SPy_1110 (S. pyogenes )
In pSGX3 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  SPy_1110
Gene Name:  NAD-dependent malic enzyme
Original Clone ID:
6108d1BCt6b1
Keyword:
full-length cds
Species:
Streptococcus pyogenes
Type:
cDNA
Vector Name:
pSGX3
Format: 
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
SpCD00533512
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1188nts
Open reading frame: 1 to 1188

AAAAATCAATTAGGTCAACTTGCCCTTGAACAAGCCAAAACGTTCGGCGGTAAATTGGAA
GTTCAACCAAAAGTTGACATTAAAACTAAACATGATTTGAGTATTGCCTACACACCAGGT
GTAGCGAGTGTGTCATCTGCCATCGCAAAAGATAAAACATTAGCATATGACTTAACCACC
AAGAAAAATACAGTAGCCGTTATCAGTGATGGTACTGCGGTTCTTGGTCTGGGGGATATT
GGCCCTGAAGCAGCCATGCCAGTTATGGAAGGAAAAGCAGCCCTTTTTAAAGCATTTGCG
GGTGTTGATGCTATTCCGATTGTATTAGATACCAAAGATACTGAAGAAATTATTAGTATT
GTCAAGGCGCTTGCACCAACCTTTGGTGGTATTAATTTAGAGGATATTAGTGCGCCCCGC
TGTTTTGAAATTGAGCAGCGCTTGATTAAGGAATGTCATATTCCTGTTTTCCATGACGAT
CAGCATGGAACAGCTATTGTGGTGTTAGCAGCCATCTTCAATAGTTTGAAACTTCTTAAA
AAATCCCTTGATGAGGTATCCATTGTGGTGAATGGTGGTGGATCTGCTGGATTATCCATT
ACCCGTAAATTATTAGCAGCAGGTGCTACGAAAGTGACAGTAGTGGATAAATTTGGCATT
ATCAATGAACAAGAAGCCGCTCAATTAGCGCCACATCATTTGGATATAGCGAAAGTCACT
AATCGTGAGTTTAAATCAGGTACCTTAGAAGATGCTCTTGAAGGAGCAGATATCTTCATT
GGCGTATCAGCACCAGGAGTCTTAAAAGCAGAGTGGATTAGTAAAATGGCAGCGCGCCCA
GTAATTTTTGCCATGGCCAACCCTATTCCAGAGATTTACCCTGATGAAGCCCTTGAAGCG
GGGGCTTACATTGTAGGTACTGGCCGCAGTGATTTTCCTAACCAGATTAATAATGTCCTT
GCCTTTCCAGGTATTTTTAGGGGAGCATTAGATGCACGGGCAAAAACCATTACTGTTGAG
ATGCAAATTGCTGCCGCAAAAGGTATTGCCAGTCTTGTCCCAGATGACGCTTTGAGCACT
ACTAATATTATTCCAGATGCCTTTAAAGAAGGAGTCGCTGAGATTGTCGCTAAAAGCGTC
AGGTCGGTAGTCCTTAAATCAGAAGGGCATCATCACCATCACCACTGA

Protein sequence predicted based on nucleotide sequence

KNQLGQLALEQAKTFGGKLEVQPKVDIKTKHDLSIAYTPGVASVSSAIAKDKTLAYDLTT
KKNTVAVISDGTAVLGLGDIGPEAAMPVMEGKAALFKAFAGVDAIPIVLDTKDTEEIISI
VKALAPTFGGINLEDISAPRCFEIEQRLIKECHIPVFHDDQHGTAIVVLAAIFNSLKLLK
KSLDEVSIVVNGGGSAGLSITRKLLAAGATKVTVVDKFGIINEQEAAQLAPHHLDIAKVT
NREFKSGTLEDALEGADIFIGVSAPGVLKAEWISKMAARPVIFAMANPIPEIYPDEALEA
GAYIVGTGRSDFPNQINNVLAFPGIFRGALDARAKTITVEMQIAAAKGIASLVPDDALST
TNIIPDAFKEGVAEIVAKSVRSVVLKSEGHHHHHH*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in NYSGXRC-6108d
Recommended expression in:
Not Applicable