DNASU Plasmid | RFC5 (H. sapiens )<br>In pET15_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: RFC5

Gene Name: replication factor C (activator 1) 5, 36.5kDa

Original Clone ID:

HR3045B-15-257-15.3

Keyword:

targeted domain

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pET15_NESG

Format:

CLOSED

Source:

Northeast Structural Genomics Consortium

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

PSI:Biology
Northeast Structural Genomics Consortium

HsCD00583837

Price:

AVAILABLE

No restriction
Special MTA: PSI/NESG

Insert Sequence
Protein Sequence

Insert sequence: 735nts

Open reading frame: 50 to 784

Reference Sequence Alignment

CTTAAGAAGGAGATATACCATGGGCCATCACCATCACCATCACAGCCATATGACCAAGAT
CAGGAACCTGCCCTGGGTTGAAAAATACCGGCCACAGACCCTGAATGATCTCATTTCTCA
TCAGGACATTCTGAGTACCATTCAGAAGTTTATCAATGAAGACCGACTGCCACACTTGCT
TCTCTACGGTCCCCCAGGGACAGGCAAGACATCTACCATCCTAGCCTGTGCGAAACAGCT
ATATAAAGACAAAGAATTTGGCTCCATGGTCTTGGAGCTGAATGCTTCAGATGACCGAGG
AATAGACATCATTCGAGGACCGATCCTGAGCTTTGCTAGCACAAGGACAATATTTAAGAA
AGGCTTTAAGCTAGTGATCTTGGATGAAGCAGACGCCATGACTCAGGACGCCCAGAATGC
CTTGAGAAGAGTAATTGAGAAATTCACAGAAAATACCAGATTCTGCCTCATCTGTAACTA
TCTGTCAAAGATCATCCCTGCCTTGCAGTCCCGCTGCACGAGGTTTCGGTTCGGTCCCCT
GACTCCTGAACTCATGGTTCCCCGCCTGGAACATGTCGTGGAAGAAGAGAAAGTTGATAT
AAGTGAAGATGGAATGAAAGCACTAGTCACTCTTTCCAGTGGAGACATGCGTAGGGCTCT
GAACATTTTGCAGAGCACCAATATGGCCTTTGGGAAGGTGACAGAGGAGACTGTCTACAC
CTGCACCGGGCACCCGCTCAAGTCAGACATTGCCAACATCCTGGACTGGATGTTGAATCA
ATAGCTCGAGATCCGGCTGCTAA

Protein sequence predicted based on nucleotide sequence

MTKIRNLPWVEKYRPQTLNDLISHQDILSTIQKFINEDRLPHLLLYGPPGTGKTSTILAC
AKQLYKDKEFGSMVLELNASDDRGIDIIRGPILSFASTRTIFKKGFKLVILDEADAMTQD
AQNALRRVIEKFTENTRFCLICNYLSKIIPALQSRCTRFRFGPLTPELMVPRLEHVVEEE
KVDISEDGMKALVTLSSGDMRRALNILQSTNMAFGKVTEETVYTCTGHPLKSDIANILDW
MLNQ*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinPurified: Not_Tested

SolubleProtein: Not_Tested

ProteinExpressed: Tested_Not_Found

Find experimental protocols in HR3045B

Recommended expression in:

Not Applicable

Vector Name:

pET15_NESG

Synonyms:

pET15, pET15-modified, pET15Cc, pET15_NESG

Sequencing Primer:

Forward: T7

Reverse: T7 terminator

Description:

Bacterial (E. coli) expression vector with T7 lac promoter, adds N-terminal 6x His tag; amp resistance; restriction enzyme cloning.

