DNASU Plasmid | NDUFAF6 (H. sapiens )<br>In pET15_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: NDUFAF6

Gene Name: NADH dehydrogenase (ubiquinone) complex I, assembly factor 6

Original Clone ID:

HR6549A-69-333-15.5

Keyword:

targeted domain

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pET15_NESG

Format:

CLOSED

Source:

Northeast Structural Genomics Consortium

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

PSI:Biology
Northeast Structural Genomics Consortium

HsCD00583690

Price:

AVAILABLE

No restriction
Special MTA: PSI/NESG

Insert Sequence
Protein Sequence

Insert sequence: 801nts

Open reading frame: 51 to 851

Reference Sequence Alignment

ACTTTAGAAGGAGATATACCATGGGCCATCACCATCACCATCACAGCCATATGGATTATG
AAAGTTATTTATGCTCCCTGCTGCTCCCTGCAGAATCCCGAAGCTTTGTTTTTGCACTGA
GGGCCTTTAATGTGGAACTGGCTCAGGTTAAAGACTCAGTCTCTGAGAAAACAATTGGAC
TGATGCGAATGCAGTTTTGGAAAAAAACTGTGGAAGATATATACTGTGACAATCCACCAC
ATCAGCCTGTGGCCATTGAACTATGGAAGGCTGTTAAAAGACATAATCTGACTAAAAGAT
GGCTTATGAAAATCGTCGATGAAAGAGAAAAAAATCTGGATGACAAAGCATATCGTAATA
TCAAGGAACTGGAAAATTATGCTGAAAACACACAGAGCTCTCTTCTTTACTTAACACTAG
AAATATTGGGTATAAAGGATCTTCATGCAGATCATGCTGCAAGTCATATTGGAAAAGCAC
AAGGCATTGTCACTTGCTTGAGAGCAACACCATATCATGGGAGCAGAAGAAAGGTGTTCC
TTCCCATGGATATTTGTATGCTGCATGGTGTTTCACAAGAGGACTTTCTACGGAGGAACC
AAGATAAAAATGTGAGAGATGTAATATATGACATTGCCAGTCAAGCACACTTGCACCTAA
AGCATGCTAGGTCCTTTCACAAAACTGTTCCTGTGAAAGCATTTCCTGCTTTTCTTCAGA
CGGTTTCTCTAGAGGACTTTCTAAAGAAAATTCAGCGAGTGGATTTTGATATATTCCACC
CATCTTTACAGCAGAAGAATACATTACTTCCATTATATTTGTATATTCAGTCATGGAGAA
AAACATATTAG

Protein sequence predicted based on nucleotide sequence

MDYESYLCSLLLPAESRSFVFALRAFNVELAQVKDSVSEKTIGLMRMQFWKKTVEDIYCD
NPPHQPVAIELWKAVKRHNLTKRWLMKIVDEREKNLDDKAYRNIKELENYAENTQSSLLY
LTLEILGIKDLHADHAASHIGKAQGIVTCLRATPYHGSRRKVFLPMDICMLHGVSQEDFL
RRNQDKNVRDVIYDIASQAHLHLKHARSFHKTVPVKAFPAFLQTVSLEDFLKKIQRVDFD
IFHPSLQQKNTLLPLYLYIQSWRKTY*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

g13a,G5S;c56t,S19F

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinPurified: Not_Tested

ProteinExpressed: Protein_Confirmed

SolubleProtein: Tested_Not_Soluble

Find experimental protocols in HR6549A

Recommended expression in:

Not Applicable

Vector Name:

pET15_NESG

Synonyms:

pET15, pET15-modified, pET15Cc, pET15_NESG

Sequencing Primer:

Forward: T7

Reverse: T7 terminator

Description:

Bacterial (E. coli) expression vector with T7 lac promoter, adds N-terminal 6x His tag; amp resistance; restriction enzyme cloning.

Comments:

Modified Novagen pET vector-common MCS

Size (bp):

5723

Parent Vector:

None

Properties:

bacterial expression, in vitro transcription, multiple cloning site, with tag/fusion/marker

Author Name:

Gaetano T. Montleione
Jessica Bearden
Northeast Structural Genomics Consortium

Publications:

PMID: 15808222

Title: Robotic cloning and Protein Production Platform of the Northeast Structural Genomics Consortium

Vector Map:

Vector Sequence:

Sequence:

TTCTCATGTTTGACAGCTTATCATCGATAAGCTAGCTTTAATGCGGTAGTTTATCACAGTTAAATTGCTAACGCAGTCAGGCACCGTGTATGAAATCTAACAATGCGCTCATCGTCATCCTCGGCACCGTCACCCTGGATGCTGTAGGCATAGGCTTGGTTATGCCGGTACTGCCGGGCCTCTTGCGGGATATCCGGATATAGTTCCTCCTTTCAGCAAAAAACCCCTCAAGACCCGTTTAGAGGCCCCAAGGGGTTATGCTAGTTATTGCTCAGCGGTGGCAGCAGCCAACTCAGCTTCCTTTCGGGCTTTGTTAGCAGCCGGATCTCGAGAAGCTTTGCGTCGACAGAGCTCGCAGGATCCGCAGAATTCGCCATATGGCTGTGATGGTGATGGTGATGGCCCATGGTATATCTCCTTCTTAAAGTTAAACAAAATTATTTCTAGAGGGGAATTGTTATCCGCTCACAATTCCCCTATAGTGAGTCGTATTAATTTCGCGGGATCGAGATCTCGATCCTCTACGCCGGACGCATCGTGGCCGGCATCACCGGCGCCACAGGTGCGGTTGCTGGCGCCTATATCGCCGACATCACCGATGGGGAAGATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTGTTTCGGCGTGGGTATGGTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCTTGCATGCACCATTCCTTGCGGCGGCGGTGCTCAACGGCCTCAACCTACTACTGGGCTGCTTCCTAATGCAGGAGTCGCATAAGGGAGAGCGTCGAGATCCCGGACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAATGTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAATGCTGAATGAGGGCATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTGCGGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTTATATCCCGCCGTTAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTAAGTTAGCTCACTCATTAGGCACCGGGATCTCGACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACTATCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGCGCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCCTTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTCCCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTGTCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACCAGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGCCTCGGCGAGCACATGGAACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGTCGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACGGATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAACCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCATCTCGGGCAGCGTTGGGTCCTGGCCACGGGTGCGCATGATCGTGCTCCTGTCGTTGAGGACCCGGCTAGGCTGGCGGGGTTGCCTTACTGGTTAGCAGAATGAATCACCGATACGCGAGCGAACGTGAAGCGACTGCTGCTGCAAAACGTCTGCGACCTGAGCAACAACATGAATGGTCTTCGGTTTCCGTGTTTCGTAAAGTCTGGAAACGCGGAAGTCAGCGCCCTGCACCATTATGTTCCGGATCTGCATCGCAGGATGCTGCTGGCTACCCTGTGGAACACCTACATCTGTATTAACGAAGCGCTGGCATTGACCCTGAGTGATTTTTCTCTGGTCCCGCCGCATCCATACCGCCAGTTGTTTACCCTCACAACGTTCCAGTAACCGGGCATGTTCATCATCAGTAACCCGTATCGTGAGCATCCTCTCTCGTTTCATCGGTATCATTACCCCCATGAACAGAAATCCCCCTTACACGGAGGCATCAGTGACCAAACAGGAAAAAACCGCCCTTAACATGGCCCGCTTTATCAGAAGCCAGACATTAACGCTTCTGGAGAAACTCAACGAGCTGGACGCGGATGAACAGGCAGACATCTGTGAATCGCTTCACGACCACGCTGATGAGCTTTACCGCAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTGCAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAACACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTTCAAGAA

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (includes SphI, XbaI, EcoRI, BamHI, XhoI)

327

452

bacterial origin

ori

ColE1 origin of replication

3881

4563

gene fragment

ROP

repressor of primer ROP sequence

3281

3457

primer

T7 forward

T7 forward sequencing primer

481

496

primer

T7 reverse

T7 reverse sequencing primer

257

275

promoter

T7 promoter

478

497

repressor binding site

lacO

lacO regulatory sequence

456

478

selectable marker

AmpR

ampicillin resistance gene

4661

5230

tag

His

N-terminal 6x His tag

387

404

trxn termination sequence

T7 term

T7 termination sequence

257

275

Gene

Protein

Clone

Experimental

Organism

Genome
Reagents

Gene

Gene Symbol :

NDUFAF6

Symbol Nomenclature :

NDUFAF6

SYNONYM :

C8orf38

Designation :

UPF0551 protein C8orf38, mitochondrial|putative phytoene synthase

Full Nomenclature :

NADH dehydrogenase (ubiquinone) complex I, assembly factor 6

GENEID :

137682

GI :

124517691

GenBank Accession :

NM_152416

HGNC :

28625

MIM :

612392

Vega :

OTTHUMG00000150173

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00583690      ------------------------------------------------------------
NM_152416.3       ATGGCGGCCTCCGCGCACGGCTCTGTCTGGGGGCCGTTGCGGCTTGGCATCCCCGGCCTG
                                                                              

HsCD00583690      ------------------------------------------------------------
NM_152416.3       TGCTGCCGCCGGCCGCCTCTGGGTCTGTACGCGCGCATGCGGCGGCTGCCCGGGCCGGAG
                                                                              

HsCD00583690      -------------------------------ACTTTAGAAGGAGATATACCATGGGCCAT
NM_152416.3       GTGTCTGGGCGGAGCGTGGCTGCGGCCAGCGGACCGGGCGCCTGGGGCACTGACCACTAC
                                                 ..   .*..  :*. . ** .:  .* * 

HsCD00583690      CACCATCACCATCACAGCCATATGGATTATGAAAGTTATTTATGCTCCCTGCTGCTCCCT
NM_152416.3       TGCCTGGAGCTGCTGCGGAAACGGGATTATGAAGGTTATTTATGCTCCCTGCTGCTCCCT
                   .**:  * *: *: .* .*:. **********.**************************

