DNASU Plasmid | DDX47 (Homo sapiens) in pSpeedET (bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: DDX47

Gene Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 47

Original Clone ID:

102673

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pSpeedET

Format:

CLOSED

Source:

Joint Center for Structural Genomics

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

PSI:Biology
Joint Center for Structural Genomics

HsCD00547495

Price:

AVAILABLE

No restriction
Special MTA: PSI

Insert Sequence
Protein Sequence

Insert sequence: 1368nts

Open reading frame: 51 to 1418

Reference Sequence Alignment

CTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGCATGGCGGCAC
CCGAGGAACACGATTCTCCGACCGAAGCGTCCCAGCCGATTGTGGAAGAGGAGGAAACTA
AAACATTTAAAGACCTGGGTGTGACAGATGTGTTGTGTGAAGCTTGTGACCAGTTGGGAT
GGACAAAACCCACCAAGATCCAGATTGAAGCTATTCCTTTGGCCTTACAAGGTCGTGATA
TCATTGGGCTTGCAGAAACTGGCTCTGGAAAGACAGGCGCCTTTGCTTTGCCCATTCTAA
ACGCACTGCTGGAGACCCCGCAGCGTTTGTTTGCCCTAGTTCTTACCCCGACTCGGGAGC
TGGCCTTTCAGATCTCAGAGCAGTTTGAAGCCCTGGGGTCCTCTATTGGAGTGCAGAGTG
CTGTGATTGTAGGTGGAATTGATTCAATGTCTCAATCTTTGGCCCTTGCAAAAAAACCAC
ATATAATAATAGCAACTCCTGGTCGACTGATTGACCACTTGGAAAATACGAAAGGTTTCA
ACTTGAGAGCTCTCAAATACTTGGTCATGGATGAAGCCGACCGAATACTGAATATGGATT
TTGAGACAGAGGTTGACAAGATCCTCAAAGTGATTCCTCGAGATCGGAAAACATTCCTCT
TCTCTGCCACCATGACCAAGAAGGTTCAAAAACTTCAGCGAGCAGCTCTGAAGAATCCTG
TGAAATGTGCCGTTTCCTCTAAATACCAGACAGTTGAAAAATTACAGCAATATTATATTT
TTATTCCCTCTAAATTCAAGGATACCTACCTGGTTTATATTCTAAATGAATTGGCTGGAA
ACTCCTTTATGATATTCTGCAGCACCTGTAATAATACCCAGAGAACAGCTTTGCTACTGC
GAAATCTTGGCTTCACTGCCATCCCCCTCCATGGACAAATGAGTCAGAGTAAGCGCCTAG
GATCCCTTAATAAGTTTAAGGCCAAGGCCCGTTCCATTCTTCTAGCAACTGACGTTGCCA
GCCGAGGTTTGGACATACCTCATGTAGATGTGGTTGTCAACTTTGACATTCCTACCCATT
CCAAGGATTACATCCATCGAGTAGGTCGAACAGCTAGAGCTGGGCGCTCCGGAAAGGCTA
TTACTTTTGTCACACAGTATGATGTGGAACTCTTCCAGCGCATAGAACACTTAATTGGGA
AGAAACTACCAGGTTTTCCAACACAGGATGATGAGGTTATGATGCTGACAGAACGCGTCG
CTGAAGCCCAAAGGTTTGCCCGAATGGAGTTAAGGGAGCATGGAGAAAAGAAGAAACGCT
CGCGAGAGGATGCTGGAGATAATGATGACACAGAGGGTGCTATTGGTGTCAGGAACAAGG
TGGCTGGAGGAAAAATGAAGAAGCGGAAAGGCCGTTAACGCGACTTAATTAACTCGTTTA
AACGGTCTCCAGCTTGGCTGTTTTGGCGG

Protein sequence predicted based on nucleotide sequence

MAAPEEHDSPTEASQPIVEEEETKTFKDLGVTDVLCEACDQLGWTKPTKIQIEAIPLALQ
GRDIIGLAETGSGKTGAFALPILNALLETPQRLFALVLTPTRELAFQISEQFEALGSSIG
VQSAVIVGGIDSMSQSLALAKKPHIIIATPGRLIDHLENTKGFNLRALKYLVMDEADRIL
NMDFETEVDKILKVIPRDRKTFLFSATMTKKVQKLQRAALKNPVKCAVSSKYQTVEKLQQ
YYIFIPSKFKDTYLVYILNELAGNSFMIFCSTCNNTQRTALLLRNLGFTAIPLHGQMSQS
KRLGSLNKFKAKARSILLATDVASRGLDIPHVDVVVNFDIPTHSKDYIHRVGRTARAGRS
GKAITFVTQYDVELFQRIEHLIGKKLPGFPTQDDEVMMLTERVAEAQRFARMELREHGEK
KKRSREDAGDNDDTEGAIGVRNKVAGGKMKKRKGR*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: kanamycin

Bacterial Selection Condition: 50ug/mL kanamycin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.

