DNASU Plasmid | PNPLA1 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: PNPLA1

Gene Name: patatin-like phospholipase domain containing 1

Original Clone ID:

ccsbBroadEn_09987

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC103905.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00516106

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1339nts

Open reading frame: 1 to 1339

Reference Sequence Alignment

ATGGTGCAGATGATGAGGCAGTTTCTGTACCGGGTCCTGCCCGAGGACTCCTACAAGGTC
ACCACGGGGAAGCTCCATGTGAGCCTCACCCGCTTAACGGACGGGGAGAATGTGGTGGTT
TCAGAGTTCACGTCCAAGGAGGAGCTCATTGAGGCCCTATACTGCAGCTGCTTCGTCCCG
GTGTACTGTGGCCTCATCCCCCCGACTTACCGCGGTGTGTGGGCATTTCTCACTCTGCCC
CCACAGAGGTACATCGATGGGGGCTTCACGGGCATGCAGCCCTGTGCCTTCTGGACCGAC
GCCATCACCATCTCCACCTTCAGTGGGCAGCAGGACATCTGTCCCCGGGACTGCCCGGCC
ATCTTCCACGACTTCCGCATGTTCAACTGCTCCTTCCAGTTCTCCCTGGAGAACATCGCC
AGGATGACCCACGCATTGTTCCCCCCGGACCTGGTGATCCTGCACGATTACTACTACCGA
GGGTACGAGGATGCAGTTTTGTACTTGAGGCGGCTGAATGCTGTTTATCTTAATTCTTCC
TCCAAGAGAGTGATTTTCCCCCGGGTGGAAGTGTACTGCCAGATAGAACTCGCCCTTGGC
AATGAGTGCCCTGAACGCAGTCAACCAAGCCTTCGAGCACGGCAGGCCAGTCTGGAAGGA
GCCACACAACCTCACAAGGAGTGGGTTCCCAAAGGGGATGGAAGGGGCAGCCATGGTCCG
CCTGTGTCCCAACCTGTGCAGACACTTGAATTCACATGCGAGTCACCTGTTTCAGCACCA
GTCTCTCCACTTGAGCAGCCACCTGCACAGCCACTGGCCTCTTCAACTCCACTTTCTCTA
AGTGGCATGCCACCTGTATCATTCCCAGCTGTGCACAAGCCACCCAGCTCCACACCTGGT
TCATCACTGCCCACCCCACCACCTGGACTGTCACCTCTGTCACCTCAGCAGCAGGTACAA
CCGTCTGGATCACCAGCCAGATCCCTACACTCTCAGGCACCCACTTCACACAGGCCATCC
CTGGGGCCTTCAACTGTGGGGGCACCTCAAACACTGCCCCGAAGTTCTCTTTCAGCCTTC
CCTGCTCAGCCACCTGTGGAGGAACTAGGCCAAGAACAGCCCCAAGCTGTAGCTCTTCTT
GTCTCTTCAAAACCAAAAAGCGCCGTGCCTCTGGTTCATGTGAAGGAAACCGTCAGCAAG
CCTTATGTAATGGAGAGCCCTGCTGAAGACTCAAACTGGGTGAATAAGGTCTTCAAGAAG
AACAAGCAAAAGACAAGTGGCACCAGAAAAGGCTTCCCAAGACATCCGGGATCCAAAAAA
CCAAGCAGCAAAGTGCAG

Protein sequence predicted based on nucleotide sequence

MVQMMRQFLYRVLPEDSYKVTTGKLHVSLTRLTDGENVVVSEFTSKEELIEALYCSCFVP
VYCGLIPPTYRGVWAFLTLPPQRYIDGGFTGMQPCAFWTDAITISTFSGQQDICPRDCPA
IFHDFRMFNCSFQFSLENIARMTHALFPPDLVILHDYYYRGYEDAVLYLRRLNAVYLNSS
SKRVIFPRVEVYCQIELALGNECPERSQPSLRARQASLEGATQPHKEWVPKGDGRGSHGP
PVSQPVQTLEFTCESPVSAPVSPLEQPPAQPLASSTPLSLSGMPPVSFPAVHKPPSSTPG
SSLPTPPPGLSPLSPQQQVQPSGSPARSLHSQAPTSHRPSLGPSTVGAPQTLPRSSLSAF
PAQPPVEELGQEQPQAVALLVSSKPKSAVPLVHVKETVSKPYVMESPAEDSNWVNKVFKK
NKQKTSGTRKGFPRHPGSKKPSSKVQ