Comments:

Modified Novagen pET vector-common MCS

Size (bp):

5723

Parent Vector:

None

Properties:

bacterial expression, in vitro transcription, multiple cloning site, with tag/fusion/marker

Author Name:

Gaetano T. Montleione
Jessica Bearden
Northeast Structural Genomics Consortium

Publications:

PMID: 15808222

Title: Robotic cloning and Protein Production Platform of the Northeast Structural Genomics Consortium

Vector Map:

Vector Sequence:

Sequence:

TTCTCATGTTTGACAGCTTATCATCGATAAGCTAGCTTTAATGCGGTAGTTTATCACAGTTAAATTGCTAACGCAGTCAGGCACCGTGTATGAAATCTAACAATGCGCTCATCGTCATCCTCGGCACCGTCACCCTGGATGCTGTAGGCATAGGCTTGGTTATGCCGGTACTGCCGGGCCTCTTGCGGGATATCCGGATATAGTTCCTCCTTTCAGCAAAAAACCCCTCAAGACCCGTTTAGAGGCCCCAAGGGGTTATGCTAGTTATTGCTCAGCGGTGGCAGCAGCCAACTCAGCTTCCTTTCGGGCTTTGTTAGCAGCCGGATCTCGAGAAGCTTTGCGTCGACAGAGCTCGCAGGATCCGCAGAATTCGCCATATGGCTGTGATGGTGATGGTGATGGCCCATGGTATATCTCCTTCTTAAAGTTAAACAAAATTATTTCTAGAGGGGAATTGTTATCCGCTCACAATTCCCCTATAGTGAGTCGTATTAATTTCGCGGGATCGAGATCTCGATCCTCTACGCCGGACGCATCGTGGCCGGCATCACCGGCGCCACAGGTGCGGTTGCTGGCGCCTATATCGCCGACATCACCGATGGGGAAGATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTGTTTCGGCGTGGGTATGGTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCTTGCATGCACCATTCCTTGCGGCGGCGGTGCTCAACGGCCTCAACCTACTACTGGGCTGCTTCCTAATGCAGGAGTCGCATAAGGGAGAGCGTCGAGATCCCGGACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAATGTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAATGCTGAATGAGGGCATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTGCGGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTTATATCCCGCCGTTAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTAAGTTAGCTCACTCATTAGGCACCGGGATCTCGACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACTATCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGCGCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCCTTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTCCCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTGTCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACCAGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGCCTCGGCGAGCACATGGAACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGTCGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACGGATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAACCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCATCTCGGGCAGCGTTGGGTCCTGGCCACGGGTGCGCATGATCGTGCTCCTGTCGTTGAGGACCCGGCTAGGCTGGCGGGGTTGCCTTACTGGTTAGCAGAATGAATCACCGATACGCGAGCGAACGTGAAGCGACTGCTGCTGCAAAACGTCTGCGACCTGAGCAACAACATGAATGGTCTTCGGTTTCCGTGTTTCGTAAAGTCTGGAAACGCGGAAGTCAGCGCCCTGCACCATTATGTTCCGGATCTGCATCGCAGGATGCTGCTGGCTACCCTGTGGAACACCTACATCTGTATTAACGAAGCGCTGGCATTGACCCTGAGTGATTTTTCTCTGGTCCCGCCGCATCCATACCGCCAGTTGTTTACCCTCACAACGTTCCAGTAACCGGGCATGTTCATCATCAGTAACCCGTATCGTGAGCATCCTCTCTCGTTTCATCGGTATCATTACCCCCATGAACAGAAATCCCCCTTACACGGAGGCATCAGTGACCAAACAGGAAAAAACCGCCCTTAACATGGCCCGCTTTATCAGAAGCCAGACATTAACGCTTCTGGAGAAACTCAACGAGCTGGACGCGGATGAACAGGCAGACATCTGTGAATCGCTTCACGACCACGCTGATGAGCTTTACCGCAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTGCAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAACACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTTCAAGAA

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (includes SphI, XbaI, EcoRI, BamHI, XhoI)

