Gene:
Gene Symbol:
SNCAIP
Gene Name:
synuclein, alpha interacting protein
Original Clone ID:
ccsbBroadEn_11395
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC040552.1
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00516474
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGAAGCCCCTGAATACCTTGATTTGGATGAAATTGACTTTAGTGATGACATATCTGAC
AATAGGAGTCAAGGGAACAGGCTACAAAAGCTTGGATTGGAGGACACAGACAGGGAAGAT
GCAATGGGCTTTGGTTCCCATAGGGCCAAACTGACAGTAGTTGCTGCCCTGGGAGCTTGC
CATTGTCCTGAGAATGAGTATTCAGTCACATCACTCAAGACGATCCCAGAACTGTGCCGA
AGATGTGATACGCAAAACGAAGACAGATCAGTTTCTAGCTCTAGCTGGAATTGTGGCATC
TCAACTCTTATTACAAACACGCAAAAGCCCACAGGAATCGCTGATGTGTACAGTAAGTTC
CGCCCAGTGAAGCGGGTTTCGCCACTGAAACATCAGCCAGAGACTCTGGAGAACAATGAA
AGTGATGACCAAAAGAACCAGAAAGTGGTTGAGTACCAGAAAGGGGGTGAGTCTGACCTG
GGCCCCCAGCCTCAGGAGCTTGGCCCTGGAGATGGAGTGGGCGGCCCACCAGGTAAGAGC
TCTGAGCCCAGCACATCGCTGGGTGAACTGGAGCACTACGACCTCGACATGGATGAGATT
CTGGATGTGCCTTATATTAAATCCAGTCAGCAGCTTGCCTCTTTTACCAAGGTGACTTCA
GAAAAAAGAATTTTGGGCTTATGCACAACCATCAATGGCCTTTTTGGCAAAGCCTGCTCT
ACAGGAAGTTCTGAGAGCTCATCATCCAACATGGCACCATTTTGTGTTCTTTCTCCCGTG
AAAAGCCCTCACTTGAGAAAAGCATCAGCTGTCATCCACGACCAGCACAAGCTGTCCACT
GAAGAAACCGAGATCTCACCTCCTCTGGTTAAATGTGGCTCTGCATATGAGCCTGAAAAC
CAGAGTAAAGACTTCCTAAACAAGACATTTAGTGATCCTCATGGTCGAAAAGTTGAGAAG
ACAACACCAGACTGCCAGCTCAGGGCCTTCCACCTACAATCCTCAGCAGCAGAATCCAAA
CCAGAAGAGCAGGTCAGTGGCCTAAACCGGACCAGCTCCCAAGGCCCAGAAGAAAGGAGT
GAGTATCTGAAAAAAGTGAAAAGCATCTTGAACATTGTTAAAGAAGGACAGATCTCTCTC
CTGCCACACCTAGCTGCAGACAATCTAGACAAAATTCACGACGAAAATGGAAACAATCTA
TTACATATTGCGGCGTCACAGGGACACGCAGAGTGTCTACAGCACCTCACTTCTTTGATG
GGAGAAGACTGCCTCAATGAGCGCAACACTGAGAAGTTGACTCCAGCAGGCCTGGCCATT
AAGAATGGTCAGTTGGAGTGCGTACGCTGGATGGTGAGCGAAACAGAAGCCATTGCAGAA
CTGAGTTGTTCTAAGGATTTTCCAAGCCTTATTCATTACGCAGGTTGCTATGGCCAGGAA
AAGATTCTTCTGTGGCTTCTTCAGTTTATGCAAGAGCAGGGCATCTCGTTGGATGAAGTA
GACCAGGATGGCAACAGTGCCGTTCACGTAGCCTCACAGCATGGCTACCTTGGATGCATA
CAGACCTTGGTTGAATATGGAGCAAATGTCACCATGCAGAACCACGCTGGGGAAAAGCCC
TCCCAGAGCGCCGAGCGGCAGGGGCACACCCTGTGCTCCAGGTACCTGGTGGTGGTGGAG
ACCTGCATGTCGCTGGCCTCTCAAGTGGTGAAGTTAACCAAGCAGCTAAAGGAACAAACA
GTAGAACGTGTCACGCTGCAGAACCAACTCCAACAATTTCTAGAAGCCCAGAAATCAGAG
GGCAAGTCACTCCCTTCTTCACCCAGTTCACCATCCTCACCTGCCTCCAGAAAGTCCCAG
TGGAAATCTCCAGATGCAGATGATGATTCTGTAGCCAAAAGCAAGCCAGGAGTCCAAGAG
GGGATTCAGGTTCTTGGAAGCCTGTCAGCCTCCAGCCGGGCTAGACCCAAAGCAAAAGAT
GGAGATTCTGATAAAATCTTACGCCAGTTATTGGGAAAGGAAATCTCAGAAAATGTCTGC
ACCCAGGAAAAACTGTCCTTGGAATTCCAGGATGCTCAGGCTTCCTCTAGAAATTCTAAA
AAGATCCCACTGGAGAAGAGGGAACTGAAGTTAGCCAGGCTGAGACAGCTGATGCAGAGG
TCACTGAGTGAGTCTGACACAGACTCCAACAACTCTGAGGACCCCAAGACTACCCCAGTG
AGGAAGGGTGACCGACCAAGGCCGCAGCCCATTGTAGAAAGCGTAGAGAGTATGGACAGC
