Gene:
Gene Symbol:
NUP188
Gene Name:
Nup188p
Original Clone ID:
15188a47BCt13p1
Keyword:
None
Species:
Saccharomyces cerevisiae
Type:
cDNA
Vector Name:
pSGX3
Format:
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
Linker not verified.
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
New York SGX Research Center for Structural Genomics
ScCD00533639
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 3117nts
Open reading frame: 22 to 3138
GGAGATATACATATGTCCCTTATGAACGACGCAGCAGCAGACGTGAGCGCAGTGGACGCA
AAGCAGCTGGCACAGATCCGTCAGTTCCTGAAGGCCAACAAGACCAACCTGATCGAAAGC
CTGAACACCATCCGTCAGAACGTGACCAGCAGCGGTGACCACAACAAGCTGCGTAGCACC
ATCGCCAACCTGCTGCAGATCAACGTGGACAACGACCCGTTCTTCGCCCAGAGCGAAGAC
CTGAGCCACGCCGTGGAATTTTTCATGAGCGAACGTAGCAGCCGTCTGCACATCGTGTAC
AGCCTGCTGGTGAACCCGGACATCGACCTGGAAACCTACAGCTTCATCGACAACGACCGT
TTCAACGTGGTGGGCAAGCTGATCAGCATCATCAGCAGCGTGATCCAGAACTACGACATC
ATCACCGCCAGCAGCCTGGCCCACGACTACAACAACGACCAGGACATGTTCACCATCGTG
AGCCTGGTGCAGCTGAAGAAGTTCAGCGACCTGAAGTTCATCCTGCAGATCCTGCAGATC
CTGAACCTGATGATCCTGAACACCAAGGTGCCGGTGGACATCGTGAACCAGTGGTTCCTG
CAGTACCAGAACCAGTTCGTGGAGTTCTGCCGTAACATCAACAGCACCGACAAGAGCATC
GACACCAGCAGCCTGCAGCTGTACAAGTTCCAGAACTTCCAGGACCTGAGCTACCTGAGC
GAAACCCTGATCAGCCGTATCAGCAGCCTGTTCACCATCACCACCATCCTGATCCTGGGT
CTGAACACCAGCATCGCCCAGTTCGACATCCAGAGCCCGCTGTACATGGACACCGAAACC
TTCGACACCGTGAACAGCGCCCTGGAAAACGACGTGGCCACCAACATCGTGAACGAAGAC
CCGATCTTCCACCCGATGATCCACTACAGCTGGAGCTTCATCCTGTACTACCGTCGTGCC
CTGCAGAGCAGCGAATCCTTCGACGACAGCGACATCACCAAGTTCGCCCTGTTCGCCGAA
AGCCACGACGTGCTGCAGAAGCTGAACACCCTGAGCGAAATCCTGAGCTTCGACCCGGTG
TACACCACCGTGATCACCGTGTTCCTGGAATTTAGCCTGAACTTCATCCCGATCACCGCC
AGCACCAGCCGTGTGTTCGCCAAGATCATCAGCAAGGCCCCGGAACAGTTCATCGAAAAC
TTCCTGACCAACGACACCTTCGAAAAGAAGCTGAGCATCATCAAGGCCAAGCTGCCGCTG
CTGAACGAAAGCCTGATCCCGCTGATCAACCTGGCCCTGATCGACACCGAGTTCGCCAAC
TTCGAACTGAAGGACATCTGCAGCTTCGCCGTGACCAAGAGCAGCCTGAACGACCTGGAC
TACGACCTGATCGCCGACACCATCACCAACTCTTCCTCAAGTAGCGACATCATCGTGCCG
GACCTGATCGAACTGAAGAGCGACCTGCTGGTGGCCCCGCCGCTGGAAAACGAAAACAGC
AACTGCCTGCTGAGCATCCCGAAGAGCACCAAGGGCAAGATCCTGACCATCAAGCAACAA
CAGCAGCAGCAGCAGAACGGTCAGCAGCCGCCGACCACCAGCAACCTGATCATCTTCCTG
TACAAGTTCAACGGTTGGAGCCTGGTGGGTCGTATCCTGCAGAACCTGCTGCACAGCTAC
ATGGAAAAGGGTACCCAGCTGGACGACCTGCAGCACGAACTGATGATCAGCATCATCAAG
CTGGTGACCAACGTGGTGGACCCGAAGACCAGCATCGAAAAGAGCAGCGAAATCCTGAGC
TACCTGAGCAACAGCCTGGACACCAGCGCCAGCACCATCAACGGTGCCAGCATCATCCAG
GTGATCTTCGAAATCTTCGAAATCAGCCTGCAGCGTAAGGACTACACCAGCATCGTGCAG
TGCTGCGAGTTCATGACCATGCTGACCCCGAACTACCTGCACCTTGTGAGCAGCTACCTG
AACAAGAGCGACCTGCTGGACAAGTACGGCAAGACCGGTCTGAGCAACATGATCCTGGGT
AGCGTGGAACTGAGCACCGGTGACTACACCTTCACCATCCAGCTGCTGAAGCTGACCAAG
GTGTTCATCCGTGAAAGCCTGAGCCTGAAGAACATCCACATCAGCAAGCGTAGCAAGATC
GACATCATCAACAAGCTGATCCTGCACGCCATCCACATCTTCGAAAGCTACTACAACTGG
AAGTACAACAACTTCCTGCAGAAGTTCGAAATCGCCTTCCACCTGACCCTGATCTTCTAC
GACGTGCTGCACGACGTGTTCACCATCAACCCGCATCAGAAGGACCAGCTGATCATCTCT
TCCAGCGCCAACAAGCTGCTGCAGCTGTTCCTGACCCCGATGGACAGCATCGACCTGGCC
CCGAACACCCTGACCAACATCCTGATCAGCCCGCTGAACACCACCACCAAGATCCTGGGT