327

452

bacterial origin

ori

ColE1 origin of replication

3881

4563

gene fragment

ROP

repressor of primer ROP sequence

3281

3457

primer

T7 forward

T7 forward sequencing primer

481

496

primer

T7 reverse

T7 reverse sequencing primer

257

275

promoter

T7 promoter

478

497

repressor binding site

lacO

lacO regulatory sequence

456

478

selectable marker

AmpR

ampicillin resistance gene

4661

5230

tag

His

N-terminal 6x His tag

387

404

trxn termination sequence

T7 term

T7 termination sequence

257

275

Gene

Gene Symbol :

RFC5

Symbol Nomenclature :

RFC5

SYNONYM :

RFC36

Designation :

A1 36 kDa subunit|RF-C 36 kDa subunit|RFC, 36.5 kD subunit|replication factor C subunit 5|replication factor C, 36-kDa subunit

Full Nomenclature :

replication factor C (activator 1) 5, 36.5kDa

GENEID :

5985

GenBank Accession :

NM_001130113

HGNC :

9973

MIM :

600407

Vega :

OTTHUMG00000156438

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00583837        CTTAAGAAGGAGATATACCATGGGCCATCACCATCACCATCACAGCCATATGACCAAGAT
NM_001130112.2      ------------------------------------------------------------
NM_007370.5         ----------ATGGAGACCTCAGCACTCAAGCAGCAGGAGCAGCCCGCGGCGACCAAGAT
NM_181578.3         ------------------------------------------------------------
NM_001206801.1      ----------ATGGAGACCTCAGCACTCAAGCAGCAGGAGCAGCCCGCGGCGACCAAGAT
                                                                                

HsCD00583837        CAGGAACCTGCCCTGGGTTGAAAAATACCGGCCACAGACCCTGAATGATCTCATTTCTCA
NM_001130112.2      ------------------------------------------------------------
NM_007370.5         CAGGAACCTGCCCTGGGTTGAAAAATACCGGCCACAGACCCTGAATGATCTCATTTCTCA
NM_181578.3         -------------ATGGTTGAAAAATACCGGCCACAGACCCTGAATGATCTCATTTCTCA
NM_001206801.1      CAGGAACCTGCCCTGGGTTGAAAAATACCGGCCACAGACCCTGAATGATCTCATTTCTCA
                                                                                

HsCD00583837        TCAGGACATTCTGAGTACCATTCAGAAGTTTATCAATGAAGACCGACTGCCACACTTGCT
NM_001130112.2      ------------------------------------------------------------
NM_007370.5         TCAGGACATTCTGAGTACCATTCAGAAGTTTATCAATGAAGACCGACTGCCACACTTGCT
NM_181578.3         TCAGGACATTCTGAGTACCATTCAGAAGTTTATCAATGAAGACCGACTGCCACACTTGCT
NM_001206801.1      TCAGGACATTCTGAGTACCATTCAGAAGTTTATCAATGAAGACCGACTGCCACACTTGCT
                                                                                

HsCD00583837        TCTCTACGGTCCCCCAGGGACAGGCAAGACATCTACCATCCTAGCCTGTGCGAAACAGCT
NM_001130112.2      ------------------------------------------------------------
NM_007370.5         TCTCTACGGTCCCCCAGGGACAGGCAAGACATCTACCATCCTAGCCTGTGCGAAACAGCT
NM_181578.3         TCTCTACGGTCCCCCAGGGACAGGCAAGACATCTACCATCCTAGCCTGTGCGAAACAGCT
NM_001206801.1      TCTCTACGGTCCCCCAGGGACAGGCAAGACATCTACCATCCTAGCCTGTGCGAAACAGCT
                                                                                

HsCD00583837        ATATAAAGACAAAGAATTTGGCTCCATGGTCTTGGAGCTGAATGCTTCAGATGACCGAGG
NM_001130112.2      -------------------------ATGGTCTTGGAGCTGAATGCTTCAGATGACCGAGG
NM_007370.5         ATATAAAGACAAAGAATTTGGCTCCATGGTCTTGGAGCTGAATGCTTCAGATGACCGAGG
NM_181578.3         ATATAAAGACAAAGAATTTGGCTCCATGGTCTTGGAGCTGAATGCTTCAGATGACCGAGG
NM_001206801.1      ATATAAAGACAAAGAATTTGGCTCCATGGTCTTGGAGCTGAATGCTTCAGATGACCGAGG
                                             ***********************************

