Gene:
Gene Symbol:
INO80
Gene Name:
INO80 complex subunit
Original Clone ID:
ccsbBroadEn_03442
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC146785.1
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00516448
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGCCTCGGAGTTGGGTGCCAGGGATGATGGAGGCTGCACTGAGCTGGCAAAGCCCCTC
TATCTTCAGTACTTGGAGAGGGCCCTCCGGTTGGACCATTTTCTGCGACAAACGTCAGCT
ATCTTCAATAGGAATATTTCTAGTGATGACAGTGAAGATGGACTGGATGACAGTAATCCA
TTATTGCCCCAGTCTGGGGATCCCTTAATACAAGTTAAGGAAGAACCTCCAAATTCATTG
CTTGGTGAAACTTCTGGAGCAGGCAGTTCTGGAATGTTAAACACATATTCTCTGAATGGA
GTTCTACAGTCAGAATCAAAATGTGATAAGGGGAATTTATATAATTTCTCTAAGCTGAAG
AAAAGCAGAAAGTGGCTAAAGAGCATTCTGCTAAGTGATGAATCCAGCGAGGCTGATTCT
CAGAGTGAAGACGATGATGAAGAAGAACTCAATCTCAGCAGAGAAGAACTTCACAACATG
CTTCGACTACACAAATATAAGAAACTTCACCAAAATAAGTATAGTAAAGACAAGGAGTTG
CAGCAATATCAGTACTACAGTGCAGGCCTGCTCTCCACATATGACCCTTTCTATGAGCAA
CAACGGCACCTACTTGGACCCAAGAAAAAGAAATTTAAGGAGGAAAAGAAACTTAAAGCT
AAGTTGAAAAAAGTGAAGAAAAAAAGACGAAGAGATGAAGAACTTTCCTCTGAAGAATCC
CCTCGTCGCCATCACCACCAGACCAAAGTCTTTGCCAAGTTTTCTCACGATGCACCTCCC
CCTGGCACTAAGAAAAAGCACTTATCCATTGAGCAGCTGAATGCTCGTCGCAGGAAAGTA
TGGCTCAGCATTGTGAAAAAGGAACTACCAAAGGCAAATAAGCAGAAAGCTTCAGCTCGT
AACCTGTTTCTCACCAATAGCCGAAAGCTTGCTCACCAGTGCATGAAGGAGGTGCGTCGA
GCTGCCTTGCAGGCCCAGAAGAACTGTAAGGAAACCTTGCCTCGTGCCCGCCGCCTCACC
AAGGAGATGCTTCTGTACTGGAAGAAATATGAGAAAGTAGAGAAGGAGCACCGCAAGAGA
GCAGAGAAGGAAGCTTTGGAGCAGCGGAAGTTGGATGAGGAAATGCGGGAGGCCAAGAGG
CAACAGCGAAAACTCAACTTCTTAATTACCCAGACAGAGTTGTATGCCCATTTCATGAGT
CGCAAACGAGATATGGGTCATGATGGTATCCAGGAAGAAATCCTAAGGAAACTGGAAGAC
AGTTCTACCCAGAGACAAATCGATATAGGTGGAGGAGTGGTAGTTAACATCACACAGGAG
GATTATGATAGTAACCATTTTAAAGCCCAGGCCCTGAAGAATGCTGAAAATGCTTACCAT
ATTCACCAAGCTCGGACAAGGTCATTTGATGAAGATGCAAAAGAAAGTCGAGCAGCTGCC
CTACGGGCAGCAAACAAGTCTGGCACTGGGTTTGGGGAGAGTTATAGCCTGGCTAACCCA
TCTATCCGGGCTGGTGAGGATATTCCACAGCCCACAATTTTTAATGGCAAATTGAAAGGT
TATCAACTGAAAGGCATGAATTGGTTGGCCAATCTATATGAACAGGGTATTAATGGCATT
CTTGCTGATGAAATGGGCCTTGGTAAAACAGTACAGAGCATTGCCCTTCTGGCCCATCTG
GCTGAGAGAGAGAACATTTGGGGACCTTTCTTAATAATTTCACCTGCGTCTACACTTAAC
AATTGGCACCAGGAGTTTACTAGATTTGTTCCTAAATTTAAGGTGCTACCATATTGGGGA
AATCCTCATGATAGAAAAGTCATCAGAAGGTTCTGGAGTCAGAAGACCCTCTACACTCAG
GATGCCCCCTTCCATGTGGTTATTACCAGCTATCAGCTGGTGGTGCAGGATGTAAAGTAT
TTCCAGCGGGTCAAGTGGCAATACATGGTACTGGATGAGGCTCAGGCGCTCAAGAGTAGT
TCCAGTGTTCGTTGGAAGATCCTCTTACAGTTCCAGTGTCGGAATCGGCTTTTGCTAACC
GGGACCCCAATTCAGAACACCATGGCAGAGCTTTGGGCTCTGCTGCATTTCATTATGCCA
ACATTATTTGATTCACATGAGGAATTTAATGAATGGTTTTCCAAGGACATTGAGAGCCAT
