DNASU Plasmid | PSMC5 (H. sapiens )<br>In pET15_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: PSMC5

Gene Name: proteasome (prosome, macropain) 26S subunit, ATPase, 5

Original Clone ID:

HR3102B-36-393-15.4

Keyword:

full length protein truncation

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pET15_NESG

Format:

CLOSED

Source:

Northeast Structural Genomics Consortium

Description:

None

Comments:

None

Mutation / Discrepancy:

No / Yes

Publications:

None

Authors:

PSI:Biology
Northeast Structural Genomics Consortium

HsCD00583906

Price:

AVAILABLE

No restriction
Special MTA: PSI/NESG

Insert Sequence
Protein Sequence

Insert sequence: 1023nts

Open reading frame: 51 to 1073

Reference Sequence Alignment

TTTAAGAAGGAGATATACCATGGGGCCATCACCATCACCATCACAGCCATATGAACGATA
AGAGCCAAAACCTCCGGAGGCTGCAGGCACAGAGGAACGAACTAAATGCTAAAGTTCGCC
TATTGCGGGAGGAGCTACAGCTGCTGCAGGAGCAGGGCTCCTATGTGGGGGAAGTAGTCC
GGGCCATGGATAAGAAGAAAGTGTTGGTCAAGGTGACACCCAATTGCCGGGTGGCTCTAA
GGAATGACAGCTACACTCTGCACAAGATCCTGCCCAACAAGGTAGACCCATTAGTGTCAC
TGATGATGGTGGAGAAAGTACCAGATTCAACTTATGAGATGATTGGTGGACTGGACAAAC
AGATCAAGGAGATCAAAGAAGTGATCGAGCTGCCTGTTAAGCATCCTGAGCTCTTCGAAG
CACTGGGCATTGCTCAGCCCAAGGGAGTGCTGCTGTATGGACCTCCAGGCACTGGGAAGA
CACTGTTGGCCCGGGCTGTGGCTCATCATACGGACTGTACCTTTATTCGTGTCTCTGGCT
CTGAATTGGTACAGAAATTCATAGGGGAAGGGGCAAGAATGGTGAGGGAGCTGTTTGTCA
TGGCACGGGAACATGCTCCATCTATCATCTTCATGGACGAAATCGACTCCATCGGCTCCT
CGCGGCTGGAGGGGGGTTCTGGAGGGGACAGTGAAGTGCAGCGCACGATGCTGGAGTTGC
TCAACCAGCTTGACGGCTTTGAGGCCACCAAGAACATCAAGGTTATCATGGCTACTAATA
GGATTGATATCCTGGACTCGGCACTGCTTCGCCCAGGGCGCATTGACAGAAAAATTGAAT
TCCCACCCCCCAATGAGGAGGCCCGGCTGGACATTTTGAAGATTCATTCTCGGAAGATGA
ACCTGACCCGGGGGATCAACCTGAGAAAAATTGCTGAGCTCATGCCAGGAGCATCAGGGG
CTGAAGTGAAGGGCGTGTGCACAGAAGCTGGCATGTATGCCCTGCGAGAACGGCGAGTCC
ATGTCACTCAGGAGGACTTTGAGATGGCAGTAGCCAAGGTCATGCAGAAGTAG

Protein sequence predicted based on nucleotide sequence

MNDKSQNLRRLQAQRNELNAKVRLLREELQLLQEQGSYVGEVVRAMDKKKVLVKVTPNCR
VALRNDSYTLHKILPNKVDPLVSLMMVEKVPDSTYEMIGGLDKQIKEIKEVIELPVKHPE
LFEALGIAQPKGVLLYGPPGTGKTLLARAVAHHTDCTFIRVSGSELVQKFIGEGARMVRE
LFVMAREHAPSIIFMDEIDSIGSSRLEGGSGGDSEVQRTMLELLNQLDGFEATKNIKVIM
ATNRIDILDSALLRPGRIDRKIEFPPPNEEARLDILKIHSRKMNLTRGINLRKIAELMPG
ASGAEVKGVCTEAGMYALRERRVHVTQEDFEMAVAKVMQK*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

t6c,N2N;del@163,57;

c553t,L185L;c738t,L246L

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinPurified: Not_Tested

ProteinExpressed: Protein_Confirmed

SolubleProtein: Tested_Not_Soluble

Find experimental protocols in HR3102B

Recommended expression in:

Not Applicable

Vector Name:

pET15_NESG

Synonyms:

pET15, pET15-modified, pET15Cc, pET15_NESG

Sequencing Primer:

Forward: T7

Reverse: T7 terminator

Description:

Bacterial (E. coli) expression vector with T7 lac promoter, adds N-terminal 6x His tag; amp resistance; restriction enzyme cloning.

