Gene:
Gene Symbol:
PPFIBP1
Gene Name:
PTPRF interacting protein, binding protein 1 (liprin beta 1)
Original Clone ID:
ccsbBroadEn_07246
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC050281.1
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00516398
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGATGAGTGATGCAAGTGACATGTTGGCTGCAGCGTTGGAGCAGATGGATGGTATCATA
GCAGGTTCTAAGGCTCTGGAATATTCCAATGGGATTTTTGATTGCCAATCTCCCACCTCT
CCATTCATGGGAAGTTTGCGAGCTCTGCACCTTGTGGAAGACCTGCGTGGATTGTTAGAG
ATGATGGAAACAGATGAGAAAGAAGGCTTGAGATGCCAGATCCCAGATTCAACAGCAGAA
ACGCTTGTTGAGTGGCTTCAGAGTCAAATGACAAATGGACACCTACCAGGGAACGGAGAT
GTGTATCAAGAAAGGCTGGCACGTTTAGAAAATGATAAAGAATCCCTCGTTCTTCAGGTA
AGTGTGTTAACAGACCAGGTGGAGGCTCAGGGAGAGAAGATTCGAGATTTGGAGTTTTGT
CTTGAAGAGCACAGAGAGAAGTTGAATGCCACAGAAGAAATGCTGCAGCAGGAGCTTCTA
AGTAGGACATCCTTAGAAACTCAGAAGTTGGATCTGATGGCTGAAATATCTAACTTGAAG
TTGAAACTGACAGCTGTAGAGAAGGACAGATTGGATTATGAAGATAAGTTCAGAGACACA
GAGGGGCTGATTCAGGAGATCAATGATTTGAGGTTAAAAGTTAGTGAAATGGACAGTGAG
AGACTTCAGTATGAAAAAAAGCTTAAATCAACCAAATCTTTAATGGCCAAACTTTCTAGC
ATGAAAATCAAGGTGGGTCAGATGCAGTATGAAAAGCAGCGGATGGAACAAAAATGGGAG
TCACTGAAGGATGAACTGGCATCTTTAAAAGAACAACTAGAAGAAAAGGAATCTGAAGTA
AAAAGGCTACAAGAAAAATTGGTTTGCAAGATGAAAGGAGAAGGGGTTGAAATTGTTGAT
AGAGACATCGAAGTACAAAAAATGAAAAAAGCTGTGGAGTCCTTGATGGCAGCAAATGAA
GAAAAGGATCGGAAAATAGAAGATCTTCGACAGTGCCTGAACAGGTACAAGAAAATGCAA
GACACGGTGGTACTGGCCCAAGGCAAAGATGGAGAATATGAAGAGCTGCTCAATTCCAGT
TCCATCTCCTCTTTGCTGGATGCACAGGGTTTCAGTGATCTGGAGAAAAGTCCATCACCC
ACTCCAGTAATGGGATCTCCCAGTTGTGACCCATTTAACACAAGTGTTCCCGAAGAGTTC
CATACTACCATCTTGCAAGTTTCCATCCCTTCATTATTGCCAGCAACTGTAAGCATGGAA
ACTTCTGAAAAATCAAAGTTGACTCCTAAGCCAGAGACTTCATTTGAAGAAAATGATGGA
AACATAATCCTTGGTGCCACTGTTGATACCCAACTGTGTGATAAACTTTTAACTTCAAGT
CTGCAGAAGTCCAGCAGCCTGGGCAATCTGAAGAAAGAGACATCTGATGGGGAAAAGGAA
ACTATTCAGAAGACTTCAGAGGACAGAGCTCCGGCAGAAAGCAGGCCATTTGGGACCCTT
CCTCCCAGGCCCCCAGGGCAGGACACCTCCATGGATGACAACCCCTTCGGCACTCGAAAA
GTCAGATCTTCCTTTGGCCGGGGCTTTTTTAAAATCAAAAGTAACAAGAGAACAGCAAGT
GCACCAAACTTAGCTGAAACAGAAAAAGAGACAGCAGAGCACCTAGATCTGGCTGGTGCT
TCTTCTCGGCCAAAAGATTCACAGAGGAACAGTCCCTTCCAGATACCGCCTCCATCTCCA
GATTCCAAAAAGAAATCCAGAGGTATCATGAAACTCTTTGGAAAACTTAGGAGAAGTCAA
TCAACTACATTCAACCCAGATGACATGTCTGAGCCTGAATTCAAAAGAGGAGGGACAAGG
GCAACCGCGGGGCCCCGATTAGGTTGGTCTCGAGACTTGGGACAGTCTAACAGTGACTTG
GATATGCCATTTGCCAAGTGGACCAAGGAGCAGGTTTGCAATTGGCTGATGGAACAGGGC
TTGGGCTCGTACCTGAATTCTGGCAAGCACTGGATTGCATCTGGCCAAACGCTTTTGCAG
GCTTCTCAACAAGATCTAGAGAAGGAACTTGGAATCAAGCATTCACTTCATCGAAAGAAA
CTCCAGCTAGCACTCCAAGCCCTGGGATCTGAAGAAGAAACCAATCATGGGAAGCTGGAT
TTCAACTGGGTCACTAGATGGTTGGATGACATTGGCCTCCCTCAATATAAGACCCAGTTT
