Gene:
Gene Symbol:
TUBGCP5
Gene Name:
tubulin, gamma complex associated protein 5
Original Clone ID:
ccsbBroadEn_09399
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC071560.1
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00516403
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGCGCGGCACGGGCCACCGTGGAGTCGGTTGGACGCGCAGCAGGAGCGCGACGTGCGG
GAGCTCGTCCGGGGTGTCGCCGGCCTCCAGGACGAGGCAGACCCCAACTTCCAGCTCGCC
CTAAACTTCGCCTGGTCCAACTTCAGATTTCATCGTTTCTTGGATGTCAACAGCCACAAA
ATAGAAAAAACAATCGAAGGAATTTATGAAAAATTTGTCATTCATTCTGATCTAAGCAAA
GCTGCTAGTTGGAAGAGATTAACGGAGGAATTTCTAAATGCACCACTTCCCAGTATAAAG
GAAATAAAGACAGATGCACATTATTCCATACTGTCACTTCTTCTGTGTCTGTCAGACTCT
CCTTCAAACAGCAGTTATGTGGAGACACCAAGAAATAAAGAAGTGGAAAAGAAAGATGAT
TTCGACTGGGGAAAATACTTGATGGAAGATGAAGAAATGGACATTGGTCCGTACATGGAC
ACACCAAATTGGTCTGAAGAAAGTGAAGAGGAAAATGATCAACAGCCCTTAAGCAGAGAG
GACTCTGGAATTCAGGTAGACAGGACACCGTTAGAAGAACAAGATCAAAACGGAAAACTG
GATCCTTGTATCAGTTGGAAAGATGAGCCAGATGACCGAAGCTGGCTGGAACATCATGTG
GTCCATCAGTACTGGACAGCCAGGCCCTCCCAGTTTCCTCATAGTTTACATTTGCACTCT
AATTTAGCTGCTGTCTGGGACCAACACTTGTACAGCAGTGATCCATTGTATGTTCCAGAT
GACAGGGTTTTGGTTACTGAGACTCAGGTTATTCGGGAAACCCTATGGTTACTTTCAGGA
GTGAAAAAGCTCTTTATATTTCAGTTGATAGATGGGAAGGTAACTGTGAGAAACAATATT
ATAGTAACTCATTTAACACATAGCTGTTTACGATCTGTGCTGGAACAAATAGCAGCATAT
GGCCAGGTTGTGTTTCGACTCCAGGAGTTCATTGATGAAGTCATGGGACACAGTTCTGAG
AGCATGCTGCCTGGAAGTGGGTCTGTTCCTAAGAAGTCAACTGAAGCTCCCTTTAGAACC
TACCAGGCTTTCATGTGGGCCCTGTACAAATATTTCATTAGTTTCAAAGAGGAACTTGCA
GAAATTGAGAAGTGCATCATCAATAATGATACTACAATAACTCTTGCAATAGTGGTGGAC
AAGTTGGCACCTCGATTGTCTCAGCTCAAGGTTCTGCACAAAGTGTTTAGTACTGGAGTA
GCAGAAGTTCCACCTGATACTCGAAATGTCGTCCGGGCCTCTCACCTGCTTAACACCCTG
TACAAGGCCATTCTTGAATATGACAATGTTGGAGAAGCCTCTGAGCAAACCGTCTCCCTC
CTTTTCTCTCTCTGGGTGGAAACGGTGCGGCCTTACCTGCAGACGGTGGACGAGTGGATC
GTGCACGGGCACCTGTGGGATGGCGCCAGGGAGTTCATCATCCAGAGAAACAAAAATGTT
CCAGTTAATCACAGAGACTTCTGGTATGCAACTTACACGTTATATAGCGTATCAGAAAAG
ACAGAAAATGAAGAAAAAATGAGTGATAACGCTAGTGCGAGTTCCGGCAGTGACCAGGGG
CCCTCCAGCAGGCAGCACACCATGGTGTCCTTCCTCAAACCTGTCCTGAAGCAGATCATA
ATGGCTGGCAAGTCGATGCAGCTGCTGAAGAACCTGCAGTGTGCGGAGAGCACCACCTGC
CAGGCAGGAGCCAGAGATGCAGAAAGAAAAAGTTTATACACTCTCTTTCTGGAATCTGTA
CAGTCCCGTCTTCGACATGGAGAAGATTCCACTCCACAGGTTCTTACTGAGCAACAGGCA
ACCAAGGAGAACCTGATGAAGATGCAGTCCATTGCTGAAAGCCATCTTGAACTGGATGAT
GTTCATGATCCACTGCTTGCCATTAACTTTGCAAGGATGTATTTGGAGCAGAGTGACTTT
CACGAGAAGTTTGCTGGTGGTGATGTATGTGTGGATAGATCATCGGAATCTGTGACATGC
CAGACTTTTGAATTAACGCTGAGATCCTGCCTCTATCCTCATATTGACAAGCAGTATCTA
GATTGCTGTGGAAATCTCATGCAAACTCTAAAAAAAGATTACAGGTTGGTAGAATACTTG
CAAGCTATGAGGAATTTTTTCTTAATGGAAGGAGGAGATACCATGTATGACTTCTACACG
TCAATTTTTGATAAAATAAGAGAAAAGGAAACATGGCAGAATGTGTCTTTTCTTAATGTC
