Gene:
Gene Symbol:
MAML2
Gene Name:
mastermind-like 2 (Drosophila)
Original Clone ID:
ccsbBroadEn_12817
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC152449.1
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00516419
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGGGGACACAGCGCCCCCGCAGGCCCCCGCAGGAGGGCTAGGGGGGGCCTCTGGGGCG
GGGCTCCTTGGAGGGGGCTCAGTCACCCCGAGAGTGCACAGTGCTATCGTGGAGCGCCTC
CGGGCTCGGATCGCTGTCTGCCGCCAACACCACCTGAGCTGTGAAGGACGATATGAACGA
GGTAGGGCCGAGAGCTCAGACCGGGAAAGAGAAAGCACCTTGCAGCTCCTGAGCCTTGTA
CAGCATGGCCAGGGGGCAAGGAAAGCTGGCAAACACACCAAGGCCACCGCCACTGCTGCC
ACCACTACAGCCCCTCCACCGCCCCCTGCTGCCCCTCCTGCGGCCTCCCAAGCAGCAGCA
ACAGCAGCCCCACCGCCCCCACCAGACTATCACCATCACCACCAGCAGCACCTGCTGAAC
AGTAGCAATAATGGTGGCAGTGGTGGGATAAACGGAGAGCAGCAGCCGCCCGCTTCAACC
CCAGGGGACCAGAGGAACTCAGCCCTGATTGCGCTCCAGGGTTCCTTGAAAAGAAAACAG
GTAGTTAACCTATCTCCTGCCAACAGCAAGCGACCCAATGGCTTTGTGGACAACTCATTT
CTTGATATCAAAAGAATTCGTGTTGGGGAGAATCTCTCTGCAGGACAAGGTGGCCTCCAA
ATAAACAATGGACAAAGTCAGATTATGTCAGGGACCTTGCCTATGAGCCAAGCACCCCTG
CGAAAGACTAACACTCTGCCATCCCATACACATTCTCCTGGCAATGGCCTGTTTAACATG
GGCTTAAAGGAGGTAAAGAAGGAGCCAGGAGAGACTCTGTCTTGCAGTAAGCACATGGAT
GGCCAAATGACCCAAGAGAATATTTTTCCTAATAGGTACGGAGACGACCCTGGAGAACAA
CTGATGGATCCTGAGCTGCAGGAACTGTTCAATGAACTGACCAACATATCTGTGCCTCCC
ATGAGTGACCTTGAACTGGAGAACATGATCAATGCCACCATAAAGCAGGATGACCCATTT
AACATTGACTTGGGTCAGCAAAGCCAGAGGAGCACACCTAGGCCCTCCTTACCCATGGAG
AAAATAGTGATCAAAAGTGAATACTCACCGGGCTTGACTCAGGGCCCCTCAGGCTCTCCT
CAGCTGAGGCCCCCATCAGCTGGCCCCGCATTCTCCATGGCCAACTCTGCCCTCTCCACT
TCGTCTCCAATCCCTTCAGTCCCTCAGAGCCAGGCTCAGCCTCAGACAGGCTCCGGAGCA
AGCCGGGCCTTGCCAAGCTGGCAGGAAGTATCCCATGCCCAGCAGCTCAAACAGATAGCT
GCTAATCGTCAGCAGCATGCCCGGATGCAGCAGCACCAGCAGCAGCACCAGCCTACCAAC
TGGTCAGCCTTGCCCTCCTCTGCTGGACCATCACCAGGTCCATTTGGGCAGGAGAAAATC
CCCAGCCCTTCTTTTGGTCAGCAGACATTCAGCCCACAGAGCTCCCCCATGCCTGGGGTA
GCTGGCGGCAGCGGCCAGTCGAAAGTAATGGCTAACTACATGTACAAGGCCGGCCCCTCA
GCCCAGGGTGGGCACCTAGATGTCCTCATGCAGCAAAAGCCTCAGGATCTCAGTCGAAGT
TTTATTAACAACCCGCACCCAGCCATGGAGCCCCGTCAGGGCAACACCAAGCCTTTGTTT
CATTTTAACTCAGATCAAGCGAACCAGCAGATGCCTTCTGTTTTGCCTTCCCAGAACAAG
CCTTCTCTCCTACACTACACCCAACAGCAACAGCAGCAACAGCAGCAGCAGCAGCAGCAG
CAGCAGCAGCAACAGCAGCAGCAGCAGCAACAGCAACAGCAACAGCAACAGCAGAGTTCA
ATTTCAGCTCAACAACAGCAACAGCAGCAGAGCTCAATTTCAGCCCAACAGCAGCAGCAG
CAGCAACAACAGCAGCAGCAGCAGCAACAACAACAGCAACAACAGCAGCAGCAGCAGCAG
CAACAACCATCTTCTCAGCCTGCCCAATCTCTACCAAGCCAGCCTTTGCTAAGGTCACCT
TTGCCACTTCAGCAAAAGCTCCTACTTCAGCAAATGCAGAATCAGCCCATTGCAGGAATG
GGATACCAAGTCTCCCAACAACAGAGACAGGATCAACACTCTGTGGTAGGCCAGAACACA
GGCCCCAGTCCAAGTCCTAACCCCTGCTCAAATCCAAACACTGGAAGTGGTTACATGAAC
TCCCAGCAATCACTGTTGAATCAGCAATTGATGGGAAAGAAGCAGACTCTACAGAGGCAG
ATCATGGAGCAGAAACAGCAACTTCTTCTCCAGCAGCAGATGCTGGCTGACGCGGAGAAA
ATTGCTCCACAAGATCAGATAAACCGACATTTGTCAAGGCCACCTCCAGATTATAAAGAC
CAAAGAAGAAATGTGGGCAATATGCAACCAACTGCTCAGTATTCTGGTGGCTCATCCACA
ATAAGCTTAAACTCTAACCAGGCTTTGGCAAACCCAGTTTCAACACACACCATTTTAACT
