DNASU Plasmid | SKIV2L2 (H. sapiens )<br>In pET15_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: SKIV2L2

Gene Name: superkiller viralicidic activity 2-like 2 (S. cerevisiae)

Original Clone ID:

HR9170B-140-545-15.1

Keyword:

targeted domain

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pET15_NESG

Format:

CLOSED

Source:

Northeast Structural Genomics Consortium

Description:

None

Comments:

None

Mutation / Discrepancy:

No / Yes

Publications:

None

Authors:

PSI:Biology
Northeast Structural Genomics Consortium

HsCD00583776

Price:

AVAILABLE

No restriction
Special MTA: PSI/NESG

Insert Sequence
Protein Sequence

Insert sequence: 1224nts

Open reading frame: 3 to 1226

Reference Sequence Alignment

ATATGCTTGATGCTTTTCAAAGAGAGGCCATTCAGTGTGTTGACAATAATCAGTCTGTTC
TAGTATCTGCACATACCTCAGCGGGAAAAACAGTATGCGCCGAGTATGCCATTGCATTGG
CCTTAAGGGAAAAGCAGCGTGTAATATTTACCAGCCCAATTAAGGCTCTGAGTAACCAAA
AATACCGTGAAATGTATGAAGAATTTCAAGATGTTGGTTTGATGACTGGTGATGTTACTA
TTAATCCTACGGCATCTTGTCTTGTTATGACCACAGAGATTTTGAGAAGTATGCTTTACA
GAGGTTCCGAAGTTATGAGAGAAGTTGCTTGGGTTATATTTGATGAAATTCATTATATGA
GAGATTCAGAACGTGGTGTAGTATGGGAAGAAACTATTATTTTGCTTCCTGATAACGTCC
ACTATGTCTTTCTTTCGGCTACTATTCCAAATGCCCGACAGTTTGCTGAATGGATTTGCC
ATTTACATAAACAGCCTTGTCATGTTATTTACACAGATTATCGGCCCACTCCATTGCAAC
ACTACATTTTTCCAGCAGGGGGAGATGGCCTGCATCTTGTGGTTGATGAAAATGGTGACT
TCAGAGAAGATAATTTTAATACTGCAATGCAAGTGCTTCGAGATGCAGGTGATTTGGCCA
AAGGAGACCAGAAAGGGCGGAAAGGAGGAACAAAAGGACCATCAAATGTTTTCAAAATTG
TGAAGATGATTATGGAAAGAAATTTCCAACCTGTGATTATTTTCAGTTTTAGTAAGAAAG
ATTGTGAAGCCTATGCACTTCAAATGACCAAATTAGATTTCAACACAGATGAAGAAAAAA
AGATGGTTGAAGAAGTATTCAGTAATGCAATTGATTGCTTATCCGATGAAGATAAAAAAC
TCCCTCAGGTAGAACATGTACTTCCTCTTTTGAAGAGGGGAATTGGTATTCACCATGGTG
GTTTACTTCCTATTTTGAAAGAAACTATAGAAATTCTCTTTTCTGAAGGATTGATAAAGG
CCTTATTTGCCACGGAGACCTTTGCTATGGGAATTAACATGCCAGCTAGAACTGTTTTAT
TTACAAATGCCCGCAAATTTGATGGGAAGGATTTCCGATGGATTTCTTCTGGTGAATACA
TTCAGATGTCTGGTCGTGCTGGAAGGAGAGGAATGGATGATAGAGGAATTGTAATTCTTA
TGGTAGATGAAAAGATGAGCCCATAG

