DNASU Plasmid | CHMP3 (H. sapiens )<br>In pCAG.MCS2.C-Myc (Myc-tagged mammalian expression vector)

Explanation of Terms

Gene:

Gene Symbol: RNF103-CHMP3

Gene Name: RNF103-CHMP3 readthrough

Original Clone ID:

WISP09-235

Keyword:

CHMP3 full length protein (aa 1-222) V48D I168D L169D

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pCAG.MCS2.C-Myc

Format:

FUSION

Source:

University of Utah

Description:

None

Comments:

None

Mutation / Discrepancy:

Yes / No

Publications:

PMID: 19525971
Title: Structural basis for ESCRT-III protein autoinhibition.

Authors:

University of Utah
Wesley I. Sundquist
John McCullough

HsCD00520982

Price:

AVAILABLE

No restriction
Special MTA: SPTA

Insert Sequence
Protein Sequence

Insert sequence: 666nts

Open reading frame: 1 to 666

Reference Sequence Alignment

ATGGGGCTGTTTGGAAAGACCCAGGAGAAGCCGCCCAAAGAACTGGTCAATGAGTGGTCA
TTGAAGATAAGAAAGGAAATGAGAGTTGTTGACAGGCAAATAAGGGATATCCAAAGAGAA
GAAGAAAAAGTGAAACGATCTGTGAAAGATGCTGCCAAGAAGGGCCAGAAGGATGTCTGC
ATAGTTCTGGCCAAGGAGATGATCAGGTCAAGGAAGGCTGTGAGCAAGCTGTATGCATCC
AAAGCACACATGAACTCAGTGCTCATGGGGATGAAGAACCAGCTCGCGGTCTTGCGAGTG
GCTGGTTCCCTGCAGAAGAGCACAGAAGTGATGAAGGCCATGCAAAGTCTTGTGAAGATT
CCAGAGATTCAGGCCACCATGAGGGAGTTGTCCAAAGAAATGATGAAGGCTGGGATCATA
GAGGAGATGTTAGAGGACACTTTTGAAAGCATGGACGATCAGGAGGAGATGGAAGAGGAA
GCAGAAATGGAAATTGACAGAATTCTCTTTGAAATTACAGCAGGGGCCTTGGGCAAAGCA
CCCAGTAAAGTGACTGATGCCCTTCCAGAGCCAGAACCTCCAGGAGCGATGGCTGCCTCA
GAGGATGAGGAGGAGGAGGAAGAGGCTCTGGAGGCCATGCAGTCCCGGCTGGCCACACTC
CGCAGC

Protein sequence predicted based on nucleotide sequence

MGLFGKTQEKPPKELVNEWSLKIRKEMRVVDRQIRDIQREEEKVKRSVKDAAKKGQKDVC
IVLAKEMIRSRKAVSKLYASKAHMNSVLMGMKNQLAVLRVAGSLQKSTEVMKAMQSLVKI
PEIQATMRELSKEMMKAGIIEEMLEDTFESMDDQEEMEEEAEMEIDRILFEITAGALGKA
PSKVTDALPEPEPPGAMAASEDEEEEEEALEAMQSRLATLRS

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

End read sequencing of cDNA insert

Mutations:

V48D I168D L169D

at143a; g144c; 502g; t503a; c505g; t506a

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: chloramphenicol, ampicillin

Growth Condition: LB with 35ug/ml chloramphenicol and 100ug/ml ampicillin

Comments: None

Protein Expression Results:

ProteinExpressed: Not_Applicable

ProteinPurified: Not_Applicable

SolubleProtein: Not_Applicable

Recommended expression in:

Not Applicable

Vector Name:

pCAG.MCS2.C-Myc

Synonyms:

Sequencing Primer:

Forward: Unknown

Reverse: Unknown

Description:

Mammalian expression vector with a C-terminal myc tag; ampicillin resistance in bacteria; restriction enzyme cloning.

Comments:

Sundquist Lab internal database number = WISP 08-104; Restriction enzyme cloning can be used with this vector - e.g. Xho1, Kpn1 works well

Size (bp):

4813

Parent Vector:

None

Properties:

mammalian expression, multiple cloning site, with tag/fusion/marker

Author Name:

University of Utah
Wesley I. Sundquist

Publications:

PMID: 19525971

Title: Structural basis for ESCRT-III protein autoinhibition.

