DNASU Plasmid | CHMP3 (H. sapiens )<br>In pGEX2T(TEV) (GST-tagged bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: RNF103-CHMP3

Gene Name: RNF103-CHMP3 readthrough

Original Clone ID:

WISP08-215

Keyword:

CHMP3 I168D L169D

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pGEX2T(TEV)

Format:

CLOSED

Source:

University of Utah

Description:

None

Comments:

None

Mutation / Discrepancy:

Yes / No

Publications:

PMID: 19525971
Title: Structural basis for ESCRT-III protein autoinhibition.

Authors:

University of Utah
Wesley I. Sundquist
John McCullough

HsCD00520954

Price:

AVAILABLE

No restriction
Special MTA: SPTA

Insert Sequence
Protein Sequence

Insert sequence: 669nts

Open reading frame: 1 to 669

Reference Sequence Alignment

ATGGGCCTGTTTGGCAAGACCCAGGAGAAGCCGCCCAAAGAACTGGTCAATGAGTGGTCA
TTGAAGATAAGAAAGGAAATGAGAGTTGTTGACAGGCAAATAAGGGATATCCAAAGAGAA
GAAGAAAAAGTGAAACGATCTGTGAAAGATGCTGCCAAGAAGGGCCAGAAGGATGTCTGC
ATAGTTCTGGCCAAGGAGATGATCAGGTCAAGGAAGGCTGTGAGCAAGCTGTATGCATCC
AAAGCACACATGAACTCAGTGCTCATGGGGATGAAGAACCAGCTCGCGGTCTTGCGAGTG
GCTGGTTCCCTGCAGAAGAGCACAGAAGTGATGAAGGCCATGCAAAGTCTTGTGAAGATT
CCAGAGATTCAGGCCACCATGAGGGAGTTGTCCAAAGAAATGATGAAGGCTGGGATCATA
GAGGAGATGTTAGAGGACACTTTTGAAAGCATGGACGATCAGGAAGAAATGGAGGAAGAA
GCAGAAATGGAAATTGACAGAATTCTCTTTGAAATTACAGCAGGGGCCTTGGGCAAAGCA
CCCAGTAAAGTGACTGATGCCCTTCCAGAGCCAGAACCTCCAGGAGCGATGGCTGCCTCA
GAGGATGAGGAGGAGGAGGAAGAGGCTCTGGAGGCCATGCAGTCCCGGCTGGCCACACTC
CGCAGCTAG

Protein sequence predicted based on nucleotide sequence

MGLFGKTQEKPPKELVNEWSLKIRKEMRVVDRQIRDIQREEEKVKRSVKDAAKKGQKDVC
IVLAKEMIRSRKAVSKLYASKAHMNSVLMGMKNQLAVLRVAGSLQKSTEVMKAMQSLVKI
PEIQATMRELSKEMMKAGIIEEMLEDTFESMDDQEEMEEEAEMEIDRILFEITAGALGKA
PSKVTDALPEPEPPGAMAASEDEEEEEEALEAMQSRLATLRS*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

End read sequencing of cDNA insert

Mutations:

I168D L169D

a502g; t503a; c505g; t506a

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinExpressed: Protein_Confirmed

ProteinPurified: Protein_Purified

SolubleProtein: Protein_Soluble

Recommended expression in:

Not Applicable

Vector Name:

pGEX2T(TEV)

Synonyms:

Sequencing Primer:

Forward: pGEX5

Reverse: pGEX3

Description:

Bacterial Expression vector with an N-terminal GST tag and TEV site; ampicillin resistance in bacteria; restriction enzyme cloning.

