Gene:
Gene Symbol:
ZNF574
Gene Name:
zinc finger protein 574
Original Clone ID:
ccsbBroadEn_03962
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC001184.1
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00516051
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGACTGAGGAATCAGAGGAGACAGTCCTGTACATTGAGCACCGCTATGTCTGCTCTGAG
TGCAACCAGCTGTATGGATCACTGGAAGAGGTGCTTATGCACCAAAACTCCCACGTGCCC
CAGCAGCACTTTGAGCTGGTGGGCGTGGCTGATCCCGGAGTCACTGTGGCCACAGACACA
GCTTCAGGCACGGGCCTCTATCAGACCCTTGTGCAGGAGAGCCAGTACCAGTGCCTGGAG
TGTGGTCAACTGCTGATGTCACCCAGCCAGCTCCTGGAGCACCAGGAGCTGCACCTGAAG
ATGATGGCACCCCAGGAGGCAGTGCCAGCTGAGCCATCACCTAAGGCACCACCCCTGAGC
TCCAGCACCATCCACTACGAGTGTGTGGATTGCAAGGCTCTCTTTGCCAGCCAGGAGCTC
TGGCTGAACCACCGGCAGACGCACCTCCGGGCCACACCCACCAAGGCTCCTGCCCCTGTT
GTCCTGGGGTCCCCAGTTGTTCTAGGGCCTCCTGTGGGCCAGGCCCGAGTGGCTGTGGAG
CACTCATACCGAAAGGCAGAAGAGGGTGGGGAAGGGGCGACTGTCCCATCTGCCGCTGCC
ACCACCACTGAGGTAGTGACTGAGGTGGAGCTGCTCCTCTACAAGTGCTCTGAGTGCTCC
CAGCTCTTCCAGCTGCCGGCGGATTTCCTGGAGCACCAGGCCACTCACTTCCCTGCTCCT
GTACCCGAGTCTCAGGAGCCTGCCTTACAGCAGGAGGTGCAGGCCTCGTCACCTGCAGAG
GTGCCTGTGTCTCAGCCTGACCCCTTGCCAGCTTCTGACCACAGTTACGAGCTGCGCAAT
GGTGAAGCCATTGGGCGGGATCGCCGGGGGCGCAGGGCCCGGAGGAACAACAGTGGAGAA
GCAGGCGGGGCAGCCACACAGGAGCTCTTCTGCTCAGCCTGTGACCAGCTCTTTCTCTCA
CCCCACCAGCTACAGCAGCACCTGCGGAGTCACCGGGAGGGCGTCTTTAAGTGCCCCCTG
TGCAGTCGTGTCTTCCCTAGCCCTTCCAGTCTGGACCAGCACCTTGGAGACCATAGCAGC
GAGTCACACTTCCTGTGTGTAGACTGTGGCCTGGCCTTCGGCACAGAGGCCCTCCTCCTG
GCCCACCGGCGAGCCCACACCCCGAATCCTCTGCATTCATGTCCATGTGGGAAGACCTTT
GTCAACCTTACCAAGTTCCTTTATCACCGGCGTACTCATGGGGTAGGGGGTGTCCCTCTG
CCCACAACACCAGTCCCACCAGAGGAACCTGTCATTGGTTTCCCTGAGCCAGCCCCAGCA
GAGACTGGAGAGCCAGAGGCCCCTGAGCCCCCTGTGTCTGAGGAGACCTCAGCAGGGCCC
GCTGCCCCAGGCACCTACCGCTGCCTCCTGTGCAGCCGTGAATTTGGAAAGGCCTTGCAG
CTGACCCGGCACCAACGTTTTGTGCATCGGCTGGAGCGGCGCCATAAATGCAGCATTTGT
GGCAAGATGTTCAAGAAGAAGTCTCACGTGCGTAACCACCTGCGCACACACACAGGGGAG
CGGCCCTTCCCCTGCCCTGACTGCTCCAAGCCCTTCAACTCACCTGCCAACCTGGCCCGC
CACCGGCTCACACACACAGGAGAGCGGCCCTACCGGTGTGGGGACTGTGGCAAGGCTTTC
ACGCAAAGCTCCACACTGAGGCAGCACCGCTTGGTGCATGCCCAGCACTTCCCCTACCGC
TGCCAGGAATGTGGGGTGCGTTTTCACCGTCCTTACCGCCTGCTCATGCACCGCTACCAT
CACACAGGTGAATACCCCTACAAGTGTCGCGAGTGCCCCCGCTCCTTCTTGCTGCGTCGG
CTGCTGGAGGTGCACCAGCTCGTGGTCCATGCCGGGCGCCAGCCCCACCGCTGCCCATCC
TGTGGGGCTGCCTTCCCCTCCTCACTGCGGCTCCGGGAGCACCGCTGTGCAGCCGCTGCT
GCCCAGGCCCCACGGCGCTTTGAGTGTGGCACCTGTGGCAAGAAAGTGGGCTCAGCTGCT
