DNASU Plasmid | SMARCA5 (Homo sapiens) in pSpeedET (bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: SMARCA5

Gene Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 5

Original Clone ID:

85582

Keyword:

partial cds

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pSpeedET

Format:

CLOSED

Source:

Joint Center for Structural Genomics

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

PSI:Biology
Joint Center for Structural Genomics

HsCD00545919

Price:

AVAILABLE

No restriction
Special MTA: PSI

Insert Sequence
Protein Sequence

Insert sequence: 1566nts

Open reading frame: 51 to 1616

Reference Sequence Alignment

CTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGCATGAAACCAG
GGCGCCCACGAATAAAAAAAGATGAGAAGCAGAACTTACTATCCGTTGGCGATTACCGAC
ACCGTAGAACAGAGCAAGAGGAGGATGAAGAGCTATTAACAGAAAGCTCCAAAGCAACCA
ATGTTTGCACTCGATTTGAAGACTCTCCATCGTATGTAAAATGGGGTAAACTGAGAGATT
ATCAGGTCCGAGGATTAAACTGGCTCATTTCTTTGTATGAGAATGGCATCAATGGTATCC
TTGCAGATGAAATGGGCCTAGGAAAGACTCTTCAAACAATTTCTCTTCTTGGGTACATGA
AACATTATAGAAACATTCCTGGGCCTCATATGGTTTTGGTTCCTAAGTCTACATTACACA
ACTGGATGAGTGAATTCAAGAGATGGGTACCAACACTTAGATCTGTTTGTTTGATAGGAG
ATAAAGAACAAAGAGCTGCTTTTGTCAGAGACGTTTTATTACCGGGAGAATGGGATGTAT
GTGTAACATCTTATGAAATGCTTATTAAAGAGAAGTCTGTGTTCAAAAAATTTAATTGGA
GATACTTAGTAATAGATGAAGCTCACAGGATCAAAAATGAAAAATCTAAGTTGTCAGAAA
TAGTGAGGGAATTCAAGACTACAAATAGACTATTATTAACTGGAACACCTCTTCAGAACA
ACTTGCATGAGCTGTGGTCACTTCTTAACTTTCTGTTGCCAGATGTGTTTAATTCAGCAG
ATGACTTTGATTCCTGGTTTGATACAAACAACTGCCTTGGGGATCAAAAACTAGTTGAGA
GGCTTCATATGGTTTTGCGTCCATTCCTCCTTCGTCGAATTAAGGCTGATGTTGAAAAGA
GTTTGCCTCCAAAGAAGGAAGTAAAAATCTATGTGGGCCTCAGCAAAATGCAAAGGGAAT
GGTATACTCGGATATTAATGAAGGATATAGATATACTCAACTCAGCAGGCAAGATGGACA
AAATGAGGTTATTGAACATCCTAATGCAGTTGAGAAAATGTTGTAATCATCCATATCTCT
TTGATGGAGCAGAACCTGGTCCACCTTATACAACAGATATGCATCTAGTAACCAACAGTG
GCAAAATGGTGGTTTTAGACAAGCTGCTCCCTAAGTTAAAAGAACAAGGTTCACGAGTAC
TAATCTTCAGTCAAATGACAAGGGTATTGGACATTTTGGAAGATTATTGCATGTGGAGAA
ATTATGAGTACTGCAGGTTGGATGGTCAGACACCCCATGATGAGAGACAAGACTCCATCA
ATGCATACAATGAACCAAACAGCACAAAGTTTGTTTTCATGTTAAGCACGCGTGCTGGTG
GTCTTGGCATCAATCTTGCGACTGCTGATGTAGTAATTTTGTATGATTCTGATTGGAATC
CCCAAGTAGATCTTCAGGCTATGGACCGAGCACATAGAATTGGGCAGACTAAGACAGTCA
GAGTGTTCCGCTTTATAACTGATAACACTGTAGAAGAAAGAATAGTAGAACGTGCTGAGA
TGAAACTCAGACTGGATTCAATAGTCATTCAACAAGGGAGGCTTGTGGATCAGTAACGCG
ACTTAATTAACTCGTTTAAACGGTCTCCAGCTTGGCTGTTTTGGCGG

