Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
10356y3BCt8p1
Keyword:
None
Species:
Bacteroides thetaiotaomicron
Type:
cDNA
Vector Name:
pSGX3
Format:
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
Linker not verified.
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
New York SGX Research Center for Structural Genomics
BtCD00531742
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 2844nts
Open reading frame: 1 to 2844
TCAACACAAAGCGAAGCGGCATTGGAAGCCGGACTGATAGCTACACTCCGACAAATGGAC
TACGAGTATGTCCAGATTGTCGAGGAAGATAATCTTTATGCCAATTTCAAGCGGCAGTTG
GAGATTCACAACCGCAAACGATTGGAAGAGCACGGACGTACTGAGTTTACGGCAGAAGAG
TTTGAGAAGATTCTCATATACCTTGAAGGTGGTACTCGCTTTGAAAAGGCAAAGAAACTC
CGTGACTTATATCCGCTTGATACGGCTGATGGACAGCGTATATGGGTGGAATTTCTAAAT
AGGCAGCAGTGGTGTCAGAATGAATTTCAGGTTTCCAACCAGATTACGGTGGAAGGACGT
AAAAAATGCCGCTATGATGTGACGATTCTAATCAATGGTTTGCCACTTGTGCAGATTGAA
TTGAAACGTCGTGGCGTGGAACTTAAACAGGCATACAACCAAATACAGCGTTATCACAAG
ACTTCGTTTCACGGATTGTTTGATTATATCCAACTGTTTGTCATATCCAACGGTGTTAAT
ACTCGCTATTTTGCCAATAATCCAAATAGTGGGTATAAGTTCACATTCAACTGGACAGAT
GCTGCCAATCATCCGTTCAATGAATTGGATAAGTTTGCCGTATTCTTCTTGGAAAAATGT
ACGCTTGGCAAAATCATAGGAAAGTATATTGTGCTGCATGAGGGCGACAAGTGCTTGATG
GTACTTCGTCCTTACCAGTTCTATGCAGTGGAGAAGATTCTGGATCGTGTGCAGAACTCC
AACGATAACGGCTATATCTGGCATACTACAGGTGCAGGAAAGACATTGACTTCGTTCAAG
GCCGCACAACTTGTGTCAGAATTGGACGATGTGGATAAGGTTATGTTTGTTGTTGATCGA
CACGACCTTGATACCCAGACTCAATCGGAATACGAAGCGTTTGAACCAGGGGCTGTAGAT
AGTACTGATAATACGGATGAATTAGTGAAACGGCTTCAAAGCAATTCCAAAATTATCATA
ACCACCATTCAAAAACTTAATGCGGCAGTTAGTAAAACGTGGTATAGCAGCAAGATTGAT
GCTATCCGTCATTCACGTATCGTTATGATATTTGATGAGTGCCATCGCAGTCATTTTGGG
GATAGTCATAAGAAGATAATGAAGTTTTTTGGCAATGCCCAAATATTCGGTTTTACTGGA
ACACCTATCTTCACCGAGAATGCGGTGGATGGACATACCACTAAGGAAATATTCGGAAAC
TGTCTTCATAAATATCTTATTAAAGATGCAATAGCGGATGAAAATGTGCTTGGCTTCCTT
GTGGAGTATTATCACGGCAACGAAATAGTTGATAACGATAACCAAGCTCGTATGGAGGAG
ATTGCCAAATTTATCCTTAACAATTTTAACAAATCTACATTCGATGGAGAGTTTGATGCT
TTGTTTGCGGTGCAATCCGTGCCGATGCTTATACGCTATTACAAGATATTCAGATCGTTG
AATCCGAAAATCCGTATTGGTGCTGTGTTTACTTATGCTGCTAACAATAGTCAGGATGAT
GAACAGACCGGCATGGGTACAGGGCAGTATGCAAAAGAGAGCGTGGGTGAGGCGGATGAA
CTGCAAGCCATCATGGACGATTATAATGAAAACTTCGGCACATCTTTTACCACGGAGAAC
TTCCGTGCTTACTATGATGACATCAATCTGCGCATGAAAAAGAAGAAGGCGGATATGAAA
CCGCTTGATCTTTGTCTTGTTGTGGGTATGTTCCTTACAGGCTTTGACAGTAAAAAGCTG
AATACGCTCTATGTGGACAAGAATATGGAATATCATGGTTTGCTGCAAGCGTTCAGTCGA
ACCAACCGAGTGTTGAACGAGAAGAAACGTTTTGGTAAAATAGTTTGTTTCAGAGATTTG
AAAAACAATGTTGATACGTCTATTAAATTGTTCAGCAACTCTGATAATCCTGAAGATATA
GTACGCCCTCCGTTTGAAAAAGTAAAGCAGGAGTATAAGAGTTTGTCAACTGATTTTCTG
CAAAAATATCCAACACCAAGTAGTATAGACTTTCTGCAAAGCGAAAATGACAAGAAGAAT
TTTGTGCTTGCATTCCGTGATATTATCCGTAAACATGCAGAAATACAGATATACGAAGAT
TACAGCGAGGATGCTGAAGACCTCGGCATGACTGAACAACAGTTTAACGATTATAAGAGT
AAATATCTTGATATTACAGTCGGGTTTATTGACCCACCTGCTGCACCGTCAACTGTTGCG