CAACTCCAAGAAGCAGTAGGACAGCGTTATCCTGAAGATAGTTCACGTCTATCTATATCT
TTTGAAAATGTTGACACAGCTAAGAAGAAGCTGCCTGTTCATATCTTAGATGGTCTGACC
CTCAGCTACAAGGTCCCATGGCCCGTGGACATTGTTATAAGTTTGGAATGTCAAAAAATT
TATAATCAAGTGTTTCTTCTCTTATTGCAAATAAAGTGGGCAAAATATAGTCTGGATGTT
TTACTTTTTGGTGAACTGGTTAGTACTGCAGAAAAACCACGACTTAAAGAAGGCCTTATA
CATGAACAAGACACAGTTGCTCAGTTCGGACCACAAAAAGAACCAGTAAGACAGCAGATT
CATCGCATGTTCCTCTTAAGAGTGAAGCTCATGCATTTCGTGAACAGCTTGCACAACTAC
ATCATGACCAGGATTCTACACAGTACAGGGCTGGAGTTTCAACATCAAGTCGAGGAAGCC
AAGGATTTAGATCAATTGATTAAAATTCACTATAGGTATCTGTCAACCATCCATGACCGG
TGTCTGCTGAGAGAAAAGGTCAGCTTTGTGAAAGAAGCTATCATGAAGGTGTTGAACTTG
GCTCTCATGTTTGCAGACGGTTGGCAGGCAGGCCTGGGCACTTGGCGAATGGAATCTATA
GAGAAAATGGAATCTGATTTTAAAAACTGCCATATGTTTCTTGTAACCATTTTAAACAAA
GCCGTCTGTAGAGGATCCTTTCCCCATTTGGAATCTCTAGCGTTGTCACTCATGGCTGGC
ATGGAACAAAGT
Protein sequence predicted based on nucleotide sequence
MARHGPPWSRLDAQQERDVRELVRGVAGLQDEADPNFQLALNFAWSNFRFHRFLDVNSHK IEKTIEGIYEKFVIHSDLSKAASWKRLTEEFLNAPLPSIKEIKTDAHYSILSLLLCLSDS PSNSSYVETPRNKEVEKKDDFDWGKYLMEDEEMDIGPYMDTPNWSEESEEENDQQPLSRE DSGIQVDRTPLEEQDQNGKLDPCISWKDEPDDRSWLEHHVVHQYWTARPSQFPHSLHLHS NLAAVWDQHLYSSDPLYVPDDRVLVTETQVIRETLWLLSGVKKLFIFQLIDGKVTVRNNI IVTHLTHSCLRSVLEQIAAYGQVVFRLQEFIDEVMGHSSESMLPGSGSVPKKSTEAPFRT YQAFMWALYKYFISFKEELAEIEKCIINNDTTITLAIVVDKLAPRLSQLKVLHKVFSTGV AEVPPDTRNVVRASHLLNTLYKAILEYDNVGEASEQTVSLLFSLWVETVRPYLQTVDEWI VHGHLWDGAREFIIQRNKNVPVNHRDFWYATYTLYSVSEKTENEEKMSDNASASSGSDQG PSSRQHTMVSFLKPVLKQIIMAGKSMQLLKNLQCAESTTCQAGARDAERKSLYTLFLESV QSRLRHGEDSTPQVLTEQQATKENLMKMQSIAESHLELDDVHDPLLAINFARMYLEQSDF HEKFAGGDVCVDRSSESVTCQTFELTLRSCLYPHIDKQYLDCCGNLMQTLKKDYRLVEYL QAMRNFFLMEGGDTMYDFYTSIFDKIREKETWQNVSFLNVQLQEAVGQRYPEDSSRLSIS FENVDTAKKKLPVHILDGLTLSYKVPWPVDIVISLECQKIYNQVFLLLLQIKWAKYSLDV LLFGELVSTAEKPRLKEGLIHEQDTVAQFGPQKEPVRQQIHRMFLLRVKLMHFVNSLHNY IMTRILHSTGLEFQHQVEEAKDLDQLIKIHYRYLSTIHDRCLLREKVSFVKEAIMKVLNL ALMFADGWQAGLGTWRMESIEKMESDFKNCHMFLVTILNKAVCRGSFPHLESLALSLMAG MEQS
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223
Synonyms:
pDONR223, pENTR223-XnCDSYn
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp):
2815
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Mark Vidal
Publications:
PMID:
15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
bacterial origin of replication
2220
2753
gene fragment
pas
5p stem of pBR primose assembly site
63
73
gene fragment
pas prime
3p stem of pBR pas(L)
105
116
recombination site
attL1
attL1 recombination site
570
665
recombination site
attL2
attL2 recombination site
793
696
selectable marker
SpnR
spectinomycin resistance gene
1062
2072
trxn termination sequence
T1-T2
rrnBT1 and T2 terminators
510
224