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Protein

Clone

Organism

Genome
Reagents

Gene

Gene Symbol :

PNPLA1

Symbol Nomenclature :

PNPLA1

SYNONYM :

ARCI10; dJ50J22.1

Designation :

patatin-like phospholipase domain-containing protein 1

Full Nomenclature :

patatin-like phospholipase domain containing 1

GENEID :

285848

Locus Tag :

RP1-50J22.1

HGNC :

21246

MIM :

612121

Vega :

OTTHUMG00000014590

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00516106        ------------------------------------------------------------
NM_001145717.1      ATGGAAGAACAGGTGTTCAAGGGGGACCCGGACACCCCTCACTCCATCTCCTTCTCGGGC
NM_173676.2         ------------------------------------------------------------
NM_001145716.2      ------------------------------------------------------------
                                                                                

HsCD00516106        ------------------------------------------------------------
NM_001145717.1      AGTGGATTCCTCTCCTTCTACCAGGCGGGGGCTGTGGACGCCCTGCGGGACCTGGCCCCC
NM_173676.2         ------------------------------------------------------------
NM_001145716.2      ------------------------------------------------------------
                                                                                

HsCD00516106        ------------------------------------------------------------
NM_001145717.1      CGGATGCTGGAAACAGCCCACCGCTTTGCGGGGACATCGGCAGGTGCTGTGATCGCCGCC
NM_173676.2         ------------------------------------------------------------
NM_001145716.2      ------------------------------------------------------------
                                                                                

HsCD00516106        ------------------------------------------------------------
NM_001145717.1      CTGGCCATCTGCGGGATTGAAATGGATGAGTATCTCAGAGTCCTCAACGTGGGTGTGGCC
NM_173676.2         ------------------------------------------------------------
NM_001145716.2      ------------------------------------------------------------
                                                                                

HsCD00516106        ---------------------------------------------ATGGTGCAGATGATG
NM_001145717.1      GAGGTGAAGAAATCCTTCCTGGGGCCCTTGTCCCCGTCCTGTAAGATGGTGCAGATGATG
NM_173676.2         ---------------------------------------------ATGGTGCAGATGATG
NM_001145716.2      ---------------------------------------------ATGGTGCAGATGATG
                                                                 ***************

HsCD00516106        AGGCAGTTTCTGTACCGGGTCCTGCCCGAGGACTCCTACAAGGTCACCACGGGGAAGCTC
NM_001145717.1      AGGCAGTTTCTGTACCGGGTCCTGCCCGAGGACTCCTACAAGGTCACCACGGGGAAGCTC
NM_173676.2         AGGCAGTTTCTGTACCGGGTCCTGCCCGAGGACTCCTACAAGGTCACCACGGGGAAGCTC
NM_001145716.2      AGGCAGTTTCTGTACCGGGTCCTGCCCGAGGACTCCTACAAGGTCACCACGGGGAAGCTC
                    ************************************************************

HsCD00516106        CATGTGAGCCTCACCCGCTTAACGGACGGGGAGAATGTGGTGGTTTCAGAGTTCACGTCC
NM_001145717.1      CATGTGAGCCTCACCCGCTTAACGGACGGGGAGAATGTGGTGGTTTCAGAGTTCACGTCC
NM_173676.2         CATGTGAGCCTCACCCGCTTAACGGACGGGGAGAATGTGGTGGTTTCAGAGTTCACGTCC
NM_001145716.2      CATGTGAGCCTCACCCGCTTAACGGACGGGGAGAATGTGGTGGTTTCAGAGTTCACGTCC
                    ************************************************************

HsCD00516106        AAGGAGGAGCTCATTGAGGCCCTATACTGCAGCTGCTTCGTCCCGGTGTACTGTGGCCTC
NM_001145717.1      AAGGAGGAGCTCATTGAGGCCCTATACTGCAGCTGCTTCGTCCCGGTGTACTGTGGCCTC
NM_173676.2         AAGGAGGAGCTCATTGAGGCCCTATACTGCAGCTGCTTCGTCCCGGTGTACTGTGGCCTC
NM_001145716.2      AAGGAGGAGCTCATTGAGGCCCTATACTGCAGCTGCTTCGTCCCGGTGTACTGTGGCCTC
                    ************************************************************

