DNASU Plasmid | MSL2 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: MSL2

Gene Name: male-specific lethal 2 homolog (Drosophila)

Original Clone ID:

ccsbBroadEn_03540

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC093790.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00515952

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1732nts

Open reading frame: 1 to 1732

Reference Sequence Alignment

ATGAACCCCGTGAATGCTACTGCTCTCTACATTTCCGCGAGCCGCCTAGTGCTCAACTAC
GACCCCGGAGACCCCAAGGCGTTTACTGAGATTAACAGGCTCTTGCCTTACTTCCGACAG
TCCCTTTCGTGCTGTGTTTGCGGACATTTGCTACAAGATCCTATTGCACCCACCAACTCC
ACCTGCCAACATTATGTCTGCAAAACTTGTAAAGGCAAGAAAATGATGATGAAACCTTCC
TGTAGCTGGTGCAAAGACTATGAGCAGTTTGAGGAAAACAAGCAGTTAAGCATCCTAGTG
AACTGCTACAAAAAACTATGCGAGTATATAACACAGACTACACTGGCACGGGATATAATA
GAAGCAGTTGACTGTTCTTCTGATATTTTGGCTTTGCTTAATGATGGATCATTGTTTTGT
GAGGAGACAGAAAAACCCTCAGATTCATCCTTTACTTTGTGTTTGACACATTCCCCTTTA
CCTTCAACCTCAGAACCCACAACTGATCCTCAAGCTAGTTTATCTCCAATGTCTGAAAGC
ACCCTCAGCATTGCTATTGGCAGTTCTGTTATCAATGGTTTGCCTACTTATAATGGGCTT
TCAATAGATAGATTTGGTATAAATATTCCTTCACCTGAACATTCAAATACGATTGACGTA
TGTAATACTGTTGACATAAAAACTGAGGATCTGTCTGACAGCCTGCCACCCGTTTGTGAC
ACAGTAGCCACTGACTTATGTTCCACAGGCATTGATATCTGCAGTTTCAGTGAAGATATA
AAACCTGGAGACTCTCTGTTACTGAGTGTTGAGGAAGTACTCCGCAGCTTAGAAACTGTT
TCAAATACAGAGGTCTGTTGCCCTAATTTGCAGCCGAACTTGGAAGCCACTGTATCCAAT
GGACCTTTTCTGCAGCTTTCTTCCCAGTCTCTTAGCCATAATGTTTTTATGTCCACCAGT
CCTGCACTTCATGGGTTATCATGTACAGCAGCAACTCCGAAGATAGCAAAATTGAATAGA
AAACGATCCAGATCAGAGAGTGACAGTGAGAAAGTTCAGCCACTTCCAATTTCTACCATT
ATCCGAGGCCCAACACTGGGGGCATCTGCTCCTGTGACAGTGAAACGGGAGAGCAAAATT
TCTCTTCAACCTATAGCAACTGTTCCCAATGGAGGCACAACACCTAAAATCAGCAAAACT
GTACTTTTATCTACTAAAAGCATGAAAAAGAGTCATGAACATGGATCCAAGAAATCTCAC
TCTAAAACCAAGCCAGGTATTCTTAAAAAAGACAAAGCAGTAAAGGAAAAGATTCCTAGT
CATCATTTTATGCCAGGAAGTCCTACCAAGACTGTGTACAAAAAACCCCAGGAAAAGAAA
GGGTGTAAATGTGGGCGTGCTACTCAAAATCCAAGTGTTCTTACATGCCGAGGCCAACGC
TGCCCTTGCTACTCTAACCGCAAAGCCTGCTTAGATTGTATATGTCGTGGCTGCCAAAAC
TCCTATATGGCCAATGGGGAGAAGAAGCTGGAGGCATTTGCCGTGCCAGAAAAGGCCTTG
GAGCAGACCAGGCTCACTTTGGGCATTAACGTGACTAGCATTGCTGTGCGTAACGCTAGT
ACCAGCACCAGTGTAATAAATGTCACAGGGTCCCCAGTAACGACGTTTTTAGCTGCCAGT
ACACATGATGATAAAAGTTTGGATGAAGCTATAGACATGAGATTCGACTGT