Protein sequence predicted based on nucleotide sequence

MLDAFQREAIQCVDNNQSVLVSAHTSAGKTVCAEYAIALALREKQRVIFTSPIKALSNQK
YREMYEEFQDVGLMTGDVTINPTASCLVMTTEILRSMLYRGSEVMREVAWVIFDEIHYMR
DSERGVVWEETIILLPDNVHYVFLSATIPNARQFAEWICHLHKQPCHVIYTDYRPTPLQH
YIFPAGGDGLHLVVDENGDFREDNFNTAMQVLRDAGDLAKGDQKGRKGGTKGPSNVFKIV
KMIMERNFQPVIIFSFSKKDCEAYALQMTKLDFNTDEEKKMVEEVFSNAIDCLSDEDKKL
PQVEHVLPLLKRGIGIHHGGLLPILKETIEILFSEGLIKALFATETFAMGINMPARTVLF
TNARKFDGKDFRWISSGEYIQMSGRAGRRGMDDRGIVILMVDEKMSP*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

g837a,K279K

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinPurified: Not_Tested

ProteinExpressed: Protein_Confirmed

SolubleProtein: Tested_Not_Soluble

Find experimental protocols in HR9170B

Recommended expression in:

Not Applicable

Vector Name:

pET15_NESG

Synonyms:

pET15, pET15-modified, pET15Cc, pET15_NESG

Sequencing Primer:

Forward: T7

Reverse: T7 terminator

Description:

Bacterial (E. coli) expression vector with T7 lac promoter, adds N-terminal 6x His tag; amp resistance; restriction enzyme cloning.

Comments:

Modified Novagen pET vector-common MCS

Size (bp):

5723

Parent Vector:

None

Properties:

bacterial expression, in vitro transcription, multiple cloning site, with tag/fusion/marker

Author Name:

Gaetano T. Montleione
Jessica Bearden
Northeast Structural Genomics Consortium

Publications:

PMID: 15808222

Title: Robotic cloning and Protein Production Platform of the Northeast Structural Genomics Consortium

Vector Map:

Vector Sequence:

Sequence:

TTCTCATGTTTGACAGCTTATCATCGATAAGCTAGCTTTAATGCGGTAGTTTATCACAGTTAAATTGCTAACGCAGTCAGGCACCGTGTATGAAATCTAACAATGCGCTCATCGTCATCCTCGGCACCGTCACCCTGGATGCTGTAGGCATAGGCTTGGTTATGCCGGTACTGCCGGGCCTCTTGCGGGATATCCGGATATAGTTCCTCCTTTCAGCAAAAAACCCCTCAAGACCCGTTTAGAGGCCCCAAGGGGTTATGCTAGTTATTGCTCAGCGGTGGCAGCAGCCAACTCAGCTTCCTTTCGGGCTTTGTTAGCAGCCGGATCTCGAGAAGCTTTGCGTCGACAGAGCTCGCAGGATCCGCAGAATTCGCCATATGGCTGTGATGGTGATGGTGATGGCCCATGGTATATCTCCTTCTTAAAGTTAAACAAAATTATTTCTAGAGGGGAATTGTTATCCGCTCACAATTCCCCTATAGTGAGTCGTATTAATTTCGCGGGATCGAGATCTCGATCCTCTACGCCGGACGCATCGTGGCCGGCATCACCGGCGCCACAGGTGCGGTTGCTGGCGCCTATATCGCCGACATCACCGATGGGGAAGATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTGTTTCGGCGTGGGTATGGTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCTTGCATGCACCATTCCTTGCGGCGGCGGTGCTCAACGGCCTCAACCTACTACTGGGCTGCTTCCTAATGCAGGAGTCGCATAAGGGAGAGCGTCGAGATCCCGGACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAATGTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAATGCTGAATGAGGGCATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTGCGGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTTATATCCCGCCGTTAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTAAGTTAGCTCACTCATTAGGCACCGGGATCTCGACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACTATCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGCGCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCCTTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTCCCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTGTCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACCAGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGCCTCGGCGAGCACATGGAACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGTCGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACGGATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAACCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCATCTCGGGCAGCGTTGGGTCCTGGCCACGGGTGCGCATGATCGTGCTCCTGTCGTTGAGGACCCGGCTAGGCTGGCGGGGTTGCCTTACTGGTTAGCAGAATGAATCACCGATACGCGAGCGAACGTGAAGCGACTGCTGCTGCAAAACGTCTGCGACCTGAGCAACAACATGAATGGTCTTCGGTTTCCGTGTTTCGTAAAGTCTGGAAACGCGGAAGTCAGCGCCCTGCACCATTATGTTCCGGATCTGCATCGCAGGATGCTGCTGGCTACCCTGTGGAACACCTACATCTGTATTAACGAAGCGCTGGCATTGACCCTGAGTGATTTTTCTCTGGTCCCGCCGCATCCATACCGCCAGTTGTTTACCCTCACAACGTTCCAGTAACCGGGCATGTTCATCATCAGTAACCCGTATCGTGAGCATCCTCTCTCGTTTCATCGGTATCATTACCCCCATGAACAGAAATCCCCCTTACACGGAGGCATCAGTGACCAAACAGGAAAAAACCGCCCTTAACATGGCCCGCTTTATCAGAAGCCAGACATTAACGCTTCTGGAGAAACTCAACGAGCTGGACGCGGATGAACAGGCAGACATCTGTGAATCGCTTCACGACCACGCTGATGAGCTTTACCGCAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTGCAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAACACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTTCAAGAA

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (includes SphI, XbaI, EcoRI, BamHI, XhoI)

327

452

bacterial origin

ori

ColE1 origin of replication

3881

4563

gene fragment

ROP

repressor of primer ROP sequence

3281

3457

primer

T7 forward

T7 forward sequencing primer

481

496

primer

T7 reverse

T7 reverse sequencing primer

257

275

promoter

T7 promoter

478

497

repressor binding site

lacO

lacO regulatory sequence

456

478

selectable marker

AmpR

ampicillin resistance gene

4661

5230

tag

His

N-terminal 6x His tag

387

404

trxn termination sequence

T7 term

T7 termination sequence

257

275

Gene

Gene Symbol :

SKIV2L2

Symbol Nomenclature :

SKIV2L2

SYNONYM :

Dob1; KIAA0052; Mtr4; fSAP118

Designation :

ATP-dependent helicase SKIV2L2|functional spliceosome-associated protein 118|superkiller viralicidic activity 2-like 2

Full Nomenclature :

superkiller viralicidic activity 2-like 2 (S. cerevisiae)

GENEID :

23517

GI :

193211480

GenBank Accession :

NM_015360

HGNC :

18734

Vega :

OTTHUMG00000097019

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00583776      ------------------------------------------------------------
NM_015360.4       ATGGCGGACGCATTCGGAGATGAGCTGTTCAGCGTGTTCGAGGGCGACTCGACCACTGCG
                                                                              

HsCD00583776      ------------------------------------------------------------
NM_015360.4       GCGGGAACCAAAAAAGACAAGGAAAAGGACAAGGGGAAATGGAAGGGGCCTCCAGGGTCT
                                                                              

HsCD00583776      ------------------------------------------------------------
NM_015360.4       GCAGACAAGGCAGGGAAACGTTTTGATGGTAAATTACAATCAGAATCAACTAATAATGGA
                                                                              

HsCD00583776      ------------------------------------------------------------
NM_015360.4       AAAAATAAGAGAGATGTAGATTTCGAAGGTACAGATGAACCCATTTTTGGAAAGAAGCCC
                                                                              

HsCD00583776      ------------------------------------------------------------
NM_015360.4       AGGATAGAAGAGTCAATAACTGAAGACTTAAGTTTGGCAGACCTGATGCCCAGAGTCAAG
                                                                              

HsCD00583776      ------------------------------------------------------------
NM_015360.4       GTACAATCAGTTGAAACTGTTGAAGGGTGTACACATGAGGTTGCACTTCCTGCAGAAGAG
                                                                              

HsCD00583776      ----------------------------------------------------ATATGCTT
NM_015360.4       GATTATTTACCACTTAAACCACGAGTTGGAAAAGCTGCTAAGGAATACCCGTTCATTCTT
                                                                      : ** ***

HsCD00583776      GATGCTTTTCAAAGAGAGGCCATTCAGTGTGTTGACAATAATCAGTCTGTTCTAGTATCT
NM_015360.4       GATGCTTTTCAAAGAGAGGCCATTCAGTGTGTTGACAATAATCAGTCTGTTCTAGTATCT
                  ************************************************************

