Gene:
Gene Symbol:
MYOF
Gene Name:
myoferlin
Original Clone ID:
ccsbBroadEn_11828
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC052617.1
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00516079
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGCTGCGAGTGATTGTGGAATCTGCCAGCAATATCCCTAAAACGAAATTTGGCAAGCCG
GATCCTATTGTTTCTGTCATTTTTAAGGATGAGAAAAAGAAAACAAAGAAAGTTGATAAT
GAATTGAACCCTGTCTGGAATGAGATTTTGGAGTTTGACTTGAGGGGTATACCACTGGAC
TTTTCATCTTCCCTTGGGATTATTGTGAAAGATTTTGAGACAATTGGACAAAATAAATTA
ATTGGCACGGCGACTGTAGCCCTGAAGGACCTGACTGGTGACCAGAGCAGATCCCTGCCG
TACAAGCTGATCTCCCTGCTAAATGAAAAAGGGCAAGATACTGGGGCCACCATTGACTTG
GTGATCGGCTATGATCCGCCTTCTGCTCCACATCCAAATGACCTGAGCGGGCCCAGCGTG
CCAGGCATGGGAGGAGATGGGGAAGAAGATGAAGGTGATGAAGACAGGTTGGACAATGCA
GTCAGGGGCCCTGGGCCCAAGGGGCCAGTTGGGACGGTGTCGGAAGCTCAGCTTGCTCGG
AGGCTCACCAAAGTAAAGAACAGCCGGCGGATGCTGTCAAATAAGCCACAGGACTTCCAG
ATCCGCGTCCGAGTGATTGAGGGCCGACAGTTAAGTGGTAACAACATAAGGCCTGTGGTC
AAAGTTCACGTCTGTGGCCAGACACACCGAACAAGAATCAAGAGAGGAAACAACCCTTTT
TTTGATGAGTTGTTTTTCTACAATGTCAACATGACCCCTTCTGAATTGATGGATGAGATC
ATCAGCATCCGGGTTTATAATTCTCACTCTCTGCGGGCAGATTGTCTGATGGGGGAATTT
AAGATTGATGTTGGATTTGTTTATGATGAACCTGGCCATGCTGTCATGAGAAAGTGGCTT
CTTCTCAATGACCCGGAAGATACCAGTTCAGGTTCTAAAGGTTATATGAAAGTCAGCATG
TTTGTCCTGGGAACCGGAGATGAGCCTCCTCCTGAGAGACGAGATCGTGATAATGACAGT
GATGATGTGGAGAGTAATTTGTTACTCCCTGCTGGCATTGCCCTCCGGTGGGTGACCTTC
TTGCTGAAAATCTACCGAGCTGAGGACATCCCCCAGATGGATGATGCCTTCTCACAGACA
GTAAAGGAAATATTTGGAGGCAATGCAGATAAGAAAAATCTCGTGGATCCTTTTGTAGAA
GTTTCCTTTGCTGGAAAAAAGGTTTGTACAAACATAATTGAGAAAAATGCAAACCCAGAG
TGGAATCAGGTCGTCAATCTTCAGATCAAGTTTCCTTCAGTGTGTGAAAAAATAAAACTA
ACAATATATGACTGGGACCGTCTTACTAAAAATGATGTAGTTGGAACAACATATCTACAC
CTCTCTAAAATTGCTGCCTCTGGTGGGGAAGTGGAAGATTTCTCATCTTCGGGAACTGGG
GCTGCATCATATACAGTAAACACAGGAGAAACAGAGGTAGGCTTTGTTCCAACGTTTGGA
CCTTGTTACCTGAATCTTTATGGAAGCCCCAGAGAGTACACGGGATTCCCAGACCCCTAT
GATGAGCTGAATACTGGAAAGGGGGAAGGAGTTGCCTACAGAGGCAGGATCTTGGTTGAA
TTAGCCACTTTTCTTGAGAAGACACCACCAGATAAAAAGCTTGAGCCCATTTCAAATGAT
GACCTGCTGGTTGTTGAGAAATACCAGCGAAGGCGGAAGTACAGCCTGTCTGCCGTGTTT
CATTCAGCCACCATGTTGCAAGATGTTGGTGAGGCCATTCAGTTTGAAGTCAGCATTGGG
AACTATGGCAACAAGTTTGACACCACCTGTAAGCCTTTGGCATCAACAACTCAGTACAGC
CGTGCTGTATTTGATGGCAACTACTATTATTACTTGCCTTGGGCCCACACCAAGCCAGTT
GTTACCCTGACTTCATACTGGGAGGATATTAGTCATCGCCTGGATGCGGTGAACACTCTC
CTAGCTATGGCAGAACGGCTGCAAACAAATATAGAAGCTCTAAAATCAGGGATACAAGGT
AAAATTCCTGCAAACCAGCTGGCTGAATTGTGGCTGAAGCTGATAGATGAAGTTATAGAA
GACACGAGATACACGTTGCCTCTCACAGAAGGAAAAGCCAACGTCACAGTTCTCGATACT
