Gene:
Gene Symbol:
NUP188
Gene Name:
Nup188p
Original Clone ID:
15188a71BCt13p1
Keyword:
None
Species:
Saccharomyces cerevisiae
Type:
cDNA
Vector Name:
pSGX3
Format:
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
Linker not verified.
Mutation / Discrepancy:
Yes / Yes
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
New York SGX Research Center for Structural Genomics
ScCD00533643
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 2934nts
Open reading frame: 39 to 2972
TTTGTTTACTTTAAGAAGGAGATATACATATGTCCCTTAACAGCCTGGACACCAGCGCCA
GCACCATCAACGGTGCCAGCATCATCCAGGTGATCTTCGAAATCTTCGAAATCAGCCTGC
AGCGTAAGGACTACACCAGCATCGTGCAGTGCTGCGAGTTCATGACCATGCTGACCCCGA
ACTACCTGCACCTTGTGAGCAGCTACCTGAACAAGAGCGACCTGCTGGACAAGTACGGCA
AGACCGGTCTGAGCAACATGATCCTGGGTAGCGTGGAACTGAGCACCGGTGACTACACCT
TCACCATCCAGCTGCTGAAGCTGACCAAGGTGTTCATCCGTGAAAGCCTGAGCCTGAAGA
ACATCCACATCAGCAAGCGTAGCAAGATCGACATCATCAACAAGCTGATCCTGCACGCCA
TCCACATCTTCGAAAGCTACTACAACTGGAAGTACAACAACTTCCTGCAGAAGTTCGAAA
TCGCCTTCCACCTGACCCTGATCTTCTACGACGTGCTGCACGACGTGTTCACCATCAACC
CGCATCAGAAGGACCAGCTGATCATCTCTTCCAGCGCCAACAAGCTGCTGCAGCTGTTCC
TGACCCCGATGGACAGCATCGACCTGGCCCCGAACACCCTGACCAACATCCTGATCAGCC
CGCTGAACACCACCACCAAGATCCTGGGTGACAAGATCCTGGGTAACCTGTACAGCAAGG
TGATGAACAACAGCTTCAAGCTGTGCACCCTGCTGATCAGCATCCGTGGTAGCAACCGTG
ACCTGAAGCCGAGCAACCTGGAAAAGCTGCTGTTCATCAACAGCAGCAAGCTGGTGGACG
TGTACACCCTGCCGAGCTACGTGCACTTCAAGGTGCAGATCATCGAACTGCTGAGCTACC
TGGTGGAAGCCCCGTGGAACGACGACTACCCGTTCCTGCTGAGCTTCCTGGGTGAAGCCA
AGAGCATGGCCTTCCTGAAGGAAGTGCTGAGCGACCTGAGCAGCCCGGTGCAGGACTGGA
ACCTGCTGCGTAGCCTGTACATCTTCTTCACCACCCTGCTGGAAAGCAAGCAGGACGGTC
TGAGCATCCTGTTCCTGACCGGCCAGTTCGCCAGCAACAAGAAGATCAATGACGAAAGCA
GCATCGACAAGAAGAGCAGCATCCTGACCGTGCTGCAGAAGAACAGCCTGCTGCTGGACA
GCACCCCGGAAGAAGTGAGCTGCAAGCTGCTGGAAACCATCACCTACGTGCTGAACACCT
GGACCAACAGCAAGATCTTCATCAAGGACCCGAAGTTCGTGAACAGCCTGCTGGCCAAGC
TGAAGGACAGCAAGAAGCTGTTCCAGAAGAAGGAAAACCTGACCCGTGACGAAACCGTGA
GCCTGATCAAGAAGTACAAGCTGATCAGCCGTATCGTGGAAATCTTCGCCCTGTGCATCT
ACAACAGCACCGACAGCAACAGCGAAATCCTGAACTTCCTGAACCAGGAAGACCTGTTCG
AACTGGTGCATCACTTCTTCCAGATCGACGGTTTCAACAAGACCTTCCACGACGAACTGA
ACCTGAAGTTCAAGGAAAAGTGGCCGAGCCTGGAACTGCAGAGCTTCCAGAAGATCCCGC
TGAGCCGTATCAACGAAAACGAAAACTTCGGTTACGACATCCCTCTGCTGGACATCGTGC
TGAAGGCCGACCGTAGCTGGAACGAACCGAGCAAGAGCCAGACCAACTTCAAGGAAGAAA
TCACCGACGCCAGCCTGAACCTGCAGTACGTGAACTACGAAATCAGCACCGCCAAGGCCT
GGGGTGCCCTGATCACCACCTTCGTGAAGCGTAGCACCGTGCCGCTGAACGACGGTTTCG
TGGACCTGGTGGAACACTTCCTGAAGCTGAACATCGACTTCGGTAGCGACAAGCAGATGT
TCACCCAGATCTACCTGGAACGTATCGAACTGAGCTTCTACATCCTGTACAGCTTCAAGC
TGAGCGGCAAGCTGCTGAAGGAAGAAAAGATCATCGAACTGATGAACAAGATCTTCACCA
TCTTCAAGAGCGGTGAAATCGACTTCATCAAGAACATCGGCAAGAGCCTGAAGAACAACT
TCTACCGTCCGCTGCTGCGTAGCGTGCTGGTGCTGCTGGAACTTGTGAGCAGCGGTGACC
GTTTCATCGAACTGATCAGCGACCAGCTGCTGGAATTTTTCGAACTGGTGTTCAGCAAGG
GTGTGTACCTGATCCTGAGCGAAATCCTGTGCCAGATCAACAAGTGCAGCACCCGTGGTC
TGAGCACCGACCACACCACCCAGATCGTGAACCTGGAAGACAACACCCAGGACCTGCTGC