GCCGAAAACAAATCTGCTATTGATGAGAATCAACTTTCTCGCTTACACATGATTTTGAAG
CCATTTATGCTGAGGAGAATCAAGAAAGATGTGGAAAATGAATTATCTGACAAGATTGAG
ATTCTAATGTATTGCCAACTGACCAGCCGACAGAAGCTGCTATATCAGGCACTAAAGAAC
AAAATTTCCATTGAGGATTTATTGCAGTCTTCTATGGGCTCTACCCAACAAGCACAGAAC
ACCACCAGCAGCCTCATGAATCTGGTCATGCAGTTTAGGAAGGTGTGTAATCACCCGGAG
TTATTTGAACGGCAAGAAACTTGGTCTCCATTTCATATTTCCCTAAAGCCATACCACATT
TCAAAGTTTATCTACCGTCATGGACAGATCAGGGTCTTCAATCATTCACGAGACAGGTGG
TTAAGGGTTCTTTCTCCATTTGCACCAGACTATATCCAACGGTCTCTCTTTCACAGAAAA
GGTATTAATGAAGAAAGCTGTTTCTCTTTCCTTCGCTTTATTGATATATCTCCAGCAGAA
ATGGCAAACCTTATGCTTCAGGGACTTTTGGCCAGATGGTTAGCTCTTTTCCTGTCTCTG
AAAGCCTCCTACAGGCTCCATCAGCTACGCTCCTGGGGAGCGCCAGAAGGGGAGAGCCAC
CAGAGATACCTGAGGAACAAGGATTTCCTTCTTGGGGTTAATTTTCCACTCTCCTTTCCA
AACCTTTGCAGCTGCCCTTTGTTAAAGTCTCTTGTTTTCAGCAGCCACTGTAAAGCAGTG
AGTGGCTACTCAGACCAGGTTGTCCATCAGCGGAGATCAGCTACCTCCTCGCTGCGTCGC
TGCCTGCTCACTGAGCTGCCATCTTTTTTGTGTGTGGCCAGTCCACGAGTTACCGCAGTG
CCATTGGATTCTTACTGCAATGACCGAAGTGCAGAATATGAAAGGCGAGTTCTGAAGGAA
GGAGGGAGTCTGGCAGCCAAGCAGTGTTTGTTGAATGGGGCCCCTGAACTGGCTGCAGAC
TGGCTAAATAGACGATCACAGTTCTTCCCAGAGCCAGCTGGAGGTCTGTGGAGCATCAGA
CCTCAGAATGGCTGGTCTTTCATCAGGATTCCAGGCAAGGAGAGCCTCATCACTGACAGT
GGAAAGCTGTATGCCCTTGATGTCCTGCTGACTCGGCTCAAGTCTCAAGGGCATAGGGTC
CTTATCTACTCCCAGATGACCAGGATGATAGACCTACTGGAGGAATACATGGTTTACAGG
AAGCATACCTACATGAGGCTTGATGGCTCATCCAAGATCTCGGAGAGGCGAGACATGGTT
GCTGATTTTCAGAACAGGAATGACATCTTTGTGTTCCTGTTAAGCACACGAGCTGGAGGA
CTGGGTATCAATCTCACTGCTGCAGACACAGTGATTTTCTATGATAGCGACTGGAACCCC
ACTGTGGACCAGCAGGCCATGGACAGGGCCCACCGCTTAGGGCAGACAAAGCAGGTTACT
GTGTACCGGCTCATCTGTAAAGGCACCATTGAAGAACGCATTCTGCAAAGAGCCAAGGAG
AAGAGTGAGATTCAGCGGATGGTGATTTCAGGTGGGAACTTCAAACCAGATACCTTGAAA
CCCAAAGAGGTGGTTAGTCTTCTTCTAGACGACGAAGAGTTGGAGAAGAAACTGAGGCTG
CGGCAGGAAGAGAAACGGCAACAGGAGGAAACCAACCGAGTGAAAGAGCGCAAGCGGAAG
CGGGAAAAGTATGCAGAGAAGAAGAAAAAAGAAGATGAATTGGATGGGAAAAGGAGAAAA
GAGGGTGTGAACCTGGTGATCCCATTTGTTCCCTCGGCTGATAACTCCAACCTCTCTGCT
GACGGAGATGACTCCTTCATTAGCGTGGACTCAGCCATGCCAAGCCCTTTCAGTGAGATC
TCCATCAGCAGTGAGCTGCACACTGGCTCCATTCCCCTGGACGAGAGCAGCAGTGACATG
CTGGTCATTGTGGATGACCCAGCCTCCTCAGCCCCTCAGTCTCGAGCTACCAACTCTCCC
GCATCCATAACAGGCTCCGTCTCAGATACCGTGAATGGAATTTCCATTCAGGAAATGCCA
GCTGCAGGACGTGGTCACTCAGCCCGAAGCCGAGGCCGCCCCAAAGGTTCAGGAAGCACA
GCCAAAGGAGCAGGGAAGGGCCGGAGCCGAAAGTCCACGGCAGGCAGTGCTGCTGCAATG
GCAGGAGCCAAAGCCGGGGCTGCAGCGGCCTCTGCAGCTGCCTATGCCGCATACGGGTAC
AACGTGTCTAAAGGAATCTCCGCCAGCAGTCCTCTGCAGACATCCCTTGTTCGGCCTGCT
GGCCTTGCTGACTTTGGACCTTCAAGCGCCTCTTCTCCTTTGAGTTCCCCTTTGAGCAAG