CAGATCCGAAAGCTGCGGTCCAGGTCTCTCTCCCAAATACATGAGGCGGCTGTGAGGATG
AGGTCGGAAGCCACAGATGTGAAGTCCACACTGGCAGAAATTGAGGACTGGCTTGATAAA
TTAATGCAGCTGACTGAAGAGCCACAGAACAGCATGCCTGACATCATCATCTGGATGATC
CGGGGAGAGAAGAGACTGGCCTATGCACGAATTCCCGCACATCAGGTCTTGTACTCCACC
AGTGGTGAGAATGCATCTGGAAAATACTGTGGGAAAACCCAAACCATCTTTCTGAAGTAT
CCACAGGAGAAAAACAACGGGCCAAAGGTGCCTGTGGAGTTGCGAGTGAACATCTGGCTA
GGCTTAAGTGCTGTGGAGAAGAAGTTTAACAGCTTCGCAGAAGGAACTTTCACCGTCTTT
GCTGAAATGTATGAAAATCAAGCTCTCATGTTTGGAAAATGGGGTACTTCTGGATTAGTA
GGACGTCATAAGTTTTCTGATGTCACAGGAAAAATAAAACTCAAGAGGGAATTTTTTCTG
CCTCCAAAAGGCTGGGAATGGGAAGGAGAGTGGATAGTTGATCCTGAAAGAAGCTTGCTG
ACTGAGGCAGATGCAGGTCACACGGAGTTCACTGATGAAGTCTATCAGAACGAGAGCCGC
TACCCCGGGGGCGACTGGAAGCCGGCCGAGGACACCTACACGGATGCGAACGGCGATAAA
GCAGCATCACCCAGCGAGTTGACTTGTCCTCCAGGTTGGGAATGGGAAGATGATGCATGG
TCTTATGACATAAATCGAGCGGTGGATGAGAAAGGCTGGGAATATGGAATCACCATTCCT
CCTGATCATAAGCCCAAATCCTGGGTTGCAGCAGAGAAAATGTACCACACTCATAGACGG
CGAAGGCTGGTCCGAAAACGCAAGAAAGATTTAACACAGACTGCTTCAAGCACCGCAAGG
GCCATGGAGGAATTGCAAGACCAAGAGGGCTGGGAATATGCTTCTCTAATTGGCTGGAAA
TTTCACTGGAAACAACGTAGTTCAGATACCTTCCGCCGCAGACGCTGGAGGAGAAAAATG
GCTCCTTCAGAAACACATGGTGCAGCTGCCATCTTTAAACTTGAAGGTGCCCTTGGGGCA
GACACTACCGAAGATGGGGATGAGAAGAGCCTGGAGAAACAGAAGCACAGTGCCACCACT
GTGTTCGGAGCAAACACCCCCATTGTTTCCTGCAATTTTGACAGAGTCTACATCTACCAT
CTGCGCTGCTATGTCTATCAAGCCAGAAACCTCTTGGCTTTAGATAAGGATAGCTTTTCA
GATCCATATGCTCATATCTGTTTCCTCCATCGGAGCAAAACCACTGAGATCATCCATTCA
ACCCTGAATCCCACGTGGGACCAAACAATTATATTCGATGAAGTTGAAATCTATGGGGAA
CCCCAAACAGTTCTACAGAATCCACCCAAAGTTATCATGGAACTTTTTGACAATGACCAA
GTGGGCAAAGATGAATTTGAAGCCAACAAAATCCTGCACCAGCACCTCGGGGCCCCTGAA
GAGCGGCTTGCTCTTCACATCCTCAGGACTCAGGGGCTGGTCCCTGAGCACGTGGAAACA
AGGACTTTGCACAGCACCTTCCAGCCCAACATTTCCCAGGGAAAACTTCAGATGTGGGTG
GATGTTTTCCCCAAGAGTTTGGGGCCACCAGGCCCTCCTTTCAACATCACACCCCGGAAA
GCCAAGAAATACTACCTGCGTGTGATCATCTGGAACACCAAGGACGTTATCTTGGACGAG
AAAAGCATCACAGGAGAGGAAATGAGTGACATCTACGTCAAAGGCTGGATTCCTGGCAAT
GAAGAAAACAAACAGAAAACAGATGTCCATTACAGATCTTTGGATGGTGAAGGGAATTTT
AACTGGCGATTTGTTTTCCCGTTTGACTACCTTCCAGCCGAACAACTCTGTATCGTTGCG
AAAAAAGAGCATTTCTGGAGTATTGACCAAACGGAATTTCGAATCCCACCCAGGCTGATC
ATTCAGATATGGGACAATGACAAGTTTTCTCTGGATGACTACTTGGGTTTCCTAGAACTT
GACTTGCGTCACACGATCATTCCTGCAAAATCACCAGAGAAATGCAGGTTGGACATGATT
CCGGACCTCAAAGCCATGAACCCCCTTAAAGCCAAGACAGCCTCCCTCTTTGAGCAGAAG
TCCATGAAAGGATGGTGGCCATGCTACGCAGAGAAAGATGGCGCCCGCGTAATGGCTGGG
AAAGTGGAGATGACATTGGAAATCCTCAACGAGAAGGAGGCCGACGAGAGGCCAGCCGGG
AAGGGGCGGGACGAACCCAACATGAACCCCAAGCTGGACTTACCAAATCGACCAGAAACC
TCCTTCCTCTGGTTCACCAACCCATGCAAGACCATGAAGTTCATCGTGTGGCGCCGCTTT