Comments:

Modified Novagen pET vector-common MCS

Size (bp):

5723

Parent Vector:

None

Properties:

bacterial expression, in vitro transcription, multiple cloning site, with tag/fusion/marker

Author Name:

Gaetano T. Montleione
Jessica Bearden
Northeast Structural Genomics Consortium

Publications:

PMID: 15808222

Title: Robotic cloning and Protein Production Platform of the Northeast Structural Genomics Consortium

Vector Map:

Vector Sequence:

Sequence:

TTCTCATGTTTGACAGCTTATCATCGATAAGCTAGCTTTAATGCGGTAGTTTATCACAGTTAAATTGCTAACGCAGTCAGGCACCGTGTATGAAATCTAACAATGCGCTCATCGTCATCCTCGGCACCGTCACCCTGGATGCTGTAGGCATAGGCTTGGTTATGCCGGTACTGCCGGGCCTCTTGCGGGATATCCGGATATAGTTCCTCCTTTCAGCAAAAAACCCCTCAAGACCCGTTTAGAGGCCCCAAGGGGTTATGCTAGTTATTGCTCAGCGGTGGCAGCAGCCAACTCAGCTTCCTTTCGGGCTTTGTTAGCAGCCGGATCTCGAGAAGCTTTGCGTCGACAGAGCTCGCAGGATCCGCAGAATTCGCCATATGGCTGTGATGGTGATGGTGATGGCCCATGGTATATCTCCTTCTTAAAGTTAAACAAAATTATTTCTAGAGGGGAATTGTTATCCGCTCACAATTCCCCTATAGTGAGTCGTATTAATTTCGCGGGATCGAGATCTCGATCCTCTACGCCGGACGCATCGTGGCCGGCATCACCGGCGCCACAGGTGCGGTTGCTGGCGCCTATATCGCCGACATCACCGATGGGGAAGATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTGTTTCGGCGTGGGTATGGTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCTTGCATGCACCATTCCTTGCGGCGGCGGTGCTCAACGGCCTCAACCTACTACTGGGCTGCTTCCTAATGCAGGAGTCGCATAAGGGAGAGCGTCGAGATCCCGGACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAATGTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAATGCTGAATGAGGGCATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTGCGGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTTATATCCCGCCGTTAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTAAGTTAGCTCACTCATTAGGCACCGGGATCTCGACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACTATCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGCGCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCCTTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTCCCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTGTCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACCAGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGCCTCGGCGAGCACATGGAACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGTCGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACGGATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAACCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCATCTCGGGCAGCGTTGGGTCCTGGCCACGGGTGCGCATGATCGTGCTCCTGTCGTTGAGGACCCGGCTAGGCTGGCGGGGTTGCCTTACTGGTTAGCAGAATGAATCACCGATACGCGAGCGAACGTGAAGCGACTGCTGCTGCAAAACGTCTGCGACCTGAGCAACAACATGAATGGTCTTCGGTTTCCGTGTTTCGTAAAGTCTGGAAACGCGGAAGTCAGCGCCCTGCACCATTATGTTCCGGATCTGCATCGCAGGATGCTGCTGGCTACCCTGTGGAACACCTACATCTGTATTAACGAAGCGCTGGCATTGACCCTGAGTGATTTTTCTCTGGTCCCGCCGCATCCATACCGCCAGTTGTTTACCCTCACAACGTTCCAGTAACCGGGCATGTTCATCATCAGTAACCCGTATCGTGAGCATCCTCTCTCGTTTCATCGGTATCATTACCCCCATGAACAGAAATCCCCCTTACACGGAGGCATCAGTGACCAAACAGGAAAAAACCGCCCTTAACATGGCCCGCTTTATCAGAAGCCAGACATTAACGCTTCTGGAGAAACTCAACGAGCTGGACGCGGATGAACAGGCAGACATCTGTGAATCGCTTCACGACCACGCTGATGAGCTTTACCGCAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTGCAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAACACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTTCAAGAA

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (includes SphI, XbaI, EcoRI, BamHI, XhoI)

327

452

bacterial origin

ori

ColE1 origin of replication

3881

4563

gene fragment

ROP

repressor of primer ROP sequence

3281

3457

primer

T7 forward

T7 forward sequencing primer

481

496

primer

T7 reverse

T7 reverse sequencing primer

257

275

promoter

T7 promoter

478

497

repressor binding site

lacO

lacO regulatory sequence

456

478

selectable marker

AmpR

ampicillin resistance gene

4661

5230

tag

His

N-terminal 6x His tag

387

404

trxn termination sequence

T7 term

T7 termination sequence

257

275

Gene

Gene Symbol :

PSMC5

Symbol Nomenclature :

PSMC5

SYNONYM :

S8; SUG-1; SUG1; TBP10; TRIP1; p45; p45/SUG

Designation :

Full Nomenclature :

proteasome (prosome, macropain) 26S subunit, ATPase, 5

GENEID :

5705

GI :

24497435

GenBank Accession :

NM_002805

HGNC :

9552

MIM :

601681

Vega :

OTTHUMG00000179013

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00583906        ----------------------------------------------------TTTAAGAA
NM_001199163.1      ------------------------ATGGAGCTGGAGGAGGGGAAGGCAGGCAGCGGACTC
NM_002805.5         ATGGCGCTTGACGGACCAGAGCAGATGGAGCTGGAGGAGGGGAAGGCAGGCAGCGGACTC
                                                                           .* :.

HsCD00583906        GGAGATATACCATGGGGCCATCACCATCACCATCACAGCCATATGAACGATAAGAGCCAA
NM_001199163.1      CGCCAATATTATCTGTCCAAGATTGAAGAACTCCAGCTGATTGTGAATGATAAGAGCCAA
NM_002805.5         CGCCAATATTATCTGTCCAAGATTGAAGAACTCCAGCTGATTGTGAATGATAAGAGCCAA
                     *. *:::: .:  *  *.* .:  *: *.*: ** .  .:*.**** ************

HsCD00583906        AACCTCCGGAGGCTGCAGGCACAGAGGAACGAACTAAATGCTAAAGTTCGCCTATTGCGG
NM_001199163.1      AACCTCCGGAGGCTGCAGGCACAGAGGAACGAACTAAATGCTAAAGTTCGCCTATTGCGG
NM_002805.5         AACCTCCGGAGGCTGCAGGCACAGAGGAACGAACTAAATGCTAAAGTTCGCCTATTGCGG
                    ************************************************************

HsCD00583906        GAGGAGCTACAGCTGCTGCAGGAGCAGGGCTCCTATGTGGGGGAAGTAGTCCGGGCCATG
NM_001199163.1      GAGGAGCTACAGCTGCTGCAGGAGCAGGGCTCCTATGTGGGGGAAGTAGTCCGGGCCATG
NM_002805.5         GAGGAGCTACAGCTGCTGCAGGAGCAGGGCTCCTATGTGGGGGAAGTAGTCCGGGCCATG
                    ************************************************************

HsCD00583906        GATAAGAAGAAAGTGTTGGTCAAG------------------------------------
NM_001199163.1      GATAAGAAGAAAGTGTTGGTCAAGGTACATCCTGAAGGTAAATTTGTTGTAGACGTGGAC
NM_002805.5         GATAAGAAGAAAGTGTTGGTCAAGGTACATCCTGAAGGTAAATTTGTTGTAGACGTGGAC
                    ************************                                    

HsCD00583906        ---------------------GTGACACCCAATTGCCGGGTGGCTCTAAGGAATGACAGC
NM_001199163.1      AAAAACATTGACATCAATGATGTGACACCCAATTGCCGGGTGGCTCTAAGGAATGACAGC
NM_002805.5         AAAAACATTGACATCAATGATGTGACACCCAATTGCCGGGTGGCTCTAAGGAATGACAGC
                                         ***************************************