GACAAGATCCTGGGTAACCTGTACAGCAAGGTGATGAACAACAGCTTCAAGCTGTGCACC
CTGCTGATCAGCATCCGTGGTAGCAACCGTGACCTGAAGCCGAGCAACCTGGAAAAGCTG
CTGTTCATCAACAGCAGCAAGCTGGTGGACGTGTACACCCTGCCGAGCTACGTGCACTTC
AAGGTGCAGATCATCGAACTGCTGAGCTACCTGGTGGAAGCCCCGTGGAACGACGACTAC
CCGTTCCTGCTGAGCTTCCTGGGTGAAGCCAAGAGCATGGCCTTCCTGAAGGAAGTGCTG
AGCGACCTGAGCAGCCCGGTGCAGGACTGGAACCTGCTGCGTAGCCTGTACATCTTCTTC
ACCACCCTGCTGGAAAGCAAGCAGGACGGTCTGAGCATCCTGTTCCTGACCGGCCAGTTC
GCCAGCAACAAGAAGATCAATGACGAAAGCAGCATCGACAAGAAGAGCAGCATCCTGACC
GTGCTGCAGAAGAACAGCCTGCTGCTGGACAGCACCCCGGAAGAAGTGAGCTGCAAGCTG
CTGGAAACCATCACCTACGTGCTGAACACCTGGACCAACAGCAAGATCTTCATCAAGGAC
CCGAAGTTCGTGAACAGCCTGCTGGCCAAGCTGAAGGACAGCAAGAAGCTGGAAGGGCAT
CATCACCATCACCACTGATCCGAATTCGAGCTCCG
Protein sequence predicted based on nucleotide sequence
MNDAAADVSAVDAKQLAQIRQFLKANKTNLIESLNTIRQNVTSSGDHNKLRSTIANLLQI NVDNDPFFAQSEDLSHAVEFFMSERSSRLHIVYSLLVNPDIDLETYSFIDNDRFNVVGKL ISIISSVIQNYDIITASSLAHDYNNDQDMFTIVSLVQLKKFSDLKFILQILQILNLMILN TKVPVDIVNQWFLQYQNQFVEFCRNINSTDKSIDTSSLQLYKFQNFQDLSYLSETLISRI SSLFTITTILILGLNTSIAQFDIQSPLYMDTETFDTVNSALENDVATNIVNEDPIFHPMI HYSWSFILYYRRALQSSESFDDSDITKFALFAESHDVLQKLNTLSEILSFDPVYTTVITV FLEFSLNFIPITASTSRVFAKIISKAPEQFIENFLTNDTFEKKLSIIKAKLPLLNESLIP LINLALIDTEFANFELKDICSFAVTKSSLNDLDYDLIADTITNSSSSSDIIVPDLIELKS DLLVAPPLENENSNCLLSIPKSTKGKILTIKQQQQQQQNGQQPPTTSNLIIFLYKFNGWS LVGRILQNLLHSYMEKGTQLDDLQHELMISIIKLVTNVVDPKTSIEKSSEILSYLSNSLD TSASTINGASIIQVIFEIFEISLQRKDYTSIVQCCEFMTMLTPNYLHLVSSYLNKSDLLD KYGKTGLSNMILGSVELSTGDYTFTIQLLKLTKVFIRESLSLKNIHISKRSKIDIINKLI LHAIHIFESYYNWKYNNFLQKFEIAFHLTLIFYDVLHDVFTINPHQKDQLIISSSANKLL QLFLTPMDSIDLAPNTLTNILISPLNTTTKILGDKILGNLYSKVMNNSFKLCTLLISIRG SNRDLKPSNLEKLLFINSSKLVDVYTLPSYVHFKVQIIELLSYLVEAPWNDDYPFLLSFL GEAKSMAFLKEVLSDLSSPVQDWNLLRSLYIFFTTLLESKQDGLSILFLTGQFASNKKIN DESSIDKKSSILTVLQKNSLLLDSTPEEVSCKLLETITYVLNTWTNSKIFIKDPKFVNSL LAKLKDSKKLEGHHHHHH*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
del@540,1
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in NYSGXRC-15188a
Recommended expression in:
Not Applicable
Vector Name:
pSGX3
Synonyms:
pSGX-3, pSGX3_BC
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Bacterial (E. coli) expression vector with T7 promoter, adds C-terminal 6xHis tag; kanamycin resistance; TOPO cloning.
Comments:
Modified from pET26b from Novagen
Size (bp):
5339
Parent Vector:
None
Properties:
TOPO Cloning, bacterial expression, in vitro transcription, with tag/fusion/marker
Author Name:
New York SGX Research Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
ATG start
start
initiation start (Met, Ser, Leu)
5072
5079
TOPO recognition site
TOPO
TOPO cloning site
5076
5085
bacterial origin
ori
pBR322-type bacterial origin of replication
1421
2103
gene fragment
ROP
ROP
2527
2703
promoter
T7
T7 promoter
4983
5003
repressor protein gene
LacI
LacI CDS
3515
4531
selectable marker
KanR
kanamycin resistance gene
563
1375
tag
His
6xHis tag
5086
5103
trxn regulatory element
lac operator
lac operator
5003
5025
trxn termination sequence
T7 term
T7 terminator
5231
5307