AAGTGGGTCATCATCGGCTTGCTGTTCCTGCTTATCCTGCTGCTCTTCGTGGCCGTGCTC
CTCTACTCTTTGCCGAACTATTTGTCAATGAAGATTGTAAAGCCAAATGTG
Protein sequence predicted based on nucleotide sequence
MLRVIVESASNIPKTKFGKPDPIVSVIFKDEKKKTKKVDNELNPVWNEILEFDLRGIPLD FSSSLGIIVKDFETIGQNKLIGTATVALKDLTGDQSRSLPYKLISLLNEKGQDTGATIDL VIGYDPPSAPHPNDLSGPSVPGMGGDGEEDEGDEDRLDNAVRGPGPKGPVGTVSEAQLAR RLTKVKNSRRMLSNKPQDFQIRVRVIEGRQLSGNNIRPVVKVHVCGQTHRTRIKRGNNPF FDELFFYNVNMTPSELMDEIISIRVYNSHSLRADCLMGEFKIDVGFVYDEPGHAVMRKWL LLNDPEDTSSGSKGYMKVSMFVLGTGDEPPPERRDRDNDSDDVESNLLLPAGIALRWVTF LLKIYRAEDIPQMDDAFSQTVKEIFGGNADKKNLVDPFVEVSFAGKKVCTNIIEKNANPE WNQVVNLQIKFPSVCEKIKLTIYDWDRLTKNDVVGTTYLHLSKIAASGGEVEDFSSSGTG AASYTVNTGETEVGFVPTFGPCYLNLYGSPREYTGFPDPYDELNTGKGEGVAYRGRILVE LATFLEKTPPDKKLEPISNDDLLVVEKYQRRRKYSLSAVFHSATMLQDVGEAIQFEVSIG NYGNKFDTTCKPLASTTQYSRAVFDGNYYYYLPWAHTKPVVTLTSYWEDISHRLDAVNTL LAMAERLQTNIEALKSGIQGKIPANQLAELWLKLIDEVIEDTRYTLPLTEGKANVTVLDT QIRKLRSRSLSQIHEAAVRMRSEATDVKSTLAEIEDWLDKLMQLTEEPQNSMPDIIIWMI RGEKRLAYARIPAHQVLYSTSGENASGKYCGKTQTIFLKYPQEKNNGPKVPVELRVNIWL GLSAVEKKFNSFAEGTFTVFAEMYENQALMFGKWGTSGLVGRHKFSDVTGKIKLKREFFL PPKGWEWEGEWIVDPERSLLTEADAGHTEFTDEVYQNESRYPGGDWKPAEDTYTDANGDK AASPSELTCPPGWEWEDDAWSYDINRAVDEKGWEYGITIPPDHKPKSWVAAEKMYHTHRR RRLVRKRKKDLTQTASSTARAMEELQDQEGWEYASLIGWKFHWKQRSSDTFRRRRWRRKM APSETHGAAAIFKLEGALGADTTEDGDEKSLEKQKHSATTVFGANTPIVSCNFDRVYIYH LRCYVYQARNLLALDKDSFSDPYAHICFLHRSKTTEIIHSTLNPTWDQTIIFDEVEIYGE PQTVLQNPPKVIMELFDNDQVGKDEFEANKILHQHLGAPEERLALHILRTQGLVPEHVET RTLHSTFQPNISQGKLQMWVDVFPKSLGPPGPPFNITPRKAKKYYLRVIIWNTKDVILDE KSITGEEMSDIYVKGWIPGNEENKQKTDVHYRSLDGEGNFNWRFVFPFDYLPAEQLCIVA KKEHFWSIDQTEFRIPPRLIIQIWDNDKFSLDDYLGFLELDLRHTIIPAKSPEKCRLDMI PDLKAMNPLKAKTASLFEQKSMKGWWPCYAEKDGARVMAGKVEMTLEILNEKEADERPAG KGRDEPNMNPKLDLPNRPETSFLWFTNPCKTMKFIVWRRFKWVIIGLLFLLILLLFVAVL LYSLPNYLSMKIVKPNV
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223
Synonyms:
pDONR223, pENTR223-XnCDSYn
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp):
2815
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Mark Vidal
Publications:
PMID:
15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
bacterial origin of replication
2220
2753
gene fragment
pas
5p stem of pBR primose assembly site
63
73
gene fragment
pas prime
3p stem of pBR pas(L)
105
116
recombination site
attL1
attL1 recombination site
570
665
recombination site
attL2
attL2 recombination site
793
696
selectable marker
SpnR
spectinomycin resistance gene
1062
2072
trxn termination sequence
T1-T2
rrnBT1 and T2 terminators
510
224
