DNASU Plasmid | RRM2B (Homo sapiens) in pSpeedE5T (Bacterial Expression)

Explanation of Terms

Gene:

Gene Symbol: RRM2B

Gene Name: ribonucleotide reductase M2 B (TP53 inducible)

Original Clone ID:

99289

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pSpeedE5T

Format:

CLOSED

Source:

Joint Center for Structural Genomics

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

PSI:Biology
Joint Center for Structural Genomics

HsCD00545621

Price:

AVAILABLE

No restriction
Special MTA: PSI

Insert Sequence
Protein Sequence

Insert sequence: 1056nts

Open reading frame: 51 to 1106

Reference Sequence Alignment

GATCTGATAAAATTCATCATCATCATCACGAAAACCTGTACTTCCAGGGCATGGGCGACC
CGGAAAGGCCGGAAGCGGCCGGGCTGGATCAGGATGAGAGATCATCTTCAGACACCAACG
AAAGTGAAATAAAGTCAAATGAAGAGCCACTCCTAAGAAAGAGTTCTCGCCGGTTTGTCA
TCTTTCCAATCCAGTACCCTGATATTTGGAAAATGTATAAACAGGCACAGGCTTCCTTCT
GGACAGCAGAAGAGGTCGACTTATCAAAGGATCTCCCTCACTGGAACAAGCTTAAAGCAG
ATGAGAAGTACTTCATCTCTCACATCTTAGCCTTTTTTGCAGCCAGTGATGGAATTGTAA
ATGAAAATTTGGTGGAGCGCTTTAGTCAGGAGGTGCAGGTTCCAGAGGCTCGCTGTTTCT
ATGGCTTTCAAATTCTCATCGAGAATGTTCACTCAGAGATGTACAGTTTGCTGATAGACA
CTTACATCAGAGATCCCAAGAAAAGGGAATTTTTATTTAATGCAATTGAAACCATGCCCT
ATGTTAAGAAAAAAGCAGATTGGGCCTTGCGATGGATAGCAGATAGAAAATCTACTTTTG
GGGAAAGAGTGGTGGCCTTTGCTGCTGTAGAAGGAGTTTTCTTCTCAGGATCTTTTGCTG
CTATATTCTGGCTAAAGAAGAGAGGTCTTATGCCAGGACTCACTTTTTCCAATGAACTCA
TCAGCAGAGATGAAGGACTTCACTGTGACTTTGCTTGCCTGATGTTCCAATACTTAGTAA
ATAAGCCTTCAGAAGAAAGGGTCAGGGAGATCATTGTTGATGCTGTCAAAATTGAGCAGG
AGTTTTTAACAGAAGCCTTGCCAGTTGGCCTCATTGGAATGAATTGCATTTTGATGAAAC
AGTACATTGAGTTTGTAGCTGACAGATTACTTGTGGAACTTGGATTCTCAAAGGTTTTTC
AGGCAGAAAATCCTTTTGATTTTATGGAAAACATTTCTTTAGAAGGAAAAACAAATTTCT
TTGAGAAACGAGTTTCAGAGTATCAGCGTTTTGCAGTTATGGCAGAAACCACAGATAACG
TCTTCACCTTGGATGCAGATTTTTAACGCGACTTAATTAACTCGTTTAAACGGTCTCCAG
CTTGGCTGTTTTGGCGG

Protein sequence predicted based on nucleotide sequence

MGDPERPEAAGLDQDERSSSDTNESEIKSNEEPLLRKSSRRFVIFPIQYPDIWKMYKQAQ
ASFWTAEEVDLSKDLPHWNKLKADEKYFISHILAFFAASDGIVNENLVERFSQEVQVPEA
RCFYGFQILIENVHSEMYSLLIDTYIRDPKKREFLFNAIETMPYVKKKADWALRWIADRK
STFGERVVAFAAVEGVFFSGSFAAIFWLKKRGLMPGLTFSNELISRDEGLHCDFACLMFQ
YLVNKPSEERVREIIVDAVKIEQEFLTEALPVGLIGMNCILMKQYIEFVADRLLVELGFS
KVFQAENPFDFMENISLEGKTNFFEKRVSEYQRFAVMAETTDNVFTLDADF*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: kanamycin

Bacterial Selection Condition: 50ug/mL kanamycin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.

