Gene:
Gene Symbol:
CEP135
Gene Name:
centrosomal protein 135kDa
Original Clone ID:
ccsbBroadEn_02222
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC136535.1
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00516485
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGACTACAGCTGTAGAGAGAAAGTATATTAATATTAGGAAAAGGCTGGATCAGCTGGGA
TACCGCCAGACTCTGACAGTGGAGTGTTTACCTTTGGTAGAAAAACTTTTCAGCGACTTA
GTTCATACAACTGAGAGCCTTCGGCAATCAAAATTATCTGCTGTGAAAGCTGAAAAAGAA
AGTGCCAATTTTGATTTTGTTTTGGAACCCTATAAACTTGAAAATGCAAGATTGAGTAGA
GAAAATAATGAATTATACCTAGAGTTAATGAAACTGAGAGAACATTCAGACCAACACGTT
AAAGAGTTGAAAACTTCATTGAAGAAATGTGCACGTGAAACAGCTGATCTGAAATTTCTG
AATAACCAATATGCTCATAAACTCAAACTGTTGGAGAAAGAGAGCAAAGCTAAGAATGAA
AGAATTCAACAACTTCAAGAAAAGAATTTGCATGCTGTAGTACAAACTCCAGGTGGCAAG
AAAAGAAGTATTGCTTTCAGGCGCCAGCGTATGCAAATTGATGAACCGGTTCCTCCCTCT
GAAGTCAGTTCATATCCAGTTCCTCAACCAGATGACCCTTACATTGCAGACCTCCTTCAA
GTGGCTGATAACAGGATTCAAGAACTTCAACAGGAAGTCCACCAGCTACAAGAAAAGTTA
GCAATGATGGAAAGTGGGGTGAGAGACTATAGCAAGCAGATTGAGCTAAGAGAACGAGAG
ATAGAACGACTGTCAGTTGCTTTGGATGGTGGTCGGTCCCCTGATGTCCTTTCTCTGGAG
TCTAGAAATAAAACCAATGAAAAGCTTATTGCTCATTTAAATATTCAGGTTGACTTTCTT
CAGCAAGCTAATAAAGACCTGGAGAAGCGTATACGAGAGCTTATGGAAACCAAGGAAACA
GTGACATCTGAAGTCGTTAATTTAAGTAACAAAAATGAAAAACTCTGCCAAGAATTAACT
GAAATAGATCAGTTAGCACAGCAGTTGGAAAGACATAAAGAAGAAGTGCTTGAGACTGCT
GATAAAGAGCTTGGGGAAGCAAAGAAAGAGATTAAAAGAAAGCTCTCTGAAATGCAGGAT
CTTGAAGAAACAATGGCAAAACTTCAGCTGGAATTGAACTTATGCCAGAAAGAAAAGGAG
AGACTGAGTGATGAACTCCTTGTAAAATCAGACCTAGAAACTGTTGTTCATCAGCTTGAA
CAAGAAAAGCAAAGACTTAGCAAAAAAGTTGAAAGTTTTGCAGTTACAGAACGACAACTT
ACTCTGGAGGTTGAGAGGATGAGACTAGAACATGGAATAAAACGTCGAGACAGGTCACCT
TCTCGTTTAGATACATTTCTGAAAGGTATAGAAGAAGAACGAGATTATTATAAGAAAGAG
CTAGAGAGACTCCAACATATAATACAGCGAAGATCTTGCTCTACAAGTTATAGCGCACGT
GAAAAAAGTTCAATATTTAGAACACCAGAAAAGGGTGATTACAATTCAGAAATTCATCAG
ATCACAAGAGAAAGAGATGAACTTCAGCGTATGCTAGAAAGATTTGAAAAATATATGGAA
GATATACAGTCCAATGTTAAATTATTGACAGCAGAAAGAGATAAACTAAGTGTCTTATAT
AATGAAGCTCAGGAAGAATTATCTGCCCTAAGAAAGGAATCCACCCAAACCACAGCACCC
CATAATATTGTTAGTCTTATGGAAAAGGAAAAAGAACTTGCGTTATCTGACTTAAGAAGA
ATTATGGCAGAAAAGGAAGCTTTAAGAGAAAAATTAGAGCATATTGAAGAAGTGAGTCTT
TTTGGAAAATCAGAATTAGAGAAAACTATTGAACATTTGACATGTGTTAATCATCAGCTT
GAAAGCGAAAAATATGAATTAAAGTCTAAAGTGTTAATAATGAAAGAAACAATAGAGTCG
TTAGAGAACAAATTAAAAGTCCAAGCTCAAAAATTTAGCCATGTGGCTGGTGACTCATCT
CATCAGAAAACAGAGGTGAACTCACTTAGGATAGTGAATGAGCAGCTACAGCGGTCAGTT
GATGACTATCAGCACCGACTTTCCATAAAAAGAGGTGAACTTGAATCAGCCCAAGCACAA
ATTAAAATACTGGAGGAAAAGATAGATGAACTAAACCTTAAGATGACTTCACAGGATGAG
GAGGCTCATGTAATGAAAAAGACCATTGGTGTTATTGATAAAGAAAAAGACTTTCTCCAG
GAGACTGTAGATGAGAAGACAGAAAAGATTGCAAATTTGCAAGAAAACCTAGCTAATAAA
GAAAAAGCTGTTGCTCAAATGAAGATAATGATCTCAGAGTGTGAATCATCTGTGAACCAG
CTGAAAGAAACATTGGTTAATCGAGATCGTGAGATAAACAGCCTCCGGCGCCAGCTTGAT
GCAGCTCACAAAGAACTCGATGAAGTAGGAAGATCTAGAGAAATCGCTTTTAAGGAAAAC
AGAAGACTGCAAGATGACCTGGCTACAATGGCAAGAGAAAATCAAGAAATCTCATTGGAA