Protein sequence predicted based on nucleotide sequence

MKPGRPRIKKDEKQNLLSVGDYRHRRTEQEEDEELLTESSKATNVCTRFEDSPSYVKWGK
LRDYQVRGLNWLISLYENGINGILADEMGLGKTLQTISLLGYMKHYRNIPGPHMVLVPKS
TLHNWMSEFKRWVPTLRSVCLIGDKEQRAAFVRDVLLPGEWDVCVTSYEMLIKEKSVFKK
FNWRYLVIDEAHRIKNEKSKLSEIVREFKTTNRLLLTGTPLQNNLHELWSLLNFLLPDVF
NSADDFDSWFDTNNCLGDQKLVERLHMVLRPFLLRRIKADVEKSLPPKKEVKIYVGLSKM
QREWYTRILMKDIDILNSAGKMDKMRLLNILMQLRKCCNHPYLFDGAEPGPPYTTDMHLV
TNSGKMVVLDKLLPKLKEQGSRVLIFSQMTRVLDILEDYCMWRNYEYCRLDGQTPHDERQ
DSINAYNEPNSTKFVFMLSTRAGGLGINLATADVVILYDSDWNPQVDLQAMDRAHRIGQT
KTVRVFRFITDNTVEERIVERAEMKLRLDSIVIQQGRLVDQ*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: kanamycin

Bacterial Selection Condition: 50ug/mL kanamycin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.

Protein Expression Results:

ProteinExpressed: Not_Applicable

ProteinPurified: Not_Applicable

SolubleProtein: Not_Applicable

Find experimental protocols in 421473

Recommended expression in:

Not Applicable

Vector Name:

pSpeedET

Synonyms:

pSpeed-ET

Sequencing Primer:

Forward: pBAD forward

Reverse: alt_bad_rev

Description:

Bacterial expression vector, arabinose or T7 induced expression, adds N-terminal MGSDKIHHHHHHENLYFQG tag; kanamycin resistance; PIPE cloning.

Comments:

If this vector contains an insert, the ccdB death cassette will not be present.

Size (bp):

5464

Parent Vector:

None

Properties:

PIPE cloning, bacterial expression, in vitro transcription, inducible expression, with tag/fusion/marker

Author Name:

Joint Center for Structural Genomics

Publications:

PMID: 18004753

Title: Combining the polymerase incomplete primer extension method for cloning and mutagenesis with microscreening to accelerate structural genomics efforts.

Vector Map:

Vector Sequence:

Sequence:

AAGAAACCAATTGTCCATATTGCATCAGACATTGCCGTCACTGCGTCTTTTACTGGCTCTTCTCGCTAACCAAACCGGTAACCCCGCTTATTAAAAGCATTCTGTAACAAAGCGGGACCAAAGCCATGACAAAAACGCGTAACAAAAGTGTCTATAATCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGCATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGTTTCTCCATACCCGTAATACGACTCACTATAGGGTTTTGTTTAACTTTAAGAAGGAGATATACATACCCATGGGATCTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGCGCCGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATAATGTGTGGATTTTGAGTTAGGATCCGGCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTCCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTTTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCGTAAACGCGTGGATCCGGCTTACTAAAAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGAATATATACTGATATGTATACCCGAAGTATGTCAAAAAGAGGTGTGCTATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCAATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAAATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACACGCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGGTGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTGGCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAACTTAATTAACTCTAGATAACTGAGAAGGAGATATACATACCCGGGTAAGTTTAAACGGTCTCCAGCTTGGCTGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTTTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGCCATATTCAACGGGAAACGTCTTGCTCTAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGATTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCTCTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGGAAAACAGCATTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGTCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAACTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCAGATCAATTCGCGCGCGAAGGCGAAGCGGCATGCATAATGTGCCTGTCAAATGGACGAAGCAGGGATTCTGCAAACCCTATGCTACTCCGTCAAGCCGTCAATTGTCTGATTCGTTACCAATTATGACAACTTGACGGCTACATCATTCACTTTTTCTTCACAACCGGCACGGAACTCGCTCGGGCTGGCCCCGGTGCATTTTTTAAATACCCGCGAGAAATAGAGTTGATCGTCAAAACCAACATTGCGACCGACGGTGGCGATAGGCATCCGGGTGGTGCTCAAAAGCAGCTTCGCCTGGCTGATACGTTGGTCCTCGCGCCAGCTTAAGACGCTAATCCCTAACTGCTGGCGGAAAAGATGTGACAGACGCGACGGCGACAAGCAAACATGCTGTGCGACGCTGGCGATATCAAAATTGCTGTCTGCCAGGTGATCGCTGATGTACTGACAAGCCTCGCGTACCCGATTATCCATCGGTGGATGGAGCGACTCGTTAATCGCTTCCATGCGCCGCAGTAACAATTGCTCAAGCAGATTTATCGCCAGCAGCTCCGAATAGCGCCCTTCCCCTTGCCCGGCGTTAATGATTTGCCCAAACAGGTCGCTGAAATGCGGCTGGTGCGCTTCATCCGGGCGAAAGAACCCCGTATTGGCAAATATTGACGGCCAGTTAAGCCATTCATGCCAGTAGGCGCGCGGACGAAAGTAAACCCACTGGTGATACCATTCGCGAGCCTCCGGATGACGACCGTAGTGATGAATCTCTCCTGGCGGGAACAGCAAAATATCACCCGGTCGGCAAACAAATTCTCGTCCCTGATTTTTCACCACCCCCTGACCGCGAATGGTGAGATTGAGAATATAACCTTTCATTCCCAGCGGTCGGTCGATAAAAAAATCGAGATAACCGTTGGCCTCAATCGGCGTTAAACCCGCCACCAGATGGGCATTAAACGAGTATCCCGGCAGCAGGGGATCATTTTGCGCTTCAGCCATACTTTTCATACTCCCGCCATTCAGAG

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

ColE1-type origin of replication

3306

3988

inducible trxn activator protein gene

araC

araC coding sequence

4563

5438

negative selection marker

ccdB

ccdB death cassette gene (lost in with-insert form)

1596

1805

promoter

T7 promoter

290

310

promoter

araC

araC promoter

256

285

protease cleavage site

TEV

TEV protease cleavage site

382

402

selectable marker

CmR

chloramphenicol resistance gene (CmR) (lost in with-insert form)

502

1158

selectable marker

KanR

kanamycin resistance gene

2396

3208

tag

His

N-terminal 6xHis tag

364

381

trxn regulatory element

arabinose O1 operator

161

182

trxn regulatory element

arabinose O2 operator

trxn termination sequence

rrnB

rrnB T2 transcription terminator

2258

2284

Gene

Gene Symbol :

SMARCA5

Symbol Nomenclature :

SMARCA5

SYNONYM :

ISWI; SNF2H; WCRF135; hISWI; hSNF2H

Designation :

SWI/SNF-related matrix-associated actin-dependent regulator of chromatin A5|SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5|sucrose nonfermenting protein 2 homolog|sucrose nonfermenting-like 5

Full Nomenclature :

SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 5

GENEID :

8467

GI :

18606276

GenBank Accession :

BC023144

HGNC :

11101

MIM :

603375

Vega :

OTTHUMG00000161474

Target GenBank:

BC023144

Reference Sequence Alignment

  

HsCD00545919      ------------------------------------------------------------
NM_003601.3       ATGTCGTCCGCGGCCGAGCCTCCGCCACCCCCGCCTCCCGAGAGCGCGCCTTCCAAGCCC
                                                                              

