DNASU Plasmid | CHMP3 (H. sapiens )<br>In pCAG.MCS2.C-Myc (Myc-tagged mammalian expression vector)

Explanation of Terms

Gene:

Gene Symbol: RNF103-CHMP3

Gene Name: RNF103-CHMP3 readthrough

Original Clone ID:

WISP08-208

Keyword:

CHMP3 1-150 protein (aa 1-150)

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pCAG.MCS2.C-Myc

Format:

FUSION

Source:

University of Utah

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

PMID: 19525971
Title: Structural basis for ESCRT-III protein autoinhibition.

Authors:

University of Utah
Wesley I. Sundquist
John McCullough

HsCD00520972

Price:

AVAILABLE

No restriction
Special MTA: SPTA

Insert Sequence
Protein Sequence

Insert sequence: 450nts

Open reading frame: 1 to 450

Reference Sequence Alignment

ATGGGCCTGTTTGGCAAGACCCAGGAGAAGCCGCCCAAAGAACTGGTCAATGAGTGGTCA
TTGAAGATAAGAAAGGAAATGAGAGTTGTTGACAGGCAAATAAGGGATATCCAAAGAGAA
GAAGAAAAAGTGAAACGATCTGTGAAAGATGCTGCCAAGAAGGGCCAGAAGGATGTCTGC
ATAGTTCTGGCCAAGGAGATGATCAGGTCAAGGAAGGCTGTGAGCAAGCTGTATGCATCC
AAAGCACACATGAACTCAGTGCTCATGGGGATGAAGAACCAGCTCGCGGTCTTGCGAGTG
GCTGGTTCCCTGCAGAAGAGCACAGAAGTGATGAAGGCCATGCAAAGTCTTGTGAAGATT
CCAGAGATTCAGGCCACCATGAGGGAGTTGTCCAAAGAAATGATGAAGGCTGGGATCATA
GAGGAGATGTTAGAGGACACTTTTGAAAGC

Protein sequence predicted based on nucleotide sequence

MGLFGKTQEKPPKELVNEWSLKIRKEMRVVDRQIRDIQREEEKVKRSVKDAAKKGQKDVC
IVLAKEMIRSRKAVSKLYASKAHMNSVLMGMKNQLAVLRVAGSLQKSTEVMKAMQSLVKI
PEIQATMRELSKEMMKAGIIEEMLEDTFES

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

End read sequencing of cDNA insert

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: chloramphenicol, ampicillin

Growth Condition: LB with 35ug/ml chloramphenicol and 100ug/ml ampicillin

Comments: None

Protein Expression Results:

ProteinExpressed: Not_Applicable

ProteinPurified: Not_Applicable

SolubleProtein: Not_Applicable

Recommended expression in:

Not Applicable

Vector Name:

pCAG.MCS2.C-Myc

Synonyms:

Sequencing Primer:

Forward: Unknown

Reverse: Unknown

Description:

Mammalian expression vector with a C-terminal myc tag; ampicillin resistance in bacteria; restriction enzyme cloning.

Comments:

Sundquist Lab internal database number = WISP 08-104; Restriction enzyme cloning can be used with this vector - e.g. Xho1, Kpn1 works well

Size (bp):

4813

Parent Vector:

None

Properties:

mammalian expression, multiple cloning site, with tag/fusion/marker

Author Name:

University of Utah
Wesley I. Sundquist

Publications:

PMID: 19525971

Title: Structural basis for ESCRT-III protein autoinhibition.

Vector Map:

Vector Sequence:

Sequence:

Not Available

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ColE

ColE1 origin

3045

3727

primer

forward primer

CAGF forward sequencing primer

1605

1624

primer

reverse primer

CAGR reverse sequencing primer TGTCCCCATAATTTTTGGCAGAGG

1845

1868

selectable marker

AmpR

Ampicillin resistance

3824

4484

tag

c-myc

C-terminal may tag

Gene

Gene Symbol :

RNF103-CHMP3

Symbol Nomenclature :

RNF103-CHMP3

SYNONYM :

RNF103-VPS24

Designation :

RNF103-CHMP3 protein|RNF103-CHMP3 read-through|RNF103-VPS24 readthrough

Full Nomenclature :

RNF103-CHMP3 readthrough

GENEID :

100526767

GenBank Accession :

NP_057163

HGNC :

38847

Vega :

OTTHUMG00000160822

Target GenBank:

NP_057163

Reference Sequence Alignment

  

HsCD00520972        ------------------------------------------------------------
NM_001198954.1      ATGGAGGGTGAGCTCTATTCTGCTCTCAAGGAAGAAGAAGCATCCGAATCGGTTTCTAGT
NM_001005753.2      ------------------------------------------------------------
NM_016079.3         ------------------------------------------------------------
                                                                                