HsCD00583776      GCACATACCTCAGCGGGAAAAACAGTATGCGCCGAGTATGCCATTGCATTGGCCTTAAGG
NM_015360.4       GCACATACCTCAGCGGGAAAAACAGTATGCGCCGAGTATGCCATTGCATTGGCCTTAAGG
                  ************************************************************

HsCD00583776      GAAAAGCAGCGTGTAATATTTACCAGCCCAATTAAGGCTCTGAGTAACCAAAAATACCGT
NM_015360.4       GAAAAGCAGCGTGTAATATTTACCAGCCCAATTAAGGCTCTGAGTAACCAAAAATACCGT
                  ************************************************************

HsCD00583776      GAAATGTATGAAGAATTTCAAGATGTTGGTTTGATGACTGGTGATGTTACTATTAATCCT
NM_015360.4       GAAATGTATGAAGAATTTCAAGATGTTGGTTTGATGACTGGTGATGTTACTATTAATCCT
                  ************************************************************

HsCD00583776      ACGGCATCTTGTCTTGTTATGACCACAGAGATTTTGAGAAGTATGCTTTACAGAGGTTCC
NM_015360.4       ACGGCATCTTGTCTTGTTATGACCACAGAGATTTTGAGAAGTATGCTTTACAGAGGTTCC
                  ************************************************************

HsCD00583776      GAAGTTATGAGAGAAGTTGCTTGGGTTATATTTGATGAAATTCATTATATGAGAGATTCA
NM_015360.4       GAAGTTATGAGAGAAGTTGCTTGGGTTATATTTGATGAAATTCATTATATGAGAGATTCA
                  ************************************************************

HsCD00583776      GAACGTGGTGTAGTATGGGAAGAAACTATTATTTTGCTTCCTGATAACGTCCACTATGTC
NM_015360.4       GAACGTGGTGTAGTATGGGAAGAAACTATTATTTTGCTTCCTGATAACGTCCACTATGTC
                  ************************************************************

HsCD00583776      TTTCTTTCGGCTACTATTCCAAATGCCCGACAGTTTGCTGAATGGATTTGCCATTTACAT
NM_015360.4       TTTCTTTCGGCTACTATTCCAAATGCCCGACAGTTTGCTGAATGGATTTGCCATTTACAT
                  ************************************************************

HsCD00583776      AAACAGCCTTGTCATGTTATTTACACAGATTATCGGCCCACTCCATTGCAACACTACATT
NM_015360.4       AAACAGCCTTGTCATGTTATTTACACAGATTATCGGCCCACTCCATTGCAACACTACATT
                  ************************************************************

HsCD00583776      TTTCCAGCAGGGGGAGATGGCCTGCATCTTGTGGTTGATGAAAATGGTGACTTCAGAGAA
NM_015360.4       TTTCCAGCAGGGGGAGATGGCCTGCATCTTGTGGTTGATGAAAATGGTGACTTCAGAGAA
                  ************************************************************

HsCD00583776      GATAATTTTAATACTGCAATGCAAGTGCTTCGAGATGCAGGTGATTTGGCCAAAGGAGAC
NM_015360.4       GATAATTTTAATACTGCAATGCAAGTGCTTCGAGATGCAGGTGATTTGGCCAAAGGAGAC
                  ************************************************************

HsCD00583776      CAGAAAGGGCGGAAAGGAGGAACAAAAGGACCATCAAATGTTTTCAAAATTGTGAAGATG
NM_015360.4       CAGAAAGGGCGGAAAGGAGGAACAAAAGGACCATCAAATGTTTTCAAAATTGTGAAGATG
                  ************************************************************

HsCD00583776      ATTATGGAAAGAAATTTCCAACCTGTGATTATTTTCAGTTTTAGTAAGAAAGATTGTGAA
NM_015360.4       ATTATGGAAAGAAATTTCCAACCTGTGATTATTTTCAGTTTTAGTAAGAAAGATTGTGAA
                  ************************************************************

