Gene:
Gene Symbol:
CATSPERB
Gene Name:
catsper channel auxiliary subunit beta
Original Clone ID:
ccsbBroadEn_04132
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC126214.1,BC126216.1
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00516416
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGAATCGCCACTTATATATGTTTCAGTTTTGCTTTTGAACATATTTGAATTTTCATCA
GGAATAGTATATAATAAAGATGATACAGAGAAACGCTTTGCATGTTCTAACAAAGGGTTC
CCTCAAGAGAATGAAATAATCAAGTTGTATCTTTTCTTAGAAAACTTGAAAATCCAGTGT
TTCTTCCAAACTGAAAATGAAATTGCATCAAAAGCAATGCTAAGTGTGTTCACATCAGGA
GGACTTGCTCCCAGCTTGGGAATCATGAATAGTACATATAATGGCATCTTCCACTTTAAT
TTAACGTTGTTCAGTGATCGGATTTTGTGGTTGGTTGATATTCCTAGAGAAAACATCACA
CAAAGCACAGATATTGCAGCTGTAGAAGAATGGTTAGTAAGAATCACTTTACATCATGGA
CTAAATATTTATGCTACTGAAGGAACTCTATTGGATGTTATTCGAGAACCGATTCTTCAG
TGGACTCCTGGGGATGTGATTCCAGAAAGTGAAATCAGTAAATTATATCCACATGTGGTA
GATCTCAAAGTGACAAAATGCCCCTGTGCCAATGATGTGGCATTACTAGGCTTCATTGTG
GATACAATAGTTGATGGTGTTTACATAGGCATAACCTTTGGTGGATTCTGGCATGATTAT
GATACCACATGGTTTAACATGACACAGACTATCTATTCCCAACTTCAAGAAGAATATGAA
GACCTTTCATTGGTGGATATGGTTTTAACGAATCATTTTTTAGTTATCCTCACCTCTTTG
GGCCTTTTTGTAAGTGAAGATCTTCGTTATCCATCACGCCACAGCTTATCGTTTTCCAGG
GCAGACTTTTGTGGTTTTGAAAGGGTTGACTATGTGAAAGGAAAACTGTGGTATAATGAA
AGATGTTTTGCTAACAGAGAGCACTTTGAAGTTGATTATGTTACAGTTACCTTTGAGAGA
AACAGAACCCTAAGTGAGTCAAGCTCTTGTTTTTATAGTCAGGAACCATTTCTTGAATGG
GTACCCTGCTTACCTCACATTTTTAAAGGAATAAAAATTTTTCCAACTGTGCTAACATTT
CTTGTTGACCAAGAGCGTGGTACTGGAGTTTACCTCTTCTATAACAAGGTCAGGAAAACT
GCCATTGCCTCTGTGAGCACCCTGAGAAATAATGAACCAAATTCACAATCAAAATTTCCA
ATTTTTCGGTTTCCTTCATCATTCTCTTCTCCCGTTGGAATGGTATTTCATCCCCGAAGC
CACTTTTTGTATGCTTATGGCAATCAGATATGGCTTTCAGTTGATGGCGGCAACACCTTT
CAATTAATAGCTAACTTTCATGATGATATCATAAAGAAGACTTTTCATAGTTTTTATACA
TCAGCTATTACTTTTGTTTCTCAACGTGGAAAGGTTTACTCGACAAAGGCAGGAATGGGA
AGATACAGTGCAGTCGGAAGTGTTACTGAGAGAATTTTCACATTATACTATGATCACTTG
GGATTCCTACATAAGCTGACTCTGGGTCGCTTTGAAGCTAGTGGACCACCCACAGCCTTT
GGAAATTCTAGAAATCTTTTTGGACAGCCTCCAGATATGGGCTTTGAGACTGCGCTTGCC
CCACAGCACACCTCCTTAGATGAAATTATCTTTTTTGCATATGTACCTGAGAACGAACCC
CAGGAAACGATCTACAGCAAGAAGTTCGGCAATATACACTATGGAAAAGTGATACACTCT
GGGAAAACTGGAAGAGCTTACATAAGAAAGGTATTGCAACATACGACTCCTAAAGGATTT
TTGTCCTCAGTTATTGCAGAAATGAAAGAGCCCTTTGGATTAGAAGAAGTGAATGAGAGC
TCTTGTTTGTCTAGTTCCCTTTTGATTAATAAAGCTGGAAATGTCTATAAACTCACTCTT
GATTCACAAGTTGTTCAGGCCTTGTTTGAAGATACAGATATAGAGAAGACTGTAGTGCTT
CCCGGGTACAGCAGCTTCCTCATCACAAGCATTTTAGATAATAAGAATGCATTAGCCATT
GCTACCATGCCTGAAAGTGCACCCAACAATATGACCTTTCTAAAGAGCACATGGTTCTTA
TACAACTTTGGGCAAAGGAATGGACGAACATGGAAAATATATTCAAAACCATGTAATTAT
TGGTTTCAACATGATGATTCACCATCCCTCAACATTGTGAAATACATTGATCTGGGAAAC
TCTTATGTTTTAAAAGCTAAGGTCATACGGAATGCAAAAGGTTTTCGAATGCTTGAAATA
CCACTACTGACTGTGTTTGTTGGAAACCCTAATTTGTTGGAAGTTACAGCTGAAGTCACT
TTTGATGATACTGACAGTTATGTAATAACAATTTCTGCAGCTAGCAAAGTTTTACATCAG
GGTTCAACTTCACTGGCATTTATTATGTGGTCAGCCTCTACTGAGTGCTTTGTTACGACA
