Gene:
Gene Symbol:
EPC2
Gene Name:
enhancer of polycomb homolog 2 (Drosophila)
Original Clone ID:
ccsbBroadEn_11813
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00516272
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 1nts
Open reading frame: 69 to 2418
GTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAG
TTGGCACCATGCCTGATCTCAACGACTGCGTCTCCATCAACCGGGCCGTGCCCCAGATGC
CCACCGGGATGGAGAAGGAGGAGGAATCGGAACATCATTTACAGCGAGCAATTTCAGCAC
AGCAAGTGTTTAGAGAAAAAAAAGAGAGTATGGTCATTCCTGTTCCTGAGGCAGAGAGCA
ACGTCAACTATTACAATCGCTTGTACAAAGGAGAGTTTAAACAGCCAAAACAGTTCATTC
ATATTCAGCCTTTTAATCTAGACAACGAGCAACCAGATTATGATATGGATTCAGAAGATG
AGACTTTATTAAATAGACTTAACAGAAAGATGGAAATTAAGCCTTTGCAATTTGAAATTA
TGATTGACAGACTTGAAAAAGCCAGTTCTAATCAGCTTGTAACACTTCAAGAAGCAAAAC
TGCTGCTAAACGAAGATGATTACCTTATTAAAGCTGTATATGACTACTGGGTGAGAAAAC
GTAAAAACTGCAGGGGGCCATCCCTCATTCCTCAGATAAAACAAGAGAAAAGAGATGGCT
CTACCAACAATGACCCTTATGTTGCCTTTCGGAGAAGAACAGAGAAAATGCAAACTCGAA
AGAATCGTAAGAATGATGAAGCCTCTTATGAAAAGATGTTGAAACTGAGACGAGAATTTA
GTAGAGCCATAACAATTTTGGAAATGATTAAGAGAAGAGAGAAAACAAAACGAGAATTAT
TGCACTTAACCTTAGAAGTTGTGGAGAAAAGATACCATTTGGGAGACTATGGTGGTGAAA
TCCTTAATGAAGTAAAAATCAGTAGATCAGAAAAAGAGTTATATGCCACTCCAGCAACTC
TTCATAATGGAAATCATCACAAAGTTCAAGAATGTAAAACTAAGCACCCTCATCATTTGT
CTTTGAAAGAAGAGGCTTCTGATGTGGTTCGTCAAAAGAAGAAGTACCCAAAGAAGCCTA
AAGCAGAGGCTTTGATAACATCTCAGCAACCCACTCCTGAGACATTGCCTGTGATCAATA
AGAGTGACATTAAGCAATATGATTTTCACAGCTCAGATGAAGATGAATTTCCACAGGTAT
TGTCCCCAGTATCAGAACCGGAAGAAGAAAATGATCCTGATGGTCCCTGTGCTTTCAGAA
GGCGGGCAGGATGCCAGTATTATGCTCCTCGTTTGGACCAAGCTAACCATTCATGTGAAA
ATTCAGAATTGGCAGATTTGGATAAGTTGAGGTATAGGCATTGCCTTACAACACTTACAG
TCCCAAGAAGATGTATAGGATTTGCAAGGAGGCGAATTGGCAGAGGTGGAAGGGTCATAA
TGGACCGAATATCCACAGAACATGACCCAGTCCTGAAACAGATAGACCCTGAAATGCTGA
ATAGTTTTTCAAGCTCTTCCCAAACTATAGACTTTTCTTCTAATTTCTCTCGGACCAATG
CTTCCAGTAAACATTGTGAAAATAGACTGTCTCTTTCTGAAATATTAAGCAATATCAGAT
CATGTCGACTACAGTGTTTCCAGCCAAGGCTACTAAATTTACAGGACAGTGATAGTGAAG
AATGTACCTCAAGAAAACCAGGGCAGACTGTGAACAATAAAAGAGTTTCTGCAGCATCTG
TAGCTTTATTGAACACCAGCAAGAATGGCATATCAGTAACAGGGGGTATCACAGAAGAGC
AGTTTCAGACACATCAGCAGCAGTTAGTTCAGATGCAAAGGCAGCAACTTGCCCAGCTTC
AGCAGAAACAGCAATCTCAGCATTCCTCGCAACAGACACATCCAAAAGCACAGGGCTCAA