HsCD00520972        ------------------------------------------------------------
NM_001198954.1      ACCAATTTCAGTGGTGAAATGCACTTCTATGAGCTTGTGGAAGACACAAAAGATGGCATC
NM_001005753.2      ------------------------------------------------------------
NM_016079.3         ------------------------------------------------------------
                                                                                

HsCD00520972        ------------------------------------------------------------
NM_001198954.1      TGGCTGGTTCAGGTCAATGAGTGGTCATTGAAGATAAGAAAGGAAATGAGAGTTGTTGAC
NM_001005753.2      ------------------------------------------------------------
NM_016079.3         ------------------------------------------------------------
                                                                                

HsCD00520972        ------------------------------------------------------------
NM_001198954.1      AGGCAAATAAGGGATATCCAAAGAGAAGAAGAAAAAGTGAAACGATCTGTGAAAGATGCT
NM_001005753.2      ------------------------------------------------------------
NM_016079.3         ------------------------------------------------------------
                                                                                

HsCD00520972        ---------------------------------------------ATGGGCCTGTTTGGC
NM_001198954.1      GCCAAGAAGGGCCAGAAGGATGTCTGCATAGTTCTGGCCAAGGAGATGATCAGGTCAAGG
NM_001005753.2      ---------------------------------------------ATGATCAGGTCAAGG
NM_016079.3         ---------------------------------------------ATGGGGCTGTTTGGA
                                                                 ***.  . ** :.* 

HsCD00520972        AAGACCCAGGAGAAGCCGCCCAAAGAACTGGTCAATGAGTGGTCATTGAAGATAAGAAAG
NM_001198954.1      AAGGCTGTGAGCAAGCTGTATGCATCCAAAGCACACATGAACTCAGTGCTCATGGGGATG
NM_001005753.2      AAGGCTGTGAGCAAGCTGTATGCATCCAAAGCACACATGAACTCAGTGCTCATGGGGATG
NM_016079.3         AAGACCCAGGAGAAGCCGCCCAAAGAACTGGTCAATGAGTGGTCATTGAAGATAAGAAAG
                    ***.*  :*.. **** * . ..* ...:.* ..* .:*:. *** **.: **..*.*:*

HsCD00520972        GAAATGAGAGTTGTTGACAGGCAAATAAGGGATATCCAAAGAGAAGAAGAAAAAGTGAAA
NM_001198954.1      AAGAACCAGCTCGCGGTCTTGCGAGTGGCTGGTTCCCTGCAGAAGAGCACAGAAGTGATG
NM_001005753.2      AAGAACCAGCTCGCGGTCTTGCGAGTGGCTGGTTCCCTGCAGAAGAGCACAGAAGTGATG
NM_016079.3         GAAATGAGAGTTGTTGACAGGCAAATAAGGGATATCCAAAGAGAAGAAGAAAAAGTGAAA
                    .*.*: ... * *  *:*: **.*.*..  *.*: **:.....*......*.******:.

HsCD00520972        CGATCTGTGAAAGATGCTGCCAAGAAGGGCCAGAAGGATGTCTGCATAGTTCTGGCCAAG
NM_001198954.1      AAGGCCATGCAAAGTCTTGTGAAGATTCCAGAGATTCAGGCCACCATGAGGGAGTTGTCC
NM_001005753.2      AAGGCCATGCAAAGTCTTGTGAAGATTCCAGAGATTCAGGCCACCATGAGGGAGTTGTCC
NM_016079.3         CGATCTGTGAAAGATGCTGCCAAGAAGGGCCAGAAGGATGTCTGCATAGTTCTGGCCAAG
                    ... * .**.**..*  **  ****:   . ***:  * * *: ***..   :*   :. 

HsCD00520972        GAGATGATCAGGTCAAGGAAGGCTGTGAGCAAGCTGTATGCATCCAAAGCACACATGAAC
NM_001198954.1      AAAGAAATGATGAAGGCTGGGATCATAGAGGAGATGTTAGAGGACACTTTTGAAAGCATG
NM_001005753.2      AAAGAAATGATGAAGGCTGGGATCATAGAGGAGATGTTAGAGGACACTTTTGAAAGCATG
NM_016079.3         GAGATGATCAGGTCAAGGAAGGCTGTGAGCAAGCTGTATGCATCCAAAGCACACATGAAC
                    .*..:.** * *:...  ..*.  .*... .**.***::*.. .**.:  : *.*  *: 

HsCD00520972        TCAGTGCTCATGGGGATGAAGAACCAGCTCGCGGTCTTGCGAGTGGCTGGTTCCCTGCAG
NM_001198954.1      GACGATCAGGAAGAAATGGAGGAAGAAGCAGAAATGGAAATTGACAGAATTCTCTTTGAA
NM_001005753.2      GACGATCAGGAAGAAATGGAGGAAGAAGCAGAAATGGAAATTGACAGAATTCTCTTTGAA
NM_016079.3         TCAGTGCTCATGGGGATGAAGAACCAGCTCGCGGTCTTGCGAGTGGCTGGTTCCCTGCAG
                     ..*: *: .:.*..***.**.*. *.  .*...*  :.. :*: . :. *  * *  *.

