Gene:
Gene Symbol:
PPP1R13B
Gene Name:
protein phosphatase 1, regulatory subunit 13B
Original Clone ID:
ccsbBroadEn_02755
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC136527.1
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00516477
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGATGCCGATGATATTAACTGTTTTCTTGAGCAACAATGAACAGATTTTAACAGAAGTT
CCTATAACACCGGAAACAACCTGTCGAGATGTTGTAGAATTTTGCAAGGAACCTGGAGAA
GGCAGCTGCCATTTAGCTGAAGTGTGGAGGGGAAATGAACGTCCCATACCCTTTGATCAT
ATGATGTACGAACATCTTCAGAAATGGGGTCCACGGAGGGAAGAAGTGAAATTTTTCCTT
CGACACGAGGACTCCCCAACTGAGAACAGTGAACAAGGTGGCCGTCAGACCCAAGAGCAA
CGAACTCAGAGAAATGTAATAAATGTACCTGGAGAAAAACGTACTGAAAATGGGGTTGGG
AATCCACGTGTTGAACTTACCCTCTCAGAGCTCCAAGATATGGCAGCTAGGCAACAGCAG
CAGATTGAAAATCAGCAGCAGATGTTGGTTGCCAAGGAACAGCGTTTACATTTTCTAAAG
CAACAGGAGCGCCGTCAGCAGCAGTCTATTTCTGAAAATGAAAAGCTTCAGAAATTGAAA
GAACGAGTTGAAGCCCAGGAGAACAAGCTGAAGAAAATTCGTGCAATGAGAGGACAAGTC
GACTACAGCAAAATCATGAACGGCAATCTGTCTGCTGAAATAGAAAGGTTCAGTGCCATG
TTCCAGGAAAAGAAGCAGGAAGTACAGACTGCAATTTTAAGGGTTGATCAGCTTAGTCAG
CAATTGGAAGATTTAAAGAAAGGAAAACTGAATGGGTTCCAGTCTTACAATGGCAAATTG
ACGGGACCAGCGGCGGTGGAGTTAAAAAGACTGTACCAAGAACTACAGATTCGTAACCAA
CTTAACCAGGAACAAAATTCAAAACTTCAGCAGCAGAAGGAACTCTTAAATAAGCGCAAC
ATGGAGGTGGCCATGATGGACAAGCGAATCAGTGAACTGCGTGAACGTCTCTATGGGAAA
AAAATTCAGCTGAACCGTGTGAATGGCACGTCATCACCACAGTCCCCTCTGAGCACATCG
GGCAGGGTCGCTGCTGTGGGGCCTTATATCCAGGTTCCCAGTGCCGGAAGCTTTCCTGTG
CTGGGGGACCCTATAAAGCCCCAGTCTCTCAGTATTGCCTCAAATGCTGCTCATGGAAGA
TCCAAATCCGCTAATGATGGAAACTGGCCAACATTAAAACAGAATTCTAGCTCTTCCGTG
AAACCAGTGCAGGTGGCCGGTGCAGACTGGAAGGATCCGAGCGTGGAGGGGTCTGTCAAG
CAGGGCACTGTCTCCAGCCAGCCTGTGCCCTTCTCAGCACTGGGACCCACGGAGAAGCCG
GGCATCGAGATTGGTAAAGTGCCACCTCCCATCCCGGGTGTAGGCAAGCAGCTGCCTCCA
AGCTATGGGACATACCCAAGTCCTACACCTCTGGGTCCTGGGTCGACAAGCTCCCTGGAA
AGGAGGAAGGAAGGCAGCTTGCCCAGGCCCAGTGCAGGCCTGCCAAGTCGACAGAGGCCC
ACCCTGCTGCCCGCCACAGGCAGCACCCCCCAGCCAGGCTCCTCACAACAGATTCAGCAG
AGGATTTCCGTACCGCCAAGTCCCACGTACCCGCCAGCGGGACCACCTGCATTTCCAGCT
GGGGACAGCAAGCCTGAACTCCCACTGACAGTGGCCATTAGGCCTTTCCTGGCTGATAAA
GGGTCAAGGCCACAGTCTCCCAGGAAAGGACCCCAGACAGTGAATTCAAGTTCCATATAC
TCCATGTACCTCCAGCAAGCCACACCACCTAAGAATTACCAGCCGGCAGCACACAGCGCC
TTAAATAAGTCAGTTAAAGCAGTGTATGGTAAGCCCGTTTTACCTTCGGGTTCAACCTCT
CCATCGCCGCTGCCGTTTCTTCACGGGTCACTGTCCACGGGCACACCACAGCCTCAGCCA
CCTTCAGAAAGTACTGAGAAAGAGCCTGAGCAGGATGGCCCCGCCGCCCCCGCAGATGGC
AGCACCGTGGAGAGCCTGCCACGGCCACTCAGCCCCACCAAGCTCACGCCCATCGTGCAT
TCGCCACTGCGCTACCAGAGTGATGCAGACCTGGAGGCCCTCCGCAGGAAGCTGGCCAAC
GCGCCCCGGCCCCTGAAAAAGCGCAGCTCCATCACAGAGCCCGAGGGCCCCGGCGGGCCC
AACATCCAGAAGCTGCTGTACCAGCGCTTCAACACCCTGGCCGGTGGCATGGAGGGCACC
CCTTTCTACCAGCCCAGCCCCTCCCAGGACTTCATGGGCACCTTGGCCGATGTGGACAAT
GGAAACACCAATGCCAATGGAAACCTGGAAGAGCTCCCCCCTGCCCAGCCCACAGCCCCA
CTCCCCGCTGAGCCTGCCCCGTCATCAGATGCCAATGATAATGAGTTACCTTCCCCCGAA