HsCD00583776      GCCTATGCACTTCAAATGACCAAATTAGATTTCAACACAGATGAAGAAAAAAAGATGGTT
NM_015360.4       GCCTATGCACTTCAAATGACCAAATTAGATTTCAACACAGATGAAGAAAAGAAGATGGTT
                  **************************************************.*********

HsCD00583776      GAAGAAGTATTCAGTAATGCAATTGATTGCTTATCCGATGAAGATAAAAAACTCCCTCAG
NM_015360.4       GAAGAAGTATTCAGTAATGCAATTGATTGCTTATCCGATGAAGATAAAAAACTCCCTCAG
                  ************************************************************

HsCD00583776      GTAGAACATGTACTTCCTCTTTTGAAGAGGGGAATTGGTATTCACCATGGTGGTTTACTT
NM_015360.4       GTAGAACATGTACTTCCTCTTTTGAAGAGGGGAATTGGTATTCACCATGGTGGTTTACTT
                  ************************************************************

HsCD00583776      CCTATTTTGAAAGAAACTATAGAAATTCTCTTTTCTGAAGGATTGATAAAGGCCTTATTT
NM_015360.4       CCTATTTTGAAAGAAACTATAGAAATTCTCTTTTCTGAAGGATTGATAAAGGCCTTATTT
                  ************************************************************

HsCD00583776      GCCACGGAGACCTTTGCTATGGGAATTAACATGCCAGCTAGAACTGTTTTATTTACAAAT
NM_015360.4       GCCACGGAGACCTTTGCTATGGGAATTAACATGCCAGCTAGAACTGTTTTATTTACAAAT
                  ************************************************************

HsCD00583776      GCCCGCAAATTTGATGGGAAGGATTTCCGATGGATTTCTTCTGGTGAATACATTCAGATG
NM_015360.4       GCCCGCAAATTTGATGGGAAGGATTTCCGATGGATTTCTTCTGGTGAATACATTCAGATG
                  ************************************************************

HsCD00583776      TCTGGTCGTGCTGGAAGGAGAGGAATGGATGATAGAGGAATTGTAATTCTTATGGTAGAT
NM_015360.4       TCTGGTCGTGCTGGAAGGAGAGGAATGGATGATAGAGGAATTGTAATTCTTATGGTAGAT
                  ************************************************************

HsCD00583776      GAAAAGATGAGCCCATAG------------------------------------------
NM_015360.4       GAAAAGATGAGCCCAACAATTGGAAAACAATTACTTAAGGGCTCCGCTGATCCTCTAAAT
                  ***************:..                                          

HsCD00583776      ------------------------------------------------------------
NM_015360.4       AGTGCTTTCCATTTGACCTACAACATGGTTTTGAACTTACTACGTGTAGAAGAAATTAAT
                                                                              

HsCD00583776      ------------------------------------------------------------
NM_015360.4       CCTGAGTACATGTTGGAAAAATCCTTCTACCAGTTTCAGCATTATAGAGCAATTCCAGGA
                                                                              

HsCD00583776      ------------------------------------------------------------
NM_015360.4       GTAGTAGAGAAGGTAAAGAATTCAGAAGAACAGTATAATAAAATAGTAATTCCCAATGAA
                                                                              

HsCD00583776      ------------------------------------------------------------
NM_015360.4       GAAAGTGTGGTTATCTATTATAAGATTAGACAGCAGCTTGCCAAATTGGGTAAAGAAATT
                                                                              

HsCD00583776      ------------------------------------------------------------
NM_015360.4       GAAGAATATATTCACAAACCAAAATACTGCTTACCTTTTCTACAACCAGGTCGTTTGGTA
                                                                              

HsCD00583776      ------------------------------------------------------------
NM_015360.4       AAGGTAAAGAATGAAGGAGATGACTTTGGCTGGGGAGTAGTGGTGAATTTCTCAAAAAAG
                                                                              