Protein Expression Results:

ProteinExpressed: Not_Applicable

ProteinPurified: Not_Applicable

SolubleProtein: Not_Applicable

Find experimental protocols in 423984

Recommended expression in:

Not Applicable

Vector Name:

pSpeedET

Synonyms:

pSpeed-ET

Sequencing Primer:

Forward: pBAD forward

Reverse: alt_bad_rev

Description:

Bacterial expression vector, arabinose or T7 induced expression, adds N-terminal MGSDKIHHHHHHENLYFQG tag; kanamycin resistance; PIPE cloning.

Comments:

If this vector contains an insert, the ccdB death cassette will not be present.

Size (bp):

5464

Parent Vector:

None

Properties:

PIPE cloning, bacterial expression, in vitro transcription, inducible expression, with tag/fusion/marker

Author Name:

Joint Center for Structural Genomics

Publications:

PMID: 18004753

Title: Combining the polymerase incomplete primer extension method for cloning and mutagenesis with microscreening to accelerate structural genomics efforts.

Vector Map:

Vector Sequence:

Sequence:

AAGAAACCAATTGTCCATATTGCATCAGACATTGCCGTCACTGCGTCTTTTACTGGCTCTTCTCGCTAACCAAACCGGTAACCCCGCTTATTAAAAGCATTCTGTAACAAAGCGGGACCAAAGCCATGACAAAAACGCGTAACAAAAGTGTCTATAATCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGCATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGTTTCTCCATACCCGTAATACGACTCACTATAGGGTTTTGTTTAACTTTAAGAAGGAGATATACATACCCATGGGATCTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGCGCCGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATAATGTGTGGATTTTGAGTTAGGATCCGGCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTCCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTTTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCGTAAACGCGTGGATCCGGCTTACTAAAAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGAATATATACTGATATGTATACCCGAAGTATGTCAAAAAGAGGTGTGCTATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCAATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAAATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACACGCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGGTGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTGGCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAACTTAATTAACTCTAGATAACTGAGAAGGAGATATACATACCCGGGTAAGTTTAAACGGTCTCCAGCTTGGCTGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTTTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGCCATATTCAACGGGAAACGTCTTGCTCTAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGATTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCTCTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGGAAAACAGCATTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGTCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAACTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCAGATCAATTCGCGCGCGAAGGCGAAGCGGCATGCATAATGTGCCTGTCAAATGGACGAAGCAGGGATTCTGCAAACCCTATGCTACTCCGTCAAGCCGTCAATTGTCTGATTCGTTACCAATTATGACAACTTGACGGCTACATCATTCACTTTTTCTTCACAACCGGCACGGAACTCGCTCGGGCTGGCCCCGGTGCATTTTTTAAATACCCGCGAGAAATAGAGTTGATCGTCAAAACCAACATTGCGACCGACGGTGGCGATAGGCATCCGGGTGGTGCTCAAAAGCAGCTTCGCCTGGCTGATACGTTGGTCCTCGCGCCAGCTTAAGACGCTAATCCCTAACTGCTGGCGGAAAAGATGTGACAGACGCGACGGCGACAAGCAAACATGCTGTGCGACGCTGGCGATATCAAAATTGCTGTCTGCCAGGTGATCGCTGATGTACTGACAAGCCTCGCGTACCCGATTATCCATCGGTGGATGGAGCGACTCGTTAATCGCTTCCATGCGCCGCAGTAACAATTGCTCAAGCAGATTTATCGCCAGCAGCTCCGAATAGCGCCCTTCCCCTTGCCCGGCGTTAATGATTTGCCCAAACAGGTCGCTGAAATGCGGCTGGTGCGCTTCATCCGGGCGAAAGAACCCCGTATTGGCAAATATTGACGGCCAGTTAAGCCATTCATGCCAGTAGGCGCGCGGACGAAAGTAAACCCACTGGTGATACCATTCGCGAGCCTCCGGATGACGACCGTAGTGATGAATCTCTCCTGGCGGGAACAGCAAAATATCACCCGGTCGGCAAACAAATTCTCGTCCCTGATTTTTCACCACCCCCTGACCGCGAATGGTGAGATTGAGAATATAACCTTTCATTCCCAGCGGTCGGTCGATAAAAAAATCGAGATAACCGTTGGCCTCAATCGGCGTTAAACCCGCCACCAGATGGGCATTAAACGAGTATCCCGGCAGCAGGGGATCATTTTGCGCTTCAGCCATACTTTTCATACTCCCGCCATTCAGAG

