DNASU Plasmid | DVU2210 (D. vulgaris str. Hildenborough )<br>In pSGX3 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: DVU2210

Gene Name: hypothetical protein

Original Clone ID:

10993d2BCt7p1

Keyword:

full-length cds

Species:

Desulfovibrio vulgaris str. Hildenborough

Type:

cDNA

Vector Name:

pSGX3

Format:

CLOSED

Source:

New York SGX Research Center for Structural Genomics

Description:

None

Comments:

Linker not verified.

Mutation / Discrepancy:

No / Yes

Publications:

None

Authors:

PSI
New York SGX Research Center for Structural Genomics

DvCD00537079

Price:

AVAILABLE

No restriction
Special MTA: PSI

Insert Sequence
Protein Sequence

Insert sequence: 1743nts

Open reading frame: 19 to 1761

GATATACATATGTCCCTTATACAGCTTCGCCCCTGCCCCAAGAGCTACACACGCGACCAG
GACAAGGCGACCACCCCGGCAGAGACCGTACGCCGGGTGCGCGAACGCCTCGCCTCGCTC
GACATCAGCATACTCGCCGAGACGCGCCGGGTGGATGTGGGACGCCTCGGCATCCCCGTC
TTTCTCAGCGTGTGCGGCGCCGATGCCCGTGCCGTGATGCCCACGCGCAAGCAGATGGGC
AAGGGTGCCTCGCTGGAACAGGCGCAGGCATCTGCGCTCATGGAGTTGATGGAGCGTTAC
AGCTTCTTCACCTTCTGGCGAGACATGCCCGGCATGGTGGAGGCCACATGGTCCGAAGCC
CGAAACCGTTTCGGCGACGCGCTGATACCCGTCGATGAGATTCTGCACTCCGTGCATGAC
ACCCTTTCCCCCGACGACGCCGTCCGCGCCCTCGACCTGTGGCGCTGGAAGTTCTTCCCC
GCCACGGACATCACCCACGGGCGCGAGGTATGGCTGCCCCTCGACTGGTTCAAGAAGCTT
GGTGAGTTCAACGGCTCATCGGCAGGCAACACCGAAGAGGAGTCCATCCTTCAAGGCAGT
TGCGAACTTGTGGAACGCCACGTGTGCTGCCTCATCGACCGCACACAGCCCGAGTTGCCC
ACCATCGACCCGGCGTCCCTCGACGACCCTGTGCTGCTTGAACTTGCGGCTGCCTTCGAG
CGCAACGGCATCCGGCTGGTGCTCAAGGACTTCTCCATGGGGATGCCCGTGCCTACGGTC
GGGGCCATCGCATGGGACCCTGCCACCTTCCCCGAACGCTCCGAAATCGTCTACACGGCA
GGCACGTCATCCTCTCCGGTGAAGGCAGCCATACGCGCCGTGACAGAGGTCGCACAGCTT
GCGGGCGACTTCTGCACCGGGGCCTGCTACGAGGCCTCGGGGCTTTCCAAATACGAAAGC
CTCGACGACATCGCGTGGCTTGTCGCCGGACCCGTTGTCGCCCTTGACACCCTGCCTTCG
GTGGAGACCGACGACATCCGCGACGAACTTGCCGCGCTCTGCGACGGGCTTGCCGCGACG
GGGCACACGCTCTACAGCGTGTCCACCATGAACCCCGATGTCGGCGTCAACACTCATTAC
AACATCGTGCCCGGGTTCCACTTCCGTGAACGCGACCGTAACGCCTCACTGGGGCTGTTC
ATCGGGCGCATCCTGAGTGAAGAGGCAGACGCCGGCACGGCGCTGGCGGGGCTCTCGGTT
CTGGAGGAGCTCTACCCCGGTGCGCATTTCCTGCCCTTCTTCAAGGGGATGCTGGCACTC
AGGGCCGAGTCGCACCACGAGGCCATCAGCTTCTTCGAACAGGCCGAACCGCTGCAGCCT
GAAGCGGACTCTCGCGGTCTGGCGGCCTTCTACGGTGCCTATGTGCGTACCCTGCTCACA
GACTGGCAAGGGGCCCTTCCCGGGCTGGACCGTGCCGTGACCGAATGCCCCGAGATGAAA
GAGTACTTCAACCTGCGCGGGGTGTGCCATTTCAAGCTTGGCGACTACGCACAGGCCGCA
CAGAACTTCGCGCAGGTACAGCATATCGACAAGGGGGCCGTGGTCGACCTCGCCAATCTC
GGCCTGTGCCACAAGTTCATGGGTGACACCGAGAAGGCCGAAGAGCTTCTCTCCGCCGCA
CTGGAGATAGACCCCGGCCTCGACTTCGCACGCCGCCATCTGGATGAGATGCGCGAAGGG
CATCATCACCATCACCACTGAGATAGACCCCGGCCTCGACTTCGCACGCCGCCATCTGGA
TGAGATGCGCGA