Vector Map:

Vector Sequence:

Sequence:

Not Available

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ColE

ColE1 origin

3045

3727

primer

forward primer

CAGF forward sequencing primer

1605

1624

primer

reverse primer

CAGR reverse sequencing primer TGTCCCCATAATTTTTGGCAGAGG

1845

1868

selectable marker

AmpR

Ampicillin resistance

3824

4484

tag

c-myc

C-terminal may tag

Gene

Gene Symbol :

RNF103-CHMP3

Symbol Nomenclature :

RNF103-CHMP3

SYNONYM :

RNF103-VPS24

Designation :

RNF103-CHMP3 protein|RNF103-CHMP3 read-through|RNF103-VPS24 readthrough

Full Nomenclature :

RNF103-CHMP3 readthrough

GENEID :

100526767

GenBank Accession :

NP_057163

HGNC :

38847

Vega :

OTTHUMG00000160822

Target GenBank:

NP_057163

Reference Sequence Alignment

  

HsCD00520982        ------------------------------------------------------------
NM_001198954.1      ATGGAGGGTGAGCTCTATTCTGCTCTCAAGGAAGAAGAAGCATCCGAATCGGTTTCTAGT
NM_016079.3         ------------------------------------------------------------
                                                                                

HsCD00520982        ---------------------------ATGGGGCTGTTTGGAAAGACCCAGGAGAAGCCG
NM_001198954.1      ACCAATTTCAGTGGTGAAATGCACTTCTATGAGCTTGTGGAAGACACAAAAGATGGCATC
NM_016079.3         ---------------------------ATGGGGCTGTTTGGAAAGACCCAGGAGAAGCCG
                                               :: *.***  * *.*.* **..*.** .. .  

HsCD00520982        CCCAAAGAACTGGTCAATGAGTGGTCATTGAAGATAAGAAAGGAAATGAGAGTTGTTGAC
NM_001198954.1      TGGCTGGTTCAGGTCAATGAGTGGTCATTGAAGATAAGAAAGGAAATGAGAGTTGTTGAC
NM_016079.3         CCCAAAGAACTGGTCAATGAGTGGTCATTGAAGATAAGAAAGGAAATGAGAGTTGTTGAC
                       .:.*::*:*************************************************

HsCD00520982        AGGCAAATAAGGGATATCCAAAGAGAAGAAGAAAAAGTGAAACGATCTGTGAAAGATGCT
NM_001198954.1      AGGCAAATAAGGGATATCCAAAGAGAAGAAGAAAAAGTGAAACGATCTGTGAAAGATGCT
NM_016079.3         AGGCAAATAAGGGATATCCAAAGAGAAGAAGAAAAAGTGAAACGATCTGTGAAAGATGCT
                    ************************************************************

HsCD00520982        GCCAAGAAGGGCCAGAAGGATGTCTGCATAGTTCTGGCCAAGGAGATGATCAGGTCAAGG
NM_001198954.1      GCCAAGAAGGGCCAGAAGGATGTCTGCATAGTTCTGGCCAAGGAGATGATCAGGTCAAGG
NM_016079.3         GCCAAGAAGGGCCAGAAGGATGTCTGCATAGTTCTGGCCAAGGAGATGATCAGGTCAAGG
                    ************************************************************

HsCD00520982        AAGGCTGTGAGCAAGCTGTATGCATCCAAAGCACACATGAACTCAGTGCTCATGGGGATG
NM_001198954.1      AAGGCTGTGAGCAAGCTGTATGCATCCAAAGCACACATGAACTCAGTGCTCATGGGGATG
NM_016079.3         AAGGCTGTGAGCAAGCTGTATGCATCCAAAGCACACATGAACTCAGTGCTCATGGGGATG
                    ************************************************************

