Gene:
Gene Symbol:
Aaci_2990
Gene Name:
radical SAM protein
Original Clone ID:
APC100356.103
Keyword:
None
Species:
Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446
Type:
cDNA
Vector Name:
pMCSG48
Format:
CLOSED
Source:
Midwest Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Jessica Bearden
PSI:Biology
Argonne National Laboratory
Midwest Center for Structural Genomics
PSI:Biology
Argonne National Laboratory
Midwest Center for Structural Genomics
AaCD00519753
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 1272nts
Open reading frame: 51 to 1322
CCGCGATGGCGCCGTATACACAACGAGAACCTGTACTTCCATCCAATGCCATGAAAATTC
TATTGGTCTCGGTTTTTGAAGGTGGGTTTCAACCTCAAACTATAGCAACTGCCGCAGGAG
CTTTATCTGAAATTGGCGTGGAGATTGATGCGTTAGATGTATATATAGAAGATCTGAGAT
TTAATAAAGTGTCCGATGCAAACCTTGTCGCGATTTCCGTGCCGCTATTTGACTCAATTC
AACCTGCTATCGCGCTGTCGAAAGAAATTCGTAAAATAAACCCACGCACACATGTCACTT
TCTTCGGTCAACATGCCACAATTCATGCACAAAAACTGGCTTCGACATATGGTGATTCAT
GCATCCGGGGTGACTGGGAATATCCGTTAATTAATCTTGCTCGGTATCTGATGGGCAATA
ATAGCGAATTAGTTGGGGTACTTCTTGCTGATGACGCAAAGAAGGGTAAAAGTACTCCTG
TTTACATTGGAAGGAATGACCTGCGAGTCCCGGCTAGGAGTATTTTACCCCCGTTAGATA
AGTATCCACAACCTCATGTGGAACGTATCATGGGATCTAAACAGGTGGTAGGCGCTACTG
AGATTGCTCGGGGCTGTCATCATAAATGTCTGTACTGTTCCGTCTTTGCCGCGTATAACG
GAAAGGTTGTATTGATTCCCGAAGACATTGTTTTGGAGGATGCAAAAAATTTGGTTGAGC
AGGGTATGACCCATTTGTCGTTTATTGATGCGGACTTCTTCAATGCAAAATGGCATGGAA
TTAAAATTATACGGAAGCTGCATGAAAAATATCCGTTCTTAACATATGACTTCACTACGC
GGGTGGATCACATTCTTGAGAATAGAGATACATTTCGAGAAATGGTTAATCTCGGCGTTA
AATTTGTAACTTCTGCTCTGGAATTTCCTTCAGAGGAATTACTCGATGAGGTGGAAAAGC
AAATTACTGTTAATGATATTCGCGAGGCAATCAAGTTCCTGAGAGAAATCGGAATGAAAT
TGAACCCAACATTCATTATGTTCAATCCATGGACAAGACTGGAGGATCTAATTGAGTTCA
GAGCATTTATAGAAGATAACAATTTACAAGACTTGATTGACCCCGTACAGTATCAAACAC
GACTACATCTTTACAAAGGATCTCCATTATTGATAAAAAAATCTGTAAAAAATTTAGAAT
TAACAGAGTTAGAATTCCACTATGAATGGAAACATCCAGATCCTAGAGTTGACGAGTTGT
ATAGTCAGCTTCATAAACCAGTGGAAAATGGAGTGATGAAGAGGTGTTGTTTGAAGTGCT
AACATTGGAAGTGGATAATAA
Protein sequence predicted based on nucleotide sequence
MKILLVSVFEGGFQPQTIATAAGALSEIGVEIDALDVYIEDLRFNKVSDANLVAISVPLF DSIQPAIALSKEIRKINPRTHVTFFGQHATIHAQKLASTYGDSCIRGDWEYPLINLARYL MGNNSELVGVLLADDAKKGKSTPVYIGRNDLRVPARSILPPLDKYPQPHVERIMGSKQVV GATEIARGCHHKCLYCSVFAAYNGKVVLIPEDIVLEDAKNLVEQGMTHLSFIDADFFNAK WHGIKIIRKLHEKYPFLTYDFTTRVDHILENRDTFREMVNLGVKFVTSALEFPSEELLDE VEKQITVNDIREAIKFLREIGMKLNPTFIMFNPWTRLEDLIEFRAFIEDNNLQDLIDPVQ YQTRLHLYKGSPLLIKKSVKNLELTELEFHYEWKHPDPRVDELYSQLHKPVENGVMKRCC LKC*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in MCSG-APC100356
Recommended expression in:
Not Applicable
Vector Name:
pMCSG48
Synonyms:
''
Sequencing Primer:
Forward:
pMCSG48F
Reverse:
T7 terminator
Description:
Bacterial expression vector with a T7 promoter and N-terminal 8xHis NusA tag, TEV cleavable; ampicillin resistance in bacteria; Ligation Independent cloning
Comments:
None
Size (bp):
6741
Parent Vector:
None
Properties:
bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name:
Midwest Center for Structural Genomics
PSI:Biology
Midwest Center for Structural Genomics
PSI:Biology
Midwest Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Not Available
Vector Features:
Type
Name
Description
Start
End
bacterial origin
pBR322 origin
pBR322 origin of replication
4735
4735
gene fragment
NusA
NusA cds
252
1710
ligation independent cloning
LIC
LIC site
205
234
primer
forward primer
Forward sequencing primer GAAGGGTTGACCGACGAAAAAGC
0
0
promoter
T7
T7 promoter
2345
3304
protease cleavage site
TEV
TEV protease cleavage site
223
243
repressor protein gene
LacI
LacI cds
2345
3304
selectable marker
AmpR
Ampicillin resistance gene
5493
6353
tag
His
N-terminal 8xHis tag
1717
1740
trxn termination sequence
T7 term
T7 terminator
26
72
