DNASU Plasmid | GFI1B (Homo sapiens) in pSpeedET (bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: GFI1B

Gene Name: growth factor independent 1B transcription repressor

Original Clone ID:

99259

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pSpeedET

Format:

CLOSED

Source:

Joint Center for Structural Genomics

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

PSI:Biology
Joint Center for Structural Genomics

HsCD00547400

Price:

AVAILABLE

No restriction
Special MTA: PSI

Insert Sequence
Protein Sequence

Insert sequence: 993nts

Open reading frame: 51 to 1043

Reference Sequence Alignment

CTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGCATGCCACGCT
CCTTCCTGGTGAAGAGCAAGAAGGCTCACACCTACCACCAGCCCCGTGTGCAGGAAGATG
AACCGCTCTGGCCTCCTGCCCTTACCCCGGTGCCCAGAGACCAGGCTCCAAGCAACAGCC
CTGTCCTTAGCACTCTATTCCCAAACCAGTGCCTGGACTGGACCAACCTCAAACGAGAGC
CGGAGCTGGAGCAGGACCAGAACTTGGCCAGGATGGCCCCGGCACCAGAGGGCCCCATTG
TGCTGTCCCGACCCCAGGATGGGGACTCTCCACTGTCCGACTCACCCCCATTCTACAAGC
CTAGCTTCTCCTGGGACACCTTGGCCACAACCTATGGCCACAGCTACCGGCAGGCCCCCT
CCACCATGCAGTCAGCCTTCCTGGAGCACTCCGTCAGCCTGTACGGCAGTCCTCTTGTGC
CCAGCACTGAGCCCGCCTTGGACTTCAGCCTCCGCTACTCCCCAGGCATGGATGCGTACC
ACTGTGTGAAGTGCAACAAGGTCTTCTCCACCCCTCACGGGCTCGAAGTGCATGTGCGAC
GCTCCCATAGTGGGACCCGGCCCTTCGCCTGTGACATCTGCGGCAAAACCTTCGGCCACG
CTGTGAGCCTGGAGCAGCACACGCACGTCCACTCCCAGGAGCGCAGCTTCGAGTGCCGCA
TGTGCGGCAAGGCCTTCAAGCGCTCGTCCACGCTGTCCACCCACCTGCTCATCCACTCAG
ACACGCGGCCCTACCCCTGCCAGTTCTGCGGCAAGCGTTTCCACCAGAAGTCCGACATGA
AGAAGCACACCTACATCCACACAGGTGAGAAGCCGCACAAGTGCCAGGTGTGCGGAAAGG
CCTTCAGCCAGAGCTCCAACCTCATCACCCACAGCCGCAAGCACACAGGCTTCAAGCCCT
TCAGCTGTGAGCTGTGCACCAAAGGCTTCCAGCGCAAGGTGGACCTGCGGCGGCACCGCG
AGAGCCAGCACAATCTCAAGTAACGCGACTTAATTAACTCGTTTAAACGGTCTCCAGCTT
GGCTGTTTTGGCGG

Protein sequence predicted based on nucleotide sequence

MPRSFLVKSKKAHTYHQPRVQEDEPLWPPALTPVPRDQAPSNSPVLSTLFPNQCLDWTNL
KREPELEQDQNLARMAPAPEGPIVLSRPQDGDSPLSDSPPFYKPSFSWDTLATTYGHSYR
QAPSTMQSAFLEHSVSLYGSPLVPSTEPALDFSLRYSPGMDAYHCVKCNKVFSTPHGLEV
HVRRSHSGTRPFACDICGKTFGHAVSLEQHTHVHSQERSFECRMCGKAFKRSSTLSTHLL
IHSDTRPYPCQFCGKRFHQKSDMKKHTYIHTGEKPHKCQVCGKAFSQSSNLITHSRKHTG
FKPFSCELCTKGFQRKVDLRRHRESQHNLK*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: kanamycin

Bacterial Selection Condition: 50ug/mL kanamycin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.