Protein Expression Results:

ProteinExpressed: Protein_Confirmed

ProteinPurified: Protein_Purified

SolubleProtein: Protein_Soluble

Find experimental protocols in 400263

Recommended expression in:

Not Applicable

Vector Name:

pSpeedE5T

Synonyms:

Sequencing Primer:

Forward: pBAD forward

Reverse: alt_bad_rev

Description:

Bacterial expression vector with a pBAD/T7 promoter and TEV-cleavable N-terminal 6xHis tag; arabinose-inducible; kanamycin resistance in bacteria; PIPE cloning

Comments:

None

Size (bp):

4058

Parent Vector:

None

Properties:

PIPE cloning, bacterial expression, with tag/fusion/marker

Author Name:

Joint Center for Structural Genomics
PSI:Biology

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

Not Available

Vector Features:

Type

Name

Description

Start

End

death cassette

ccdB

ccdB death cassette (not present in plasmids containing gene inserts)

403

1808

primer

pBADfwd

pBAD forward sequencing primer

208

227

primer

pBADrev

pBAD reverse sequencing primer

1909

1926

promoter

T7 promoter

291

307

promoter

pBAD

pBAD promoter

250

277

protease cleavage site

TEV

TEV protease cleavage site

382

402

repressor protein gene

araC

AraC ORF

3157

4032

selectable marker

KanR

kanamycin resistance

2396

3211

tag

His

N-terminal 6xHis tag MGSDKIHHHHHENLYFQG

346

402

trxn regulatory element

arabinose O1 operator

arabinose O1 operator region

trxn regulatory element

arabinose O2 operator

arabinose O2 operator region

161

182

Gene

Gene Symbol :

RRM2B

Symbol Nomenclature :

RRM2B

SYNONYM :

MTDPS8A; MTDPS8B; P53R2

Designation :

TP53-inducible ribonucleotide reductase M2 B|p53-inducible ribonucleotide reductase small subunit 2 homolog|p53-inducible ribonucleotide reductase small subunit 2 short form beta|p53-inducible ribonucleotide reductase small subunit 2-like protein|ribonucleoside-diphosphate reductase subunit M2 B

Full Nomenclature :

ribonucleotide reductase M2 B (TP53 inducible)

GENEID :

50484

GI :

42544136

GenBank Accession :

NM_015713

HGNC :

17296

MIM :

604712

Vega :

OTTHUMG00000164776

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00545621        ------------------------------------------------------------
NM_015713.4         ------------------------------------------------------------
NM_001172477.1      ATGTTGCTGTTGCGTCTTCCCCCTCACCGCAGTCACGCCAGCCCGTTAGATTGCAAGTTG
                                                                                

HsCD00545621        ------------------------------------------------------------
NM_015713.4         ------------------------------------------------------------
NM_001172477.1      CAGGACCGCTGTAGGAAATGTTATTCGCCGCGGTCAGGACAGGCCTGTCCGCCCGCCCTC
                                                                                

HsCD00545621        ----------------------------------------------GATCTGATAAAATT
NM_015713.4         ------------------------------------------------------------
NM_001172477.1      GCCGCAGCCTGGCTTCGTCGTTGCGAGCGCCGGGGAGGCCGTCCCCGGGGAGGGCGGAGG
                                                                                

HsCD00545621        CATCATCATCATCACGAAAACCTGTACTTCCAGGGCATGGGCGACCCGGAAAGGCCGGAA
NM_015713.4         ------------------------------------ATGGGCGACCCGGAAAGGCCGGAA
NM_001172477.1      AAGGAGCTGACTTTGGGTTTGCGTCCCGCTCGCTGCTCTGCCCCGGGGCCAGCCAAGGAC
                                                        :  * * .   * .*.  ..***.

HsCD00545621        GCGGCCGGGCTGGATCAGGATGAGAGATCATCTTCAGACACCAACGAAAGTGAAATAAAG
NM_015713.4         GCGGCCGGGCTGGATCAGGATGAGAGATCATCTTCAGACACCAACGAAAGTGAAATAAAG
NM_001172477.1      GACGCTTGGAGGCCTCAGGCCGGGAGATCATCTTCAGACACCAACGAAAGTGAAATAAAG
                    *. **  **. * .*****. *.*************************************

HsCD00545621        TCAAATGAAGAGCCACTCCTAAGAAAGAGTTCTCGCCGGTTTGTCATCTTTCCAATCCAG
NM_015713.4         TCAAATGAAGAGCCACTCCTAAGAAAGAGTTCTCGCCGGTTTGTCATCTTTCCAATCCAG
NM_001172477.1      TCAAATGAAGAGCCACTCCTAAGAAAGAGTTCTCGCCGGTTTGTCATCTTTCCAATCCAG
                    ************************************************************

