DNASU Plasmid | TM0824 (T. maritima ) in pMH2T7 (bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: TM0824

Gene Name: astB/chuR-related protein

Original Clone ID:

8161

Keyword:

full-length cds

Species:

Thermotoga maritima

Type:

cDNA

Vector Name:

pMH2T7

Format:

CLOSED

Source:

Joint Center for Structural Genomics

Description:

None

Comments:

Linker not verified.

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

PSI
Joint Center for Structural Genomics

TmCD00530438

Price:

AVAILABLE

No restriction
Special MTA: PSI

Insert Sequence
Protein Sequence

Insert sequence: 1449nts

Open reading frame: 51 to 1499

GATCTGATAAAATTCATCATCATCATCATCACGTGGTCGACTCGAGCTTAATGGCAGTAG
GTGGAATCAAAGGAATGATCTATCGTCAGGCGGGAAAACTCGTTGGCTCATTTGTCAGAA
ACGCTGAAGTGGAAACTCTTGGAAAAATCTTCGGTACCCTGAGTATGTTCACGAAGGAGC
CCGCGAAGAGCGGCCTTCGAAAACTGGCTGACCTCGCAAAAGAAAGACATCCCATGGTGA
TGTCATGGGTGAACGTTTTCAAAAAAGCAAGTCCAAGGTGTGTTGAAGGGGTTATAAACA
ACCTGATCATCAACGAGTTCGCTCTCGGAGAACCTATCAGGCAGGAAAAGATGCACGAGT
ACAAAACGGTGCTCCCAAAACTCCTCGTTCTGAGCCCCACATACGCTTGTAATCTGAACT
GTGTTGGGTGCTATGCGGGACTTTACGGTAGAAAATACGAGCTCTCCCACGATGAAGTCA
GAGACATCTTGAAACAGGCCAACGACCTGGGAATATACTTCTTCATCATCACCGGTGGTG
AGCCCTTCTTCTGGCCTCACCTGATGGACATATTCGAAGAGTTCAAAGACAGCTACTTCC
TCGTTTACAGCAACGGAACTCTGATAAAAGAAGAAACCGCTAAGAAACTCGCAGAACTTG
GAAATGTGACGGTCTCCATATCTGTGGAAGGATTTGAAACCGACACAGACTGGAGAAGAG
GAAGGGGAATATTCAAGAGAGTCCTCGAAGCCTGGGAAAGGCTCAGAAGACATGGTGTGA
TCTTCGGAGCTTCTGTGACGGCAACACGAATGAACCACGATACCATAATGAAAGATGAAT
TCTGGGACTTTCTCGAAGAACAGGGAGTTTCTTACGTGTGGGTTTACCAGTTCATGCCCG
TGGGAATGAATCCAACGATGGACCTCGTTCCCACACCACAGCAGAGATACGAAAGATTCC
ACAAACTCGAACAGCTCAGACTCAGCGGAAGATTCGCTTTCGTTGCCGACTTCTGGAACC
ACGGGTTCCTCACCAATGGCTGTCTCGCAGCAGGTGCCAAGTACCTCCACATAAACGCGA
AAGGCTATGTAGAACCCTGTGTCTTCCAGCAGTTCGCGGTGGACAGCATTCGTGAGAAAA
AACTCATCGACATCTTGAGATCACCGTTCTTCGAAGCCTACAAGAGGATGATCCCTTACA
GCGACAATCTCTTCAGACCGTGTCCGATAATTGACAACCCGAAAGTCTTCAGAGCCATGG
TGAGAGCCTTCAACGCAAAACCACAGCACGAGGGATCCGAAAGGGTCATCACAGATCTCG
CGCCCGAACTGGACAAACTCGCAGCCGAATGGAAGAAATACGCAGACAAACTCTGGTACG
AAGAAGGTTACTCCGAGATCTATCCTGCCAACAGAGGAGTTTACAGCTACGAAGTGAGAA
TGAGGAGGTACGCAGACAAAGAAAAACTCCTCGCAGTGGATAAACGCTACAGGGACTAAG
TTTAAACGGTCTCCAGCTTGGCTGTTTTGGCGGATGAGAGAAGATTTTCA

Protein sequence predicted based on nucleotide sequence

MAVGGIKGMIYRQAGKLVGSFVRNAEVETLGKIFGTLSMFTKEPAKSGLRKLADLAKERH
PMVMSWVNVFKKASPRCVEGVINNLIINEFALGEPIRQEKMHEYKTVLPKLLVLSPTYAC
NLNCVGCYAGLYGRKYELSHDEVRDILKQANDLGIYFFIITGGEPFFWPHLMDIFEEFKD
SYFLVYSNGTLIKEETAKKLAELGNVTVSISVEGFETDTDWRRGRGIFKRVLEAWERLRR
HGVIFGASVTATRMNHDTIMKDEFWDFLEEQGVSYVWVYQFMPVGMNPTMDLVPTPQQRY
ERFHKLEQLRLSGRFAFVADFWNHGFLTNGCLAAGAKYLHINAKGYVEPCVFQQFAVDSI
REKKLIDILRSPFFEAYKRMIPYSDNLFRPCPIIDNPKVFRAMVRAFNAKPQHEGSERVI
TDLAPELDKLAAEWKKYADKLWYEEGYSEIYPANRGVYSYEVRMRRYADKEKLLAVDKRY
RD*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinExpressed: Protein_Confirmed

