Gene:
Gene Symbol:
act-3
Gene Name:
pyruvate formate-lyase activating enzyme
Original Clone ID:
AF1330_1_336
Keyword:
None
Species:
Archaeoglobus fulgidus DSM 4304
Type:
cDNA
Vector Name:
p11
Format:
FUSION
Source:
Midwest Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Midwest Center for Structural Genomics
Midwest Center for Structural Genomics
AfCD00519115
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 1002nts
Open reading frame: 42 to 1043
ATCACAGCAGCGGCAGAGAAAACTTGTATTTCCAGGGCCATATGATAGTGAGGTCGTACC
TTTACGAGAAGCTTAACGGAGATGTAAGGTGCAAAACCTGCTGGCATCGCTGCATTATCA
AGGAAGGAAAAACAGGAATCTGCAGGGTCAGGAAGAACGAAGGAGGGGAGCTGCTGGTTT
TAAACTACGGAATGGCATCCTCGATAGCTCTCGACCCAATCGAGAAAAAGCCACTCAACA
ACTTCAAGCCCGGCAGCAAGGTTTTGTCATTCGGCAGTGTGAGCTGCAACTTCCGCTGCC
TGCACTGCCAGAACTTTGAGATTGCCTTCGCCGATCTTGACTACTCCTACCTTAGAGAAA
TCGACCCGGATACGGTTTTGAGGATGTGCATTGACAGAAGGGCGGATGGGGTTGCTTGGA
CATACAACGAGCCGGCAATCTGGCATGAGTTTGCTCTGGACAGCTCAAAGCTCGTAAAGG
AGTCTGGATACTATGTGGTTTACGTCACCAACGGCTACATGACGCCAGAAGCGGTTGACC
AGTTTGAAGGTGTGCTTGATGCAGCCAATGTTGACGTCAAGGCCTTTAGCGAAGACTTTT
ACAAAAAAATATGCAAGGCTAAGCTTGAGCACGTGCTTGAGACGGTCGAGTACCTTCACC
GAAAGGGTGTTTTTGTCGAGCTGACCTACCTCATCATTCCCGGCGAAAACGACAGCAGAG
AGGAGCTGAGAGACTTCTGCAGGTGGGTGGCAGATTTATCTCCAAAAATCCCTGTCCACT
TCTCCCGCTTCCATCCAGATTACAAGATGCTCGACAAACCGCCAACGCCCGTTGAAAAGA
TTGAGGAGGCTGTAGAGATAGCCAAGAAGGAAGGAATTGAGTACGTTTACGCGGGAAACG
TCTGGGGGCATAGGTACGAGAACACCTACTGCCCCAACTGCGGGGAATTGCTGATTGAAA
GGGAGGGATTCTACGTTTACAACATCAATCTGGTGAGGGACAACCCGCCCAAGTGTCCTA
ACTGCGGCTATGCTCAGAACATAAATCCTC
Protein sequence predicted based on nucleotide sequence
MIVRSYLYEKLNGDVRCKTCWHRCIIKEGKTGICRVRKNEGGELLVLNYGMASSIALDPI EKKPLNNFKPGSKVLSFGSVSCNFRCLHCQNFEIAFADLDYSYLREIDPDTVLRMCIDRR ADGVAWTYNEPAIWHEFALDSSKLVKESGYYVVYVTNGYMTPEAVDQFEGVLDAANVDVK AFSEDFYKKICKAKLEHVLETVEYLHRKGVFVELTYLIIPGENDSREELRDFCRWVADLS PKIPVHFSRFHPDYKMLDKPPTPVEKIEEAVEIAKKEGIEYVYAGNVWGHRYENTYCPNC GELLIEREGFYVYNINLVRDNPPKCPNCGYAQNI
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Not_Tested
SolubleProtein: Protein _Soluble
ProteinPurified: Tested_Not_Purified
Find experimental protocols in AF1330_1_336
Recommended expression in:
Not Applicable
Vector Name:
p11
Synonyms:
''
Description:
Bacterial (E. coli) expression vector, adds His tag, TEV cleavage site; ampicillin resistance; restriction enzyme cloning.
Comments:
None
Size (bp):
5704
Parent Vector:
None
Properties:
bacterial expression, multiple cloning site, with tag/fusion/marker
Author Name:
Midwest Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
ColE1-type bacterial origin of replication
1164
1864
promoter
T7
T7 promoter
5230
5249
repressor binding site
LacO
LacO sequence region
5249
5271
repressor protein gene
lacI
lacI cds
3764
4843
selectable marker
AmpR
ampicillin resistance gene
407
1066
tag
His
6xHis tag
5329
5349
trxn termination sequence
T7 term
T7 termination sequence
5446
5492