HsCD00545919      ------------------------------------------------------------
NM_003601.3       GCAGCCTCGATCGCCAGCGGCGGGAGCAACAGCAGCAACAAAGGCGGCCCCGAAGGCGTC
                                                                              

HsCD00545919      ------------------------------------------------------------
NM_003601.3       GCGGCGCAGGCGGTTGCGTCTGCGGCCAGCGCTGGTCCCGCAGACGCCGAGATGGAGGAA
                                                                              

HsCD00545919      ------------------------------------------------------------
NM_003601.3       ATATTTGATGATGCGTCACCTGGAAAGCAAAAGGAAATCCAAGAACCAGATCCTACCTAT
                                                                              

HsCD00545919      ------------------------------------------------------------
NM_003601.3       GAAGAAAAAATGCAAACTGACCGGGCAAATAGATTCGAGTATTTATTAAAGCAGACAGAA
                                                                              

HsCD00545919      -------CTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGCATG
NM_003601.3       CTTTTTGCACATTTCATTCAACCTGCTGCTCAGAAGACTCCAACTTCACCTTTGAAGATG
                         *: **::.*****:*.* .* .*** .* ..:....  *..    :*.. ***

HsCD00545919      AAACCAGGGCGCCCACGAATAAAAAAAGATGAGAAGCAGAACTTACTATCCGTTGGCGAT
NM_003601.3       AAACCAGGGCGCCCACGAATAAAAAAAGATGAGAAGCAGAACTTACTATCCGTTGGCGAT
                  ************************************************************

HsCD00545919      TACCGACACCGTAGAACAGAGCAAGAGGAGGATGAAGAGCTATTAACAGAAAGCTCCAAA
NM_003601.3       TACCGACACCGTAGAACAGAGCAAGAGGAGGATGAAGAGCTATTAACAGAAAGCTCCAAA
                  ************************************************************

HsCD00545919      GCAACCAATGTTTGCACTCGATTTGAAGACTCTCCATCGTATGTAAAATGGGGTAAACTG
NM_003601.3       GCAACCAATGTTTGCACTCGATTTGAAGACTCTCCATCGTATGTAAAATGGGGTAAACTG
                  ************************************************************

HsCD00545919      AGAGATTATCAGGTCCGAGGATTAAACTGGCTCATTTCTTTGTATGAGAATGGCATCAAT
NM_003601.3       AGAGATTATCAGGTCCGAGGATTAAACTGGCTCATTTCTTTGTATGAGAATGGCATCAAT
                  ************************************************************

HsCD00545919      GGTATCCTTGCAGATGAAATGGGCCTAGGAAAGACTCTTCAAACAATTTCTCTTCTTGGG
NM_003601.3       GGTATCCTTGCAGATGAAATGGGCCTAGGAAAGACTCTTCAAACAATTTCTCTTCTTGGG
                  ************************************************************

HsCD00545919      TACATGAAACATTATAGAAACATTCCTGGGCCTCATATGGTTTTGGTTCCTAAGTCTACA
NM_003601.3       TACATGAAACATTATAGAAACATTCCTGGGCCTCATATGGTTTTGGTTCCTAAGTCTACA
                  ************************************************************

HsCD00545919      TTACACAACTGGATGAGTGAATTCAAGAGATGGGTACCAACACTTAGATCTGTTTGTTTG
NM_003601.3       TTACACAACTGGATGAGTGAATTCAAGAGATGGGTACCAACACTTAGATCTGTTTGTTTG
                  ************************************************************

HsCD00545919      ATAGGAGATAAAGAACAAAGAGCTGCTTTTGTCAGAGACGTTTTATTACCGGGAGAATGG
NM_003601.3       ATAGGAGATAAAGAACAAAGAGCTGCTTTTGTCAGAGACGTTTTATTACCGGGAGAATGG
                  ************************************************************