GATGAAGGACGGGTTGATGGTCGAATGCTACATTACATGACTGTTGATGACTTACTGTCT
CTGAAGGTTGTAAGTGTGCTACACCATCTCAGTATCAAAAGGGCCATCCAGGTCCTGAGG
ATCAATAACTTTGAACCAAACTGTCTACGGAGGCGGCCATCTGATGAGAATACCATCGCC
CCATCAGAAGTTCAGAAGTGGACTAACCATCGAGTGATGGAGTGGCTGCGCTCCGTGGAC
TTGGCAGAATATGCGCCCAATCTCAGAGGCAGTGGTGTCCATGGTGGGCTCATGGTTCTA
GAGCCTCGTTTTAACGTAGAAACAATGGCTCAGTTATTGAACATCCCACCCAATAAGACT
TTGCTGCGAAGACATTTGGCCACTCATTTCAACCTTCTGATTGGGGCTGAGGCACAGCAC
CAGAAGCGAGATGCCATGGAGCTGCCGGATTATGTACTTCTAACAGCTACTGCCAAAGTG
AAGCCAAAGAAACTTGCCTTTAGCAATTTTGGGAATTTGAGAAAGAAGAAACAGGAAGAT
GGTGAAGAATATGTTTGTCCAATGGAATTGGGACAGGCATCAGGAAGTGCATCTAAGAAA
GGATTTAAACCTGGTTTGGATATGCGCCTGTATGAGGAAGATGATTTGGACCGGTTAGAG
CAGATGGAAGATTCAGAAGGGACAGTGAGACAGATAGGTGCATTCTCTGAAGGCATCAAC
AATCTGACGCACATGTTAAAAGAAGATGACATGTTTAAAGATTTTGCTGCCCGTTCCCCC
AGTGCCAGCATTACAGATGAAGACTCAAACGTT
Protein sequence predicted based on nucleotide sequence
MMSDASDMLAAALEQMDGIIAGSKALEYSNGIFDCQSPTSPFMGSLRALHLVEDLRGLLE MMETDEKEGLRCQIPDSTAETLVEWLQSQMTNGHLPGNGDVYQERLARLENDKESLVLQV SVLTDQVEAQGEKIRDLEFCLEEHREKLNATEEMLQQELLSRTSLETQKLDLMAEISNLK LKLTAVEKDRLDYEDKFRDTEGLIQEINDLRLKVSEMDSERLQYEKKLKSTKSLMAKLSS MKIKVGQMQYEKQRMEQKWESLKDELASLKEQLEEKESEVKRLQEKLVCKMKGEGVEIVD RDIEVQKMKKAVESLMAANEEKDRKIEDLRQCLNRYKKMQDTVVLAQGKDGEYEELLNSS SISSLLDAQGFSDLEKSPSPTPVMGSPSCDPFNTSVPEEFHTTILQVSIPSLLPATVSME TSEKSKLTPKPETSFEENDGNIILGATVDTQLCDKLLTSSLQKSSSLGNLKKETSDGEKE TIQKTSEDRAPAESRPFGTLPPRPPGQDTSMDDNPFGTRKVRSSFGRGFFKIKSNKRTAS APNLAETEKETAEHLDLAGASSRPKDSQRNSPFQIPPPSPDSKKKSRGIMKLFGKLRRSQ STTFNPDDMSEPEFKRGGTRATAGPRLGWSRDLGQSNSDLDMPFAKWTKEQVCNWLMEQG LGSYLNSGKHWIASGQTLLQASQQDLEKELGIKHSLHRKKLQLALQALGSEEETNHGKLD FNWVTRWLDDIGLPQYKTQFDEGRVDGRMLHYMTVDDLLSLKVVSVLHHLSIKRAIQVLR INNFEPNCLRRRPSDENTIAPSEVQKWTNHRVMEWLRSVDLAEYAPNLRGSGVHGGLMVL EPRFNVETMAQLLNIPPNKTLLRRHLATHFNLLIGAEAQHQKRDAMELPDYVLLTATAKV KPKKLAFSNFGNLRKKKQEDGEEYVCPMELGQASGSASKKGFKPGLDMRLYEEDDLDRLE QMEDSEGTVRQIGAFSEGINNLTHMLKEDDMFKDFAARSPSASITDEDSNV
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223
Synonyms:
pDONR223, pENTR223-XnCDSYn
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp):
2815
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Mark Vidal
Publications:
PMID:
15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
bacterial origin of replication
2220
2753
gene fragment
pas
5p stem of pBR primose assembly site
63
73
gene fragment
pas prime
3p stem of pBR pas(L)
105
116
recombination site
attL1
attL1 recombination site
570
665
recombination site
attL2
attL2 recombination site
793
696
selectable marker
SpnR
spectinomycin resistance gene
1062
2072
trxn termination sequence
T1-T2
rrnBT1 and T2 terminators
510
224