HsCD00583837        AATAGACATCATTCGAGGACCGATCCTGAGCTTTGCTAGCACAAGGACAATATTTAAGAA
NM_001130112.2      AATAGACATCATTCGAGGACCGATCCTGAGCTTTGCTAGCACAAGGACAATATTTAAGAA
NM_007370.5         AATAGACATCATTCGAGGACCGATCCTGAGCTTTGCTAGCACAAGGACAATATTTAAGAA
NM_181578.3         AATAGACATCATTCGAGGACCGATCCTGAGCTTTGCTAGCACAAGGACAATATTTAAGAA
NM_001206801.1      AATAGACATCATTCGAGGACCGATCCTGAGCTTTGCTAGCACAAGGACAATATTTAAGAA
                    ************************************************************

HsCD00583837        AGGCTTTAAGCTAGTGATCTTGGATGAAGCAGACGCCATGACTCAGGACGCCCAGAATGC
NM_001130112.2      AGGCTTTAAGCTAGTGATCTTGGATGAAGCAGACGCCATGACTCAGGACGCCCAGAATGC
NM_007370.5         AGGCTTTAAGCTAGTGATCTTGGATGAAGCAGACGCCATGACTCAGGACGCCCAGAATGC
NM_181578.3         AGGCTTTAAGCTAGTGATCTTGGATGAAGCAGACGCCATGACTCAGGACGCCCAGAATGC
NM_001206801.1      AGGCTTTAAGCTAGTGATCTTGGATGAAGCAGACGCCATGACTCAGGACGCCCAGAATGC
                    ************************************************************

HsCD00583837        CTTGAGAAGAGTAATTGAGAAATTCACAGAAAATACCAGATTCTGCCTCATCTGTAACTA
NM_001130112.2      CTTGAGAAGAGTAATTGAGAAATTCACAGAAAATACCAGATTCTGCCTCATCTGTAACTA
NM_007370.5         CTTGAGAAGAGTAATTGAGAAATTCACAGAAAATACCAGATTCTGCCTCATCTGTAACTA
NM_181578.3         CTTGAGAAGAGTAATTGAGAAATTCACAGAAAATACCAGATTCTGCCTCATCTGTAACTA
NM_001206801.1      CTTGAGAAGAGTAATTGAGAAATTCACAGAAAATACCAGATTCTGCCTCATCTGTAACTA
                    ************************************************************

HsCD00583837        TCTGTCAAAGATCATCCCTGCCTTGCAGTCCCGCTGCACGAGGTTTCGGTTCGGTCCCCT
NM_001130112.2      TCTGTCAAAGATCATCCCTGCCTTGCAGTCCCGCTGCACGAGGTTTCGGTTCGGTCCCCT
NM_007370.5         TCTGTCAAAGATCATCCCTGCCTTGCAGTCCCGCTGCACGAGGTTTCGGTTCGGTCCCCT
NM_181578.3         TCTGTCAAAGATCATCCCTGCCTTGCAGTCCCGCTGCACGAGGTTTCGGTTCGGTCCCCT
NM_001206801.1      TCTGTCAAAGATCATCCCTGCCTTGCAGTCCCGCTGCACGAGGTTTCGGTTCGGTCCCCT
                    ************************************************************

HsCD00583837        GACTCCTGAACTCATGGTTCCCCGCCTGGAACATGTCGTGGAAGAAGAGAAAGTTGATAT
NM_001130112.2      GACTCCTGAACTCATGGTTCCCCGCCTGGAACATGTCGTGGAAGAAGAGAAAGTTGATAT
NM_007370.5         GACTCCTGAACTCATGGTTCCCCGCCTGGAACATGTCGTGGAAGAAGAGAAAGTTGATAT
NM_181578.3         GACTCCTGAACTCATGGTTCCCCGCCTGGAACATGTCGTGGAAGAAGAGAAAGTTGATAT
NM_001206801.1      GACTCCTGAACTCATGGTTCCCCGCCTGGAACATGTCGTGGAAGAAGAGAAAGTTGATAT
                    ************************************************************