HsCD00583906        TACACTCTGCACAAGATCCTGCCCAACAAGGTAGACCCATTAGTGTCACTGATGATGGTG
NM_001199163.1      TACACTCTGCACAAGATCCTGCCCAACAAGGTAGACCCATTAGTGTCACTGATGATGGTG
NM_002805.5         TACACTCTGCACAAGATCCTGCCCAACAAGGTAGACCCATTAGTGTCACTGATGATGGTG
                    ************************************************************

HsCD00583906        GAGAAAGTACCAGATTCAACTTATGAGATGATTGGTGGACTGGACAAACAGATCAAGGAG
NM_001199163.1      GAGAAAGTACCAGATTCAACTTATGAGATGATTGGTGGACTGGACAAACAGATCAAGGAG
NM_002805.5         GAGAAAGTACCAGATTCAACTTATGAGATGATTGGTGGACTGGACAAACAGATCAAGGAG
                    ************************************************************

HsCD00583906        ATCAAAGAAGTGATCGAGCTGCCTGTTAAGCATCCTGAGCTCTTCGAAGCACTGGGCATT
NM_001199163.1      ATCAAAGAAGTGATCGAGCTGCCTGTTAAGCATCCTGAGCTCTTCGAAGCACTGGGCATT
NM_002805.5         ATCAAAGAAGTGATCGAGCTGCCTGTTAAGCATCCTGAGCTCTTCGAAGCACTGGGCATT
                    ************************************************************

HsCD00583906        GCTCAGCCCAAGGGAGTGCTGCTGTATGGACCTCCAGGCACTGGGAAGACACTGTTGGCC
NM_001199163.1      GCTCAGCCCAAGGGAGTGCTGCTGTATGGACCTCCAGGCACTGGGAAGACACTGTTGGCC
NM_002805.5         GCTCAGCCCAAGGGAGTGCTGCTGTATGGACCTCCAGGCACTGGGAAGACACTGTTGGCC
                    ************************************************************

HsCD00583906        CGGGCTGTGGCTCATCATACGGACTGTACCTTTATTCGTGTCTCTGGCTCTGAATTGGTA
NM_001199163.1      CGGGCTGTGGCTCATCATACGGACTGTACCTTTATTCGTGTCTCTGGCTCTGAACTGGTA
NM_002805.5         CGGGCTGTGGCTCATCATACGGACTGTACCTTTATTCGTGTCTCTGGCTCTGAACTGGTA
                    ****************************************************** *****

HsCD00583906        CAGAAATTCATAGGGGAAGGGGCAAGAATGGTGAGGGAGCTGTTTGTCATGGCACGGGAA
NM_001199163.1      CAGAAATTCATAGGGGAAGGGGCAAGAATGGTGAGGGAGCTGTTTGTCATGGCACGGGAA
NM_002805.5         CAGAAATTCATAGGGGAAGGGGCAAGAATGGTGAGGGAGCTGTTTGTCATGGCACGGGAA
                    ************************************************************

HsCD00583906        CATGCTCCATCTATCATCTTCATGGACGAAATCGACTCCATCGGCTCCTCGCGGCTGGAG
NM_001199163.1      CATGCTCCATCTATCATCTTCATGGACGAAATCGACTCCATCGGCTCCTCGCGGCTGGAG
NM_002805.5         CATGCTCCATCTATCATCTTCATGGACGAAATCGACTCCATCGGCTCCTCGCGGCTGGAG
                    ************************************************************

HsCD00583906        GGGGGTTCTGGAGGGGACAGTGAAGTGCAGCGCACGATGCTGGAGTTGCTCAACCAGCTT
NM_001199163.1      GGGGGTTCTGGAGGGGACAGTGAAGTGCAGCGCACGATGCTGGAGTTGCTCAACCAGCTC
NM_002805.5         GGGGGTTCTGGAGGGGACAGTGAAGTGCAGCGCACGATGCTGGAGTTGCTCAACCAGCTC
                    *********************************************************** 