Protein sequence predicted based on nucleotide sequence

MNPVNATALYISASRLVLNYDPGDPKAFTEINRLLPYFRQSLSCCVCGHLLQDPIAPTNS
TCQHYVCKTCKGKKMMMKPSCSWCKDYEQFEENKQLSILVNCYKKLCEYITQTTLARDII
EAVDCSSDILALLNDGSLFCEETEKPSDSSFTLCLTHSPLPSTSEPTTDPQASLSPMSES
TLSIAIGSSVINGLPTYNGLSIDRFGINIPSPEHSNTIDVCNTVDIKTEDLSDSLPPVCD
TVATDLCSTGIDICSFSEDIKPGDSLLLSVEEVLRSLETVSNTEVCCPNLQPNLEATVSN
GPFLQLSSQSLSHNVFMSTSPALHGLSCTAATPKIAKLNRKRSRSESDSEKVQPLPISTI
IRGPTLGASAPVTVKRESKISLQPIATVPNGGTTPKISKTVLLSTKSMKKSHEHGSKKSH
SKTKPGILKKDKAVKEKIPSHHFMPGSPTKTVYKKPQEKKGCKCGRATQNPSVLTCRGQR
CPCYSNRKACLDCICRGCQNSYMANGEKKLEAFAVPEKALEQTRLTLGINVTSIAVRNAS
TSTSVINVTGSPVTTFLAASTHDDKSLDEAIDMRFDC

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

MSL2

Symbol Nomenclature :

MSL2

SYNONYM :

MSL-2; MSL2L1; RNF184

Designation :

E3 ubiquitin-protein ligase MSL2|MSL2-like 1|male-specific lethal 2-like 1|male-specific lethal-2 homolog 1|ring finger protein 184

Full Nomenclature :

male-specific lethal 2 homolog (Drosophila)

GENEID :

55167

HGNC :

25544

MIM :

614802

Vega :

OTTHUMG00000159793

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00515952      ATGAACCCCGTGAATGCTACTGCTCTCTACATTTCCGCGAGCCGCCTAGTGCTCAACTAC
NM_018133.3       ATGAACCCCGTGAATGCTACTGCTCTCTACATTTCCGCGAGCCGCCTAGTGCTCAACTAC
                  ************************************************************

HsCD00515952      GACCCCGGAGACCCCAAGGCGTTTACTGAGATTAACAGGCTCTTGCCTTACTTCCGACAG
NM_018133.3       GACCCCGGAGACCCCAAGGCGTTTACTGAGATTAACAGGCTCTTGCCTTACTTCCGACAG
                  ************************************************************

HsCD00515952      TCCCTTTCGTGCTGTGTTTGCGGACATTTGCTACAAGATCCTATTGCACCCACCAACTCC
NM_018133.3       TCCCTTTCGTGCTGTGTTTGCGGACATTTGCTACAAGATCCTATTGCACCCACCAACTCC
                  ************************************************************

HsCD00515952      ACCTGCCAACATTATGTCTGCAAAACTTGTAAAGGCAAGAAAATGATGATGAAACCTTCC
NM_018133.3       ACCTGCCAACATTATGTCTGCAAAACTTGTAAAGGCAAGAAAATGATGATGAAACCTTCC
                  ************************************************************

HsCD00515952      TGTAGCTGGTGCAAAGACTATGAGCAGTTTGAGGAAAACAAGCAGTTAAGCATCCTAGTG
NM_018133.3       TGTAGCTGGTGCAAAGACTATGAGCAGTTTGAGGAAAACAAGCAGTTAAGCATCCTAGTG
                  ************************************************************