HsCD00516106        ATCCCCCCGACTTACCGCG----GTGTGTGGGCATTTCTCACTCTGCCCCCACAGAGGTA
NM_001145717.1      ATCCCCCCGACTTACCGCGGTGTGAGG-------------------------------TA
NM_173676.2         ATCCCCCCGACTTACCGCG----GTGTGAGG---------------------------TA
NM_001145716.2      ATCCCCCCGACTTACCGCG----GTGTGTGGGCATTTCTCACTCTGCCCCCACAGAGGTA
                    *******************    *:*                                **

HsCD00516106        CATCGATGGGGGCTTCACGGGCATGCAGCCCTGTGCCTTCTGGACCGACGCCATCACCAT
NM_001145717.1      CATCGATGGGGGCTTCACGGGCATGCAGCCCTGTGCCTTCTGGACCGACGCCATCACCAT
NM_173676.2         CATCGATGGGGGCTTCACGGGCATGCAGCCCTGTGCCTTCTGGACCGACGCCATCACCAT
NM_001145716.2      CATCGATGGGGGCTTCACGGGCATGCAGCCCTGTGCCTTCTGGACCGACGCCATCACCAT
                    ************************************************************

HsCD00516106        CTCCACCTTCAGTGGGCAGCAGGACATCTGTCCCCGGGACTGCCCGGCCATCTTCCACGA
NM_001145717.1      CTCCACCTTCAGTGGGCAGCAGGACATCTGTCCCCGGGACTGCCCGGCCATCTTCCACGA
NM_173676.2         CTCCACCTTCAGTGGGCAGCAGGACATCTGTCCCCGGGACTGCCCGGCCATCTTCCACGA
NM_001145716.2      CTCCACCTTCAGTGGGCAGCAGGACATCTGTCCCCGGGACTGCCCGGCCATCTTCCACGA
                    ************************************************************

HsCD00516106        CTTCCGCATGTTCAACTGCTCCTTCCAGTTCTCCCTGGAGAACATCGCCAGGATGACCCA
NM_001145717.1      CTTCCGCATGTTCAACTGCTCCTTCCAGTTCTCCCTGGAGAACATCGCCAGGATGACCCA
NM_173676.2         CTTCCGCATGTTCAACTGCTCCTTCCAGTTCTCCCTGGAGAACATCGCCAGGATGACCCA
NM_001145716.2      CTTCCGCATGTTCAACTGCTCCTTCCAGTTCTCCCTGGAGAACATCGCCAGGATGACCCA
                    ************************************************************

HsCD00516106        CGCATTGTTCCCCCCGGACCTGGTGATCCTGCACGATTACTACTACCGAGGGTACGAGGA
NM_001145717.1      CGCATTGTTCCCCCCGGACCTGGTGATCCTGCACGATTACTACTACCGAGGGTACGAGGA
NM_173676.2         CGCATTGTTCCCCCCGGACCTGGTGATCCTGCACGATTACTACTACCGAGGGTACGAGGA
NM_001145716.2      CGCATTGTTCCCCCCGGACCTGGTGATCCTGCACGATTACTACTACCGAGGGTACGAGGA
                    ************************************************************

HsCD00516106        TGCAGTTTTGTACTTGAGGCGGCTGAATGCTGTTTATCTTAATTCTTCCTCCAAGAGAGT
NM_001145717.1      TGCAGTTTTGTACTTGAGGCGGCTGAATGCTGTTTATCTTAATTCTTCCTCCAAGAGAGT
NM_173676.2         TGCAGTTTTGTACTTGAGGCGGCTGAATGCTGTTTATCTTAATTCTTCCTCCAAGAGAGT
NM_001145716.2      TGCAGTTTTGTACTTGAGGCGGCTGAATGCTGTTTATCTTAATTCTTCCTCCAAGAGAGT
                    ************************************************************