HsCD00583906        GACGGCTTTGAGGCCACCAAGAACATCAAGGTTATCATGGCTACTAATAGGATTGATATC
NM_001199163.1      GACGGCTTTGAGGCCACCAAGAACATCAAGGTTATCATGGCTACTAATAGGATTGATATC
NM_002805.5         GACGGCTTTGAGGCCACCAAGAACATCAAGGTTATCATGGCTACTAATAGGATTGATATC
                    ************************************************************

HsCD00583906        CTGGACTCGGCACTGCTTCGCCCAGGGCGCATTGACAGAAAAATTGAATTCCCACCCCCC
NM_001199163.1      CTGGACTCGGCACTGCTTCGCCCAGGGCGCATTGACAGAAAAATTGAATTCCCACCCCCC
NM_002805.5         CTGGACTCGGCACTGCTTCGCCCAGGGCGCATTGACAGAAAAATTGAATTCCCACCCCCC
                    ************************************************************

HsCD00583906        AATGAGGAGGCCCGGCTGGACATTTTGAAGATTCATTCTCGGAAGATGAACCTGACCCGG
NM_001199163.1      AATGAGGAGGCCCGGCTGGACATTTTGAAGATTCATTCTCGGAAGATGAACCTGACCCGG
NM_002805.5         AATGAGGAGGCCCGGCTGGACATTTTGAAGATTCATTCTCGGAAGATGAACCTGACCCGG
                    ************************************************************

HsCD00583906        GGGATCAACCTGAGAAAAATTGCTGAGCTCATGCCAGGAGCATCAGGGGCTGAAGTGAAG
NM_001199163.1      GGGATCAACCTGAGAAAAATTGCTGAGCTCATGCCAGGAGCATCAGGGGCTGAAGTGAAG
NM_002805.5         GGGATCAACCTGAGAAAAATTGCTGAGCTCATGCCAGGAGCATCAGGGGCTGAAGTGAAG
                    ************************************************************

HsCD00583906        GGCGTGTGCACAGAAGCTGGCATGTATGCCCTGCGAGAACGGCGAGTCCATGTCACTCAG
NM_001199163.1      GGCGTGTGCACAGAAGCTGGCATGTATGCCCTGCGAGAACGGCGAGTCCATGTCACTCAG
NM_002805.5         GGCGTGTGCACAGAAGCTGGCATGTATGCCCTGCGAGAACGGCGAGTCCATGTCACTCAG
                    ************************************************************

HsCD00583906        GAGGACTTTGAGATGGCAGTAGCCAAGGTCATGCAGAAGTAG------------------
NM_001199163.1      GAGGACTTTGAGATGGCAGTAGCCAAGGTCATGCAGAAGGACAGTGAGAAAAACATGTCC
NM_002805.5         GAGGACTTTGAGATGGCAGTAGCCAAGGTCATGCAGAAGGACAGTGAGAAAAACATGTCC
                    *************************************** *                   

HsCD00583906        ---------------------
NM_001199163.1      ATCAAGAAATTATGGAAGTGA
NM_002805.5         ATCAAGAAATTATGGAAGTGA

Pathways

Reactome :

Activation of APC/C and APC/C:Cdc20 mediated degradation of mitotic proteins

Reactome :

Activation of NF-kappaB in B cells

Reactome :

Adaptive Immune System

Reactome :

Antigen processing-Cross presentation

Reactome :

Antigen processing: Ubiquitination & Proteasome degradation

Reactome :

APC/C-mediated degradation of cell cycle proteins

Reactome :

APC/C:Cdc20 mediated degradation of mitotic proteins

Reactome :

APC/C:Cdc20 mediated degradation of Securin

Reactome :

APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1

Reactome :

Apoptosis

Reactome :

Assembly of the pre-replicative complex

Reactome :

Asymmetric localization of PCP proteins

Reactome :

AUF1 (hnRNP D0) destabilizes mRNA

Reactome :

Autodegradation of Cdh1 by Cdh1:APC/C

Reactome :

Autodegradation of the E3 ubiquitin ligase COP1

Reactome :

beta-catenin independent WNT signaling

Reactome :