HsCD00583690      GCAGAATCCCGAAGCTTTGTTTTTGCACTGAGGGCCTTTAATGTGGAACTGGCTCAGGTT
NM_152416.3       GCAGAATCCCGAAGCTCTGTTTTTGCACTGAGGGCCTTTAATGTGGAACTGGCTCAGGTT
                  **************** *******************************************

HsCD00583690      AAAGACTCAGTCTCTGAGAAAACAATTGGACTGATGCGAATGCAGTTTTGGAAAAAAACT
NM_152416.3       AAAGACTCAGTCTCTGAGAAAACAATTGGACTGATGCGAATGCAGTTTTGGAAAAAAACT
                  ************************************************************

HsCD00583690      GTGGAAGATATATACTGTGACAATCCACCACATCAGCCTGTGGCCATTGAACTATGGAAG
NM_152416.3       GTGGAAGATATATACTGTGACAATCCACCACATCAGCCTGTGGCCATTGAACTATGGAAG
                  ************************************************************

HsCD00583690      GCTGTTAAAAGACATAATCTGACTAAAAGATGGCTTATGAAAATCGTCGATGAAAGAGAA
NM_152416.3       GCTGTTAAAAGACATAATCTGACTAAAAGATGGCTTATGAAAATCGTCGATGAAAGAGAA
                  ************************************************************

HsCD00583690      AAAAATCTGGATGACAAAGCATATCGTAATATCAAGGAACTGGAAAATTATGCTGAAAAC
NM_152416.3       AAAAATCTGGATGACAAAGCATATCGTAATATCAAGGAACTGGAAAATTATGCTGAAAAC
                  ************************************************************

HsCD00583690      ACACAGAGCTCTCTTCTTTACTTAACACTAGAAATATTGGGTATAAAGGATCTTCATGCA
NM_152416.3       ACACAGAGCTCTCTTCTTTACTTAACACTAGAAATATTGGGTATAAAGGATCTTCATGCA
                  ************************************************************

HsCD00583690      GATCATGCTGCAAGTCATATTGGAAAAGCACAAGGCATTGTCACTTGCTTGAGAGCAACA
NM_152416.3       GATCATGCTGCAAGTCATATTGGAAAAGCACAAGGCATTGTCACTTGCTTGAGAGCAACA
                  ************************************************************

HsCD00583690      CCATATCATGGGAGCAGAAGAAAGGTGTTCCTTCCCATGGATATTTGTATGCTGCATGGT
NM_152416.3       CCATATCATGGGAGCAGAAGAAAGGTGTTCCTTCCCATGGATATTTGTATGCTGCATGGT
                  ************************************************************

HsCD00583690      GTTTCACAAGAGGACTTTCTACGGAGGAACCAAGATAAAAATGTGAGAGATGTAATATAT
NM_152416.3       GTTTCACAAGAGGACTTTCTACGGAGGAACCAAGATAAAAATGTGAGAGATGTAATATAT
                  ************************************************************

HsCD00583690      GACATTGCCAGTCAAGCACACTTGCACCTAAAGCATGCTAGGTCCTTTCACAAAACTGTT
NM_152416.3       GACATTGCCAGTCAAGCACACTTGCACCTAAAGCATGCTAGGTCCTTTCACAAAACTGTT
                  ************************************************************

HsCD00583690      CCTGTGAAAGCATTTCCTGCTTTTCTTCAGACGGTTTCTCTAGAGGACTTTCTAAAGAAA
NM_152416.3       CCTGTGAAAGCATTTCCTGCTTTTCTTCAGACGGTTTCTCTAGAGGACTTTCTAAAGAAA
                  ************************************************************

HsCD00583690      ATTCAGCGAGTGGATTTTGATATATTCCACCCATCTTTACAGCAGAAGAATACATTACTT
NM_152416.3       ATTCAGCGAGTGGATTTTGATATATTCCACCCATCTTTACAGCAGAAGAATACATTACTT
                  ************************************************************

HsCD00583690      CCATTATATTTGTATATTCAGTCATGGAGAAAAACATATTAG
NM_152416.3       CCATTATATTTGTATATTCAGTCATGGAGAAAAACATATTAA
                  *****************************************.

Protein

Structural Annotation Wiki :

HR6549A

UniProt :

Q330K2

HPRD :

17553

PDBe Mol* Structure Viewer

Clone

Cloning Information :

626456

Original Clone ID :

HR6549A-69-333-15.5

Experimental

TargetTrack - Experimental data :

HR6549A

Organism

Species Specific ID:

137682

Genome

Chromosome :

Map Location :

8q22.1

Ensembl :

ENSG00000156170

Reagents

Labome :

NDUFAF6-antibody

NDUFAF6 (H. sapiens )In pET15_NESG (His-tagged bacterial expression vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Reference Sequence Alignment

Protein

PDBe Mol* Structure Viewer

Clone

Experimental

Organism

Genome

Reagents

NDUFAF6 (H. sapiens )
In pET15_NESG (His-tagged bacterial expression vector)