HsCD00545919      GATGTATGTGTAACATCTTATGAAATGCTTATTAAAGAGAAGTCTGTGTTCAAAAAATTT
NM_003601.3       GATGTATGTGTAACATCTTATGAAATGCTTATTAAAGAGAAGTCTGTGTTCAAAAAATTT
                  ************************************************************

HsCD00545919      AATTGGAGATACTTAGTAATAGATGAAGCTCACAGGATCAAAAATGAAAAATCTAAGTTG
NM_003601.3       AATTGGAGATACTTAGTAATAGATGAAGCTCACAGGATCAAAAATGAAAAATCTAAGTTG
                  ************************************************************

HsCD00545919      TCAGAAATAGTGAGGGAATTCAAGACTACAAATAGACTATTATTAACTGGAACACCTCTT
NM_003601.3       TCAGAAATAGTGAGGGAATTCAAGACTACAAATAGACTATTATTAACTGGAACACCTCTT
                  ************************************************************

HsCD00545919      CAGAACAACTTGCATGAGCTGTGGTCACTTCTTAACTTTCTGTTGCCAGATGTGTTTAAT
NM_003601.3       CAGAACAACTTGCATGAGCTGTGGTCACTTCTTAACTTTCTGTTGCCAGATGTGTTTAAT
                  ************************************************************

HsCD00545919      TCAGCAGATGACTTTGATTCCTGGTTTGATACAAACAACTGCCTTGGGGATCAAAAACTA
NM_003601.3       TCAGCAGATGACTTTGATTCCTGGTTTGATACAAACAACTGCCTTGGGGATCAAAAACTA
                  ************************************************************

HsCD00545919      GTTGAGAGGCTTCATATGGTTTTGCGTCCATTCCTCCTTCGTCGAATTAAGGCTGATGTT
NM_003601.3       GTTGAGAGGCTTCATATGGTTTTGCGTCCATTCCTCCTTCGTCGAATTAAGGCTGATGTT
                  ************************************************************

HsCD00545919      GAAAAGAGTTTGCCTCCAAAGAAGGAAGTAAAAATCTATGTGGGCCTCAGCAAAATGCAA
NM_003601.3       GAAAAGAGTTTGCCTCCAAAGAAGGAAGTAAAAATCTATGTGGGCCTCAGCAAAATGCAA
                  ************************************************************

HsCD00545919      AGGGAATGGTATACTCGGATATTAATGAAGGATATAGATATACTCAACTCAGCAGGCAAG
NM_003601.3       AGGGAATGGTATACTCGGATATTAATGAAGGATATAGATATACTCAACTCAGCAGGCAAG
                  ************************************************************

HsCD00545919      ATGGACAAAATGAGGTTATTGAACATCCTAATGCAGTTGAGAAAATGTTGTAATCATCCA
NM_003601.3       ATGGACAAAATGAGGTTATTGAACATCCTAATGCAGTTGAGAAAATGTTGTAATCATCCA
                  ************************************************************

HsCD00545919      TATCTCTTTGATGGAGCAGAACCTGGTCCACCTTATACAACAGATATGCATCTAGTAACC
NM_003601.3       TATCTCTTTGATGGAGCAGAACCTGGTCCACCTTATACAACAGATATGCATCTAGTAACC
                  ************************************************************

HsCD00545919      AACAGTGGCAAAATGGTGGTTTTAGACAAGCTGCTCCCTAAGTTAAAAGAACAAGGTTCA
NM_003601.3       AACAGTGGCAAAATGGTGGTTTTAGACAAGCTGCTCCCTAAGTTAAAAGAACAAGGTTCA
                  ************************************************************

HsCD00545919      CGAGTACTAATCTTCAGTCAAATGACAAGGGTATTGGACATTTTGGAAGATTATTGCATG
NM_003601.3       CGAGTACTAATCTTCAGTCAAATGACAAGGGTATTGGACATTTTGGAAGATTATTGCATG
                  ************************************************************