HsCD00516106        GATTTTCCCCCGGGTGGAAGTGTACTGCCAGATAGAACTCGCCCTTGGCAATGAGTGCCC
NM_001145717.1      GATTTTCCCCCGGGTGGAAGTGTACTGCCAGATAGAACTCGCCCTTGGCAATGAGTGCCC
NM_173676.2         GATTTTCCCCCGGGTGGAAGTGTACTGCCAGATAGAACTCGCCCTTGGCAATGAGTGCCC
NM_001145716.2      GATTTTCCCCCGGGTGGAAGTGTACTGCCAGATAGAACTCGCCCTTGGCAATGAGTGCCC
                    ************************************************************

HsCD00516106        TGAACGCAGTCAACCAAGCCTTCGAGCACGGCAGGCCAGTCTGGAAGGAGCCACACAACC
NM_001145717.1      TGAACGCAGTCAACCAAGCCTTCGAGCACGGCAGGCCAGTCTGGAAGGAGCCACACAACC
NM_173676.2         TGAACGCAGTCAACCAAGCCTTCGAGCACGGCAGGCCAGTCTGGAAGGAGCCACACAACC
NM_001145716.2      TGAACGCAGTCAACCAAGCCTTCGAGCACGGCAGGCCAGTCTGGAAGGAGCCACACAACC
                    ************************************************************

HsCD00516106        TCACAAGGAGTGGGTTCCCAAAGGGGATGGAAGGGGCAGCCATGGTCCGCCTGTGTCCCA
NM_001145717.1      TCACAAGGAGTGGGTTCCCAAAGGGGATGGAAGGGGCAGCCATGGTCCGCCTGTGTCCCA
NM_173676.2         TCACAAGGAGTGGGTTCCCAAAGGGGATGGAAGGGGCAGCCATGGTCCGCCTGTGTCCCA
NM_001145716.2      TCACAAGGAGTGGGTTCCCAAAGGGGATGGAAGGGGCAGCCATGGTCCGCCTGTGTCCCA
                    ************************************************************

HsCD00516106        ACCTGTGCAGACACTTGAATTCACATGCGAGTCACCTGTTTCAGCACCAGTCTCTCCACT
NM_001145717.1      ACCTGTGCAGACACTTGAATTCACATGCGAGTCACCTGTTTCAGCACCAGTCTCTCCACT
NM_173676.2         ACCTGTGCAGACACTTGAATTCACATGCGAGTCACCTGTTTCAGCACCAGTCTCTCCACT
NM_001145716.2      ACCTGTGCAGACACTTGAATTCACATGCGAGTCACCTGTTTCAGCACCAGTCTCTCCACT
                    ************************************************************

HsCD00516106        TGAGCAGCCACCTGCACAGCCACTGGCCTCTTCAACTCCACTTTCTCTAAGTGGCATGCC
NM_001145717.1      TGAGCAGCCACCTGCACAGCCACTGGCCTCTTCAACTCCACTTTCTCTAAGTGGCATGCC
NM_173676.2         TGAGCAGCCACCTGCACAGCCACTGGCCTCTTCAACTCCACTTTCTCTAAGTGGCATGCC
NM_001145716.2      TGAGCAGCCACCTGCACAGCCACTGGCCTCTTCAACTCCACTTTCTCTAAGTGGCATGCC
                    ************************************************************

HsCD00516106        ACCTGTATCATTCCCAGCTGTGCACAAGCCACCCAGCTCCACACCTGGTTCATCACTGCC
NM_001145717.1      ACCTGTATCATTCCCAGCTGTGCACAAGCCACCCAGCTCCACACCTGGTTCATCACTGCC
NM_173676.2         ACCTGTATCATTCCCAGCTGTGCACAAGCCACCCAGCTCCACACCTGGTTCATCACTGCC
NM_001145716.2      ACCTGTATCATTCCCAGCTGTGCACAAGCCACCCAGCTCCACACCTGGTTCATCACTGCC
                    ************************************************************

HsCD00516106        CACCCCACCACCTGGACTGTCACCTCTGTCACCTCAGCAGCAGGTACAACCGTCTGGATC
NM_001145717.1      CACCCCACCACCTGGACTGTCACCTCTGTCACCTCAGCAGCAGGTACAACCGTCTGGATC
NM_173676.2         CACCCCACCACCTGGACTGTCACCTCTGTCACCTCAGCAGCAGGTACAACCGTCTGGATC
NM_001145716.2      CACCCCACCACCTGGACTGTCACCTCTGTCACCTCAGCAGCAGGTACAACCGTCTGGATC
                    ************************************************************