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

ColE1-type origin of replication

3306

3988

inducible trxn activator protein gene

araC

araC coding sequence

4563

5438

negative selection marker

ccdB

ccdB death cassette gene (lost in with-insert form)

1596

1805

promoter

T7 promoter

290

310

promoter

araC

araC promoter

256

285

protease cleavage site

TEV

TEV protease cleavage site

382

402

selectable marker

CmR

chloramphenicol resistance gene (CmR) (lost in with-insert form)

502

1158

selectable marker

KanR

kanamycin resistance gene

2396

3208

tag

His

N-terminal 6xHis tag

364

381

trxn regulatory element

arabinose O1 operator

161

182

trxn regulatory element

arabinose O2 operator

trxn termination sequence

rrnB

rrnB T2 transcription terminator

2258

2284

Gene

Gene Symbol :

DDX47

Symbol Nomenclature :

DDX47

SYNONYM :

E4-DBP; HQ0256; MSTP162; RRP3

Designation :

DEAD box polypeptide 47|DEAD box protein 47|E4-DEAD box protein|probable ATP-dependent RNA helicase DDX47

Full Nomenclature :

DEAD (Asp-Glu-Ala-Asp) box polypeptide 47

GENEID :

51202

GI :

45786091

GenBank Accession :

BC068009

HGNC :

18682

MIM :

615428

Vega :

OTTHUMG00000168709

Target GenBank:

BC068009

Reference Sequence Alignment

  

HsCD00547495      CTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGCATGGCGGCAC
NM_016355.3       --------------------------------------------------ATGGCGGCAC
                                                                    **********

HsCD00547495      CCGAGGAACACGATTCTCCGACCGAAGCGTCCCAGCCGATTGTGGAAGAGGAGGAAACTA
NM_016355.3       CCGAGGAACACGATTCTCCGACCGAAGCGTCCCAGCCGATTGTGGAAGAGGAGGAAACTA
                  ************************************************************

HsCD00547495      AAACATTTAAAGACCTGGGTGTGACAGATGTGTTGTGTGAAGCTTGTGACCAGTTGGGAT
NM_016355.3       AAACATTTAAAGACCTGGGTGTGACAGATGTGTTGTGTGAAGCTTGTGACCAGTTGGGAT
                  ************************************************************

HsCD00547495      GGACAAAACCCACCAAGATCCAGATTGAAGCTATTCCTTTGGCCTTACAAGGTCGTGATA
NM_016355.3       GGACAAAACCCACCAAGATCCAGATTGAAGCTATTCCTTTGGCCTTACAAGGTCGTGATA
                  ************************************************************

HsCD00547495      TCATTGGGCTTGCAGAAACTGGCTCTGGAAAGACAGGCGCCTTTGCTTTGCCCATTCTAA
NM_016355.3       TCATTGGGCTTGCAGAAACTGGCTCTGGAAAGACAGGCGCCTTTGCTTTGCCCATTCTAA
                  ************************************************************

HsCD00547495      ACGCACTGCTGGAGACCCCGCAGCGTTTGTTTGCCCTAGTTCTTACCCCGACTCGGGAGC
NM_016355.3       ACGCACTGCTGGAGACCCCGCAGCGTTTGTTTGCCCTAGTTCTTACCCCGACTCGGGAGC
                  ************************************************************

HsCD00547495      TGGCCTTTCAGATCTCAGAGCAGTTTGAAGCCCTGGGGTCCTCTATTGGAGTGCAGAGTG
NM_016355.3       TGGCCTTTCAGATCTCAGAGCAGTTTGAAGCCCTGGGGTCCTCTATTGGAGTGCAGAGTG
                  ************************************************************

HsCD00547495      CTGTGATTGTAGGTGGAATTGATTCAATGTCTCAATCTTTGGCCCTTGCAAAAAAACCAC
NM_016355.3       CTGTGATTGTAGGTGGAATTGATTCAATGTCTCAATCTTTGGCCCTTGCAAAAAAACCAC
                  ************************************************************

HsCD00547495      ATATAATAATAGCAACTCCTGGTCGACTGATTGACCACTTGGAAAATACGAAAGGTTTCA
NM_016355.3       ATATAATAATAGCAACTCCTGGTCGACTGATTGACCACTTGGAAAATACGAAAGGTTTCA
                  ************************************************************

HsCD00547495      ACTTGAGAGCTCTCAAATACTTGGTCATGGATGAAGCCGACCGAATACTGAATATGGATT
NM_016355.3       ACTTGAGAGCTCTCAAATACTTGGTCATGGATGAAGCCGACCGAATACTGAATATGGATT
                  ************************************************************