GAAGATCCTGTTCCATACGGAAATAACCAAGGATTGGAAGATATTGATTTCTGTCTTGAG
CTCTTACACAGTGACATTATCAATGTGGCTTACATTCTTGCACTAATTAATGACCTTGAC
CCTTATAGTGATGATTATTCTGAAAAACGCCAGAATATCATTGATACAATGATTAAGGAT
GCCGGAATGCGTGGCAAAGCCAAACTCATTGACGGTTTTATCAAAAAGAATGTTGATGAA
GACAAGGAGAATTTCATGAACGGTCGTAATAAGGCTGACGGAACAAACGAATTGGAAGAA
CGATTAAACCAATATATTGTGTCAGAACGCAATAAGGCCGTGAATGATTTGGCTGATGAA
GAACAGATTTCAGCGGAGGTGCTTAATCATTACATGAAGGAGTATGACTATTTACAAAAA
GAGCAGCCGGAGATTATACAAAAGGCATTGAAAGAGAAACATCTTGGTCTTATCAAAACC
CGAAGGGCTTTGACGAGAATAATGGAAAGACTGCGTAGTATTATAAAAACGTTTAATGAA
GGGCATCATCACCATCACCACTGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGC
ACTCGAGCACCACCA
Protein sequence predicted based on nucleotide sequence
STQSEAALEAGLIATLRQMDYEYVQIVEEDNLYANFKRQLEIHNRKRLEEHGRTEFTAEE FEKILIYLEGGTRFEKAKKLRDLYPLDTADGQRIWVEFLNRQQWCQNEFQVSNQITVEGR KKCRYDVTILINGLPLVQIELKRRGVELKQAYNQIQRYHKTSFHGLFDYIQLFVISNGVN TRYFANNPNSGYKFTFNWTDAANHPFNELDKFAVFFLEKCTLGKIIGKYIVLHEGDKCLM VLRPYQFYAVEKILDRVQNSNDNGYIWHTTGAGKTLTSFKAAQLVSELDDVDKVMFVVDR HDLDTQTQSEYEAFEPGAVDSTDNTDELVKRLQSNSKIIITTIQKLNAAVSKTWYSSKID AIRHSRIVMIFDECHRSHFGDSHKKIMKFFGNAQIFGFTGTPIFTENAVDGHTTKEIFGN CLHKYLIKDAIADENVLGFLVEYYHGNEIVDNDNQARMEEIAKFILNNFNKSTFDGEFDA LFAVQSVPMLIRYYKIFRSLNPKIRIGAVFTYAANNSQDDEQTGMGTGQYAKESVGEADE LQAIMDDYNENFGTSFTTENFRAYYDDINLRMKKKKADMKPLDLCLVVGMFLTGFDSKKL NTLYVDKNMEYHGLLQAFSRTNRVLNEKKRFGKIVCFRDLKNNVDTSIKLFSNSDNPEDI VRPPFEKVKQEYKSLSTDFLQKYPTPSSIDFLQSENDKKNFVLAFRDIIRKHAEIQIYED YSEDAEDLGMTEQQFNDYKSKYLDITVGFIDPPAAPSTVAEDPVPYGNNQGLEDIDFCLE LLHSDIINVAYILALINDLDPYSDDYSEKRQNIIDTMIKDAGMRGKAKLIDGFIKKNVDE DKENFMNGRNKADGTNELEERLNQYIVSERNKAVNDLADEEQISAEVLNHYMKEYDYLQK EQPEIIQKALKEKHLGLIKTRRALTRIMERLRSIIKTFNEGHHHHHH*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in NYSGXRC-10356y
Recommended expression in:
Not Applicable
Vector Name:
pSGX3
Synonyms:
pSGX-3, pSGX3_BC
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Bacterial (E. coli) expression vector with T7 promoter, adds C-terminal 6xHis tag; kanamycin resistance; TOPO cloning.
Comments:
Modified from pET26b from Novagen
Size (bp):
5339
Parent Vector:
None
Properties:
TOPO Cloning, bacterial expression, in vitro transcription, with tag/fusion/marker
Author Name:
New York SGX Research Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
ATG start
start
initiation start (Met, Ser, Leu)
5072
5079
TOPO recognition site
TOPO
TOPO cloning site
5076
5085
bacterial origin
ori
pBR322-type bacterial origin of replication
1421
2103
gene fragment
ROP
ROP
2527
2703
promoter
T7
T7 promoter
4983
5003
repressor protein gene
LacI
LacI CDS
3515
4531
selectable marker
KanR
kanamycin resistance gene
563
1375
tag
His
6xHis tag
5086
5103
trxn regulatory element
lac operator
lac operator
5003
5025
trxn termination sequence
T7 term
T7 terminator
5231
5307