CCAGAGGAGCTCATCTGTCCCCAAACCACCCACCAAACTGCCGAGCCGGCAGAGGACAAT
AACAACAACGTGGCCACGGTCCCCACCACGGAGCAGATCCCGAGTCCTGTGGCTGAGGCC
CCATCTCCAGGGGAAGAGCAGGTCCCTCCAGCACCTCTTCCCCCTGCCAGCCACCCTCCT
GCCACCTCCACGAACAAGCGGACCAACTTGAAGAAGCCCAACTCGGAGCGGACGGGGCAC
GGGCTGAGAGTCCGGTTTAACCCCCTGGCACTGCTCCTAGACGCGTCTCTGGAAGGAGAG
TTCGATCTGGTGCAGAGGATCATCTATGAGGTGGAAGATCCCAGCAAGCCCAACGACGAA
GGGATCACCCCACTGCACAACGCCGTCTGCGCCGGCCACCATCACATCGTGAAGTTCCTG
CTGGATTTTGGTGTCAACGTGAATGCTGCTGATAGTGATGGATGGACGCCGCTGCACTGC
GCTGCCTCTTGTAACAGCGTTCACCTCTGCAAACAGCTGGTGGAGAGTGGTGCCGCCATT
TTTGCCTCAACCATAAGCGACATTGAAACTGCTGCAGACAAGTGTGAGGAGATGGAGGAA
GGCTACATCCAGTGCTCCCAGTTTCTATATGGGGTGCAGGAAAAGCTGGGTGTGATGAAC
AAAGGTGTGGCGTATGCTCTGTGGGACTACGAGGCCCAGAACAGTGACGAGCTGTCCTTC
CACGAAGGGGACGCCCTCACCATCCTGAGGCGCAAGGACGAAAGCGAGACTGAGTGGTGG
TGGGCTCGCCTTGGAGACCGGGAGGGCTATGTGCCCAAAAACCTGCTGGGGCTGTATCCA
CGGATCAAACCCCGACAGCGAACACTCGCC
Protein sequence predicted based on nucleotide sequence
MMPMILTVFLSNNEQILTEVPITPETTCRDVVEFCKEPGEGSCHLAEVWRGNERPIPFDH MMYEHLQKWGPRREEVKFFLRHEDSPTENSEQGGRQTQEQRTQRNVINVPGEKRTENGVG NPRVELTLSELQDMAARQQQQIENQQQMLVAKEQRLHFLKQQERRQQQSISENEKLQKLK ERVEAQENKLKKIRAMRGQVDYSKIMNGNLSAEIERFSAMFQEKKQEVQTAILRVDQLSQ QLEDLKKGKLNGFQSYNGKLTGPAAVELKRLYQELQIRNQLNQEQNSKLQQQKELLNKRN MEVAMMDKRISELRERLYGKKIQLNRVNGTSSPQSPLSTSGRVAAVGPYIQVPSAGSFPV LGDPIKPQSLSIASNAAHGRSKSANDGNWPTLKQNSSSSVKPVQVAGADWKDPSVEGSVK QGTVSSQPVPFSALGPTEKPGIEIGKVPPPIPGVGKQLPPSYGTYPSPTPLGPGSTSSLE RRKEGSLPRPSAGLPSRQRPTLLPATGSTPQPGSSQQIQQRISVPPSPTYPPAGPPAFPA GDSKPELPLTVAIRPFLADKGSRPQSPRKGPQTVNSSSIYSMYLQQATPPKNYQPAAHSA LNKSVKAVYGKPVLPSGSTSPSPLPFLHGSLSTGTPQPQPPSESTEKEPEQDGPAAPADG STVESLPRPLSPTKLTPIVHSPLRYQSDADLEALRRKLANAPRPLKKRSSITEPEGPGGP NIQKLLYQRFNTLAGGMEGTPFYQPSPSQDFMGTLADVDNGNTNANGNLEELPPAQPTAP LPAEPAPSSDANDNELPSPEPEELICPQTTHQTAEPAEDNNNNVATVPTTEQIPSPVAEA PSPGEEQVPPAPLPPASHPPATSTNKRTNLKKPNSERTGHGLRVRFNPLALLLDASLEGE FDLVQRIIYEVEDPSKPNDEGITPLHNAVCAGHHHIVKFLLDFGVNVNAADSDGWTPLHC AASCNSVHLCKQLVESGAAIFASTISDIETAADKCEEMEEGYIQCSQFLYGVQEKLGVMN KGVAYALWDYEAQNSDELSFHEGDALTILRRKDESETEWWWARLGDREGYVPKNLLGLYP RIKPRQRTLA
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223
Synonyms:
pDONR223, pENTR223-XnCDSYn
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp):
2815
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Mark Vidal
Publications:
PMID:
15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
bacterial origin of replication
2220
2753
gene fragment
pas
5p stem of pBR primose assembly site
63
73
gene fragment
pas prime
3p stem of pBR pas(L)
105
116
recombination site
attL1
attL1 recombination site
570
665
recombination site
attL2
attL2 recombination site
793
696
selectable marker
SpnR
spectinomycin resistance gene
1062
2072
trxn termination sequence
T1-T2
rrnBT1 and T2 terminators
510
224