HsCD00583776      ------------------------------------------------------------
NM_015360.4       TCAAATGTTAAGCCTAACTCTGGTGAACTGGATCCTTTGTATGTAGTAGAAGTACTTCTG
                                                                              

HsCD00583776      ------------------------------------------------------------
NM_015360.4       CGCTGTAGCAAAGAGAGCTTGAAAAATTCAGCTACAGAAGCTGCAAAACCAGCTAAACCT
                                                                              

HsCD00583776      ------------------------------------------------------------
NM_015360.4       GATGAGAAAGGAGAGATGCAGGTTGTCCCAGTTTTGGTGCATCTCCTGTCTGCTATCAGC
                                                                              

HsCD00583776      ------------------------------------------------------------
NM_015360.4       AGTGTTAGGCTTTACATTCCTAAAGACCTTCGGCCGGTGGACAATAGACAGAGTGTTTTA
                                                                              

HsCD00583776      ------------------------------------------------------------
NM_015360.4       AAATCAATACAGGAAGTTCAGAAACGTTTTCCTGACGGCATCCCCTTATTAGACCCTATT
                                                                              

HsCD00583776      ------------------------------------------------------------
NM_015360.4       GATGATATGGGCATTCAAGATCAAGGGCTGAAAAAAGTCATTCAGAAAGTAGAAGCTTTT
                                                                              

HsCD00583776      ------------------------------------------------------------
NM_015360.4       GAGCATCGAATGTATTCTCATCCACTTCACAATGATCCAAATTTGGAAACTGTGTATACG
                                                                              

HsCD00583776      ------------------------------------------------------------
NM_015360.4       CTTTGTGAAAAAAAAGCACAGATTGCAATAGATATTAAATCTGCAAAGCGAGAACTGAAG
                                                                              

HsCD00583776      ------------------------------------------------------------
NM_015360.4       AAAGCAAGAACAGTCCTACAAATGGATGAACTCAAATGTCGCAAACGTGTTTTAAGAAGG
                                                                              

HsCD00583776      ------------------------------------------------------------
NM_015360.4       TTGGGATTTGCTACTTCTTCTGATGTAATAGAGATGAAAGGACGAGTGGCTTGTGAGATA
                                                                              

HsCD00583776      ------------------------------------------------------------
NM_015360.4       AGCAGTGCTGATGAGCTCCTTCTAACTGAGATGATGTTTAATGGCCTTTTCAATGACCTT
                                                                              

HsCD00583776      ------------------------------------------------------------
NM_015360.4       TCTGCAGAACAGGCAACAGCATTATTAAGCTGCTTTGTGTTTCAAGAGAATTCTAGTGAG
                                                                              

HsCD00583776      ------------------------------------------------------------
NM_015360.4       ATGCCCAAATTAACAGAACAATTAGCAGGACCACTTCGTCAAATGCAGGAATGTGCTAAA
                                                                              

HsCD00583776      ------------------------------------------------------------
NM_015360.4       AGAATTGCAAAAGTTTCAGCAGAAGCCAAATTGGAAATTGATGAGGAAACTTATCTAAGC
                                                                              

HsCD00583776      ------------------------------------------------------------
NM_015360.4       TCATTTAAACCTCACTTAATGGATGTAGTATATACCTGGGCAACTGGAGCTACATTTGCC
                                                                              

HsCD00583776      ------------------------------------------------------------
NM_015360.4       CATATCTGCAAAATGACAGATGTCTTTGAAGGCAGCATAATTCGTTGTATGAGGCGCCTG
                                                                              

HsCD00583776      ------------------------------------------------------------
NM_015360.4       GAAGAATTGCTTCGACAAATGTGTCAAGCAGCAAAAGCCATTGGAAACACTGAGCTGGAA
                                                                              

HsCD00583776      ------------------------------------------------------------
NM_015360.4       AATAAATTTGCAGAAGGAATCACCAAAATCAAGAGAGATATTGTGTTTGCTGCCAGCCTC
                                                                              

HsCD00583776      ---------
NM_015360.4       TACTTGTAG