GCAGAAAGCCTGCACCTGATGATTAAGAAACACACCTTGGCATCAGGGGGACGCAGGTTT
CCTTTCAGCATCAAGGCCTCCAAATCCCTGGATGGCCACAGCCCATCTCCCACCTCAGAG
AGCAGCGAACCAGACTTAGAATCCCAGTATCCAGGCTCAGGGAGTATTCCTCCAAACCAG
CCCTCTGGTGACCCTCAGCAGCCCAGCCCTGACAGTACTGCTGCCCAGAAAGTTGCCACA
AGTCCCAAGAGTGCCCTCAAGTCTCCATCTTCCAAGCGTAGGACATCTCAGAACTTAAAA
CTGAGAGTTACCTTTGAGGAGCCTGTGGTGCAGATGGAGCAGCCTAGCCTTGAACTGAAT
GGAGAAAAAGACAAAGATAAGGGCAGGACTCTCCAGCGGACCTCCACAAGTAACGAATCG
GGGGATCAACTGAAAAGGCCTTTTGGAGCCTTTCGATCTATCATGGAGACACTAAGTGGC
AACCAAAACAATAATAATAACTACCAGGCAGCCAACCAGCTGAAAACCTCTACATTGCCC
TTGACCTCACTTGGGAGGAAGACAGATGCCAAGGGAAACCCTGCCAGCTCCGCTAGCAAA
GGAAAGAATAAGGCAGAAATGTACAGCAGCTGCATCAATCTTTCCTCTAACATGCTGATT
GAAGAGCATCTGTGTAACGACACACGGCATAATGACATCAATAGAAAAATGAAGAAATCC
TACAGCATAAAGCACATTGCTGAGCCAGAGTCAAAAGAACTCTTCTTG
Protein sequence predicted based on nucleotide sequence
MEAPEYLDLDEIDFSDDISDNRSQGNRLQKLGLEDTDREDAMGFGSHRAKLTVVAALGAC HCPENEYSVTSLKTIPELCRRCDTQNEDRSVSSSSWNCGISTLITNTQKPTGIADVYSKF RPVKRVSPLKHQPETLENNESDDQKNQKVVEYQKGGESDLGPQPQELGPGDGVGGPPGKS SEPSTSLGELEHYDLDMDEILDVPYIKSSQQLASFTKVTSEKRILGLCTTINGLFGKACS TGSSESSSSNMAPFCVLSPVKSPHLRKASAVIHDQHKLSTEETEISPPLVKCGSAYEPEN QSKDFLNKTFSDPHGRKVEKTTPDCQLRAFHLQSSAAESKPEEQVSGLNRTSSQGPEERS EYLKKVKSILNIVKEGQISLLPHLAADNLDKIHDENGNNLLHIAASQGHAECLQHLTSLM GEDCLNERNTEKLTPAGLAIKNGQLECVRWMVSETEAIAELSCSKDFPSLIHYAGCYGQE KILLWLLQFMQEQGISLDEVDQDGNSAVHVASQHGYLGCIQTLVEYGANVTMQNHAGEKP SQSAERQGHTLCSRYLVVVETCMSLASQVVKLTKQLKEQTVERVTLQNQLQQFLEAQKSE GKSLPSSPSSPSSPASRKSQWKSPDADDDSVAKSKPGVQEGIQVLGSLSASSRARPKAKD GDSDKILRQLLGKEISENVCTQEKLSLEFQDAQASSRNSKKIPLEKRELKLARLRQLMQR SLSESDTDSNNSEDPKTTPVRKGDRPRPQPIVESVESMDSAESLHLMIKKHTLASGGRRF PFSIKASKSLDGHSPSPTSESSEPDLESQYPGSGSIPPNQPSGDPQQPSPDSTAAQKVAT SPKSALKSPSSKRRTSQNLKLRVTFEEPVVQMEQPSLELNGEKDKDKGRTLQRTSTSNES GDQLKRPFGAFRSIMETLSGNQNNNNNYQAANQLKTSTLPLTSLGRKTDAKGNPASSASK GKNKAEMYSSCINLSSNMLIEEHLCNDTRHNDINRKMKKSYSIKHIAEPESKELFL
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223
Synonyms:
pDONR223, pENTR223-XnCDSYn
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp):
2815
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Mark Vidal
Publications:
PMID:
15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
bacterial origin of replication
2220
2753
gene fragment
pas
5p stem of pBR primose assembly site
63
73
gene fragment
pas prime
3p stem of pBR pas(L)
105
116
recombination site
attL1
attL1 recombination site
570
665
recombination site
attL2
attL2 recombination site
793
696
selectable marker
SpnR
spectinomycin resistance gene
1062
2072
trxn termination sequence
T1-T2
rrnBT1 and T2 terminators
510
224