CCCAATTCCAGCCTCCTGTCTACTTCTCACGGGACAAGAATGCCATCATTATCTACAGCA
GTTCAGAATATGGGGATGTATGGAAATCTGCCTTGTAATCAACCTAACACATACAGTGTC
ACTTCAGGAATGAATCAATTGACCCAACAGAGAAACCCAAAGCAATTGTTAGCAAATCAA
AACAACCCTATGATGCCACGGCCACCTACCTTAGGGCCAAGTAATAATAACAATGTAGCC
ACTTTTGGAGCTGGATCTGTTGGTAATTCACAACAATTGAGACCAAATTTAACCCATAGT
ATGGCAAGCATGCCACCACAGAGAACATCAAACGTAATGATCACATCCAACACAACTGCA
CCAAACTGGGCCTCTCAAGAAGGAACAAGCAAACAGCAAGAAGCCCTGACGTCTGCAGGA
GTCCGCTTCCCCACAGGTACACCTGCAGCCTATACCCCAAATCAGTCACTGCAACAGGCA
GTAGGTAGCCAGCAATTTTCCCAGAGGGCAGTGGCTCCTCCTAACCAGTTAACACCAGCA
GTGCAAATGAGACCCATGAACCAAATGAGCCAAACACTAAATGGGCAAACCATGGGTCCC
CTCAGGGGTCTGAATCTCAGACCCAATCAGCTAAGCACACAGATTTTGCCTAATTTGAAT
CAGTCAGGAACAGGGTTGAATCAGTCGAGGACGGGCATCAACCAGCCACCATCCCTGACG
CCCAGCAATTTTCCTTCACCCAACCAAAGTTCCAGGGCTTTTCAAGGAACTGACCACAGC
AGTGACTTAGCTTTTGACTTCCTCAGCCAACAAAATGATAACATGGGCCCTGCCCTAAAC
AGTGATGCTGATTTCATTGATTCTTTATTGAAGACAGAGCCTGGTAATGATGACTGGATG
AAAGACATCAATCTTGATGAAATCTTGGGGAACAATTCC
Protein sequence predicted based on nucleotide sequence
MGDTAPPQAPAGGLGGASGAGLLGGGSVTPRVHSAIVERLRARIAVCRQHHLSCEGRYER GRAESSDRERESTLQLLSLVQHGQGARKAGKHTKATATAATTTAPPPPPAAPPAASQAAA TAAPPPPPDYHHHHQQHLLNSSNNGGSGGINGEQQPPASTPGDQRNSALIALQGSLKRKQ VVNLSPANSKRPNGFVDNSFLDIKRIRVGENLSAGQGGLQINNGQSQIMSGTLPMSQAPL RKTNTLPSHTHSPGNGLFNMGLKEVKKEPGETLSCSKHMDGQMTQENIFPNRYGDDPGEQ LMDPELQELFNELTNISVPPMSDLELENMINATIKQDDPFNIDLGQQSQRSTPRPSLPME KIVIKSEYSPGLTQGPSGSPQLRPPSAGPAFSMANSALSTSSPIPSVPQSQAQPQTGSGA SRALPSWQEVSHAQQLKQIAANRQQHARMQQHQQQHQPTNWSALPSSAGPSPGPFGQEKI PSPSFGQQTFSPQSSPMPGVAGGSGQSKVMANYMYKAGPSAQGGHLDVLMQQKPQDLSRS FINNPHPAMEPRQGNTKPLFHFNSDQANQQMPSVLPSQNKPSLLHYTQQQQQQQQQQQQQ QQQQQQQQQQQQQQQQQQSSISAQQQQQQQSSISAQQQQQQQQQQQQQQQQQQQQQQQQQ QQPSSQPAQSLPSQPLLRSPLPLQQKLLLQQMQNQPIAGMGYQVSQQQRQDQHSVVGQNT GPSPSPNPCSNPNTGSGYMNSQQSLLNQQLMGKKQTLQRQIMEQKQQLLLQQQMLADAEK IAPQDQINRHLSRPPPDYKDQRRNVGNMQPTAQYSGGSSTISLNSNQALANPVSTHTILT PNSSLLSTSHGTRMPSLSTAVQNMGMYGNLPCNQPNTYSVTSGMNQLTQQRNPKQLLANQ NNPMMPRPPTLGPSNNNNVATFGAGSVGNSQQLRPNLTHSMASMPPQRTSNVMITSNTTA PNWASQEGTSKQQEALTSAGVRFPTGTPAAYTPNQSLQQAVGSQQFSQRAVAPPNQLTPA VQMRPMNQMSQTLNGQTMGPLRGLNLRPNQLSTQILPNLNQSGTGLNQSRTGINQPPSLT PSNFPSPNQSSRAFQGTDHSSDLAFDFLSQQNDNMGPALNSDADFIDSLLKTEPGNDDWM KDINLDEILGNNS
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223
Synonyms:
pDONR223, pENTR223-XnCDSYn
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp):
2815
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Mark Vidal
Publications:
PMID:
15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
bacterial origin of replication
2220
2753
gene fragment
pas
5p stem of pBR primose assembly site
63
73
gene fragment
pas prime
3p stem of pBR pas(L)
105
116
recombination site
attL1
attL1 recombination site
570
665
recombination site
attL2
attL2 recombination site
793
696
selectable marker
SpnR
spectinomycin resistance gene
1062
2072
trxn termination sequence
T1-T2
rrnBT1 and T2 terminators
510
224
