Gene:
Gene Symbol:
NDST3
Gene Name:
N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 3
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pGEn2-DEST
Format:
FUSION
Source:
Glyco-enzyme clone collection
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Georgia
Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
HsCD00522261
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AVAILABLE
No restriction
Special MTA: SPTA
Special MTA: SPTA
AGTGGCTACAAACAGGAAAATGAACTCTCTGAGACGGCTTCAGAAGTTGACTGTGGCGAC
CTCCAACACCTACCATATCAACTAATGGAAGTGAAAGCAATGAAGCTTTTTGATGCCTCA
AGGACAGACCCCACAGTCCTAGTATTTGTAGAGAGCCAGTACTCATCTCTTGGTCAAGAC
ATCATTATGATTCTAGAATCAAGTAGATTCCAGTATCACATTGAAATTGCCCCTGGAAAG
GGAGATCTCCCAGTGCTTATAGACAAAATGAAAGGCAAATACATTCTCATTATTTATGAG
AATATTTTAAAGTATATAAATATGGATTCCTGGAATCGAAGCCTTCTAGATAAATACTGT
GTAGAATATGGTGTGGGTGTCATTGGATTCCACAAAACTAGTGAGAAGAGTGTACAGAGC
TTTCAGTTAAAAGGTTTCCCTTTTTCCATATATGGAAATCTTGCAGTAAAAGATTGTTGT
ATTAATCCTCATTCTCCATTGATTCGTGTGACCAAATCTTCCAAGCTTGAAAAAGGTTCT
TTACCTGGAACTGACTGGACAGTTTTTCAGATTAATCATTCAGCCTATCAACCAGTAATA
TTTGCCAAAGTAAAGACCCCAGAAAACCTTTCTCCTTCCATCTCTAAAGGTGCTTTTTAT
GCCACTATTATACATGACCTGGGGCTTCATGATGGAATTCAAAGGGTTCTTTTTGGCAAC
AACTTGAACTTTTGGCTGCACAAGCTCATCTTCATAGATGCCATCTCCTTCTTATCAGGG
AAGAGGCTGACATTGTCCTTGGACAGGTACATTCTTGTGGATATTGATGATATATTTGTG
GGAAAAGAGGGAACAAGAATGAACACCAATGATGTAAAGGCCCTGCTTGATACTCAGAAT
CTTTTGCGTGCACAAATCACAAATTTTACATTCAACCTGGGATTTTCAGGGAAATTTTAC
CATACAGGAACTGAAGAGGAAGATGAAGGAGATGACTGTCTGTTGGGGTCTGTGGATGAG
TTCTGGTGGTTTCCTCACATGTGGAGCCATATGCAGCCCCACCTCTTCCACAATGAGTCA
TCTTTGGTGGAGCAGATGATTCTCAACAAAAAATTTGCCTTAGAGCACGGCATTCCAACG
GACATGGGCTACGCTGTGGCCCCTCACCATTCGGGCGTCTACCCTGTACATGTTCAGCTT
TATGAGGCCTGGAAGAAGGTCTGGAATATTAAAATCACCAGCACTGAAGAATATCCACAT
CTGAAGCCAGCTAGATACCGGAGGGGTTTTATCCACAAAAACATCATGGTTCTCCCAAGA
CAAACCTGTGGGCTTTTCACTCACACCATTTTCTACAAAGAATATCCAGGGGGTCCTAAA
GAGCTGGATAAGAGTATCCAAGGAGGAGAACTTTTCTTCACTGTCGTCCTCAACCCTATC
AGCATTTTCATGACCCATTTGTCCAACTATGGGAATGACCGACTGGGATTATATACATTT
GTTAATCTGGCCAACTTTGTGAAGAGCTGGACCAACCTGCGACTTCAGACTCTGCCTCCA
GTACAACTGGCCCACAAGTATTTTGAGCTGTTTCCTGATCAGAAAGACCCTCTCTGGCAG
AATCCTTGCGATGACAAACGCCACAGAGACATTTGGTCTAAAGAAAAAACTTGTGATCGC
TTACCAAAATTCTTGGTAATAGGACCCCAGAAAACTGGTACCACTGCTTTGTATTTGTTC
CTGGTTATGCATCCTTCCATCCTTAGTAACTCCCCCAGCCCAAAAACCTTTGAGGAGGTA
CAGTTCTTTAATAGAAATAACTACCACAGGGGGATTGATTGGTATATGGATTTCTTCCCA
GTCCCATCTAATGTCACTACCGACTTTTTGTTTGAGAAGAGTGCCAATTACTTCCACTCA
GAGGAAGCCCCTAAAAGAGCTGCTTCTCTGGTTCCCAAAGCCAAGATTATCACCATTCTC
ATTGACCCTTCAGACCGAGCATACTCCTGGTACCAGCATCAGCGATCACATGAAGACCCT
GCAGCTCTGAAGTTTAGCTTCTACGAAGTGATCTCAGCAGGGCCCCGTGCACCCTCGGAG
CTCAGAGCCTTGCAGAAGAGATGTTTGGTCCCGGGGTGGTATGCCAGCCACATCGAGAGA
TGGCTTGTTTATTTCCCCCCATTTCAGTTGCTAATTATTGATGGGCAACAACTAAGAACT
GATCCTGCTACAGTGATGGATGAAGTACAGAAGTTTCTAGGAGTCTTGCCTCATTATAAT
TACTCAGAAGCTTTAACGTTTGATTCTCATAAAGGTTTCTGGTGTCAGTTACTGGAAGAA
GGTAAAACAAAATGCCTTGGAAAGAGCAAAGGAAGAAAATACCCTCCAATGGATTCTGAT