ProteinPurified: Protein_Purified

SolubleProtein: Protein_Soluble

Find experimental protocols in 282694

Recommended expression in:

Not Applicable

Vector Name:

pMH2T7

Synonyms:

pMH-2-T7

Sequencing Primer:

Forward: pBAD forward

Reverse: alt_bad_rev

Description:

Bacterial (E. coli) expression vector, arabinose or T7 induced expression, adds N-terminal MGSDKIHHHHHH tag; amp resistance; restriction enzyme (Pml I/Pac I) or PIPE cloning.

Comments:

None

Size (bp):

4084

Parent Vector:

None

Properties:

PIPE cloning, bacterial expression, in vitro transcription, inducible expression, with tag/fusion/marker

Author Name:

Joint Center for Structural Genomics

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

AAGAAACCAATTGTCCATATTGCATCAGACATTGCCGTCACTGCGTCTTTTACTGGCTCTTCTCGCTAACCAAACCGGTAACCCCGCTTATTAAAAGCATTCTGTAACAAAGCGGGACCAAAGCCATGACAAAAACGCGTAACAAAAGTGTCTATAATCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGCATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGTTTCTCCATACCCGTAATACGACTCACTATAGGGTTTTGTTTAACTTTAAGAAGGAGATATACATACCCATGGGATCTGATAAAATTCATCATCATCATCATCACGTGTAATTAATTAACTTTAAGAAGGAGATATACATACCCGGGTAAGTTTAAACGGTCTCCAGCTTGGCTGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTTTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCAGATCAATTCGCGCGCGAAGGCGAAGCGGCATGCATAATGTGCCTGTCAAATGGACGAAGCAGGGATTCTGCAAACCCTATGCTACTCCGTCAAGCCGTCAATTGTCTGATTCGTTACCAATTATGACAACTTGACGGCTACATCATTCACTTTTTCTTCACAACCGGCACGGAACTCGCTCGGGCTGGCCCCGGTGCATTTTTTAAATACCCGCGAGAAATAGAGTTGATCGTCAAAACCAACATTGCGACCGACGGTGGCGATAGGCATCCGGGTGGTGCTCAAAAGCAGCTTCGCCTGGCTGATACGTTGGTCCTCGCGCCAGCTTAAGACGCTAATCCCTAACTGCTGGCGGAAAAGATGTGACAGACGCGACGGCGACAAGCAAACATGCTGTGCGACGCTGGCGATATCAAAATTGCTGTCTGCCAGGTGATCGCTGATGTACTGACAAGCCTCGCGTACCCGATTATCCATCGGTGGATGGAGCGACTCGTTAATCGCTTCCATGCGCCGCAGTAACAATTGCTCAAGCAGATTTATCGCCAGCAGCTCCGAATAGCGCCCTTCCCCTTGCCCGGCGTTAATGATTTGCCCAAACAGGTCGCTGAAATGCGGCTGGTGCGCTTCATCCGGGCGAAAGAACCCCGTATTGGCAAATATTGACGGCCAGTTAAGCCATTCATGCCAGTAGGCGCGCGGACGAAAGTAAACCCACTGGTGATACCATTCGCGAGCCTCCGGATGACGACCGTAGTGATGAATCTCTCCTGGCGGGAACAGCAAAATATCACCCGGTCGGCAAACAAATTCTCGTCCCTGATTTTTCACCACCCCCTGACCGCGAATGGTGAGATTGAGAATATAACCTTTCATTCCCAGCGGTCGGTCGATAAAAAAATCGAGATAACCGTTGGCCTCAATCGGCGTTAAACCCGCCACCAGATGGGCATTAAACGAGTATCCCGGCAGCAGGGGATCATTTTGCGCTTCAGCCATACTTTTCATACTCCCGCCATTCAGAG

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

ColEI origin of replication

1921

2603

promoter

T7 promoter

290

310

promoter

araC

araC promoter

256

285

selectable marker

AmpR

ampicillin resistance gene

1164

1823

tag

His

N-terminal 6x His tag

367

381

trxn regulatory element

arabinose O1 operator

161

182

trxn regulatory element

arabinose O2 operator

Gene

Gene Symbol :

TM0824

GENEID :

898494

Locus Tag :

TM0824

GI :

15643587

GenBank Accession :

AAD35906

Target GenBank:

NP_228633

Protein

SMART domain :

Elp3 : Elongator protein 3, MiaB family, Radical SAM

Structural Annotation Wiki :

282694

UniProt :

Q9WZT1

Clone

Cloning Information :

276067

HIP Master Clone ID :

205553

Original Clone ID :

8161

Experimental

TargetTrack - Experimental data :

282694

Organism

Species Specific ID:

898494

Reagents

Labome :

TM0824-cDNA