Comments:

Sundquist Lab internal database number = WISP 01-69; Nde1, BamH1 restriction enzyme cloning can be used easily with this vector

Size (bp):

4993

Parent Vector:

None

Properties:

bacterial expression, with tag/fusion/marker

Author Name:

University of Utah
Wesley I. Sundquist

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

Not Available

Vector Features:

Type

Name

Description

Start

End

primer

forward primer

pGEX forward sequencing primer GGTTCCGCGTGGATCTCCGG

920

939

primer

reverse primer

pGEX reverse sequencing primer CCGGGAGCTGCATGTGTCAGAGG

1043

1065

promoter

tac promoter

180

209

protease cleavage site

TEV

N-terminal TEV protease cleavage site

reporter gene

LacZ

LacZ alpha

4655

repressor binding site

lac operator

lac operator binding site

215

238

selectable marker

AmpR

Ampicillin resistance

1599

2259

tag

GST

N-terminal GST tag

258

929

Gene

Gene Symbol :

RNF103-CHMP3

Symbol Nomenclature :

RNF103-CHMP3

SYNONYM :

RNF103-VPS24

Designation :

RNF103-CHMP3 protein|RNF103-CHMP3 read-through|RNF103-VPS24 readthrough

Full Nomenclature :

RNF103-CHMP3 readthrough

GENEID :

100526767

GenBank Accession :

NP_057163

HGNC :

38847

Vega :

OTTHUMG00000160822

Target GenBank:

NP_057163

Reference Sequence Alignment

  

HsCD00520954        ------------------------------------------------------------
NM_001198954.1      ATGGAGGGTGAGCTCTATTCTGCTCTCAAGGAAGAAGAAGCATCCGAATCGGTTTCTAGT
NM_016079.3         ------------------------------------------------------------
                                                                                

HsCD00520954        ---------------------------ATGGGCCTGTTTGGCAAGACCCAGGAGAAGCCG
NM_001198954.1      ACCAATTTCAGTGGTGAAATGCACTTCTATGAGCTTGTGGAAGACACAAAAGATGGCATC
NM_016079.3         ---------------------------ATGGGGCTGTTTGGAAAGACCCAGGAGAAGCCG
                                               :: *. **  * *...* **..*.** .. .  

HsCD00520954        CCCAAAGAACTGGTCAATGAGTGGTCATTGAAGATAAGAAAGGAAATGAGAGTTGTTGAC
NM_001198954.1      TGGCTGGTTCAGGTCAATGAGTGGTCATTGAAGATAAGAAAGGAAATGAGAGTTGTTGAC
NM_016079.3         CCCAAAGAACTGGTCAATGAGTGGTCATTGAAGATAAGAAAGGAAATGAGAGTTGTTGAC
                       .:.*::*:*************************************************

HsCD00520954        AGGCAAATAAGGGATATCCAAAGAGAAGAAGAAAAAGTGAAACGATCTGTGAAAGATGCT
NM_001198954.1      AGGCAAATAAGGGATATCCAAAGAGAAGAAGAAAAAGTGAAACGATCTGTGAAAGATGCT
NM_016079.3         AGGCAAATAAGGGATATCCAAAGAGAAGAAGAAAAAGTGAAACGATCTGTGAAAGATGCT
                    ************************************************************

HsCD00520954        GCCAAGAAGGGCCAGAAGGATGTCTGCATAGTTCTGGCCAAGGAGATGATCAGGTCAAGG
NM_001198954.1      GCCAAGAAGGGCCAGAAGGATGTCTGCATAGTTCTGGCCAAGGAGATGATCAGGTCAAGG
NM_016079.3         GCCAAGAAGGGCCAGAAGGATGTCTGCATAGTTCTGGCCAAGGAGATGATCAGGTCAAGG
                    ************************************************************

HsCD00520954        AAGGCTGTGAGCAAGCTGTATGCATCCAAAGCACACATGAACTCAGTGCTCATGGGGATG
NM_001198954.1      AAGGCTGTGAGCAAGCTGTATGCATCCAAAGCACACATGAACTCAGTGCTCATGGGGATG
NM_016079.3         AAGGCTGTGAGCAAGCTGTATGCATCCAAAGCACACATGAACTCAGTGCTCATGGGGATG
                    ************************************************************

HsCD00520954        AAGAACCAGCTCGCGGTCTTGCGAGTGGCTGGTTCCCTGCAGAAGAGCACAGAAGTGATG
NM_001198954.1      AAGAACCAGCTCGCGGTCTTGCGAGTGGCTGGTTCCCTGCAGAAGAGCACAGAAGTGATG
NM_016079.3         AAGAACCAGCTCGCGGTCTTGCGAGTGGCTGGTTCCCTGCAGAAGAGCACAGAAGTGATG
                    ************************************************************