AGCAGGACATTTCTGTCAAGCTACTATCGAGATCACAACGTGGAACTCTCAAAGCTGCTG
CACAAACTGGGTCAGCCTCTGCCATCCTGGCTGAGACAGGAGCTGCAGAAAGTAAGA
Protein sequence predicted based on nucleotide sequence
SGYKQENELSETASEVDCGDLQHLPYQLMEVKAMKLFDASRTDPTVLVFVESQYSSLGQD IIMILESSRFQYHIEIAPGKGDLPVLIDKMKGKYILIIYENILKYINMDSWNRSLLDKYC VEYGVGVIGFHKTSEKSVQSFQLKGFPFSIYGNLAVKDCCINPHSPLIRVTKSSKLEKGS LPGTDWTVFQINHSAYQPVIFAKVKTPENLSPSISKGAFYATIIHDLGLHDGIQRVLFGN NLNFWLHKLIFIDAISFLSGKRLTLSLDRYILVDIDDIFVGKEGTRMNTNDVKALLDTQN LLRAQITNFTFNLGFSGKFYHTGTEEEDEGDDCLLGSVDEFWWFPHMWSHMQPHLFHNES SLVEQMILNKKFALEHGIPTDMGYAVAPHHSGVYPVHVQLYEAWKKVWNIKITSTEEYPH LKPARYRRGFIHKNIMVLPRQTCGLFTHTIFYKEYPGGPKELDKSIQGGELFFTVVLNPI SIFMTHLSNYGNDRLGLYTFVNLANFVKSWTNLRLQTLPPVQLAHKYFELFPDQKDPLWQ NPCDDKRHRDIWSKEKTCDRLPKFLVIGPQKTGTTALYLFLVMHPSILSNSPSPKTFEEV QFFNRNNYHRGIDWYMDFFPVPSNVTTDFLFEKSANYFHSEEAPKRAASLVPKAKIITIL IDPSDRAYSWYQHQRSHEDPAALKFSFYEVISAGPRAPSELRALQKRCLVPGWYASHIER WLVYFPPFQLLIIDGQQLRTDPATVMDEVQKFLGVLPHYNYSEALTFDSHKGFWCQLLEE GKTKCLGKSKGRKYPPMDSDSRTFLSSYYRDHNVELSKLLHKLGQPLPSWLRQELQKVR
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
mammalian
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pGEn2-DEST
Synonyms:
pXLG-NtermTCMhisAviGfp-DEST
Sequencing Primer:
Forward:
pGEn2-DEST F
Reverse:
pGEn1-DEST R
Description:
Mammalian expression vector with a CMV promoter and N-terminal 8xHis, AviTag, and Super GFP tags; ampicillin resistance in bacteria; Gateway recombinational cloning
Comments:
None
Size (bp):
6930
Parent Vector:
None
Properties:
Gateway, acceptor (destination), fluorescent marker, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name:
University of Georgia
Kelley Moremen
Kelley Moremen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
Col E1 origin
1137
1804
death cassette
ccdB
ccdB death cassette (removed in plasmids with insert)
5072
5377
intron
artificial intron
artificial intron
2828
2960
poly-A signal
BGH pA
bovine growth hormone (BGH) polyadenylation signal
6106
6320
primer
forward
forward sequencing primer 5? AGCATGCACCACCACCATCACC 3?
3049
3070
primer
reverse
reverse sequencing primer 5' TGACAACGGGCCACAACTCCTC 3'
5729
5750
promoter
CMV
CMV promoter
2208
2790
recombination site
5'ITR
inverted terminal repeat sequence
1998
2127
recombination site
attR1
attR1 recombinational cloning site
3834
3962
recombination site
attR2
attR2 recombinational cloning site
5418
5542
recombination site
3'-ITR
inverted terminal repeat sequence
6408
6537
selectable marker
AmpR
ampicillin resistance
129
1108
selectable marker
CmR
Chloramphenicol resistance (CmR) gene (removed in plasmids with insert)
4071
4720
signal sequence
TCM
TCM signal sequence
2977
3052
tag
His
N-terminal 8xHis tag
3055
3078
tag
AviTag
AviTag
3079
3129
tag
GFP
N-terminal SuperGFP tag
3130
3825
trxn regulatory element
WPRE
woodchuck hepatitis post-transcriptional regulatory element
5558
6099
