Gene:
Gene Symbol:
ANKRD26
Gene Name:
ankyrin repeat domain 26
Original Clone ID:
ccsbBroadEn_11635
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC130453.1
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00516504
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 5178nts
Open reading frame: 1 to 5178
ATGAAGAAGATTTTTAGTAAGAAGGGCGAGTCGCCCTTGGGCTCCTTCGCGCGGCGGCAG
AGGAGCAGCGCGGGAGGCGGGGGCGAGCCGGGGGAGGGCGCCTACTCGCAGCCCGGCTAC
CACGTCCGAGACCGAGATCTCGGCAAGATCCACAAAGCTGCCAGCGCGGGTAATGTGGCG
AAAGTGCAGCAGATCCTTTTGCTCAGGAAGAATGGCTTGAACGATAGAGACAAGATGAAC
AGGACGGCTCTACATTTGGCCTGTGCCAATGGTCATCCAGAAGTAGTAACTCTCCTGGTG
GACAGAAAATGCCAGCTCAATGTCTGTGACAACGAAAACAGGACAGCTCTGATGAAGGCT
GTACAATGCCAGGAAGAGAAATGTGCAACTATTCTGCTAGAACATGGTGCTGATCCAAAT
CTTGCGGATGTCCATGGCAACACTGCTCTTCACTATGCTGTCTATAATGAGGACATATCA
GTAGCAACAAAGCTGCTTTTGTATGATGCAAATATTGAAGCAAAAAACAAGGATGACCTC
ACACCACTTTTACTTGCAGTAAGTGGAAAAAAGCAGCAAATGGTGGAATTTTTAATAAAG
AAAAAAGCAAATGTAAATGCAGTAGATAAGTTGGAAAGCAGTCACCAACTAATTTCAGAA
TATAAAGAAGAAAGGATACCTAAACATTCTTCTCAAAATAGTAATTCAGTGGATGAAAGC
TCTGAAGACTCCTTAAGCAGTTTATTTTATTTTATTTTTTTTGAGGCAAAGTCTTGCACT
GTTGCCCAGGCTGGAGTGCTACAGCATGATCTCGGCTCACTGCAATCTCTGCTTCCTGGG
TTCACACAATTCTTCTGCCTCCACCTCCCAAGTAGCTGGGACCACAGGCTTTCTGGCAAA
CCGGGTGTTGATGATTCATGGCCTACCTCAGATGACGAAGACCTCAATTTTGATACTAAG
AATGTCCCAAAACCAAGCTTAGCAAAGCTAATGACTGCTTCTCAGCAATCCAGGAAAAAT
TTAGAAGCAACATATGGCACTGTGAGAACAGGAAATAGAACTTTGTTTGAGGATAGAGAT
TCCGATAGTCAAGATGAAGTTGTGGTTGAAAGCCTTCCTACAACATCAATCAAAGTCCAG
TGCTTTTCTCATCCTACCTATCAATCACCTGACCTTCTTCCAAAACCTTCCCACAAGTCG
TTAGCAAACCCTGGTCTTATGAAGGAAGAACCAACAAAGCCAGGCATTGCAAAAAAAGAA
AATGGTATTGATATTATTGAAAGTGCTCCACTAGAGCAAACAAATAATGACAATTTGACT
TATGTTGATGAAGTGCACAAAAATAATAGAAGTGATATGATGTCCGCATTAGGATTAGGA
CAAGAGGAAGATATAGAATCACCTTGGGATTCTGAGAGTATCTCTGAGAATTTTCCACAG
AAGTATGTTGATCCTTTAGCTGGGGCTGCAGACGGAAAAGAAAAAAATATAGGAAATGAA
CAAGCAGAAGAGTCTCCTGAGAGATATCTTCACTTGAAGCCTACCATTGAAATGAAAGAT
TCTGTTCCAAATAAAGCAGGAGGAATGAAGGATGTACAAACATCCAAAGCAGCTGAACAT
GACTTAGAAGTAGCATCAGAAGAAGAGCAAGAAAGGGAAGGGAGTGAAAATAACCAGCCA
