DNASU Plasmid | CHMP3 (H. sapiens )<br>In pGEX2T(TEV) (GST-tagged bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: RNF103-CHMP3

Gene Name: RNF103-CHMP3 readthrough

Original Clone ID:

WISP08-214

Keyword:

CHMP3 1-150 protein (aa 1-150)

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pGEX2T(TEV)

Format:

CLOSED

Source:

University of Utah

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

PMID: 19525971
Title: Structural basis for ESCRT-III protein autoinhibition.

Authors:

University of Utah
Wesley I. Sundquist
John McCullough

HsCD00520949

Price:

AVAILABLE

No restriction
Special MTA: SPTA

Insert Sequence
Protein Sequence

Insert sequence: 453nts

Open reading frame: 1 to 453

Reference Sequence Alignment

ATGGGCCTGTTTGGCAAGACCCAGGAGAAGCCGCCCAAAGAACTGGTCAATGAGTGGTCA
TTGAAGATAAGAAAGGAAATGAGAGTTGTTGACAGGCAAATAAGGGATATCCAAAGAGAA
GAAGAAAAAGTGAAACGATCTGTGAAAGATGCTGCCAAGAAGGGCCAGAAGGATGTCTGC
ATAGTTCTGGCCAAGGAGATGATCAGGTCAAGGAAGGCTGTGAGCAAGCTGTATGCATCC
AAAGCACACATGAACTCAGTGCTCATGGGGATGAAGAACCAGCTCGCGGTCTTGCGAGTG
GCTGGTTCCCTGCAGAAGAGCACAGAAGTGATGAAGGCCATGCAAAGTCTTGTGAAGATT
CCAGAGATTCAGGCCACCATGAGGGAGTTGTCCAAAGAAATGATGAAGGCTGGGATCATA
GAGGAGATGTTAGAGGACACTTTTGAAAGCTAG

Protein sequence predicted based on nucleotide sequence

MGLFGKTQEKPPKELVNEWSLKIRKEMRVVDRQIRDIQREEEKVKRSVKDAAKKGQKDVC
IVLAKEMIRSRKAVSKLYASKAHMNSVLMGMKNQLAVLRVAGSLQKSTEVMKAMQSLVKI
PEIQATMRELSKEMMKAGIIEEMLEDTFES*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

End read sequencing of cDNA insert

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinExpressed: Protein_Confirmed

ProteinPurified: Protein_Purified

SolubleProtein: Protein_Soluble

Recommended expression in:

Not Applicable

Vector Name:

pGEX2T(TEV)

Synonyms:

Sequencing Primer:

Forward: pGEX5

Reverse: pGEX3

Description:

Bacterial Expression vector with an N-terminal GST tag and TEV site; ampicillin resistance in bacteria; restriction enzyme cloning.

Comments:

Sundquist Lab internal database number = WISP 01-69; Nde1, BamH1 restriction enzyme cloning can be used easily with this vector

Size (bp):

4993

Parent Vector:

None

Properties:

bacterial expression, with tag/fusion/marker

Author Name:

University of Utah
Wesley I. Sundquist

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

Not Available

Vector Features:

Type

Name

Description

Start

End

primer

forward primer

pGEX forward sequencing primer GGTTCCGCGTGGATCTCCGG

920

939

primer

reverse primer

pGEX reverse sequencing primer CCGGGAGCTGCATGTGTCAGAGG

1043

1065

promoter

tac promoter

180

209

protease cleavage site

TEV

N-terminal TEV protease cleavage site

reporter gene

LacZ

LacZ alpha

4655

repressor binding site

lac operator

lac operator binding site

215

238

selectable marker

AmpR

Ampicillin resistance

1599

2259

tag

GST

N-terminal GST tag

258

929

Gene

Gene Symbol :

RNF103-CHMP3

Symbol Nomenclature :

RNF103-CHMP3

SYNONYM :

RNF103-VPS24

Designation :

RNF103-CHMP3 protein|RNF103-CHMP3 read-through|RNF103-VPS24 readthrough

Full Nomenclature :

RNF103-CHMP3 readthrough

GENEID :

100526767

GenBank Accession :

NP_057163

HGNC :

38847

Vega :

OTTHUMG00000160822

Target GenBank:

NP_057163

Reference Sequence Alignment

  

HsCD00520949        ------------------------------------------------------------
NM_001198954.1      ATGGAGGGTGAGCTCTATTCTGCTCTCAAGGAAGAAGAAGCATCCGAATCGGTTTCTAGT
NM_001005753.2      ------------------------------------------------------------
NM_016079.3         ------------------------------------------------------------
                                                                                