HsCD00547495      TTGAGACAGAGGTTGACAAGATCCTCAAAGTGATTCCTCGAGATCGGAAAACATTCCTCT
NM_016355.3       TTGAGACAGAGGTTGACAAGATCCTCAAAGTGATTCCTCGAGATCGGAAAACATTCCTCT
                  ************************************************************

HsCD00547495      TCTCTGCCACCATGACCAAGAAGGTTCAAAAACTTCAGCGAGCAGCTCTGAAGAATCCTG
NM_016355.3       TCTCTGCCACCATGACCAAGAAGGTTCAAAAACTTCAGCGAGCAGCTCTGAAGAATCCTG
                  ************************************************************

HsCD00547495      TGAAATGTGCCGTTTCCTCTAAATACCAGACAGTTGAAAAATTACAGCAATATTATATTT
NM_016355.3       TGAAATGTGCCGTTTCCTCTAAATACCAGACAGTTGAAAAATTACAGCAATATTATATTT
                  ************************************************************

HsCD00547495      TTATTCCCTCTAAATTCAAGGATACCTACCTGGTTTATATTCTAAATGAATTGGCTGGAA
NM_016355.3       TTATTCCCTCTAAATTCAAGGATACCTACCTGGTTTATATTCTAAATGAATTGGCTGGAA
                  ************************************************************

HsCD00547495      ACTCCTTTATGATATTCTGCAGCACCTGTAATAATACCCAGAGAACAGCTTTGCTACTGC
NM_016355.3       ACTCCTTTATGATATTCTGCAGCACCTGTAATAATACCCAGAGAACAGCTTTGCTACTGC
                  ************************************************************

HsCD00547495      GAAATCTTGGCTTCACTGCCATCCCCCTCCATGGACAAATGAGTCAGAGTAAGCGCCTAG
NM_016355.3       GAAATCTTGGCTTCACTGCCATCCCCCTCCATGGACAAATGAGTCAGAGTAAGCGCCTAG
                  ************************************************************

HsCD00547495      GATCCCTTAATAAGTTTAAGGCCAAGGCCCGTTCCATTCTTCTAGCAACTGACGTTGCCA
NM_016355.3       GATCCCTTAATAAGTTTAAGGCCAAGGCCCGTTCCATTCTTCTAGCAACTGACGTTGCCA
                  ************************************************************

HsCD00547495      GCCGAGGTTTGGACATACCTCATGTAGATGTGGTTGTCAACTTTGACATTCCTACCCATT
NM_016355.3       GCCGAGGTTTGGACATACCTCATGTAGATGTGGTTGTCAACTTTGACATTCCTACCCATT
                  ************************************************************

HsCD00547495      CCAAGGATTACATCCATCGAGTAGGTCGAACAGCTAGAGCTGGGCGCTCCGGAAAGGCTA
NM_016355.3       CCAAGGATTACATCCATCGAGTAGGTCGAACAGCTAGAGCTGGGCGCTCCGGAAAGGCTA
                  ************************************************************

HsCD00547495      TTACTTTTGTCACACAGTATGATGTGGAACTCTTCCAGCGCATAGAACACTTAATTGGGA
NM_016355.3       TTACTTTTGTCACACAGTATGATGTGGAACTCTTCCAGCGCATAGAACACTTAATTGGGA
                  ************************************************************

HsCD00547495      AGAAACTACCAGGTTTTCCAACACAGGATGATGAGGTTATGATGCTGACAGAACGCGTCG
NM_016355.3       AGAAACTACCAGGTTTTCCAACACAGGATGATGAGGTTATGATGCTGACAGAACGCGTCG
                  ************************************************************

HsCD00547495      CTGAAGCCCAAAGGTTTGCCCGAATGGAGTTAAGGGAGCATGGAGAAAAGAAGAAACGCT
NM_016355.3       CTGAAGCCCAAAGGTTTGCCCGAATGGAGTTAAGGGAGCATGGAGAAAAGAAGAAACGCT
                  ************************************************************

HsCD00547495      CGCGAGAGGATGCTGGAGATAATGATGACACAGAGGGTGCTATTGGTGTCAGGAACAAGG
NM_016355.3       CGCGAGAGGATGCTGGAGATAATGATGACACAGAGGGTGCTATTGGTGTCAGGAACAAGG
                  ************************************************************

HsCD00547495      TGGCTGGAGGAAAAATGAAGAAGCGGAAAGGCCGTTAACGCGACTTAATTAACTCGTTTA
NM_016355.3       TGGCTGGAGGAAAAATGAAGAAGCGGAAAGGCCGTTAA----------------------
                  **************************************                      

HsCD00547495      AACGGTCTCCAGCTTGGCTGTTTTGGCGG
NM_016355.3       -----------------------------