Protein sequence predicted based on nucleotide sequence

IQLRPCPKSYTRDQDKATTPAETVRRVRERLASLDISILAETRRVDVGRLGIPVFLSVCG
ADARAVMPTRKQMGKGASLEQAQASALMELMERYSFFTFWRDMPGMVEATWSEARNRFGD
ALIPVDEILHSVHDTLSPDDAVRALDLWRWKFFPATDITHGREVWLPLDWFKKLGEFNGS
SAGNTEEESILQGSCELVERHVCCLIDRTQPELPTIDPASLDDPVLLELAAAFERNGIRL
VLKDFSMGMPVPTVGAIAWDPATFPERSEIVYTAGTSSSPVKAAIRAVTEVAQLAGDFCT
GACYEASGLSKYESLDDIAWLVAGPVVALDTLPSVETDDIRDELAALCDGLAATGHTLYS
VSTMNPDVGVNTHYNIVPGFHFRERDRNASLGLFIGRILSEEADAGTALAGLSVLEELYP
GAHFLPFFKGMLALRAESHHEAISFFEQAEPLQPEADSRGLAAFYGAYVRTLLTDWQGAL
PGLDRAVTECPEMKEYFNLRGVCHFKLGDYAQAAQNFAQVQHIDKGAVVDLANLGLCHKF
MGDTEKAEELLSAALEIDPGLDFARRHLDEMREGHHHHHH*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

t1630a;L553Q

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: kanamycin

Bacterial Selection Condition: 50ug/mL kanamycin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.

Protein Expression Results:

ProteinExpressed: Protein_Confirmed

SolubleProtein: Protein_Soluble

ProteinPurified: Tested_Not_Purified

Find experimental protocols in NYSGXRC-10993d

Recommended expression in:

Not Applicable

Vector Name:

pSGX3

Synonyms:

pSGX-3, pSGX3_BC

Sequencing Primer:

Forward: T7

Reverse: T7 terminator

Description:

Bacterial (E. coli) expression vector with T7 promoter, adds C-terminal 6xHis tag; kanamycin resistance; TOPO cloning.

Comments:

Modified from pET26b from Novagen

Size (bp):

5339

Parent Vector:

None

Properties:

TOPO Cloning, bacterial expression, in vitro transcription, with tag/fusion/marker

Author Name:

New York SGX Research Center for Structural Genomics

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

TGGCGAATGGGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGTTTACAATTTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAATTAATTCTTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGTTTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGAGCGAGACGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGAGTAACCATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACAATCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCCTAGAGCAAGACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCTGCGGTAAAGCTCATCAGCGTGGTCGTGAAGCGATTCACAGATGTCTGCCTGTTCATCCGCGTCCAGCTCGTTGAGTTTCTCCAGAAGCGTTAATGTCTGGCTTCTGATAAAGCGGGCCATGTTAAGGGCGGTTTTTTCCTGTTTGGTCACTGATGCCTCCGTGTAAGGGGGATTTCTGTTCATGGGGGTAATGATACCGATGAAACGAGAGAGGATGCTCACGATACGGGTTACTGATGATGAACATGCCCGGTTACTGGAACGTTGTGAGGGTAAACAACTGGCGGTATGGATGCGGCGGGACCAGAGAAAAATCACTCAGGGTCAATGCCAGCGCTTCGTTAATACAGATGTAGGTGTTCCACAGGGTAGCCAGCAGCATCCTGCGATGCAGATCCGGAACATAATGGTGCAGGGCGCTGACTTCCGCGTTTCCAGACTTTACGAAACACGGAAACCGAAGACCATTCATGTTGTTGCTCAGGTCGCAGACGTTTTGCAGCAGCAGTCGCTTCACGTTCGCTCGCGTATCGGTGATTCATTCTGCTAACCAGTAAGGCAACCCCGCCAGCCTAGCCGGGTCCTCAACGACAGGAGCACGATCATGCGCACCCGTGGGGCCGCCATGCCGGCGATAATGGCCTGCTTCTCGCCGAAACGTTTGGTGGCGGGACCAGTGACGAAGGCTTGAGCGAGGGCGTGCAAGATTCCGAATACCGCAAGCGACAGGCCGATCATCGTCGCGCTCCAGCGAAAGCGGTCCTCGCCGAAAATGACCCAGAGCGCTGCCGGCACCTGTCCTACGAGTTGCATGATAAAGAAGACAGTCATAAGTGCGGCGACGATAGTCATGCCCCGCGCCCACCGGAAGGAGCTGACTGGGTTGAAGGCTCTCAAGGGCATCGGTCGAGATCCCGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGCTGTCTTCGGTATCGTCGTATCCCACTACCGAGATATCCGCACCAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCCCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCCCGTTCCGCTATCGGCTGAATTTGATTGCGAGTGAGATATTTATGCCAGCCAGCCAGACGCAGACGCGCCGAGACAGAACTTAATGGGCCCGCTAACAGCGCGATTTGCTGGTGACCCAATGCGACCAGATGCTCCACGCCCAGTCGCGTACCGTCTTCATGGGAGAAAATAATACTGTTGATGGGTGTCTGGTCAGAGACATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGATCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTACCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCCGGGATCTCGACGCTCTCCCTTATGCGACTCCTGCATTAGGAAGCAGCCCAGTAGTAGGTTGAGGCCGTTGAGCACCGCCGCCGCAAGGAATGGTGCATGCAAGGAGATGGCGCCCAACAGTCCCCCGGCCACGGGGCCTGCCACCATACCCACGCCGAAACAAGCGCTCATGAGCCCGAAGTGGCGAGCCCGATCTTCCCCATCGGTGATGTCGGCGATATAGGCGCCAGCAACCGCACCTGTGGCGCCGGTGATGCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGTCCCTTAAGGGCATCATCACCATCACCACTGATCCCACCACCACCACCACCACTGAGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGCACCACCACCACCACCACTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATCCGGAT

Vector Features:

Type

Name

Description

Start

End

ATG start

start

initiation start (Met, Ser, Leu)

5072

5079

TOPO recognition site

TOPO

TOPO cloning site

5076

5085

bacterial origin

ori

pBR322-type bacterial origin of replication

1421

2103

gene fragment

ROP

2527

2703

promoter

T7 promoter

4983

5003

repressor protein gene

LacI

LacI CDS

3515

4531

selectable marker

KanR

kanamycin resistance gene

563

1375

tag

His

6xHis tag

5086

5103

trxn regulatory element

lac operator

5003

5025

trxn termination sequence

T7 term

T7 terminator

5231

5307

Gene

Gene Symbol :

DVU2210

GENEID :

2795357

Locus Tag :

DVU2210

GI :

81830405

GenBank Accession :

Q729Y7

Target GenBank:

AE017285

Protein

SMART domain :

TPR : Tetratricopeptide repeats

Structural Annotation Wiki :

NYSGXRC-10993d

Clone

Cloning Information :

487927

HIP Master Clone ID :

402912

Original Clone ID :

10993d2BCt7p1

Experimental

TargetTrack - Experimental data :

NYSGXRC-10993d

Organism

Species Specific ID:

2795357

Reagents

Labome :

DVU2210-cDNA

DVU2210 (D. vulgaris str. Hildenborough )In pSGX3 (His-tagged bacterial expression vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Protein

Clone

Experimental

Organism

Reagents

DVU2210 (D. vulgaris str. Hildenborough )
In pSGX3 (His-tagged bacterial expression vector)