Gene:
Gene Symbol:
I8R
Gene Name:
temporal expression: early/intermediate
Original Clone ID:
VV077-3
Keyword:
RNA helicase; 1 aa change
Species:
Vaccinia virus WR
Type:
cDNA
Vector Name:
pDONR222
Format:
FUSION
Source:
University of Utah
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Utah
Nancy Villa
Nancy Villa
VvCD00543608
Price:
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AVAILABLE
No restriction
Special MTA: SPTA
Special MTA: SPTA
Insert sequence: 2028nts
Open reading frame: 1 to 2028
ATGGAAAAGAATTTACCAGATATCTTCTTTTTTCCAAACTGCGTTAATGTATTCTCTTAC
AAATATTCACAAGATGAATTCAGTAATATGAGTAAAACGGAACGTGATAGTTTCTCATTG
GCCGTGTTTCCAGTTATAAAACATAGATGGCATAACGCACACGTTGTAAAACATAAAGGA
ATATACAAAGTTAGTACAGAAGCACGTGGAAAAAAAGTATCTCCTCCATCACTAGGAAAA
CCCGCACACATAAACCTAACCGCGAAGCAATATATATACAGTGAACACACAATAAGCTTT
GAATGTTATAGTTTTCTAAAATGTATAACAAATACAGAAATCAATTCGTTCGATGAGTAT
ATATTAAGAGGACTATTAGAAGCTGGTAATAGTTTACAGATATTTTCCAATTCCGTAGGT
AAACGAACAGATACTATAGGTGTACTAGGGAATAAGTATCCATTTAGCAAAATTCCATTG
GCCTCATTAACTCCTAAAGCACAACGAGAGATATTTTCAGCGTGGATTTCTCATAGACCT
GTAGTTTTAACTGGAGGAACTGGAGTGGGTAAGACGTCACAGGTACCCAAGTTATTGCTT
TGGTTTAATTATTTATTTGGTGGATTCTCTACTCTAGATAAAATCACTAACTTTCACGAA
AGACCAGTCATTCTATCTCTTCCTAGGATAGCTTTAGTTAGATTGCATAGCAATACCATT
TTAAAATCATTGGGATTTAAGGTACTAGATGGATCTCCTATTTCTTTACGGTACGGATCT
ATACCGGAAGAATTAATAAACAAACAACCAAAAAAATATGGAATTGTATTTTCTACCCAT
AAGTTATCTCTAACAAAACTATTTAGTTATGGCACTCTTATTATAGACGAAGTTCATGAG
CATGATCAAATAGGAGATATTATTATAGCAGTAGCGAGAAAGCATCATACGAAAATAGAT
TCTATGTTTTTAATGACTGCCACGTTAGAGGATGACCGAGAACGGCTAAAAGTATTTTTA
CCTAATCCCGCATTTATACATATTCCTGGAGATACACTGTTTAAAATTAGCGAGGTATTT
ATTCATAATAAGATAAATCCATCTTCCAGAATGGCATACATAGAAGAAGAAAAGAGAAAT
TTAGTTACTGCTATACAGATGTATACTCCTCCTGATGGATCATCCGGTATAGTCTTTGTG
GCATCCGTTGCACAGTGTCACGAATATAAATCATATTTAGAAAAAAGATTACCGTATGAT
ATGTATATTATTCATGGTAAGGTCTTAGATATAGACGAAATATTAGAAAAAGTGTATTCA
TCACCTAATGTATCGATAATTATTTCTACTCCTTATTTGGAATCCAGCGTTACTATACGC
AATGTTACACACATTTATGATATGGGTAAAGTTTTTGTCCCCGCTCCTTTTGGAGGATCG
CAAGAATTTATTTCTAAATCTATGAGAGATCAACGAAAAGGAAGAGTAGGAAGAGTTAAT
CCTGGTACATACGTCTATTTCTATGATCTGTCTTATATGAAGTCTATACAGCGAATAGAT
TCAGAATTTCTACATAATTATATATTGTACGCTAATAAGTTTAATCTAACACTCCCCGAA
GATTTGTTTATAATCCCTACAAATTTGGATATTCTATGGCGTACAAAGGAATATATAGAC
TCGTTCGATATTAGTACAGAAACATGGAATAAATTATTATCCAATTATTATATGAAGATG
ATAGAGTATGCTAAACTTTATGTACTAAGTCCTATTCTCGCTGAGGAGTTGGATAACTTT
GAGAGGACGGGAGAATTAACTAGTATTGTACGAGAAGCCATTTTATCTCTAAATTTACGA
ATTAAGATTTTAAATTTTAAACATAAAGATGATGATACGTATATACACTTTTGTAAAATA
TTATTCGGTGTCTATAACGGAACAAACGCTACTATATATTATCATAGACCTCTAACGGGA
TATATGAATATGATTTCAGATACTATATTTGTTCCTGTAGATAATAAC
Protein sequence predicted based on nucleotide sequence
MEKNLPDIFFFPNCVNVFSYKYSQDEFSNMSKTERDSFSLAVFPVIKHRWHNAHVVKHKG IYKVSTEARGKKVSPPSLGKPAHINLTAKQYIYSEHTISFECYSFLKCITNTEINSFDEY ILRGLLEAGNSLQIFSNSVGKRTDTIGVLGNKYPFSKIPLASLTPKAQREIFSAWISHRP VVLTGGTGVGKTSQVPKLLLWFNYLFGGFSTLDKITNFHERPVILSLPRIALVRLHSNTI LKSLGFKVLDGSPISLRYGSIPEELINKQPKKYGIVFSTHKLSLTKLFSYGTLIIDEVHE HDQIGDIIIAVARKHHTKIDSMFLMTATLEDDRERLKVFLPNPAFIHIPGDTLFKISEVF IHNKINPSSRMAYIEEEKRNLVTAIQMYTPPDGSSGIVFVASVAQCHEYKSYLEKRLPYD MYIIHGKVLDIDEILEKVYSSPNVSIIISTPYLESSVTIRNVTHIYDMGKVFVPAPFGGS QEFISKSMRDQRKGRVGRVNPGTYVYFYDLSYMKSIQRIDSEFLHNYILYANKFNLTLPE DLFIIPTNLDILWRTKEYIDSFDISTETWNKLLSNYYMKMIEYAKLYVLSPILAEELDNF ERTGELTSIVREAILSLNLRIKILNFKHKDDDTYIHFCKILFGVYNGTNATIYYHRPLTG YMNMISDTIFVPVDNN
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR222
Synonyms:
''
Sequencing Primer:
Forward:
Unknown
Reverse:
Unknown
Description:
Gateway®, donor (entry), recombinational cloning clone with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and TetR and ori; kanamycin resistance in bacterial;
Comments:
None
Size (bp):
4718
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
2899
3714
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
987
1292
CDS
CmR
confers resistance to chloramphenicol
1612
2295
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
58
85
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
177
263
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
327
343
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
2816
2832
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2296
2398
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
2793
2811
promoter
tetR and tetA promoters
overlapping promoters for bacterial tetR and tetA
3722
3777
protein binding site
attP1
recombination site for the Gateway BP reaction
360
591
protein binding site
attP2
recombination site for the Gateway BP reaction
2543
2774
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4104
4692