Protein Expression Results:

ProteinExpressed: Not_Applicable

ProteinPurified: Not_Applicable

SolubleProtein: Not_Applicable

Find experimental protocols in 422737

Recommended expression in:

Not Applicable

Vector Name:

pSpeedET

Synonyms:

pSpeed-ET

Sequencing Primer:

Forward: pBAD forward

Reverse: alt_bad_rev

Description:

Bacterial expression vector, arabinose or T7 induced expression, adds N-terminal MGSDKIHHHHHHENLYFQG tag; kanamycin resistance; PIPE cloning.

Comments:

If this vector contains an insert, the ccdB death cassette will not be present.

Size (bp):

5464

Parent Vector:

None

Properties:

PIPE cloning, bacterial expression, in vitro transcription, inducible expression, with tag/fusion/marker

Author Name:

Joint Center for Structural Genomics

Publications:

PMID: 18004753

Title: Combining the polymerase incomplete primer extension method for cloning and mutagenesis with microscreening to accelerate structural genomics efforts.

Vector Map:

Vector Sequence:

Sequence:

AAGAAACCAATTGTCCATATTGCATCAGACATTGCCGTCACTGCGTCTTTTACTGGCTCTTCTCGCTAACCAAACCGGTAACCCCGCTTATTAAAAGCATTCTGTAACAAAGCGGGACCAAAGCCATGACAAAAACGCGTAACAAAAGTGTCTATAATCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGCATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGTTTCTCCATACCCGTAATACGACTCACTATAGGGTTTTGTTTAACTTTAAGAAGGAGATATACATACCCATGGGATCTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGCGCCGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATAATGTGTGGATTTTGAGTTAGGATCCGGCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTCCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTTTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCGTAAACGCGTGGATCCGGCTTACTAAAAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGAATATATACTGATATGTATACCCGAAGTATGTCAAAAAGAGGTGTGCTATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCAATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAAATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACACGCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGGTGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTGGCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAACTTAATTAACTCTAGATAACTGAGAAGGAGATATACATACCCGGGTAAGTTTAAACGGTCTCCAGCTTGGCTGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTTTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGCCATATTCAACGGGAAACGTCTTGCTCTAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGATTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCTCTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGGAAAACAGCATTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGTCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAACTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCAGATCAATTCGCGCGCGAAGGCGAAGCGGCATGCATAATGTGCCTGTCAAATGGACGAAGCAGGGATTCTGCAAACCCTATGCTACTCCGTCAAGCCGTCAATTGTCTGATTCGTTACCAATTATGACAACTTGACGGCTACATCATTCACTTTTTCTTCACAACCGGCACGGAACTCGCTCGGGCTGGCCCCGGTGCATTTTTTAAATACCCGCGAGAAATAGAGTTGATCGTCAAAACCAACATTGCGACCGACGGTGGCGATAGGCATCCGGGTGGTGCTCAAAAGCAGCTTCGCCTGGCTGATACGTTGGTCCTCGCGCCAGCTTAAGACGCTAATCCCTAACTGCTGGCGGAAAAGATGTGACAGACGCGACGGCGACAAGCAAACATGCTGTGCGACGCTGGCGATATCAAAATTGCTGTCTGCCAGGTGATCGCTGATGTACTGACAAGCCTCGCGTACCCGATTATCCATCGGTGGATGGAGCGACTCGTTAATCGCTTCCATGCGCCGCAGTAACAATTGCTCAAGCAGATTTATCGCCAGCAGCTCCGAATAGCGCCCTTCCCCTTGCCCGGCGTTAATGATTTGCCCAAACAGGTCGCTGAAATGCGGCTGGTGCGCTTCATCCGGGCGAAAGAACCCCGTATTGGCAAATATTGACGGCCAGTTAAGCCATTCATGCCAGTAGGCGCGCGGACGAAAGTAAACCCACTGGTGATACCATTCGCGAGCCTCCGGATGACGACCGTAGTGATGAATCTCTCCTGGCGGGAACAGCAAAATATCACCCGGTCGGCAAACAAATTCTCGTCCCTGATTTTTCACCACCCCCTGACCGCGAATGGTGAGATTGAGAATATAACCTTTCATTCCCAGCGGTCGGTCGATAAAAAAATCGAGATAACCGTTGGCCTCAATCGGCGTTAAACCCGCCACCAGATGGGCATTAAACGAGTATCCCGGCAGCAGGGGATCATTTTGCGCTTCAGCCATACTTTTCATACTCCCGCCATTCAGAG