CAGGTTGAAGAAGAAAGGAAAAAACACAGAAATAATGAAATGGAAGTATCAGCAAACATA
CATGATGGTGCTACTGATGATGCTGAAGATGATGATGATGATGATGGATTAATTCAAAAA
AGAAAGAGTGGAGAAACTGATCATCAGCAATTTCCCAGGAAGGAAAATAAAGAGTATGCT
AGTAGTGGTCCTGCCTTGCAAATGAAGGAAGTAAAGAGCACTGAAAAAGAAAAACGGACC
TCGAAAGAATCTGTGAATTCACCAGTGTTTGGGAAGGCCAGTTTACTAACTGGTGGCCTG
CTACAAGTGGATGATGACAGCAGTTTAAGTGAAATAGATGAGGATGAAGGAAGGCCTACT
AAGAAAACATCTAATGAAAAGAACAAGGTCAAAAACCAAATACAGTCTATGGATGATGTT
GATGACTTAACTCAGTCATCTGAAACAGCCTCAGAGGATTGTGAGCTACCCCACTCTAGT
TACAAGAATTTTATGTTGCTCATTGAACAACTTGGAATGGAGTGTAAAGATTCTGTTAGC
CTATTGAAAATCCAGGATGCAGCTCTTTCATGTGAAAGATTATTAGAACTTAAAAAAAAT
CACTGTGAACTACTTACAGTAAAAATTAAAAAAATGGAAGACAAGGTTAATGTACTACAA
AGGGAGCTATCTGAAACAAAAGAAATAAAATCACAGTTAGAGCATCAAAAAGTTGAATGG
GAACGAGAACTGTGCTCTTTGAGATTTAGCTTAAACCAAGAAGAAGAGAAGAGAAGAAAT
GCTGATACGTTGTATGAAAAAATTAGGGAACAGTTAAGAAGAAAAGAAGAGCAATATAGG
AAAGAAGTTGAAGTGAAACAACAGCTTGAACTGAGTCTCCAAACACTGGAGATGGAATTG
AGGACTGTAAAAAGTAATTTGAATCAGGTCGTTCAAGAGCGAAATGACGCTCAGAGGCAA
CTTTCTCGAGAACAGAATGCCAGAATGTTACAAGATGGAATTCTGACCAATCACCTTTCC
AAACAAAAGGAGATTGAAATGGCTCAAAAGAAAATGAATTCTGAGAATTCTCATAGTCAT
GAAGAAGAAAAAGACCTATCGCATAAAAATAGCATGTTGCAGGAAGAAATTGCTATGCTA
AGACTAGAAATAGACACAATAAAAAATCAAAACCAGGAAAAAGAAAAGAAATGTTTTGAG
GACCTTAAAATTGTAAAAGAAAAGAATGAAGACCTTCAGAAGACTATAAAACAGAATGAG
GAAACATTAACACAAACAATATCCCAGTATAATGGACGGCTTAGTGTTCTGACAGCTGAG
AATGCAATGCTAAATTCTAAACTGGAGAATGAAAAGCAAAGCAAGGAAAGACTGGAAGCA
GAAGTTGAATCATACCATTCTAGATTGGCTGCTGCTATACATGATCGTGATCAAAGTGAG
ACATCAAAAAGAGAACTAGAACTTGCTTTCCAGAGAGCAAGAGATGAATGTTCTCGTTTA
CAGGACAAAATGAATTTTGATGTGTCTAACCTAAAAGATAACAATGAGATTCTTTCTCAA
CAACTATTTAAAACTGAAAGTAAACTCAATAGCCTAGAAATTGAGTTCCATCACACGAGA
GATGCCCTCAGAGAAAAGACTTTGGGTTTAGAACGGGTACAAAAGGACCTAAGCCAAACA
CAGTGTCAAATGAAGGAAATGGAACAAAAGTATCAAAATGAACAAGTTAAAGTGAATAAA
TACATTGGAAAGCAGGAGTCTGTAGAGGAGAGATTGTCTCAACTACAAAGTGAGAATATG
TTGCTTCGACAACAACTGGATGATGCCCACAACAAGGCTGACAATAAAGAGAAGACAGTG
ATTAATATCCAAGACCAGTTTCATGCTATTGTGCAAAAACTTCAAGCTGAGAGTGAAAAG
CAAAGTCTTCTGCTAGAAGAAAGAAATAAGGAGTTAATCAGTGAATGTAATCACTTAAAA