TTGGAAGCAGCAGTGCAAGAAAAAGAAGAAATGAAGAGCAGAGTTCATAAATACATAACA
GAGGTGTCACGATGGGAGAGCTTAATGGCTGCCAAGGAAAAAGAAAATCAAGATTTGTTA
GATAGATTTCAGATGCTTCATAACCGTGCTGAAGACTGGGAGGTCAAAGCCCATCAAGCT
GAGGGAGAAAGCAGCTCAGTTCGACTGGAACTTCTTTCTATTGACACTGAGAGGAGACAT
CTTCGAGAAAGAGTGGAGCTATTAGAAAAAGAAATTCAAGAGCACATAAATGCCCATCAT
GCTTATGAATCTCAGATCTCATCAATGGCAAAAGCCATGTCTCGATTAGAAGAAGAGCTG
AGACATCAAGAAGATGAGAAAGCAACAGTATTAAATGACTTGTCATCTCTTAGAGAACTT
TGCATTAAACTTGATTCAGGCAAAGATATTATGACCCAGCAATTGAATTCGAAAAACCTT
GAGTTTGAGAGGGTTGTGGTGGAATTAGAAAATGTAAAGTCAGAGTCAGACCTACTGAAA
AAACAACTTTCAAATGAGAGACATACAGTTAAAAACCTCGAATCATTGTTGGCTACAAAC
AGAGATAAAGAATTTCATTCTCACTTAACCTCCCACGAGAAGGATACAGAAATCCAGCTA
CTTAAGGAGAAGTTAACCCTTTCTGAAAGCAAATTAACTAGTCAAAGCCGGGAAAACACC
ATGCTTCGAGCTAAAGTGGCACAGTTACAAACAGATTATGATGCTCTGAAAAGGCAGATC
TCAACTGAAAGATACGAACGAGAACGAGCAATCCAAGAGATGCGTCGACATGGTCTTGCT
ACACCACCCCTTAGTTCCACTCTGAGGTCTCCTTCACATTCTCCTGAACATAGAAATGTG
Protein sequence predicted based on nucleotide sequence
MTTAVERKYINIRKRLDQLGYRQTLTVECLPLVEKLFSDLVHTTESLRQSKLSAVKAEKE SANFDFVLEPYKLENARLSRENNELYLELMKLREHSDQHVKELKTSLKKCARETADLKFL NNQYAHKLKLLEKESKAKNERIQQLQEKNLHAVVQTPGGKKRSIAFRRQRMQIDEPVPPS EVSSYPVPQPDDPYIADLLQVADNRIQELQQEVHQLQEKLAMMESGVRDYSKQIELRERE IERLSVALDGGRSPDVLSLESRNKTNEKLIAHLNIQVDFLQQANKDLEKRIRELMETKET VTSEVVNLSNKNEKLCQELTEIDQLAQQLERHKEEVLETADKELGEAKKEIKRKLSEMQD LEETMAKLQLELNLCQKEKERLSDELLVKSDLETVVHQLEQEKQRLSKKVESFAVTERQL TLEVERMRLEHGIKRRDRSPSRLDTFLKGIEEERDYYKKELERLQHIIQRRSCSTSYSAR EKSSIFRTPEKGDYNSEIHQITRERDELQRMLERFEKYMEDIQSNVKLLTAERDKLSVLY NEAQEELSALRKESTQTTAPHNIVSLMEKEKELALSDLRRIMAEKEALREKLEHIEEVSL FGKSELEKTIEHLTCVNHQLESEKYELKSKVLIMKETIESLENKLKVQAQKFSHVAGDSS HQKTEVNSLRIVNEQLQRSVDDYQHRLSIKRGELESAQAQIKILEEKIDELNLKMTSQDE EAHVMKKTIGVIDKEKDFLQETVDEKTEKIANLQENLANKEKAVAQMKIMISECESSVNQ LKETLVNRDREINSLRRQLDAAHKELDEVGRSREIAFKENRRLQDDLATMARENQEISLE LEAAVQEKEEMKSRVHKYITEVSRWESLMAAKEKENQDLLDRFQMLHNRAEDWEVKAHQA EGESSSVRLELLSIDTERRHLRERVELLEKEIQEHINAHHAYESQISSMAKAMSRLEEEL RHQEDEKATVLNDLSSLRELCIKLDSGKDIMTQQLNSKNLEFERVVVELENVKSESDLLK KQLSNERHTVKNLESLLATNRDKEFHSHLTSHEKDTEIQLLKEKLTLSESKLTSQSRENT MLRAKVAQLQTDYDALKRQISTERYERERAIQEMRRHGLATPPLSSTLRSPSHSPEHRNV
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223
Synonyms:
pDONR223, pENTR223-XnCDSYn
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp):
2815
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Mark Vidal
Publications:
PMID:
15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
bacterial origin of replication
2220
2753
gene fragment
pas
5p stem of pBR primose assembly site
63
73
gene fragment
pas prime
3p stem of pBR pas(L)
105
116
recombination site
attL1
attL1 recombination site
570
665
recombination site
attL2
attL2 recombination site
793
696
selectable marker
SpnR
spectinomycin resistance gene
1062
2072
trxn termination sequence
T1-T2
rrnBT1 and T2 terminators
510
224