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

ColE1-type origin of replication

3306

3988

inducible trxn activator protein gene

araC

araC coding sequence

4563

5438

negative selection marker

ccdB

ccdB death cassette gene (lost in with-insert form)

1596

1805

promoter

T7 promoter

290

310

promoter

araC

araC promoter

256

285

protease cleavage site

TEV

TEV protease cleavage site

382

402

selectable marker

CmR

chloramphenicol resistance gene (CmR) (lost in with-insert form)

502

1158

selectable marker

KanR

kanamycin resistance gene

2396

3208

tag

His

N-terminal 6xHis tag

364

381

trxn regulatory element

arabinose O1 operator

161

182

trxn regulatory element

arabinose O2 operator

trxn termination sequence

rrnB

rrnB T2 transcription terminator

2258

2284

Gene

Gene Symbol :

GFI1B

Symbol Nomenclature :

GFI1B

SYNONYM :

BDPLT17

Designation :

growth factor independent 1B (potential regulator of CDKN1A, translocated in CML)|growth factor independent protein 1B|potential regulator of CDKN1A translocated in CML|zinc finger protein Gfi-1b

Full Nomenclature :

growth factor independent 1B transcription repressor

GENEID :

8328

Locus Tag :

RP11-415H23.1

GI :

55249536

GenBank Accession :

BC043371

HGNC :

4238

MIM :

604383

Vega :

OTTHUMG00000020848

Target GenBank:

BC043371

Reference Sequence Alignment

  

HsCD00547400      CTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGCATGCCACGCT
NM_004188.4       --------------------------------------------------ATGCCACGCT
                                                                    **********

HsCD00547400      CCTTCCTGGTGAAGAGCAAGAAGGCTCACACCTACCACCAGCCCCGTGTGCAGGAAGATG
NM_004188.4       CCTTCCTGGTGAAGAGCAAGAAGGCTCACACCTACCACCAGCCCCGTGTGCAGGAAGATG
                  ************************************************************

HsCD00547400      AACCGCTCTGGCCTCCTGCCCTTACCCCGGTGCCCAGAGACCAGGCTCCAAGCAACAGCC
NM_004188.4       AACCGCTCTGGCCTCCTGCCCTTACCCCGGTGCCCAGAGACCAGGCTCCAAGCAACAGCC
                  ************************************************************

HsCD00547400      CTGTCCTTAGCACTCTATTCCCAAACCAGTGCCTGGACTGGACCAACCTCAAACGAGAGC
NM_004188.4       CTGTCCTTAGCACTCTATTCCCAAACCAGTGCCTGGACTGGACCAACCTCAAACGAGAGC
                  ************************************************************

HsCD00547400      CGGAGCTGGAGCAGGACCAGAACTTGGCCAGGATGGCCCCGGCACCAGAGGGCCCCATTG
NM_004188.4       CGGAGCTGGAGCAGGACCAGAACTTGGCCAGGATGGCCCCGGCACCAGAGGGCCCCATTG
                  ************************************************************

HsCD00547400      TGCTGTCCCGACCCCAGGATGGGGACTCTCCACTGTCCGACTCACCCCCATTCTACAAGC
NM_004188.4       TGCTGTCCCGACCCCAGGATGGGGACTCTCCACTGTCCGACTCACCCCCATTCTACAAGC
                  ************************************************************