Cdc20:Phospho-APC/C mediated degradation of Cyclin A

Reactome :

CDK-mediated phosphorylation and removal of Cdc6

Reactome :

CDT1 association with the CDC6:ORC:origin complex

Reactome :

Cell Cycle

Reactome :

Cell Cycle Checkpoints

Reactome :

Cell Cycle, Mitotic

Reactome :

Class I MHC mediated antigen processing & presentation

Reactome :

Cross-presentation of soluble exogenous antigens (endosomes)

Reactome :

Cyclin A:Cdk2-associated events at S phase entry

Reactome :

Cyclin E associated events during G1/S transition

Reactome :

degradation of AXIN

Reactome :

degradation of DVL

Reactome :

Disease

Reactome :

DNA Replication

Reactome :

DNA Replication Pre-Initiation

Reactome :

Downstream signaling events of B Cell Receptor (BCR)

Reactome :

ER-Phagosome pathway

Reactome :

G1/S DNA Damage Checkpoints

Reactome :

G1/S Transition

Reactome :

Gene Expression

Reactome :

HIV Infection

Reactome :

Host Interactions of HIV factors

Reactome :

Immune System

Reactome :

M Phase

Reactome :

M/G1 Transition

Reactome :

Metabolism

Reactome :

Metabolism of amino acids and derivatives

Reactome :

Metabolism of mRNA

Reactome :

Metabolism of RNA

Reactome :

Mitotic Anaphase

Reactome :

Mitotic G1-G1/S phases

Reactome :

Mitotic M-M/G1 phases

Reactome :

Mitotic Metaphase and Anaphase

Reactome :

Orc1 removal from chromatin

Reactome :

p53-Dependent G1 DNA Damage Response

Reactome :

p53-Dependent G1/S DNA damage checkpoint

Reactome :

p53-Independent DNA Damage Response

Reactome :

p53-Independent G1/S DNA damage checkpoint

Reactome :

PCP/CE pathway

Reactome :

Regulation of activated PAK-2p34 by proteasome mediated degradation

Reactome :

Regulation of APC/C activators between G1/S and early anaphase

Reactome :

Regulation of Apoptosis

Reactome :

Regulation of DNA replication

Reactome :

Regulation of mitotic cell cycle

Reactome :

Regulation of mRNA stability by proteins that bind AU-rich elements

Reactome :

Regulation of ornithine decarboxylase (ODC)

Reactome :

Removal of licensing factors from origins

Reactome :

S Phase

Reactome :

SCF(Skp2)-mediated degradation of p27/p21

Reactome :

SCF-beta-TrCP mediated degradation of Emi1

Reactome :

Separation of Sister Chromatids

Reactome :

Signal Transduction

Reactome :

Signaling by the B Cell Receptor (BCR)

Reactome :

Signaling by Wnt

Reactome :

Stabilization of p53

Reactome :

Switching of origins to a post-replicative state

Reactome :

Synthesis of DNA

Reactome :

TCF dependent signaling in response to WNT

Reactome :

Ubiquitin Mediated Degradation of Phosphorylated Cdc25A

Reactome :

Ubiquitin-dependent degradation of Cyclin D

Reactome :

Ubiquitin-dependent degradation of Cyclin D1

Reactome :

Vif-mediated degradation of APOBEC3G

Reactome :

Vpu mediated degradation of CD4

WikiPathway :

Proteasome Degradation

Protein

SMART domain :

AAA : ATPases associated with a variety of cellular activities

Structural Annotation Wiki :

HR3102B

UniProt :

P62195

HPRD :

03400

Clone

Cloning Information :

626589

Original Clone ID :

HR3102B-36-393-15.4

Experimental

TargetTrack - Experimental data :

HR3102B

Organism

Species Specific ID:

5705

Genome

Chromosome :

Map Location :

17q23.3

Ensembl :

ENSG00000087191

Reagents

Labome :

PSMC5-antibody

PSMC5 (H. sapiens )In pET15_NESG (His-tagged bacterial expression vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Reference Sequence Alignment

Pathways

Protein

Clone

Experimental

Organism

Genome

Reagents

PSMC5 (H. sapiens )
In pET15_NESG (His-tagged bacterial expression vector)