HsCD00516106        ACCAGCCAGATCCCTACACTCTCAGGCACCCACTTCACACAGGCCATCCCTGGGGCCTTC
NM_001145717.1      ACCAGCCAGATCCCTACACTCTCAGGCACCCACTTCACCCAGGCCATCCCTGGGGCCTTC
NM_173676.2         ACCAGCCAGATCCCTACACTCTCAGGCACCCACTTCACCCAGGCCATCCCTGGGGCCTTC
NM_001145716.2      ACCAGCCAGATCCCTACACTCTCAGGCACCCACTTCACCCAGGCCATCCCTGGGGCCTTC
                    **************************************.*********************

HsCD00516106        AACTGTGGGGGCACCTCAAACACTGCCCCGAAGTTCTCTTTCAGCCTTCCCTGCTCAGCC
NM_001145717.1      AACTGTGGGGGCACCTCAAACACTGCCCCGAAGTTCTCTTTCAGCCTTCCCTGCTCAGCC
NM_173676.2         AACTGTGGGGGCACCTCAAACACTGCCCCGAAGTTCTCTTTCAGCCTTCCCTGCTCAGCC
NM_001145716.2      AACTGTGGGGGCACCTCAAACACTGCCCCGAAGTTCTCTTTCAGCCTTCCCTGCTCAGCC
                    ************************************************************

HsCD00516106        ACCTGTGGAGGAACTAGGCCAAGAACAGCCCCAAGCTGTAGCTCTTCTTGTCTCTTCAAA
NM_001145717.1      ACCTGTGGAGGAACTAGGCCAAGAACAGCCCCAAGCTGTAGCTCTTCTTGTCTCTTCAAA
NM_173676.2         ACCTGTGGAGGAACTAGGCCAAGAACAGCCCCAAGCTGTAGCTCTTCTTGTCTCTTCAAA
NM_001145716.2      ACCTGTGGAGGAACTAGGCCAAGAACAGCCCCAAGCTGTAGCTCTTCTTGTCTCTTCAAA
                    ************************************************************

HsCD00516106        ACCAAAAAGCGCCGTGCCTCTGGTTCATGTGAAGGAAACCGTCAGCAAGCCTTATGTAAT
NM_001145717.1      ACCAAAAAGCGCCGTGCCTCTGGTTCATGTGAAGGAAACCGTCAGCAAGCCTTATGTAAC
NM_173676.2         ACCAAAAAGCGCCGTGCCTCTGGTTCATGTGAAGGAAACCGTCAGCAAGCCTTATGTAAC
NM_001145716.2      ACCAAAAAGCGCCGTGCCTCTGGTTCATGTGAAGGAAACCGTCAGCAAGCCTTATGTAAC
                    *********************************************************** 

HsCD00516106        GGAGAGCCCTGCTGAAGACTCAAACTGGGTGAATAAGGTCTTCAAGAAGAACAAGCAAAA
NM_001145717.1      GGAGAGCCCTGCTGAAGACTCAAACTGGGTGAATAAGGTCTTCAAGAAGAACAAGCAAAA
NM_173676.2         GGAGAGCCCTGCTGAAGACTCAAACTGGGTGAATAAGGTCTTCAAGAAGAACAAGCAAAA
NM_001145716.2      GGAGAGCCCTGCTGAAGACTCAAACTGGGTGAATAAGGTCTTCAAGAAGAACAAGCAAAA
                    ************************************************************

HsCD00516106        GACAAGTGGCACCAGAAAAGGCTTCCCAAGACATCCGGGATCCAAAAAACCAAGCAGCAA
NM_001145717.1      GACAAGTGGCACCAGAAAAGGCTTCCCAAGACATTCGGGATCCAAAAAACCAAGCAGCAA
NM_173676.2         GACAAGTGGCACCAGAAAAGGCTTCCCAAGACATTCGGGATCCAAAAAACCAAGCAGCAA
NM_001145716.2      GACAAGTGGCACCAGAAAAGGCTTCCCAAGACATTCGGGATCCAAAAAACCAAGCAGCAA
                    ********************************** *************************

HsCD00516106        AGTGCAG---
NM_001145717.1      AGTGCAGTGA
NM_173676.2         AGTGCAGTGA
NM_001145716.2      AGTGCAGTGA
                    *******