CGACTGCAGGCACACGAGGCGGCCCATGCAGCTGCTGGGCCTGGAGAGGTCCTGGCTAAG
GAGCCCCCTGCCCCTCGAGCCCCACGGGCCACTCGTGCACCAGTTGCCTCTCCAGCAGCC
CTTGGAAGCACTGCTACAGCATCCCCTGCGGCCCCTGCCCGCCGCCGGGGTCTAGAGTGC
AGCGAGTGCAAGAAGCTGTTCAGCACAGAGACGTCACTGCAGGTGCACCGGCGCATCCAC
ACAGGTGAGCGGCCATACCCATGTCCAGACTGTGGCAAAGCGTTCCGTCAGAGTACCCAC
CTGAAAGACCACCGGCGCCTGCACACAGGTGAGCGGCCCTTTGCCTGTGAAGTGTGTGGC
AAGGCCTTTGCCATCTCCATGCGCCTGGCAGAACATCGCCGCATCCACACAGGCGAACGA
CCCTACTCCTGCCCTGACTGTGGCAAGAGCTACCGCTCCTTCTCCAACCTCTGGAAGCAC
CGCAAGACCCATCAGCAGCAGCATCAGGCAGCTGTGCGGCAGCAGCTGGCAGAGGCGGAG
GCTGCCGTTGGCCTGGCCGTCATGGAGACTGCTGTGGAGGCGCTACCCCTGGTGGAAGCC
ATTGAGATCTACCCTCTGGCCGAGGCTGAGGGGGTCCAGATCAGTGGC
Protein sequence predicted based on nucleotide sequence
MTEESEETVLYIEHRYVCSECNQLYGSLEEVLMHQNSHVPQQHFELVGVADPGVTVATDT ASGTGLYQTLVQESQYQCLECGQLLMSPSQLLEHQELHLKMMAPQEAVPAEPSPKAPPLS SSTIHYECVDCKALFASQELWLNHRQTHLRATPTKAPAPVVLGSPVVLGPPVGQARVAVE HSYRKAEEGGEGATVPSAAATTTEVVTEVELLLYKCSECSQLFQLPADFLEHQATHFPAP VPESQEPALQQEVQASSPAEVPVSQPDPLPASDHSYELRNGEAIGRDRRGRRARRNNSGE AGGAATQELFCSACDQLFLSPHQLQQHLRSHREGVFKCPLCSRVFPSPSSLDQHLGDHSS ESHFLCVDCGLAFGTEALLLAHRRAHTPNPLHSCPCGKTFVNLTKFLYHRRTHGVGGVPL PTTPVPPEEPVIGFPEPAPAETGEPEAPEPPVSEETSAGPAAPGTYRCLLCSREFGKALQ LTRHQRFVHRLERRHKCSICGKMFKKKSHVRNHLRTHTGERPFPCPDCSKPFNSPANLAR HRLTHTGERPYRCGDCGKAFTQSSTLRQHRLVHAQHFPYRCQECGVRFHRPYRLLMHRYH HTGEYPYKCRECPRSFLLRRLLEVHQLVVHAGRQPHRCPSCGAAFPSSLRLREHRCAAAA AQAPRRFECGTCGKKVGSAARLQAHEAAHAAAGPGEVLAKEPPAPRAPRATRAPVASPAA LGSTATASPAAPARRRGLECSECKKLFSTETSLQVHRRIHTGERPYPCPDCGKAFRQSTH LKDHRRLHTGERPFACEVCGKAFAISMRLAEHRRIHTGERPYSCPDCGKSYRSFSNLWKH RKTHQQQHQAAVRQQLAEAEAAVGLAVMETAVEALPLVEAIEIYPLAEAEGVQISG
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223
Synonyms:
pDONR223, pENTR223-XnCDSYn
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp):
2815
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Mark Vidal
Publications:
PMID:
15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
bacterial origin of replication
2220
2753
gene fragment
pas
5p stem of pBR primose assembly site
63
73
gene fragment
pas prime
3p stem of pBR pas(L)
105
116
recombination site
attL1
attL1 recombination site
570
665
recombination site
attL2
attL2 recombination site
793
696
selectable marker
SpnR
spectinomycin resistance gene
1062
2072
trxn termination sequence
T1-T2
rrnBT1 and T2 terminators
510
224