GAAAGACAGTATCAATATGAAAATGAAAAGGCAGAAAGAGAAGTTGTTGTGAGACAACTT
CAACAAGAACTAGCTGATACCCTAAAAAAACAATCTATGTCAGAGGCTTCACTGGAGGTT
ACGTCACGTTATCGTATTAATTTAGAAGATGAGACACAGGATTTAAAGAAGAAATTAGGT
CAAATCAGAAATCAATTGCAAGAAGCACAGGATCGACATACAGAAGCTGTCAGATGTGCT
GAGAAGATGCAAGATCACAAGCAAAAGCTTGAAAAAGATAATGCCAAGTTAAAAGTTACA
GTCAAAAAGCAAATGGACAAAATTGAGGAGCTTCAGAAAAACCTGTTAAATGCAAATTTG
TCTGAAGATGAAAAGGAACAATTAAAGAAACTTATGGAATTAAAACAGTCACTGGAATGT
AATTTGGATCAAGAAATGAAGAAAAATGTTGAATTAGAAAGAGAGATAACTGGATTTAAG
AACCTCTTAAAAATGACAAGAAAGAAGTTAAATGAATATGAAAATGGAGAATTTAGTTTC
CATGGAGATTTAAAAACTAGTCAATTTGAAATGGATATTCAGATTAATAAGCTAAAACAT
AAGATTGATGATCTTACAGCAGAACTGGAGACTGCAGGTTCAAAATGTCTACATCTGGAT
ACAAAGAACCAAATTCTTCAAGAGGAGTTGTTATCTATGAAAACAGTACAAAAGAAATGT
GAAAAACTACAGAAGAATAAAAAGAAGTTGGAACAAGAAGTGATCAACCTGAGAAGTCAT
ATAGAAAGGAATATGGTAGAACTTGGTCAAGTCAAACAGTATAAACAGGAGATTGAAGAA
AGAGCAAGACAGGAAATAGCAGAAAAATTAAAAGAAGTCAATCTATTTTTACAGGCACAA
GCAGCATCTCAAGAAAACTTAGAGCAGTTTAGAGAGAATAATTTTGCTTCAATGAAAAGT
CAGATGGAACTCAGAATTAAAGATCTGGAATCTGAACTCTCCAAAATAAAAACTTCTCAA
GAAGACTTTAATAAAACCGAACTGGAAAAATATAAGCAACTCTATCTAGAAGAATTAAAA
GTTAGAAAATCTTTGTCAAGTAAACTAACCAAAACTAATGAGAGGCTAGCAGAGGTCAAC
ACCAAACTTCTTGTGGAAAAACAGCAGAGCAGATCTTTGTTCACCACTCTCACTACCAGG
CCAGTCATGGAGCCACCTTGTGTGGGAAATCTTAATAATAGTTTAGATCTCAACAGAAAA
CTTATTCCAAGAGAAAACTTAGTGATCTCTACCTCAAATCCACGGGCTTCAAATAATAGC
ATGGAGAACTACTTGAGCAAGATGCAGCAGGAGTTGGAAAAAAATATAACTAGAGAACTC
AAAGAAGCTGCTGCTGAATTGGAATCTGGATCAATAGCTTCCCCTCTAGGGTCTACTGAT
GAGTCAAATCTAAATCAAGATCTAGTTTGGAAAGCATCAAGAGAATATGTACAGGTTTTA
AAGAAAAATTATATGATC
Protein sequence predicted based on nucleotide sequence
MKKIFSKKGESPLGSFARRQRSSAGGGGEPGEGAYSQPGYHVRDRDLGKIHKAASAGNVA KVQQILLLRKNGLNDRDKMNRTALHLACANGHPEVVTLLVDRKCQLNVCDNENRTALMKA VQCQEEKCATILLEHGADPNLADVHGNTALHYAVYNEDISVATKLLLYDANIEAKNKDDL TPLLLAVSGKKQQMVEFLIKKKANVNAVDKLESSHQLISEYKEERIPKHSSQNSNSVDES SEDSLSSLFYFIFFEAKSCTVAQAGVLQHDLGSLQSLLPGFTQFFCLHLPSSWDHRLSGK PGVDDSWPTSDDEDLNFDTKNVPKPSLAKLMTASQQSRKNLEATYGTVRTGNRTLFEDRD SDSQDEVVVESLPTTSIKVQCFSHPTYQSPDLLPKPSHKSLANPGLMKEEPTKPGIAKKE