Gene:
Gene Symbol:
KANK1
Gene Name:
KN motif and ankyrin repeat domains 1
Original Clone ID:
ccsbBroadEn_07849
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC037495.2
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00516496
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGCTCACACCACAAAGGTTAACGGCAGTGCCTCAGGAAAAGCAGGTGATATTCTCAGT
GGAGACCAGGACAAGGAACAGAAAGACCCTTACTTTGTGGAGACCCCCTATGGTTATCAA
CTAGACTTAGATTTCCTCAAATATGTGGATGACATACAGAAGGGAAATACCATCAAAAGA
CTGAACATCCAGAAGAGGCGGAAGCCGTCCGTGCCATGCCCAGAACCCAGGACCACATCT
GGTCAGCAAGGTATATGGACTTCCACTGAATCCCTCTCATCCTCCAACAGTGATGACAAC
AAGCAGTGCCCCAACTTCCTCATAGCCAGAAGTCAAGTTACATCAACTCCAATCTCAAAG
CCACCTCCCCCTCTGGAGACCTCACTCCCTTTTCTTACCATCCCAGAAAATCGACAGCTG
CCACCTCCCTCACCACAACTCCCAAAGCATAACCTTCATGTCACCAAGACACTGATGGAG
ACCCGGAGAAGACTGGAACAGGAGAGAGCCACCATGCAGATGACACCGGGTGAGTTCAGA
AGGCCCAGGCTGGCCAGTTTTGGAGGCATGGGCACCACAAGCTCCCTCCCTTCTTTTGTG
GGTTCTGGAAACCACAAGCCTGCCAAGCACCAGCTTCAGAATGGATACCAAGGTAATGGG
GATTATGGTAGCTATGCCCCAGCTGCTCCCACCACTTCCTCCATGGGGAGCTCCATCCGC
CACAGCCCCCTGAGCTCAGGGATCTCCACCCCAGTGACCAACGTGAGCCCCATGCACCTG
CAGCACATCCGCGAGCAGATGGCCATTGCTCTGAAACGCCTGAAGGAGCTGGAGGAGCAG
GTGCGAACCATCCCTGTGCTCCAGGTAAAGATCTCTGTCTTGCAAGAAGAGAAAAGGCAG
TTGGTCTCACAGCTGAAAAACCAAAGGGCTGCATCCCAGATCAATGTCTGTGGTGTGAGG
AAGCGGTCCTATAGTGCGGGGAACGCCTCCCAGCTGGAACAGCTCTCCCGGGCCCGAAGA
AGTGGCGGGGAATTATACATTGACTATGAGGAGGAAGAAATGGAGACCGTAGAACAGAGC
ACGCAGAGGATAAAGGAGTTCCGGCAACTTACAGCAGACATGCAAGCCCTGGAGCAGAAG
ATCCAGGACAGCAGCTGTGAGGCCTCCTCAGAGCTCAGGGAGAATGGAGAGTGCCGGTCT
GTGGCTGTGGGTGCCGAGGAGAACATGAACGACATCGTCGTGTACCACAGAGGCTCCAGG
TCCTGTAAGGATGCAGCTGTAGGGACACTTGTTCAGATGAGAAATTGTGGGGTCAGCGTG
ACAGAGGCCATGCTTGGAGTGATGACTGAAGCTGACAAAGAAATTGAGCTGCAACAGCAG
ACCATAGAATCCTTGAAGGAAAAGATCTATCGCCTAGAAGTACAGCTTAGAGAAACCACC
CATGACCGGGAGATGACTAAACTGAAACAAGAGCTGCAGGCTGCTGGATCGAGGAAAAAG
GTTGACAAAGCCACGATGGCCCAGCCGCTTGTTTTCAGTAAGGTGGTGGAGGCAGTGGTG
CAGACCAGAGACCAAATGGTCGGCAGTCACATGGACCTGGTGGACACGTGTGTTGGGACC
TCCGTGGAAACAAACAGTGTAGGCATCTCCTGCCAGCCTGAATGTAAGAATAAAGTCGTA
GGGCCTGAGCTGCCTATGAATTGGTGGATTGTTAAGGAGAGGGTGGAAATGCATGACCGA
TGTGCTGGGAGGTCTGTGGAAATGTGTGACAAGAGTGTGAGTGTGGAAGTCAGCGTCTGC
GAAACAGGCAGCAACACAGAGGAGTCTGTGAATGACCTCACACTCCTCAAGACAAACTTG