GGAAATAATGTTCCTGGGAATCCCAAGAACCTCCACATGACCAGCAGCCTAGCCCCAGAC
TCTCTGGTCCGGAAACAGGGCAAAGGCACCAACCCCTCTGGAGGACGG
Protein sequence predicted based on nucleotide sequence
MASELGARDDGGCTELAKPLYLQYLERALRLDHFLRQTSAIFNRNISSDDSEDGLDDSNP LLPQSGDPLIQVKEEPPNSLLGETSGAGSSGMLNTYSLNGVLQSESKCDKGNLYNFSKLK KSRKWLKSILLSDESSEADSQSEDDDEEELNLSREELHNMLRLHKYKKLHQNKYSKDKEL QQYQYYSAGLLSTYDPFYEQQRHLLGPKKKKFKEEKKLKAKLKKVKKKRRRDEELSSEES PRRHHHQTKVFAKFSHDAPPPGTKKKHLSIEQLNARRRKVWLSIVKKELPKANKQKASAR NLFLTNSRKLAHQCMKEVRRAALQAQKNCKETLPRARRLTKEMLLYWKKYEKVEKEHRKR AEKEALEQRKLDEEMREAKRQQRKLNFLITQTELYAHFMSRKRDMGHDGIQEEILRKLED SSTQRQIDIGGGVVVNITQEDYDSNHFKAQALKNAENAYHIHQARTRSFDEDAKESRAAA LRAANKSGTGFGESYSLANPSIRAGEDIPQPTIFNGKLKGYQLKGMNWLANLYEQGINGI LADEMGLGKTVQSIALLAHLAERENIWGPFLIISPASTLNNWHQEFTRFVPKFKVLPYWG NPHDRKVIRRFWSQKTLYTQDAPFHVVITSYQLVVQDVKYFQRVKWQYMVLDEAQALKSS SSVRWKILLQFQCRNRLLLTGTPIQNTMAELWALLHFIMPTLFDSHEEFNEWFSKDIESH AENKSAIDENQLSRLHMILKPFMLRRIKKDVENELSDKIEILMYCQLTSRQKLLYQALKN KISIEDLLQSSMGSTQQAQNTTSSLMNLVMQFRKVCNHPELFERQETWSPFHISLKPYHI SKFIYRHGQIRVFNHSRDRWLRVLSPFAPDYIQRSLFHRKGINEESCFSFLRFIDISPAE MANLMLQGLLARWLALFLSLKASYRLHQLRSWGAPEGESHQRYLRNKDFLLGVNFPLSFP NLCSCPLLKSLVFSSHCKAVSGYSDQVVHQRRSATSSLRRCLLTELPSFLCVASPRVTAV PLDSYCNDRSAEYERRVLKEGGSLAAKQCLLNGAPELAADWLNRRSQFFPEPAGGLWSIR PQNGWSFIRIPGKESLITDSGKLYALDVLLTRLKSQGHRVLIYSQMTRMIDLLEEYMVYR KHTYMRLDGSSKISERRDMVADFQNRNDIFVFLLSTRAGGLGINLTAADTVIFYDSDWNP TVDQQAMDRAHRLGQTKQVTVYRLICKGTIEERILQRAKEKSEIQRMVISGGNFKPDTLK PKEVVSLLLDDEELEKKLRLRQEEKRQQEETNRVKERKRKREKYAEKKKKEDELDGKRRK EGVNLVIPFVPSADNSNLSADGDDSFISVDSAMPSPFSEISISSELHTGSIPLDESSSDM LVIVDDPASSAPQSRATNSPASITGSVSDTVNGISIQEMPAAGRGHSARSRGRPKGSGST AKGAGKGRSRKSTAGSAAAMAGAKAGAAAASAAAYAAYGYNVSKGISASSPLQTSLVRPA GLADFGPSSASSPLSSPLSKGNNVPGNPKNLHMTSSLAPDSLVRKQGKGTNPSGGR
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223
Synonyms:
pDONR223, pENTR223-XnCDSYn
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp):
2815
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Mark Vidal
Publications:
PMID:
15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
bacterial origin of replication
2220
2753
gene fragment
pas
5p stem of pBR primose assembly site
63
73
gene fragment
pas prime
3p stem of pBR pas(L)
105
116
recombination site
attL1
attL1 recombination site
570
665
recombination site
attL2
attL2 recombination site
793
696
selectable marker
SpnR
spectinomycin resistance gene
1062
2072
trxn termination sequence
T1-T2
rrnBT1 and T2 terminators
510
224