HsCD00545919      TGGAGAAATTATGAGTACTGCAGGTTGGATGGTCAGACACCCCATGATGAGAGACAAGAC
NM_003601.3       TGGAGAAATTATGAGTACTGCAGGTTGGATGGTCAGACACCCCATGATGAGAGACAAGAC
                  ************************************************************

HsCD00545919      TCCATCAATGCATACAATGAACCAAACAGCACAAAGTTTGTTTTCATGTTAAGCACGCGT
NM_003601.3       TCCATCAATGCATACAATGAACCAAACAGCACAAAGTTTGTTTTCATGTTAAGCACGCGT
                  ************************************************************

HsCD00545919      GCTGGTGGTCTTGGCATCAATCTTGCGACTGCTGATGTAGTAATTTTGTATGATTCTGAT
NM_003601.3       GCTGGTGGTCTTGGCATCAATCTTGCGACTGCTGATGTAGTAATTTTGTATGATTCTGAT
                  ************************************************************

HsCD00545919      TGGAATCCCCAAGTAGATCTTCAGGCTATGGACCGAGCACATAGAATTGGGCAGACTAAG
NM_003601.3       TGGAATCCCCAAGTAGATCTTCAGGCTATGGACCGAGCACATAGAATTGGGCAGACTAAG
                  ************************************************************

HsCD00545919      ACAGTCAGAGTGTTCCGCTTTATAACTGATAACACTGTAGAAGAAAGAATAGTAGAACGT
NM_003601.3       ACAGTCAGAGTGTTCCGCTTTATAACTGATAACACTGTAGAAGAAAGAATAGTAGAACGT
                  ************************************************************

HsCD00545919      GCTGAGATGAAACTCAGACTGGATTCAATAGTCATTCAACAAGGGAGGCTTGTGGATCAG
NM_003601.3       GCTGAGATGAAACTCAGACTGGATTCAATAGTCATTCAACAAGGGAGGCTTGTGGATCAG
                  ************************************************************

HsCD00545919      TAACGCGACTTAATTAACTCGTTTAAACGGTCTCCAGCTTGGCTGTTTTGGCGG------
NM_003601.3       AATCTGAACAAAATTGGGAAAGATGAAATGCTTCAAATGATTAGACATGGAGCAACACAT
                  :*:*  .**::****.. :.. :*.**. *  **.*.  :  . . :* *.  .      

HsCD00545919      ------------------------------------------------------------
NM_003601.3       GTGTTTGCTTCAAAGGAAAGTGAGATCACTGATGAAGATATCGATGGTATTTTGGAAAGA
                                                                              

HsCD00545919      ------------------------------------------------------------
NM_003601.3       GGTGCAAAGAAGACTGCAGAGATGAATGAAAAGCTCTCCAAGATGGGCGAAAGTTCACTT
                                                                              

HsCD00545919      ------------------------------------------------------------
NM_003601.3       AGAAACTTTACAATGGATACAGAGTCAAGTGTTTATAACTTCGAAGGAGAAGACTATAGA
                                                                              

HsCD00545919      ------------------------------------------------------------
NM_003601.3       GAAAAACAAAAGATTGCATTCACAGAGTGGATTGAACCACCTAAACGAGAAAGAAAAGCC
                                                                              

HsCD00545919      ------------------------------------------------------------
NM_003601.3       AACTATGCCGTTGATGCATATTTCAGGGAAGCTCTTCGTGTTAGTGAACCTAAAGCACCC
                                                                              

HsCD00545919      ------------------------------------------------------------
NM_003601.3       AAGGCTCCTCGACCTCCAAAACAACCCAATGTTCAGGATTTCCAGTTCTTTCCTCCACGT
                                                                              

HsCD00545919      ------------------------------------------------------------
NM_003601.3       TTATTTGAATTACTGGAAAAAGAAATTCTGTTTTACAGAAAAACTATTGGGTACAAGGTA
                                                                              