HsCD00583837        AAGTGAAGATGGAATGAAAGCACTAGTCACTCTTTCCAGTGGAGACATGCGTAGGGCTCT
NM_001130112.2      AAGTGAAGATGGAATGAAAGCACTAGTCACTCTTTCCAGTGGAGACATGCGTAGGGCTCT
NM_007370.5         AAGTGAAGATGGAATGAAAGCACTAGTCACTCTTTCCAGTGGAGACATGCGTAGGGCTCT
NM_181578.3         AAGTGAAGATGGAATGAAAGCACTAGTCACTCTTTCCAGTGGAGACATGCGTAGGGCTCT
NM_001206801.1      AAGTGAAGATGGAATGAAAGCACTAGTCACTCTTTCCAGTGGAGACATGCGTAGGGCTCT
                    ************************************************************

HsCD00583837        GAACATTTTGCAGAGCACCAATATGGCCTTTGGGAAGGTGACAGAGGAGACTGTCTACAC
NM_001130112.2      GAACATTTTGCAGAGCACCAATATGGCCTTTGGGAAGGTGACAGAGGAGACTGTCTACAC
NM_007370.5         GAACATTTTGCAGAGCACCAATATGGCCTTTGGGAAGGTGACAGAGGAGACTGTCTACAC
NM_181578.3         GAACATTTTGCAGAGCACCAATATGGCCTTTGGGAAGGTGACAGAGGAGACTGTCTACAC
NM_001206801.1      GAACATTTTGCAGAGCACCAATATGGCCTTTGGGAAGGTGACAGAGGAGACTGTCTACAC
                    ************************************************************

HsCD00583837        CTGCACCGGGCACCCGCTCAAGTCAGACATTGCCAACATCCTGGACTGGATGTTGAATCA
NM_001130112.2      CTGCACCGGGCACCCGCTCAAGTCAGACATTGCCAACATCCTGGACTGGATGTTGAATCA
NM_007370.5         CTGCACCGGGCACCCGCTCAAGTCAGACATTGCCAACATCCTGGACTGGATGTTGAATCA
NM_181578.3         CTGCACCGGGCACCCGCTCAAGTCAGACATTGCCAACATCCTGGACTGGATGTTGAATCA
NM_001206801.1      CTGCACCGGGCACCCGCTCAAGTCAGACATTGCCAACATCCTGGACTGGATGTTGAATCA
                    ************************************************************

HsCD00583837        ATAGCTCGAGATCCGGCTGCTAA-------------------------------------
NM_001130112.2      AGATTTCACCACAGCCTACAGAAATATTACAGAGTTGAAAACTCTGAAGGGGTTGGCACT
NM_007370.5         AGATTTCACCACAGCCTACAGAAATATTACAGAGTTGAAAACTCTGAAGGGGTTGGCACT
NM_181578.3         AGATTTCACCACAGCCTACAGAAATATTACAGAGTTGAAAACTCTGAAGGGGTTGGCACT
NM_001206801.1      AGATTTCACCACAGCCTACAGAAAGTTGAAAACTCTGAAGGGGTTGGCACTGCATGATAT
                    * *  **.. * .    : . **                                     

HsCD00583837        ------------------------------------------------------------
NM_001130112.2      GCATGATATCCTGACAGAGATACACTTGTTTGTGCATAGAGTTGACTTTCCATCTTCAGT
NM_007370.5         GCATGATATCCTGACAGAGATACACTTGTTTGTGCATAGAGTTGACTTTCCATCTTCAGT
NM_181578.3         GCATGATATCCTGACAGAGATACACTTGTTTGTGCATAGAGTTGACTTTCCATCTTCAGT
NM_001206801.1      CCTGACAGAGATACACTTGTTTGTGCATAGAGTTGACTTTCCATCTTCAGTTCGAATACA
                                                                                