GCACCTCTGACTGTATGTCTAAAACACTTGACTCAGCCAGCGCCCACTTTGCTGCATCTG
CAGTGGTCAGTGCACCTGTTCCAAGTCGCAGTGAGGTAGCCAAGGAACAGAACACTGGCC
ACAACAACATAAACGGTGTTGTCCAGCCTTCAGGAACCTCTAAAACATTATACTCCACCA
ATATGGCTTTATCATCCAGCCCAGGGATTTCAGCTGTACAGCTTGTAAGGACAGTTGGCC
ACACCACTACAAACCACTTAATCCCAGCATTGTGCACAAGCAGTCCTCAGACACTTCCCA
TGAACAATTCCTGCCTGACAAATGCAGTGCACCTCAATAATGTCAGTGTTGTTTCTCCAG
TCAATGTGCATATCAATACACGGACTTCAGCACCATCGCCAACAGCCTTAAAACTTGCCA
CAGTTGCTGCCAGTATGGACAGAGTGCCAAAGGTTACTCCCAGCAGTGCCATCAGCAGCA
TAGCAAGAGAGAACCACGAACCAGAAAGATTGGGCTTAAATGGAATAGCAGAGACAACAG
TAGCTATGGAAGTGACATTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATT
GCTTATCAATTTGTTGCAACGAAC
Protein sequence predicted based on nucleotide sequence
MPDLNDCVSINRAVPQMPTGMEKEEESEHHLQRAISAQQVFREKKESMVIPVPEAESNVN YYNRLYKGEFKQPKQFIHIQPFNLDNEQPDYDMDSEDETLLNRLNRKMEIKPLQFEIMID RLEKASSNQLVTLQEAKLLLNEDDYLIKAVYDYWVRKRKNCRGPSLIPQIKQEKRDGSTN NDPYVAFRRRTEKMQTRKNRKNDEASYEKMLKLRREFSRAITILEMIKRREKTKRELLHL TLEVVEKRYHLGDYGGEILNEVKISRSEKELYATPATLHNGNHHKVQECKTKHPHHLSLK EEASDVVRQKKKYPKKPKAEALITSQQPTPETLPVINKSDIKQYDFHSSDEDEFPQVLSP VSEPEEENDPDGPCAFRRRAGCQYYAPRLDQANHSCENSELADLDKLRYRHCLTTLTVPR RCIGFARRRIGRGGRVIMDRISTEHDPVLKQIDPEMLNSFSSSSQTIDFSSNFSRTNASS KHCENRLSLSEILSNIRSCRLQCFQPRLLNLQDSDSEECTSRKPGQTVNNKRVSAASVAL LNTSKNGISVTGGITEEQFQTHQQQLVQMQRQQLAQLQQKQQSQHSSQQTHPKAQGSSTS DCMSKTLDSASAHFAASAVVSAPVPSRSEVAKEQNTGHNNINGVVQPSGTSKTLYSTNMA LSSSPGISAVQLVRTVGHTTTNHLIPALCTSSPQTLPMNNSCLTNAVHLNNVSVVSPVNV HINTRTSAPSPTALKLATVAASMDRVPKVTPSSAISSIARENHEPERLGLNGIAETTVAM EVT
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223
Synonyms:
pDONR223, pENTR223-XnCDSYn
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp):
2815
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Mark Vidal
Publications:
PMID:
15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
bacterial origin of replication
2220
2753
gene fragment
pas
5p stem of pBR primose assembly site
63
73
gene fragment
pas prime
3p stem of pBR pas(L)
105
116
recombination site
attL1
attL1 recombination site
570
665
recombination site
attL2
attL2 recombination site
793
696
selectable marker
SpnR
spectinomycin resistance gene
1062
2072
trxn termination sequence
T1-T2
rrnBT1 and T2 terminators
510
224