ATGGTGCCAACACTGAAAAGCAGCTGTAGTTATCTCAGATCTATGCATCACATTCCTAGC
AAATTTATCCCATTTGAAGACTGGATTAGTGGAGTTCATAAAGACAGTCAGGGTTTTAAC
CTCATCAAAACTTTGCCGATAAACTACAGGCCTCCATCTAATATGGGAATTGCTATTCCA
CTCACAGATAATTTTTATCATGCAGATCCTAGCAAACCCATACCAAGAAACATGTTTCAC
ATGTCAAAGAAAACCGGTAAATTCAAACAGTGTGCTAATGTTTCCACTCGGGAGGAGTGT
AACTGCACAAAGGATCAGAAGTTTTCACATGCTGTTGCTTTCTCGGATTGCAGGGAAAAA
GTTCCTCGCTTTAAGTTTCCAATTACACAATATCCAGTTTCTTTGGAAATTATCAACGAG
GATGGACGTGTCCCATTGCAATCTCCATATCTGGTTACTGTGACTGAAGTGAACATGAGG
CACAACTGGAAACTGAAACACACTGTGCCAGAAAATATTAAAAGAATGAAACAATTAGTA
GAACCAATTCTTGGTGCTGCAGTGTATAATCCTTCAGGTCTCAACTTAAGCATAAAGGGC
TCTGAACTTTTCCACTTTAGAGTGACCGTCATTTCAGGAGTAACTTTTTGTAACTTAATT
GAAGAATTTCAGATTTATGTTGATGAGGCACCATTGCCATTCCCAGGACACACGCTTATT
GCCGTGGCAACAGCGGTAGTGCTAGGGGGATTAATTTTTATAGCATTTATGTTTCAACTG
CAAGGCATCCATCCGTGGAGGACATTCCAAAGATGGATTAGAAGAAACCAAGAGAAGTTT
TCAAGTATCTCTCTCAGTGAGCTGATTCATAGATCAAAGTCTGAAGAG
Protein sequence predicted based on nucleotide sequence
MESPLIYVSVLLLNIFEFSSGIVYNKDDTEKRFACSNKGFPQENEIIKLYLFLENLKIQC FFQTENEIASKAMLSVFTSGGLAPSLGIMNSTYNGIFHFNLTLFSDRILWLVDIPRENIT QSTDIAAVEEWLVRITLHHGLNIYATEGTLLDVIREPILQWTPGDVIPESEISKLYPHVV DLKVTKCPCANDVALLGFIVDTIVDGVYIGITFGGFWHDYDTTWFNMTQTIYSQLQEEYE DLSLVDMVLTNHFLVILTSLGLFVSEDLRYPSRHSLSFSRADFCGFERVDYVKGKLWYNE RCFANREHFEVDYVTVTFERNRTLSESSSCFYSQEPFLEWVPCLPHIFKGIKIFPTVLTF LVDQERGTGVYLFYNKVRKTAIASVSTLRNNEPNSQSKFPIFRFPSSFSSPVGMVFHPRS HFLYAYGNQIWLSVDGGNTFQLIANFHDDIIKKTFHSFYTSAITFVSQRGKVYSTKAGMG RYSAVGSVTERIFTLYYDHLGFLHKLTLGRFEASGPPTAFGNSRNLFGQPPDMGFETALA PQHTSLDEIIFFAYVPENEPQETIYSKKFGNIHYGKVIHSGKTGRAYIRKVLQHTTPKGF LSSVIAEMKEPFGLEEVNESSCLSSSLLINKAGNVYKLTLDSQVVQALFEDTDIEKTVVL PGYSSFLITSILDNKNALAIATMPESAPNNMTFLKSTWFLYNFGQRNGRTWKIYSKPCNY WFQHDDSPSLNIVKYIDLGNSYVLKAKVIRNAKGFRMLEIPLLTVFVGNPNLLEVTAEVT FDDTDSYVITISAASKVLHQGSTSLAFIMWSASTECFVTTMVPTLKSSCSYLRSMHHIPS KFIPFEDWISGVHKDSQGFNLIKTLPINYRPPSNMGIAIPLTDNFYHADPSKPIPRNMFH MSKKTGKFKQCANVSTREECNCTKDQKFSHAVAFSDCREKVPRFKFPITQYPVSLEIINE DGRVPLQSPYLVTVTEVNMRHNWKLKHTVPENIKRMKQLVEPILGAAVYNPSGLNLSIKG SELFHFRVTVISGVTFCNLIEEFQIYVDEAPLPFPGHTLIAVATAVVLGGLIFIAFMFQL QGIHPWRTFQRWIRRNQEKFSSISLSELIHRSKSEE
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223
Synonyms:
pDONR223, pENTR223-XnCDSYn
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp):
2815
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Mark Vidal
Publications:
PMID:
15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
bacterial origin of replication
2220
2753
gene fragment
pas
5p stem of pBR primose assembly site
63
73
gene fragment
pas prime
3p stem of pBR pas(L)
105
116
recombination site
attL1
attL1 recombination site
570
665
recombination site
attL2
attL2 recombination site
793
696
selectable marker
SpnR
spectinomycin resistance gene
1062
2072
trxn termination sequence
T1-T2
rrnBT1 and T2 terminators
510
224