HsCD00545621        TACCCTGATATTTGGAAAATGTATAAACAGGCACAGGCTTCCTTCTGGACAGCAGAAGAG
NM_015713.4         TACCCTGATATTTGGAAAATGTATAAACAGGCACAGGCTTCCTTCTGGACAGCAGAAGAG
NM_001172477.1      TACCCTGATATTTGGAAAATGTATAAACAGGCACAGGCTTCCTTCTGGACAGCAGAAGAG
                    ************************************************************

HsCD00545621        GTCGACTTATCAAAGGATCTCCCTCACTGGAACAAGCTTAAAGCAGATGAGAAGTACTTC
NM_015713.4         GTCGACTTATCAAAGGATCTCCCTCACTGGAACAAGCTTAAAGCAGATGAGAAGTACTTC
NM_001172477.1      GTCGACTTATCAAAGGATCTCCCTCACTGGAACAAGCTTAAAGCAGATGAGAAGTACTTC
                    ************************************************************

HsCD00545621        ATCTCTCACATCTTAGCCTTTTTTGCAGCCAGTGATGGAATTGTAAATGAAAATTTGGTG
NM_015713.4         ATCTCTCACATCTTAGCCTTTTTTGCAGCCAGTGATGGAATTGTAAATGAAAATTTGGTG
NM_001172477.1      ATCTCTCACATCTTAGCCTTTTTTGCAGCCAGTGATGGAATTGTAAATGAAAATTTGGTG
                    ************************************************************

HsCD00545621        GAGCGCTTTAGTCAGGAGGTGCAGGTTCCAGAGGCTCGCTGTTTCTATGGCTTTCAAATT
NM_015713.4         GAGCGCTTTAGTCAGGAGGTGCAGGTTCCAGAGGCTCGCTGTTTCTATGGCTTTCAAATT
NM_001172477.1      GAGCGCTTTAGTCAGGAGGTGCAGGTTCCAGAGGCTCGCTGTTTCTATGGCTTTCAAATT
                    ************************************************************

HsCD00545621        CTCATCGAGAATGTTCACTCAGAGATGTACAGTTTGCTGATAGACACTTACATCAGAGAT
NM_015713.4         CTCATCGAGAATGTTCACTCAGAGATGTACAGTTTGCTGATAGACACTTACATCAGAGAT
NM_001172477.1      CTCATCGAGAATGTTCACTCAGAGATGTACAGTTTGCTGATAGACACTTACATCAGAGAT
                    ************************************************************

HsCD00545621        CCCAAGAAAAGGGAATTTTTATTTAATGCAATTGAAACCATGCCCTATGTTAAGAAAAAA
NM_015713.4         CCCAAGAAAAGGGAATTTTTATTTAATGCAATTGAAACCATGCCCTATGTTAAGAAAAAA
NM_001172477.1      CCCAAGAAAAGGGAATTTTTATTTAATGCAATTGAAACCATGCCCTATGTTAAGAAAAAA
                    ************************************************************

HsCD00545621        GCAGATTGGGCCTTGCGATGGATAGCAGATAGAAAATCTACTTTTGGGGAAAGAGTGGTG
NM_015713.4         GCAGATTGGGCCTTGCGATGGATAGCAGATAGAAAATCTACTTTTGGGGAAAGAGTGGTG
NM_001172477.1      GCAGATTGGGCCTTGCGATGGATAGCAGATAGAAAATCTACTTTTGGGGAAAGAGTGGTG
                    ************************************************************

HsCD00545621        GCCTTTGCTGCTGTAGAAGGAGTTTTCTTCTCAGGATCTTTTGCTGCTATATTCTGGCTA
NM_015713.4         GCCTTTGCTGCTGTAGAAGGAGTTTTCTTCTCAGGATCTTTTGCTGCTATATTCTGGCTA
NM_001172477.1      GCCTTTGCTGCTGTAGAAGGAGTTTTCTTCTCAGGATCTTTTGCTGCTATATTCTGGCTA
                    ************************************************************

HsCD00545621        AAGAAGAGAGGTCTTATGCCAGGACTCACTTTTTCCAATGAACTCATCAGCAGAGATGAA
NM_015713.4         AAGAAGAGAGGTCTTATGCCAGGACTCACTTTTTCCAATGAACTCATCAGCAGAGATGAA
NM_001172477.1      AAGAAGAGAGGTCTTATGCCAGGACTCACTTTTTCCAATGAACTCATCAGCAGAGATGAA
                    ************************************************************