HsCD00520954        AAGGCCATGCAAAGTCTTGTGAAGATTCCAGAGATTCAGGCCACCATGAGGGAGTTGTCC
NM_001198954.1      AAGGCCATGCAAAGTCTTGTGAAGATTCCAGAGATTCAGGCCACCATGAGGGAGTTGTCC
NM_016079.3         AAGGCCATGCAAAGTCTTGTGAAGATTCCAGAGATTCAGGCCACCATGAGGGAGTTGTCC
                    ************************************************************

HsCD00520954        AAAGAAATGATGAAGGCTGGGATCATAGAGGAGATGTTAGAGGACACTTTTGAAAGCATG
NM_001198954.1      AAAGAAATGATGAAGGCTGGGATCATAGAGGAGATGTTAGAGGACACTTTTGAAAGCATG
NM_016079.3         AAAGAAATGATGAAGGCTGGGATCATAGAGGAGATGTTAGAGGACACTTTTGAAAGCATG
                    ************************************************************

HsCD00520954        GACGATCAGGAAGAAATGGAGGAAGAAGCAGAAATGGAAATTGACAGAATTCTCTTTGAA
NM_001198954.1      GACGATCAGGAAGAAATGGAGGAAGAAGCAGAAATGGAAATTGACAGAATTCTCTTTGAA
NM_016079.3         GACGATCAGGAAGAAATGGAGGAAGAAGCAGAAATGGAAATTGACAGAATTCTCTTTGAA
                    ************************************************************

HsCD00520954        ATTACAGCAGGGGCCTTGGGCAAAGCACCCAGTAAAGTGACTGATGCCCTTCCAGAGCCA
NM_001198954.1      ATTACAGCAGGGGCCTTGGGCAAAGCACCCAGTAAAGTGACTGATGCCCTTCCAGAGCCA
NM_016079.3         ATTACAGCAGGGGCCTTGGGCAAAGCACCCAGTAAAGTGACTGATGCCCTTCCAGAGCCA
                    ************************************************************

HsCD00520954        GAACCTCCAGGAGCGATGGCTGCCTCAGAGGATGAGGAGGAGGAGGAAGAGGCTCTGGAG
NM_001198954.1      GAACCTCCAGGAGCGATGGCTGCCTCAGAGGATGAGGAGGAGGAGGAAGAGGCTCTGGAG
NM_016079.3         GAACCTCCAGGAGCGATGGCTGCCTCAGAGGATGAGGAGGAGGAGGAAGAGGCTCTGGAG
                    ************************************************************

HsCD00520954        GCCATGCAGTCCCGGCTGGCCACACTCCGCAGCTAG
NM_001198954.1      GCCATGCAGTCCCGGCTGGCCACACTCCGCAGCTAG
NM_016079.3         GCCATGCAGTCCCGGCTGGCCACACTCCGCAGCTAG
                    ************************************

Pathways

NCI :

Internalization of ErbB1

Reactome :

Budding and maturation of HIV virion

Reactome :

Disease

Reactome :

Endosomal Sorting Complex Required For Transport (ESCRT)

Reactome :

HIV Infection

Reactome :

HIV Life Cycle

Reactome :

Late Phase of HIV Life Cycle

Reactome :

Membrane Trafficking

Clone

Cloning Information :

582316

Original Clone ID :

WISP08-215

Organism

Species Specific ID:

51652

Genome

Chromosome :

Map Location :

Ensembl :

ENSG00000115561

Ensembl :

ENSG00000249884

Reagents

Labome :

CHMP3-antibody

CHMP3 (H. sapiens )In pGEX2T(TEV) (GST-tagged bacterial expression vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Reference Sequence Alignment

Pathways

Clone

Organism

Genome

Reagents

CHMP3 (H. sapiens )
In pGEX2T(TEV) (GST-tagged bacterial expression vector)