HsCD00520949        ------------------------------------------------------------
NM_001198954.1      ACCAATTTCAGTGGTGAAATGCACTTCTATGAGCTTGTGGAAGACACAAAAGATGGCATC
NM_001005753.2      ------------------------------------------------------------
NM_016079.3         ------------------------------------------------------------
                                                                                

HsCD00520949        ------------------------------------------------------------
NM_001198954.1      TGGCTGGTTCAGGTCAATGAGTGGTCATTGAAGATAAGAAAGGAAATGAGAGTTGTTGAC
NM_001005753.2      ------------------------------------------------------------
NM_016079.3         ------------------------------------------------------------
                                                                                

HsCD00520949        ------------------------------------------------------------
NM_001198954.1      AGGCAAATAAGGGATATCCAAAGAGAAGAAGAAAAAGTGAAACGATCTGTGAAAGATGCT
NM_001005753.2      ------------------------------------------------------------
NM_016079.3         ------------------------------------------------------------
                                                                                

HsCD00520949        ---------------------------------------------ATGGGCCTGTTTGGC
NM_001198954.1      GCCAAGAAGGGCCAGAAGGATGTCTGCATAGTTCTGGCCAAGGAGATGATCAGGTCAAGG
NM_001005753.2      ---------------------------------------------ATGATCAGGTCAAGG
NM_016079.3         ---------------------------------------------ATGGGGCTGTTTGGA
                                                                 ***.  . ** :.* 

HsCD00520949        AAGACCCAGGAGAAGCCGCCCAAAGAACTGGTCAATGAGTGGTCATTGAAGATAAGAAAG
NM_001198954.1      AAGGCTGTGAGCAAGCTGTATGCATCCAAAGCACACATGAACTCAGTGCTCATGGGGATG
NM_001005753.2      AAGGCTGTGAGCAAGCTGTATGCATCCAAAGCACACATGAACTCAGTGCTCATGGGGATG
NM_016079.3         AAGACCCAGGAGAAGCCGCCCAAAGAACTGGTCAATGAGTGGTCATTGAAGATAAGAAAG
                    ***.*  :*.. **** * . ..* ...:.* ..* .:*:. *** **.: **..*.*:*

HsCD00520949        GAAATGAGAGTTGTTGACAGGCAAATAAGGGATATCCAAAGAGAAGAAGAAAAAGTGAAA
NM_001198954.1      AAGAACCAGCTCGCGGTCTTGCGAGTGGCTGGTTCCCTGCAGAAGAGCACAGAAGTGATG
NM_001005753.2      AAGAACCAGCTCGCGGTCTTGCGAGTGGCTGGTTCCCTGCAGAAGAGCACAGAAGTGATG
NM_016079.3         GAAATGAGAGTTGTTGACAGGCAAATAAGGGATATCCAAAGAGAAGAAGAAAAAGTGAAA
                    .*.*: ... * *  *:*: **.*.*..  *.*: **:.....*......*.******:.

HsCD00520949        CGATCTGTGAAAGATGCTGCCAAGAAGGGCCAGAAGGATGTCTGCATAGTTCTGGCCAAG
NM_001198954.1      AAGGCCATGCAAAGTCTTGTGAAGATTCCAGAGATTCAGGCCACCATGAGGGAGTTGTCC
NM_001005753.2      AAGGCCATGCAAAGTCTTGTGAAGATTCCAGAGATTCAGGCCACCATGAGGGAGTTGTCC
NM_016079.3         CGATCTGTGAAAGATGCTGCCAAGAAGGGCCAGAAGGATGTCTGCATAGTTCTGGCCAAG
                    ... * .**.**..*  **  ****:   . ***:  * * *: ***..   :*   :. 

HsCD00520949        GAGATGATCAGGTCAAGGAAGGCTGTGAGCAAGCTGTATGCATCCAAAGCACACATGAAC
NM_001198954.1      AAAGAAATGATGAAGGCTGGGATCATAGAGGAGATGTTAGAGGACACTTTTGAAAGCATG
NM_001005753.2      AAAGAAATGATGAAGGCTGGGATCATAGAGGAGATGTTAGAGGACACTTTTGAAAGCATG
NM_016079.3         GAGATGATCAGGTCAAGGAAGGCTGTGAGCAAGCTGTATGCATCCAAAGCACACATGAAC
                    .*..:.** * *:...  ..*.  .*... .**.***::*.. .**.:  : *.*  *: 

HsCD00520949        TCAGTGCTCATGGGGATGAAGAACCAGCTCGCGGTCTTGCGAGTGGCTGGTTCCCTGCAG
NM_001198954.1      GACGATCAGGAAGAAATGGAGGAAGAAGCAGAAATGGAAATTGACAGAATTCTCTTTGAA
NM_001005753.2      GACGATCAGGAAGAAATGGAGGAAGAAGCAGAAATGGAAATTGACAGAATTCTCTTTGAA
NM_016079.3         TCAGTGCTCATGGGGATGAAGAACCAGCTCGCGGTCTTGCGAGTGGCTGGTTCCCTGCAG
                     ..*: *: .:.*..***.**.*. *.  .*...*  :.. :*: . :. *  * *  *.