TGCTGCTGAGCCTGTTCAAGAAGATCACCAACCTGAACCCGAGCAAGAACTTCAACGTGA
TCCTGGCCAGCAGCCTGAACGAAGTGGGTACCCTGAAGGTGATCCTGAACCTGTACAGCA
GCGCCCACCTGATCCGTATCAACGACGAACCGATCCTGGGTCAGATCACCCTGACCTTCA
TCAGCGAACTGTGCAGCATCGAACCGATCGCCGCCAAGCTGATCAACAGCGGTCTGTACA
GCGTGCTGCTGGAAAGCCCGCTGAGCGTGGCCATCCAGCAGGGTGACATCAAGCCGGAGT
TCAGCCCGCGTCTGCACAACATCTGGAGCAACGGTCTGCTGAGCATCGTTCTGCTGCTGC
TGTCTCAGTTCGGTATCAAGGTGCTGCCGGAAACCTGCCTGTTCGTGAGCTACTTCGGCA
AGCAGATCAAGAGCACCATCTACAACTGGGGTGACAACAAGCTGGCCGTGTCTTCCAGCC
TGATCAAGGAAACCAACCAGCTGGTGCTGCTGCAGAAGATGCTGAACCTGCTGAACTACC
AGGAACTGTTCATCCAGCCGAAGAACAGCGACGACCAGCAGGAAGCCGTGGAACTGGTGA
TCGGTGAAGGGCATCATCACCATCACCACTGATCCGAATTCGAGCTCC
Protein sequence predicted based on nucleotide sequence
NSLDTSASTINGASIIQVIFEIFEISLQRKDYTSIVQCCEFMTMLTPNYLHLVSSYLNKS DLLDKYGKTGLSNMILGSVELSTGDYTFTIQLLKLTKVFIRESLSLKNIHISKRSKIDII NKLILHAIHIFESYYNWKYNNFLQKFEIAFHLTLIFYDVLHDVFTINPHQKDQLIISSSA NKLLQLFLTPMDSIDLAPNTLTNILISPLNTTTKILGDKILGNLYSKVMNNSFKLCTLLI SIRGSNRDLKPSNLEKLLFINSSKLVDVYTLPSYVHFKVQIIELLSYLVEAPWNDDYPFL LSFLGEAKSMAFLKEVLSDLSSPVQDWNLLRSLYIFFTTLLESKQDGLSILFLTGQFASN KKINDESSIDKKSSILTVLQKNSLLLDSTPEEVSCKLLETITYVLNTWTNSKIFIKDPKF VNSLLAKLKDSKKLFQKKENLTRDETVSLIKKYKLISRIVEIFALCIYNSTDSNSEILNF LNQEDLFELVHHFFQIDGFNKTFHDELNLKFKEKWPSLELQSFQKIPLSRINENENFGYD IPLLDIVLKADRSWNEPSKSQTNFKEEITDASLNLQYVNYEISTAKAWGALITTFVKRST VPLNDGFVDLVEHFLKLNIDFGSDKQMFTQIYLERIELSFYILYSFKLSGKLLKEEKIIE LMNKIFTIFKSGEIDFIKNIGKSLKNNFYRPLLRSVLVLLELVSSGDRFIELISDQLLEF FELVFSKGVYLILSEILCQINKCSTRGLSTDHTTQIVNLEDNTQDLLLLLSLFKKITNLN PSKNFNVILASSLNEVGTLKVILNLYSSAHLIRINDEPILGQITLTFISELCSIEPIAAK LINSGLYSVLLESPLSVAIQQGDIKPEFSPRLHNIWSNGLLSIVLLLLSQFGIKVLPETC LFVSYFGKQIKSTIYNWGDNKLAVSSSLIKETNQLVLLQKMLNLLNYQELFIQPKNSDDQ QEAVELVIGEGHHHHHH*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
A860S; V1398L
Discrepancy:
g1626t,P542P
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in NYSGXRC-15188a
Recommended expression in:
Not Applicable
Vector Name:
pSGX3
Synonyms:
pSGX-3, pSGX3_BC
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Bacterial (E. coli) expression vector with T7 promoter, adds C-terminal 6xHis tag; kanamycin resistance; TOPO cloning.
Comments:
Modified from pET26b from Novagen
Size (bp):
5339
Parent Vector:
None
Properties:
TOPO Cloning, bacterial expression, in vitro transcription, with tag/fusion/marker
Author Name:
New York SGX Research Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
ATG start
start
initiation start (Met, Ser, Leu)
5072
5079
TOPO recognition site
TOPO
TOPO cloning site
5076
5085
bacterial origin
ori
pBR322-type bacterial origin of replication
1421
2103
gene fragment
ROP
ROP
2527
2703
promoter
T7
T7 promoter
4983
5003
repressor protein gene
LacI
LacI CDS
3515
4531
selectable marker
KanR
kanamycin resistance gene
563
1375
tag
His
6xHis tag
5086
5103
trxn regulatory element
lac operator
lac operator
5003
5025
trxn termination sequence
T7 term
T7 terminator
5231
5307