HsCD00545919      ------------------------------------------------------------
NM_003601.3       CCTCGAAATCCTGAGCTGCCTAACGCAGCACAGGCACAAAAAGAAGAACAGCTTAAAATT
                                                                              

HsCD00545919      ------------------------------------------------------------
NM_003601.3       GATGAAGCTGAATCCCTTAATGATGAAGAGTTAGAGGAAAAAGAGAAGCTTCTAACACAG
                                                                              

HsCD00545919      ------------------------------------------------------------
NM_003601.3       GGATTTACCAATTGGAATAAGAGAGATTTTAACCAGTTTATCAAAGCTAATGAGAAGTGG
                                                                              

HsCD00545919      ------------------------------------------------------------
NM_003601.3       GGTCGTGATGATATTGAAAATATAGCAAGAGAAGTAGAAGGCAAAACTCCAGAAGAAGTC
                                                                              

HsCD00545919      ------------------------------------------------------------
NM_003601.3       ATTGAATATTCAGCTGTGTTTTGGGAAAGGTGCAACGAGCTCCAGGACATAGAGAAGATT
                                                                              

HsCD00545919      ------------------------------------------------------------
NM_003601.3       ATGGCTCAGATTGAAAGGGGAGAGGCGAGAATTCAAAGAAGAATAAGCATCAAGAAAGCA
                                                                              

HsCD00545919      ------------------------------------------------------------
NM_003601.3       CTTGACACAAAGATTGGACGGTACAAAGCACCTTTTCATCAGCTGAGAATATCATATGGT
                                                                              

HsCD00545919      ------------------------------------------------------------
NM_003601.3       ACTAACAAAGGAAAAAACTATACTGAAGAAGAAGATCGTTTTCTGATTTGTATGCTTCAC
                                                                              

HsCD00545919      ------------------------------------------------------------
NM_003601.3       AAACTTGGATTTGACAAAGAAAATGTTTATGATGAATTGCGACAGTGTATTCGCAACTCT
                                                                              

HsCD00545919      ------------------------------------------------------------
NM_003601.3       CCTCAGTTCAGATTTGACTGGTTTCTTAAGTCCAGAACTGCAATGGAGCTCCAGAGGAGA
                                                                              

HsCD00545919      ------------------------------------------------------------
NM_003601.3       TGTAATACCTTAATTACTTTGATTGAAAGAGAAAACATGGAACTAGAAGAAAAGGAGAAG
                                                                              

HsCD00545919      ------------------------------------------------------------
NM_003601.3       GCAGAGAAAAAGAAACGAGGACCAAAGCCTTCAACACAGAAACGTAAAATGGATGGCGCA
                                                                              

HsCD00545919      ---------------------------------------
NM_003601.3       CCTGATGGTCGAGGAAGAAAAAAGAAGCTGAAACTATGA

Pathways

Panther :

Wnt signaling pathway

Reactome :

Cell Cycle

Reactome :

Chromosome Maintenance

Reactome :

Deposition of new CENPA-containing nucleosomes at the centromere

Reactome :

Epigenetic regulation of gene expression

Reactome :

Gene Expression

Reactome :

Negative epigenetic regulation of rRNA expression

Reactome :

NoRC negatively regulates rRNA expression

Reactome :

Nucleosome assembly

Protein

SMART domain :

DEXDc : DEAD-like helicases superfamily

SMART domain :

HELICc : helicase superfamily c-terminal domain

Structural Annotation Wiki :

421473

HPRD :

04538

Clone

Cloning Information :

622461

Original Clone ID :

85582

Experimental

TargetTrack - Experimental data :

421473

Organism

Species Specific ID:

8467

Genome

Chromosome :

Map Location :

4q31.1-q31.2

Ensembl :

ENSG00000153147

Reagents

Labome :

SMARCA5-antibody