NGIDIIESAPLEQTNNDNLTYVDEVHKNNRSDMMSALGLGQEEDIESPWDSESISENFPQ KYVDPLAGAADGKEKNIGNEQAEESPERYLHLKPTIEMKDSVPNKAGGMKDVQTSKAAEH DLEVASEEEQEREGSENNQPQVEEERKKHRNNEMEVSANIHDGATDDAEDDDDDDGLIQK RKSGETDHQQFPRKENKEYASSGPALQMKEVKSTEKEKRTSKESVNSPVFGKASLLTGGL LQVDDDSSLSEIDEDEGRPTKKTSNEKNKVKNQIQSMDDVDDLTQSSETASEDCELPHSS YKNFMLLIEQLGMECKDSVSLLKIQDAALSCERLLELKKNHCELLTVKIKKMEDKVNVLQ RELSETKEIKSQLEHQKVEWERELCSLRFSLNQEEEKRRNADTLYEKIREQLRRKEEQYR KEVEVKQQLELSLQTLEMELRTVKSNLNQVVQERNDAQRQLSREQNARMLQDGILTNHLS KQKEIEMAQKKMNSENSHSHEEEKDLSHKNSMLQEEIAMLRLEIDTIKNQNQEKEKKCFE DLKIVKEKNEDLQKTIKQNEETLTQTISQYNGRLSVLTAENAMLNSKLENEKQSKERLEA EVESYHSRLAAAIHDRDQSETSKRELELAFQRARDECSRLQDKMNFDVSNLKDNNEILSQ QLFKTESKLNSLEIEFHHTRDALREKTLGLERVQKDLSQTQCQMKEMEQKYQNEQVKVNK YIGKQESVEERLSQLQSENMLLRQQLDDAHNKADNKEKTVINIQDQFHAIVQKLQAESEK QSLLLEERNKELISECNHLKERQYQYENEKAEREVVVRQLQQELADTLKKQSMSEASLEV TSRYRINLEDETQDLKKKLGQIRNQLQEAQDRHTEAVRCAEKMQDHKQKLEKDNAKLKVT VKKQMDKIEELQKNLLNANLSEDEKEQLKKLMELKQSLECNLDQEMKKNVELEREITGFK NLLKMTRKKLNEYENGEFSFHGDLKTSQFEMDIQINKLKHKIDDLTAELETAGSKCLHLD TKNQILQEELLSMKTVQKKCEKLQKNKKKLEQEVINLRSHIERNMVELGQVKQYKQEIEE RARQEIAEKLKEVNLFLQAQAASQENLEQFRENNFASMKSQMELRIKDLESELSKIKTSQ EDFNKTELEKYKQLYLEELKVRKSLSSKLTKTNERLAEVNTKLLVEKQQSRSLFTTLTTR PVMEPPCVGNLNNSLDLNRKLIPRENLVISTSNPRASNNSMENYLSKMQQELEKNITREL KEAAAELESGSIASPLGSTDESNLNQDLVWKASREYVQVLKKNYMI
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223
Synonyms:
pDONR223, pENTR223-XnCDSYn
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp):
2815
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Mark Vidal
Publications:
PMID:
15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
bacterial origin of replication
2220
2753
gene fragment
pas
5p stem of pBR primose assembly site
63
73
gene fragment
pas prime
3p stem of pBR pas(L)
105
116
recombination site
attL1
attL1 recombination site
570
665
recombination site
attL2
attL2 recombination site
793
696
selectable marker
SpnR
spectinomycin resistance gene
1062
2072
trxn termination sequence
T1-T2
rrnBT1 and T2 terminators
510
224