HsCD00545621        GGACTTCACTGTGACTTTGCTTGCCTGATGTTCCAATACTTAGTAAATAAGCCTTCAGAA
NM_015713.4         GGACTTCACTGTGACTTTGCTTGCCTGATGTTCCAATACTTAGTAAATAAGCCTTCAGAA
NM_001172477.1      GGACTTCACTGTGACTTTGCTTGCCTGATGTTCCAATACTTAGTAAATAAGCCTTCAGAA
                    ************************************************************

HsCD00545621        GAAAGGGTCAGGGAGATCATTGTTGATGCTGTCAAAATTGAGCAGGAGTTTTTAACAGAA
NM_015713.4         GAAAGGGTCAGGGAGATCATTGTTGATGCTGTCAAAATTGAGCAGGAGTTTTTAACAGAA
NM_001172477.1      GAAAGGGTCAGGGAGATCATTGTTGATGCTGTCAAAATTGAGCAGGAGTTTTTAACAGAA
                    ************************************************************

HsCD00545621        GCCTTGCCAGTTGGCCTCATTGGAATGAATTGCATTTTGATGAAACAGTACATTGAGTTT
NM_015713.4         GCCTTGCCAGTTGGCCTCATTGGAATGAATTGCATTTTGATGAAACAGTACATTGAGTTT
NM_001172477.1      GCCTTGCCAGTTGGCCTCATTGGAATGAATTGCATTTTGATGAAACAGTACATTGAGTTT
                    ************************************************************

HsCD00545621        GTAGCTGACAGATTACTTGTGGAACTTGGATTCTCAAAGGTTTTTCAGGCAGAAAATCCT
NM_015713.4         GTAGCTGACAGATTACTTGTGGAACTTGGATTCTCAAAGGTTTTTCAGGCAGAAAATCCT
NM_001172477.1      GTAGCTGACAGATTACTTGTGGAACTTGGATTCTCAAAGGTTTTTCAGGCAGAAAATCCT
                    ************************************************************

HsCD00545621        TTTGATTTTATGGAAAACATTTCTTTAGAAGGAAAAACAAATTTCTTTGAGAAACGAGTT
NM_015713.4         TTTGATTTTATGGAAAACATTTCTTTAGAAGGAAAAACAAATTTCTTTGAGAAACGAGTT
NM_001172477.1      TTTGATTTTATGGAAAACATTTCTTTAGAAGGAAAAACAAATTTCTTTGAGAAACGAGTT
                    ************************************************************

HsCD00545621        TCAGAGTATCAGCGTTTTGCAGTTATGGCAGAAACCACAGATAACGTCTTCACCTTGGAT
NM_015713.4         TCAGAGTATCAGCGTTTTGCAGTTATGGCAGAAACCACAGATAACGTCTTCACCTTGGAT
NM_001172477.1      TCAGAGTATCAGCGTTTTGCAGTTATGGCAGAAACCACAGATAACGTCTTCACCTTGGAT
                    ************************************************************

HsCD00545621        GCAGATTTTTAACGCGACTTAATTAACTCGTTTAAACGGTCTCCAGCTTGGCTGTTTTGG
NM_015713.4         GCAGATTTTTAA------------------------------------------------
NM_001172477.1      GCAGATTTTTAA------------------------------------------------
                    ************                                                

HsCD00545621        CGG
NM_015713.4         ---
NM_001172477.1      ---

Pathways

NCI :

Direct p53 effectors

Panther :

De novo purine biosynthesis

Panther :

De novo pyrimidine deoxyribonucleotide biosynthesis

Reactome :

Metabolism

Reactome :

Metabolism of nucleotides

Reactome :

Synthesis and interconversion of nucleotide di- and triphosphates

WikiPathway :

DNA damage response

WikiPathway :

miRNA regulation of DNA Damage Response

WikiPathway :

Nucleotide Metabolism

Protein

Structural Annotation Wiki :

400263

HPRD :

05276

Clone

Cloning Information :

622007

Original Clone ID :

99289

Experimental

TargetTrack - Experimental data :

400263

Organism

Species Specific ID:

50484

Genome

Chromosome :

Map Location :

8q23.1

Ensembl :

ENSG00000048392

Reagents

Labome :

p53R2-antibody