AATCTCAAAGAAGTGCGGTCTATCGGTTGTGGAGATTGTTCTGTTGACGTGACCGTCTGC
TCTCCAAAGGAGTGCGCCTCCCGGGGCGTGAACACTGAGGCTGTTAGCCAGGTGGAAGCT
GCCGTCATGGCAGTGCCTCGTACTGCAGACCAGGACACTAGCACAGATTTGGAACAGGTG
CACCAGTTCACCAACACCGAGACGGCCACCCTCATAGAGTCCTGCACCAACACTTGTCTA
AGCACTTTGGACAAGCAGACCAGCACCCAGACTGTGGAGACGCGGACAGTAGCTGTAGGA
GAAGGCCGTGTCAAGGACATCAACTCCTCCACCAAGACGCGGTCCATTGGTGTTGGAACG
TTGCTTTCTGGCCATTCTGGGTTTGACAGGCCATCAGCTGTGAAGACCAAAGAGTCAGGT
GTGGGGCAGATAAATATTAACGACAACTATCTGGTTGGTCTCAAAATGAGGACTATAGCT
TGTGGGCCACCACAGTTGACTGTGGGGCTGACAGCCAGCAGAAGGAGCGTGGGGGTTGGG
GATGACCCTGTAGGGGAATCTCTGGAGAACCCCCAGCCTCAAGCTCCACTTGGAATGATG
ACTGGCCTGGATCACTACATTGAGCGTATCCAGAAGCTGCTGGCAGAACAGCAGACACTG
CTGGCTGAGAACTACAGTGAACTGGCAGAAGCTTTCGGGGAACCTCACTCACAGATGGGC
TCCCTCAACTCTCAGCTCATCAGCACCCTGTCGTCTATCAACTCTGTCATGAAATCTGCA
AGCACTGAAGAGCTGAGGAACCCTGACTTCCAGAAAACCAGTCTGGGTAAAATCACAGGC
AATTATTTGGGATATACCTGTAAGTGTGGGGGCCTTCAGTCAGGAAGTCCCTTAAGCTCC
CAGACATCCCAGCCTGAGCAAGAAGTGGGGACCTCAGAAGGAAAGCCAATCAGCAGCCTG
GATGCCTTCCCCACTCAGGAAGGTACGCTGTCTCCAGTGAACCTGACAGACGACCAGATC
GCCGCTGGCCTCTATGCATGTACAAACAATGAAAGTACACTGAAGTCCATCATGAAGAAG
AAAGATGGTAACAAAGATTCAAATGGCGCAAAAAAGAATCTTCAGTTTGTTGGCATTAAT
GGAGGGTATGAAACAACTTCAAGTGATGATTCCAGCTCAGATGAAAGCTCTTCTTCCGAG
TCAGATGACGAGTGTGATGTCATTGAGTATCCTCTTGAAGAAGAGGAGGAGGAGGAGGAT
GAAGACACTCGGGGAATGGCAGAAGGGCACCATGCAGTTAATATTGAAGGTTTGAAGTCT
GCCAGGGTGGAAGATGAAATGCAGGTTCAAGAATGTGAACCTGAGAAGGTGGAAATCAGA
GAGAGGTATGAATTAAGTGAAAAGATGTTGTCTGCATGCAACTTACTGAAAAATACTATA
AATGACCCCAAAGCTTTGACCAGCAAAGATATGAGGTTCTGTCTGAACACCCTCCAGCAC
GAGTGGTTCCGCGTGTCCAGTCAGAAGTCAGCCATTCCAGCCATGGTGGGGGACTACATA
GCTGCTTTTGAGGCCATTTCCCCAGATGTCCTCCGCTATGTCATCAACTTGGCAGACGGC
AACGGCAACACAGCCCTCCATTACAGCGTGTCCCACTCCAACTTCGAGATTGTGAAGCTG
CTGTTAGATGCCGATGTGTGTAATGTGGATCACCAGAACAAGGCAGGCTACACCCCCATC
ATGTTGGCGGCCCTCGCCGCTGTGGAAGCAGAGAAGGACATGCGGATTGTGGAAGAACTC
TTTGGCTGTGGGGATGTGAATGCCAAAGCTAGTCAGGCGGGACAGACGGCCCTCATGCTG
GCGGTCAGTCACGGACGGATAGACATGGTGAAGGGCCTTCTGGCCTGTGGGGCTGATGTC
AACATCCAGGATGACGAGGGCTCCACGGCCCTCATGTGTGCCAGCGAGCACGGGCACGTG
GAGATTGTCAAGCTGCTGCTGGCCCAGCCCGGCTGCAACGGTCACCTAGAGGACAACGAT
GGCAGCACTGCGCTCTCAATCGCCCTGGAAGCAGGACACAAGGACATCGCTGTTCTTCTG
TATGCCCATGTCAACTTTGCAAAAGCCCAGTCTCCGGGCACCCCTAGGCTTGGAAGGAAG