HsCD00583837        ------------------------------------------------------------
NM_001130112.2      TCGAATACATTTATTGACCAAAATGGCAGACATTGAGTACAGGCTTTCTGTTGGCACCAA
NM_007370.5         TCGAATACATTTATTGACCAAAATGGCAGACATTGAGTACAGGCTTTCTGTTGGCACCAA
NM_181578.3         TCGAATACATTTATTGACCAAAATGGCAGACATTGAGTACAGGCTTTCTGTTGGCACCAA
NM_001206801.1      TTTATTGACCAAAATGGCAGACATTGAGTACAGGCTTTCTGTTGGCACCAACGAGAAGAT
                                                                                

HsCD00583837        ------------------------------------------------------------
NM_001130112.2      CGAGAAGATCCAGCTGAGCTCCCTCATTGCTGCATTTCAAGTCACCAGAGACCTGATTGT
NM_007370.5         CGAGAAGATCCAGCTGAGCTCCCTCATTGCTGCATTTCAAGTCACCAGAGACCTGATTGT
NM_181578.3         CGAGAAGATCCAGCTGAGCTCCCTCATTGCTGCATTTCAAGTCACCAGAGACCTGATTGT
NM_001206801.1      CCAGCTGAGCTCCCTCATTGCTGCATTTCAAGTCACCAGAGACCTGATTGTTGCAGAGGC
                                                                                

HsCD00583837        -------------
NM_001130112.2      TGCAGAGGCCTAG
NM_007370.5         TGCAGAGGCCTAG
NM_181578.3         TGCAGAGGCCTAG
NM_001206801.1      CTAG---------

Pathways

NCI :

ATR signaling pathway

NCI :

Fanconi anemia pathway

Panther :

DNA replication

Reactome :

Activation of ATR in response to replication stress

Reactome :

Cell Cycle

Reactome :

Cell Cycle Checkpoints

Reactome :

Cell Cycle, Mitotic

Reactome :

Chromosome Maintenance

Reactome :

DNA Repair

Reactome :

DNA Replication

Reactome :

DNA strand elongation

Reactome :

Extension of Telomeres

Reactome :

G2/M Checkpoints

Reactome :

Gap-filling DNA repair synthesis and ligation in GG-NER

Reactome :

Gap-filling DNA repair synthesis and ligation in TC-NER

Reactome :

Global Genomic NER (GG-NER)

Reactome :

Lagging Strand Synthesis

Reactome :

Leading Strand Synthesis

Reactome :

Nucleotide Excision Repair

Reactome :

Polymerase switching

Reactome :

Polymerase switching on the C-strand of the telomere

Reactome :

Repair synthesis for gap-filling by DNA polymerase in TC-NER

Reactome :

Repair synthesis of patch ~27-30 bases long by DNA polymerase

Reactome :

S Phase

Reactome :

Synthesis of DNA

Reactome :

Telomere C-strand (Lagging Strand) Synthesis

Reactome :

Telomere Maintenance

Reactome :

Transcription-coupled NER (TC-NER)

WikiPathway :

DNA Replication

Protein

SMART domain :

AAA : ATPases associated with a variety of cellular activities

Structural Annotation Wiki :

HR3045B

UniProt :

P40937

HPRD :

02677

Clone

Cloning Information :

627027

Original Clone ID :

HR3045B-15-257-15.3

Experimental

TargetTrack - Experimental data :

HR3045B

Organism

Species Specific ID:

5985

Genome

Chromosome :

Map Location :

12q24.23

Ensembl :

ENSG00000111445

Reagents

Labome :

RFC5-antibody

RFC5 (H. sapiens )In pET15_NESG (His-tagged bacterial expression vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Reference Sequence Alignment

Pathways

Protein

Clone

Experimental

Organism

Genome

Reagents

RFC5 (H. sapiens )
In pET15_NESG (His-tagged bacterial expression vector)