HsCD00547400      CTAGCTTCTCCTGGGACACCTTGGCCACAACCTATGGCCACAGCTACCGGCAGGCCCCCT
NM_004188.4       CTAGCTTCTCCTGGGACACCTTGGCCACAACCTATGGCCACAGCTACCGGCAGGCCCCCT
                  ************************************************************

HsCD00547400      CCACCATGCAGTCAGCCTTCCTGGAGCACTCCGTCAGCCTGTACGGCAGTCCTCTTGTGC
NM_004188.4       CCACCATGCAGTCAGCCTTCCTGGAGCACTCCGTCAGCCTGTACGGCAGTCCTCTTGTGC
                  ************************************************************

HsCD00547400      CCAGCACTGAGCCCGCCTTGGACTTCAGCCTCCGCTACTCCCCAGGCATGGATGCGTACC
NM_004188.4       CCAGCACTGAGCCCGCCTTGGACTTCAGCCTCCGCTACTCCCCAGGCATGGATGCGTACC
                  ************************************************************

HsCD00547400      ACTGTGTGAAGTGCAACAAGGTCTTCTCCACCCCTCACGGGCTCGAAGTGCATGTGCGAC
NM_004188.4       ACTGTGTGAAGTGCAACAAGGTCTTCTCCACCCCTCACGGGCTCGAAGTGCATGTGCGAC
                  ************************************************************

HsCD00547400      GCTCCCATAGTGGGACCCGGCCCTTCGCCTGTGACATCTGCGGCAAAACCTTCGGCCACG
NM_004188.4       GCTCCCATAGTGGGACCCGGCCCTTCGCCTGTGACATCTGCGGCAAAACCTTCGGCCACG
                  ************************************************************

HsCD00547400      CTGTGAGCCTGGAGCAGCACACGCACGTCCACTCCCAGGAGCGCAGCTTCGAGTGCCGCA
NM_004188.4       CTGTGAGCCTGGAGCAGCACACGCACGTCCACTCCCAGGAGCGCAGCTTCGAGTGCCGCA
                  ************************************************************

HsCD00547400      TGTGCGGCAAGGCCTTCAAGCGCTCGTCCACGCTGTCCACCCACCTGCTCATCCACTCAG
NM_004188.4       TGTGCGGCAAGGCCTTCAAGCGCTCGTCCACGCTGTCCACCCACCTGCTCATCCACTCAG
                  ************************************************************

HsCD00547400      ACACGCGGCCCTACCCCTGCCAGTTCTGCGGCAAGCGTTTCCACCAGAAGTCCGACATGA
NM_004188.4       ACACGCGGCCCTACCCCTGCCAGTTCTGCGGCAAGCGTTTCCACCAGAAGTCCGACATGA
                  ************************************************************

HsCD00547400      AGAAGCACACCTACATCCACACAGGTGAGAAGCCGCACAAGTGCCAGGTGTGCGGAAAGG
NM_004188.4       AGAAGCACACCTACATCCACACAGGTGAGAAGCCGCACAAGTGCCAGGTGTGCGGAAAGG
                  ************************************************************

HsCD00547400      CCTTCAGCCAGAGCTCCAACCTCATCACCCACAGCCGCAAGCACACAGGCTTCAAGCCCT
NM_004188.4       CCTTCAGCCAGAGCTCCAACCTCATCACCCACAGCCGCAAGCACACAGGCTTCAAGCCCT
                  ************************************************************

HsCD00547400      TCAGCTGTGAGCTGTGCACCAAAGGCTTCCAGCGCAAGGTGGACCTGCGGCGGCACCGCG
NM_004188.4       TCAGCTGTGAGCTGTGCACCAAAGGCTTCCAGCGCAAGGTGGACCTGCGGCGGCACCGCG
                  ************************************************************

HsCD00547400      AGAGCCAGCACAATCTCAAGTAACGCGACTTAATTAACTCGTTTAAACGGTCTCCAGCTT
NM_004188.4       AGAGCCAGCACAATCTCAAGTGA-------------------------------------
                  *********************.*                                     

HsCD00547400      GGCTGTTTTGGCGG
NM_004188.4       --------------