HsCD00520982        AAGAACCAGCTCGCGGTCTTGCGAGTGGCTGGTTCCCTGCAGAAGAGCACAGAAGTGATG
NM_001198954.1      AAGAACCAGCTCGCGGTCTTGCGAGTGGCTGGTTCCCTGCAGAAGAGCACAGAAGTGATG
NM_016079.3         AAGAACCAGCTCGCGGTCTTGCGAGTGGCTGGTTCCCTGCAGAAGAGCACAGAAGTGATG
                    ************************************************************

HsCD00520982        AAGGCCATGCAAAGTCTTGTGAAGATTCCAGAGATTCAGGCCACCATGAGGGAGTTGTCC
NM_001198954.1      AAGGCCATGCAAAGTCTTGTGAAGATTCCAGAGATTCAGGCCACCATGAGGGAGTTGTCC
NM_016079.3         AAGGCCATGCAAAGTCTTGTGAAGATTCCAGAGATTCAGGCCACCATGAGGGAGTTGTCC
                    ************************************************************

HsCD00520982        AAAGAAATGATGAAGGCTGGGATCATAGAGGAGATGTTAGAGGACACTTTTGAAAGCATG
NM_001198954.1      AAAGAAATGATGAAGGCTGGGATCATAGAGGAGATGTTAGAGGACACTTTTGAAAGCATG
NM_016079.3         AAAGAAATGATGAAGGCTGGGATCATAGAGGAGATGTTAGAGGACACTTTTGAAAGCATG
                    ************************************************************

HsCD00520982        GACGATCAGGAGGAGATGGAAGAGGAAGCAGAAATGGAAATTGACAGAATTCTCTTTGAA
NM_001198954.1      GACGATCAGGAAGAAATGGAGGAAGAAGCAGAAATGGAAATTGACAGAATTCTCTTTGAA
NM_016079.3         GACGATCAGGAAGAAATGGAGGAAGAAGCAGAAATGGAAATTGACAGAATTCTCTTTGAA
                    ***********.**.*****.**.************************************

HsCD00520982        ATTACAGCAGGGGCCTTGGGCAAAGCACCCAGTAAAGTGACTGATGCCCTTCCAGAGCCA
NM_001198954.1      ATTACAGCAGGGGCCTTGGGCAAAGCACCCAGTAAAGTGACTGATGCCCTTCCAGAGCCA
NM_016079.3         ATTACAGCAGGGGCCTTGGGCAAAGCACCCAGTAAAGTGACTGATGCCCTTCCAGAGCCA
                    ************************************************************

HsCD00520982        GAACCTCCAGGAGCGATGGCTGCCTCAGAGGATGAGGAGGAGGAGGAAGAGGCTCTGGAG
NM_001198954.1      GAACCTCCAGGAGCGATGGCTGCCTCAGAGGATGAGGAGGAGGAGGAAGAGGCTCTGGAG
NM_016079.3         GAACCTCCAGGAGCGATGGCTGCCTCAGAGGATGAGGAGGAGGAGGAAGAGGCTCTGGAG
                    ************************************************************

HsCD00520982        GCCATGCAGTCCCGGCTGGCCACACTCCGCAGC---
NM_001198954.1      GCCATGCAGTCCCGGCTGGCCACACTCCGCAGCTAG
NM_016079.3         GCCATGCAGTCCCGGCTGGCCACACTCCGCAGCTAG
                    *********************************

Pathways

NCI :

Internalization of ErbB1

Reactome :

Budding and maturation of HIV virion

Reactome :

Disease

Reactome :

Endosomal Sorting Complex Required For Transport (ESCRT)

Reactome :

HIV Infection

Reactome :

HIV Life Cycle

Reactome :

Late Phase of HIV Life Cycle

Reactome :

Membrane Trafficking

Clone

Cloning Information :

582344

Original Clone ID :

WISP09-235

Organism

Species Specific ID:

51652

Genome

Chromosome :

Map Location :

Ensembl :

ENSG00000115561

Ensembl :

ENSG00000249884

Reagents

Labome :

CHMP3-antibody

CHMP3 (H. sapiens )In pCAG.MCS2.C-Myc (Myc-tagged mammalian expression vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Reference Sequence Alignment

Pathways

Clone

Organism

Genome

Reagents

CHMP3 (H. sapiens )
In pCAG.MCS2.C-Myc (Myc-tagged mammalian expression vector)