Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
APC109010.103
Keyword:
None
Species:
Micromonospora echinospora
Type:
cDNA
Vector Name:
pMCSG73
Format:
FUSION
Source:
Midwest Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
Argonne National Laboratory
Robert Jedrzejczak
PSI:Biology
Midwest Center for Structural Genomics
Robert Jedrzejczak
PSI:Biology
Midwest Center for Structural Genomics
MeCD00583374
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 1500nts
Open reading frame: 12 to 1511
AATCCAATGCCATGGGTCTTCGTGACGTCAGCGTGCTGCTGCTGTACCCGCCGAACCAGA
CCGCCCCCGGCACGGTGTGCAAGCCGAACGGATCGCTCGCGTACCCGAATCTGGGCGGAG
CCCTGCGGGAGCACGGTGTGCACGTTCGCGTCTTCGACGCCTGTGTCGGCGAGGGCGCCG
ACCCGCTCGACGACATCTTCGCCAACCCCACGCCGATGCCGAGCGGTTTCCTCCGCACCG
GCGTCAGCGACGAGCGGATCCTCGAGGTGGTGAAGGACCACGACATCGTCGGCATCACCT
CGATCTTCACCGACCAGGAGACGATGGTCCTGCACTGCGCGCGGCTCATCCGGGCGGCCT
ACCCGGAGAAGCTGCTCGTCACCGGCGGGGTCAACGCGCGGTCGCGGTTGCCGCAGTTCT
TCGGGGCGGGCTTCGACGTGGTCTGCCTGTCCGAGGCCGAGCGCACGATCATCGACATCG
TCGAGGCCGTACGACGGTCGCCGCACCCGAGCTTCGCCGACATCCACGGTGTCGCCTTCC
TCGACGACGGCCGGATCCGGATGAACCCCGCGCGGGCGCAGGACGTCGTGTGGGACCTGG
ACACCCTGCCCATGCCGGCCTGGGACCTGCTGCCCAACGAACGGTACTGGACGATCGCCC
GGCCGCACTCCGGTCTCTTCGAGCCCGGCGCCGAGCTGCGGTACGCGACCATGATGACCT
CGCTCGGCTGCCCGTTCCGCTGCGCGTACTGCCACATCGCCGGCGAGCAGGAGGGGAGCC
CCAGCGGCCCGATCGGCAAGTTCCGCGTCAAGTCGGACGAGCGGGTGCTGGCGGAGATCG
AGACGCTGAAGAGCCTGGGCGTCGACAACGTGTTCATCGAGGACGACTCGCTCCTGGGCG
ACAAGCGGCGCGGCCTCCGGCTGCTCAAGAAGATCCAGGGCGCTGGCGTGACCATCTGCG
ACCTCAACGGCATCAACCTCATCCACCTGCTCAAGCGCTGGAAGCCCGACCACGAGGTCC
TGGAGGCGATGGCCGACGCGGGCTTCACGCAGATCAACATGCCCTTCGAGTCGGGCAACC
TGCGGATCATCCGCAAGTACGCGAGCAACAAGCTCAACATCGAGAAGGCCGACCTGCCCG
GGCTGATCGTCGCCATGAAGGAGTACGGCTTCCAGATCTACGGCAACTACATGATGGGTT
ACCCGGACGAGACGCTGGAGGAGATCGAGACGACCATCCAGCTCGCCAAGGAGCACGTCT
CCTACGGGCTGGACGCCGCGAACTTCTTCCTCGTCGTGCCGCTGCCCGGCGCGCCGTTGT
TCGACATGGCGATCGCGGGCGGCCACATCAGCCCCGACTTCGACCCGGACACGATGAACA
TCTACCGGGCCAACATGACCGGCACCCTGGTGCCGGCAGAGGTCCTGGAGGAGCTGCGCC
ACCGAGCCTGGGACGAGGTGAACTCGCCGACCTGGAAGAACGCCAAGAAGGCGTGGGCGG
CGACGGCCAGC
Protein sequence predicted based on nucleotide sequence
MGLRDVSVLLLYPPNQTAPGTVCKPNGSLAYPNLGGALREHGVHVRVFDACVGEGADPLD DIFANPTPMPSGFLRTGVSDERILEVVKDHDIVGITSIFTDQETMVLHCARLIRAAYPEK LLVTGGVNARSRLPQFFGAGFDVVCLSEAERTIIDIVEAVRRSPHPSFADIHGVAFLDDG RIRMNPARAQDVVWDLDTLPMPAWDLLPNERYWTIARPHSGLFEPGAELRYATMMTSLGC PFRCAYCHIAGEQEGSPSGPIGKFRVKSDERVLAEIETLKSLGVDNVFIEDDSLLGDKRR GLRLLKKIQGAGVTICDLNGINLIHLLKRWKPDHEVLEAMADAGFTQINMPFESGNLRII RKYASNKLNIEKADLPGLIVAMKEYGFQIYGNYMMGYPDETLEEIETTIQLAKEHVSYGL DAANFFLVVPLPGAPLFDMAIAGGHISPDFDPDTMNIYRANMTGTLVPAEVLEELRHRAW DEVNSPTWKNAKKAWAATAS
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
c153t,C51C;a767g,N256S
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in MCSG-APC109010
Recommended expression in:
Not Applicable
Vector Name:
pMCSG73
Synonyms:
''
Sequencing Primer:
Forward:
MCSG73F
Reverse:
T7 terminator
Description:
Bacterial expression vector with a T7 promoter and TVMV-cleavable N-terminal NusA tag and TEV-cleavable N-terminal 6xHis and StrepII tag; Contains rate argU and ileX tRNA genes; ampicillin resistance in bacteria; ligation independent cloning.
Comments:
None
Size (bp):
6227
Parent Vector:
Name:
pMCSG53
Description:
Properties:
bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name:
Midwest Center for Structural Genomics
PSI:Biology
Midwest Center for Structural Genomics
PSI:Biology
Midwest Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
pBR322 origin
pBR322 origin of replication
4220
4808
ligation independent cloning
LIC
ligation independent cloning site
138
16
promoter
T7
T7 promoter
1779
1795
protease cleavage site
TVMV
TVMV protease cleavage site
218
238
protease cleavage site
TEV
TEV protease cleavage site
155
175
repressor binding site
lac operator
lac operator
1754
1776
repressor protein gene
LacI
LacI CDS
2832
3791
selectable marker
AmpR
ampicillin resistance gene
4979
5839
tRNA
ileX
ileX tRNA gene
2378
2529
tRNA
argU
argU tRNA
1862
2218
tag
NusA
N-terminal NusA
249
1708
tag
His
N-terminal 6xHis tag
200
217
tag
strep
N-terminal StrepII tag
176
199
trxn termination sequence
T7 term
T7 terminator
26
72