ACGTCTCCTGGCCCCACCCACCGAGGTTCATTTGAT
Protein sequence predicted based on nucleotide sequence
MAHTTKVNGSASGKAGDILSGDQDKEQKDPYFVETPYGYQLDLDFLKYVDDIQKGNTIKR LNIQKRRKPSVPCPEPRTTSGQQGIWTSTESLSSSNSDDNKQCPNFLIARSQVTSTPISK PPPPLETSLPFLTIPENRQLPPPSPQLPKHNLHVTKTLMETRRRLEQERATMQMTPGEFR RPRLASFGGMGTTSSLPSFVGSGNHKPAKHQLQNGYQGNGDYGSYAPAAPTTSSMGSSIR HSPLSSGISTPVTNVSPMHLQHIREQMAIALKRLKELEEQVRTIPVLQVKISVLQEEKRQ LVSQLKNQRAASQINVCGVRKRSYSAGNASQLEQLSRARRSGGELYIDYEEEEMETVEQS TQRIKEFRQLTADMQALEQKIQDSSCEASSELRENGECRSVAVGAEENMNDIVVYHRGSR SCKDAAVGTLVQMRNCGVSVTEAMLGVMTEADKEIELQQQTIESLKEKIYRLEVQLRETT HDREMTKLKQELQAAGSRKKVDKATMAQPLVFSKVVEAVVQTRDQMVGSHMDLVDTCVGT SVETNSVGISCQPECKNKVVGPELPMNWWIVKERVEMHDRCAGRSVEMCDKSVSVEVSVC ETGSNTEESVNDLTLLKTNLNLKEVRSIGCGDCSVDVTVCSPKECASRGVNTEAVSQVEA AVMAVPRTADQDTSTDLEQVHQFTNTETATLIESCTNTCLSTLDKQTSTQTVETRTVAVG EGRVKDINSSTKTRSIGVGTLLSGHSGFDRPSAVKTKESGVGQININDNYLVGLKMRTIA CGPPQLTVGLTASRRSVGVGDDPVGESLENPQPQAPLGMMTGLDHYIERIQKLLAEQQTL LAENYSELAEAFGEPHSQMGSLNSQLISTLSSINSVMKSASTEELRNPDFQKTSLGKITG NYLGYTCKCGGLQSGSPLSSQTSQPEQEVGTSEGKPISSLDAFPTQEGTLSPVNLTDDQI AAGLYACTNNESTLKSIMKKKDGNKDSNGAKKNLQFVGINGGYETTSSDDSSSDESSSSE SDDECDVIEYPLEEEEEEEDEDTRGMAEGHHAVNIEGLKSARVEDEMQVQECEPEKVEIR ERYELSEKMLSACNLLKNTINDPKALTSKDMRFCLNTLQHEWFRVSSQKSAIPAMVGDYI AAFEAISPDVLRYVINLADGNGNTALHYSVSHSNFEIVKLLLDADVCNVDHQNKAGYTPI MLAALAAVEAEKDMRIVEELFGCGDVNAKASQAGQTALMLAVSHGRIDMVKGLLACGADV NIQDDEGSTALMCASEHGHVEIVKLLLAQPGCNGHLEDNDGSTALSIALEAGHKDIAVLL YAHVNFAKAQSPGTPRLGRKTSPGPTHRGSFD
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223
Synonyms:
pDONR223, pENTR223-XnCDSYn
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp):
2815
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Mark Vidal
Publications:
PMID:
15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
bacterial origin of replication
2220
2753
gene fragment
pas
5p stem of pBR primose assembly site
63
73
gene fragment
pas prime
3p stem of pBR pas(L)
105
116
recombination site
attL1
attL1 recombination site
570
665
recombination site
attL2
attL2 recombination site
793
696
selectable marker
SpnR
spectinomycin resistance gene
1062
2072
trxn termination sequence
T1-T2
rrnBT1 and T2 terminators
510
224