HsCD00520972        AAGAGCACAGAAGTGATGAAGGCCATGCAAAGTCTTGTGAAGATTCCAGAGATTCAGGCC
NM_001198954.1      ATTACAGCAGGGGCCTTGGGCAAAGCACCCAGTAAAGTGACTGATGCCCTTCCAGAGCCA
NM_001005753.2      ATTACAGCAGGGGCCTTGGGCAAAGCACCCAGTAAAGTGACTGATGCCCTTCCAGAGCCA
NM_016079.3         AAGAGCACAGAAGTGATGAAGGCCATGCAAAGTCTTGTGAAGATTCCAGAGATTCAGGCC
                    *: * ..***..*  :**.. .... .*..***.::****. .:* *. : . : ** *.

HsCD00520972        ACCATGAGGGAGTTGTCCAAAGAAATGATGAAGGCTGGGATCATAGAGGAGATGTTAGAG
NM_001198954.1      GAACCTCCAGGAGCGATGGCTGCCTCAGAGGATGAGGAGGAGGAGGAAGAGGCTCTGGAG
NM_001005753.2      GAACCTCCAGGAGCGATGGCTGCCTCAGAGGATGAGGAGGAGGAGGAAGAGGCTCTGGAG
NM_016079.3         ACCATGAGGGAGTTGTCCAAAGAAATGATGAAGGCTGGGATCATAGAGGAGATGTTAGAG
                    ....  . .*..  *:  ..:*..: ..:*.* *. *.*.: .:.**.***.   *.***

HsCD00520972        GACACTTTTGAAAGC---------------------------------------------
NM_001198954.1      GCCATGCAGTCCCGGCTGGCCACACTCCGCAGCTAG------------------------
NM_001005753.2      GCCATGCAGTCCCGGCTGGCCACACTCCGCAGCTAG------------------------
NM_016079.3         GACACTTTTGAAAGCATGGACGATCAGGAAGAAATGGAGGAAGAAGCAGAAATGGAAATT
                    *.**   :  ...*                                              

HsCD00520972        ------------------------------------------------------------
NM_001198954.1      ------------------------------------------------------------
NM_001005753.2      ------------------------------------------------------------
NM_016079.3         GACAGAATTCTCTTTGAAATTACAGCAGGGGCCTTGGGCAAAGCACCCAGTAAAGTGACT
                                                                                

HsCD00520972        ------------------------------------------------------------
NM_001198954.1      ------------------------------------------------------------
NM_001005753.2      ------------------------------------------------------------
NM_016079.3         GATGCCCTTCCAGAGCCAGAACCTCCAGGAGCGATGGCTGCCTCAGAGGATGAGGAGGAG
                                                                                

HsCD00520972        ------------------------------------------------------
NM_001198954.1      ------------------------------------------------------
NM_001005753.2      ------------------------------------------------------
NM_016079.3         GAGGAAGAGGCTCTGGAGGCCATGCAGTCCCGGCTGGCCACACTCCGCAGCTAG

Pathways

NCI :

Internalization of ErbB1

Reactome :

Budding and maturation of HIV virion

Reactome :

Disease

Reactome :

Endosomal Sorting Complex Required For Transport (ESCRT)

Reactome :

HIV Infection

Reactome :

HIV Life Cycle

Reactome :

Late Phase of HIV Life Cycle

Reactome :

Membrane Trafficking

Clone

Cloning Information :

582334

Original Clone ID :

WISP08-208

Organism

Species Specific ID:

51652

Genome

Chromosome :

Map Location :

Ensembl :

ENSG00000115561

Ensembl :

ENSG00000249884

Reagents

Labome :

CHMP3-antibody

CHMP3 (H. sapiens )In pCAG.MCS2.C-Myc (Myc-tagged mammalian expression vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Reference Sequence Alignment

Pathways

Clone

Organism

Genome

Reagents

CHMP3 (H. sapiens )
In pCAG.MCS2.C-Myc (Myc-tagged mammalian expression vector)