HsCD00520949        AAGAGCACAGAAGTGATGAAGGCCATGCAAAGTCTTGTGAAGATTCCAGAGATTCAGGCC
NM_001198954.1      ATTACAGCAGGGGCCTTGGGCAAAGCACCCAGTAAAGTGACTGATGCCCTTCCAGAGCCA
NM_001005753.2      ATTACAGCAGGGGCCTTGGGCAAAGCACCCAGTAAAGTGACTGATGCCCTTCCAGAGCCA
NM_016079.3         AAGAGCACAGAAGTGATGAAGGCCATGCAAAGTCTTGTGAAGATTCCAGAGATTCAGGCC
                    *: * ..***..*  :**.. .... .*..***.::****. .:* *. : . : ** *.

HsCD00520949        ACCATGAGGGAGTTGTCCAAAGAAATGATGAAGGCTGGGATCATAGAGGAGATGTTAGAG
NM_001198954.1      GAACCTCCAGGAGCGATGGCTGCCTCAGAGGATGAGGAGGAGGAGGAAGAGGCTCTGGAG
NM_001005753.2      GAACCTCCAGGAGCGATGGCTGCCTCAGAGGATGAGGAGGAGGAGGAAGAGGCTCTGGAG
NM_016079.3         ACCATGAGGGAGTTGTCCAAAGAAATGATGAAGGCTGGGATCATAGAGGAGATGTTAGAG
                    ....  . .*..  *:  ..:*..: ..:*.* *. *.*.: .:.**.***.   *.***

HsCD00520949        GACACTTTTGAAAGCTAG------------------------------------------
NM_001198954.1      GCCATGCAGTCCCGGCTGGCCACACTCCGCAGCTAG------------------------
NM_001005753.2      GCCATGCAGTCCCGGCTGGCCACACTCCGCAGCTAG------------------------
NM_016079.3         GACACTTTTGAAAGCATGGACGATCAGGAAGAAATGGAGGAAGAAGCAGAAATGGAAATT
                    *.**   :  ...*  :*                                          

HsCD00520949        ------------------------------------------------------------
NM_001198954.1      ------------------------------------------------------------
NM_001005753.2      ------------------------------------------------------------
NM_016079.3         GACAGAATTCTCTTTGAAATTACAGCAGGGGCCTTGGGCAAAGCACCCAGTAAAGTGACT
                                                                                

HsCD00520949        ------------------------------------------------------------
NM_001198954.1      ------------------------------------------------------------
NM_001005753.2      ------------------------------------------------------------
NM_016079.3         GATGCCCTTCCAGAGCCAGAACCTCCAGGAGCGATGGCTGCCTCAGAGGATGAGGAGGAG
                                                                                

HsCD00520949        ------------------------------------------------------
NM_001198954.1      ------------------------------------------------------
NM_001005753.2      ------------------------------------------------------
NM_016079.3         GAGGAAGAGGCTCTGGAGGCCATGCAGTCCCGGCTGGCCACACTCCGCAGCTAG

Pathways

NCI :

Internalization of ErbB1

Reactome :

Budding and maturation of HIV virion

Reactome :

Disease

Reactome :

Endosomal Sorting Complex Required For Transport (ESCRT)

Reactome :

HIV Infection

Reactome :

HIV Life Cycle

Reactome :

Late Phase of HIV Life Cycle

Reactome :

Membrane Trafficking

Clone

Cloning Information :

582311

Original Clone ID :

WISP08-214

Organism

Species Specific ID:

51652

Genome

Chromosome :

Map Location :

Ensembl :

ENSG00000115561

Ensembl :

ENSG00000249884

Reagents

Labome :

CHMP3-antibody

CHMP3 (H. sapiens )In pGEX2T(TEV) (GST-tagged bacterial expression vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Reference Sequence Alignment

Pathways

Clone

Organism

Genome

Reagents

CHMP3 (H. sapiens )
In pGEX2T(TEV) (GST-tagged bacterial expression vector)