HsCD00515952      AACTGCTACAAAAAACTATGCGAGTATATAACACAGACTACACTGGCACGGGATATAATA
NM_018133.3       AACTGCTACAAAAAACTATGCGAGTATATAACACAGACTACACTGGCACGGGATATAATA
                  ************************************************************

HsCD00515952      GAAGCAGTTGACTGTTCTTCTGATATTTTGGCTTTGCTTAATGATGGATCATTGTTTTGT
NM_018133.3       GAAGCAGTTGACTGTTCTTCTGATATTTTGGCTTTGCTTAATGATGGATCATTGTTTTGT
                  ************************************************************

HsCD00515952      GAGGAGACAGAAAAACCCTCAGATTCATCCTTTACTTTGTGTTTGACACATTCCCCTTTA
NM_018133.3       GAGGAGACAGAAAAACCCTCAGATTCATCCTTTACTTTGTGTTTGACACATTCCCCTTTA
                  ************************************************************

HsCD00515952      CCTTCAACCTCAGAACCCACAACTGATCCTCAAGCTAGTTTATCTCCAATGTCTGAAAGC
NM_018133.3       CCTTCAACCTCAGAACCCACAACTGATCCTCAAGCTAGTTTATCTCCAATGTCTGAAAGC
                  ************************************************************

HsCD00515952      ACCCTCAGCATTGCTATTGGCAGTTCTGTTATCAATGGTTTGCCTACTTATAATGGGCTT
NM_018133.3       ACCCTCAGCATTGCTATTGGCAGTTCTGTTATCAATGGTTTGCCTACTTATAATGGGCTT
                  ************************************************************

HsCD00515952      TCAATAGATAGATTTGGTATAAATATTCCTTCACCTGAACATTCAAATACGATTGACGTA
NM_018133.3       TCAATAGATAGATTTGGTATAAATATTCCTTCACCTGAACATTCAAATACGATTGACGTA
                  ************************************************************

HsCD00515952      TGTAATACTGTTGACATAAAAACTGAGGATCTGTCTGACAGCCTGCCACCCGTTTGTGAC
NM_018133.3       TGTAATACTGTTGACATAAAAACTGAGGATCTGTCTGACAGCCTGCCACCCGTTTGTGAC
                  ************************************************************

HsCD00515952      ACAGTAGCCACTGACTTATGTTCCACAGGCATTGATATCTGCAGTTTCAGTGAAGATATA
NM_018133.3       ACAGTAGCCACTGACTTATGTTCCACAGGCATTGATATCTGCAGTTTCAGTGAAGATATA
                  ************************************************************

HsCD00515952      AAACCTGGAGACTCTCTGTTACTGAGTGTTGAGGAAGTACTCCGCAGCTTAGAAACTGTT
NM_018133.3       AAACCTGGAGACTCTCTGTTACTGAGTGTTGAGGAAGTACTCCGCAGCTTAGAAACTGTT
                  ************************************************************

HsCD00515952      TCAAATACAGAGGTCTGTTGCCCTAATTTGCAGCCGAACTTGGAAGCCACTGTATCCAAT
NM_018133.3       TCAAATACAGAGGTCTGTTGCCCTAATTTGCAGCCGAACTTGGAAGCCACTGTATCCAAT
                  ************************************************************

HsCD00515952      GGACCTTTTCTGCAGCTTTCTTCCCAGTCTCTTAGCCATAATGTTTTTATGTCCACCAGT
NM_018133.3       GGACCTTTTCTGCAGCTTTCTTCCCAGTCTCTTAGCCATAATGTTTTTATGTCCACCAGT
                  ************************************************************

HsCD00515952      CCTGCACTTCATGGGTTATCATGTACAGCAGCAACTCCGAAGATAGCAAAATTGAATAGA
NM_018133.3       CCTGCACTTCATGGGTTATCATGTACAGCAGCAACTCCGAAGATAGCAAAATTGAATAGA
                  ************************************************************

