTCCGCTGCGTGGACTGTGGCAAG
NM_207395.2       ACCCAGCACCAGCTCCTGCACACGGGCGAGCGGCCCTTCCGCTGCGTGGACTGTGGCAAG
NM_014347.2       ACCCAGCACCAGCTCCTGCACACGGGCGAGCGGCCCTTCCGCTGCGTGGACTGTGGCAAG
                  ************************************************************

HsCD00515923      GGTTTCGCCAAGGGCGCCGTGCTGCTCAGCCACCGGCGCATTCACACGGGCGAGAAGCCC
NM_207395.2       GGTTTCGCCAAGGGCGCCGTGCTGCTCAGCCACCGGCGCATTCACACGGGCGAGAAGCCC
NM_014347.2       GCCTTCGCCAAGGGCGCCGTGCTGCTCAGCCACCGGCGCATTCACACGGGCGAGAAGCCC
                  *  *********************************************************

HsCD00515923      TTCGTGTGCACGCAGTGTGGCCGCGCCTTCCGTGAGCGCCCTGCCCTCTTGCACCACCAG
NM_207395.2       TTCGTGTGCACGCAGTGTGGCCGCGCCTTCCGTGAGCGCCCTGCCCTCTTGCACCACCAG
NM_014347.2       TTCGTGTGTACGCAGTGTGGCCGCGCCTTCCGTGAGCGCCCGGCCCTCTTCCACCACCAG
                  ******** ******************************** ******** *********

HsCD00515923      AGGATCCACACCACAGAGAAGACCAATG----CCGCAGCACCAGACTGCACCCCGGGGCC
NM_207395.2       AGGATCCACACCACAGAGAAGACCAATG----CCGCAGCACCAGACTGCACCCCGGGGCC
NM_014347.2       AGGATCCATACCGGCGAGAAGACCGTCCGGCGATCCAGGGCCAGCCTGCACCCCCAGGCC
                  ******** ***. .*********.:      .  *** .****.********* .****

HsCD00515923      AGGTTTCCTTCAGGGACATCATCGGAAGGTGCGCCGGGGAGGGAAGCCAAGCCCAGTCCT
NM_207395.2       AGGTTTCCTTCAGGGACATCATCGGAAGGTGCGCCGGGGAGGGAAGCCAAGCCCAGTCCT
NM_014347.2       AGGTCTGTTGCCGGGGCATCATCAGAAGGTGCGCCAGCGAAGGAAACCGAGCCCACTCCC
                  **** *  * *.***.*******.***********.* **.****.**.****** *** 

HsCD00515923      GAAGCCAGCGAAGGTC--------------------------
NM_207395.2       GAAGCCAGCGAAGGTCTGA-----------------------
NM_014347.2       GCCTCGGGCCCAGCCGCCGTCTCGCAGCCAGCGGAGGTCTGA
                  *.. * .** .**