HsCD00515952      AAACGATCCAGATCAGAGAGTGACAGTGAGAAAGTTCAGCCACTTCCAATTTCTACCATT
NM_018133.3       AAACGATCCAGATCAGAGAGTGACAGTGAGAAAGTTCAGCCACTTCCAATTTCTACCATT
                  ************************************************************

HsCD00515952      ATCCGAGGCCCAACACTGGGGGCATCTGCTCCTGTGACAGTGAAACGGGAGAGCAAAATT
NM_018133.3       ATCCGAGGCCCAACACTGGGGGCATCTGCTCCTGTGACAGTGAAACGGGAGAGCAAAATT
                  ************************************************************

HsCD00515952      TCTCTTCAACCTATAGCAACTGTTCCCAATGGAGGCACAACACCTAAAATCAGCAAAACT
NM_018133.3       TCTCTTCAACCTATAGCAACTGTTCCCAATGGAGGCACAACACCTAAAATCAGCAAAACT
                  ************************************************************

HsCD00515952      GTACTTTTATCTACTAAAAGCATGAAAAAGAGTCATGAACATGGATCCAAGAAATCTCAC
NM_018133.3       GTACTTTTATCTACTAAAAGCATGAAAAAGAGTCATGAACATGGATCCAAGAAATCTCAC
                  ************************************************************

HsCD00515952      TCTAAAACCAAGCCAGGTATTCTTAAAAAAGACAAAGCAGTAAAGGAAAAGATTCCTAGT
NM_018133.3       TCTAAAACCAAGCCAGGTATTCTTAAAAAAGACAAAGCAGTAAAGGAAAAGATTCCTAGT
                  ************************************************************

HsCD00515952      CATCATTTTATGCCAGGAAGTCCTACCAAGACTGTGTACAAAAAACCCCAGGAAAAGAAA
NM_018133.3       CATCATTTTATGCCAGGAAGTCCTACCAAGACTGTGTACAAAAAACCCCAGGAAAAGAAA
                  ************************************************************

HsCD00515952      GGGTGTAAATGTGGGCGTGCTACTCAAAATCCAAGTGTTCTTACATGCCGAGGCCAACGC
NM_018133.3       GGGTGTAAATGTGGGCGTGCTACTCAAAATCCAAGTGTTCTTACATGCCGAGGCCAACGC
                  ************************************************************

HsCD00515952      TGCCCTTGCTACTCTAACCGCAAAGCCTGCTTAGATTGTATATGTCGTGGCTGCCAAAAC
NM_018133.3       TGCCCTTGCTACTCTAACCGCAAAGCCTGCTTAGATTGTATATGTCGTGGCTGCCAAAAC
                  ************************************************************

HsCD00515952      TCCTATATGGCCAATGGGGAGAAGAAGCTGGAGGCATTTGCCGTGCCAGAAAAGGCCTTG
NM_018133.3       TCCTATATGGCCAATGGGGAGAAGAAGCTGGAGGCATTTGCCGTGCCAGAAAAGGCCTTG
                  ************************************************************

HsCD00515952      GAGCAGACCAGGCTCACTTTGGGCATTAACGTGACTAGCATTGCTGTGCGTAACGCTAGT
NM_018133.3       GAGCAGACCAGGCTCACTTTGGGCATTAACGTGACTAGCATTGCTGTGCGTAACGCTAGT
                  ************************************************************

HsCD00515952      ACCAGCACCAGTGTAATAAATGTCACAGGGTCCCCAGTAACGACGTTTTTAGCTGCCAGT
NM_018133.3       ACCAGCACCAGTGTAATAAATGTCACAGGGTCCCCAGTAACGACGTTTTTAGCTGCCAGT
                  ************************************************************

HsCD00515952      ACACATGATGATAAAAGTTTGGATGAAGCTATAGACATGAGATTCGACTGT---
NM_018133.3       ACACATGATGATAAAAGTTTGGATGAAGCTATAGACATGAGATTCGACTGTTAA
                  ***************************************************