DNASU Plasmid | RPAP3 (H. sapiens )<br>In pET15_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: RPAP3

Gene Name: RNA polymerase II associated protein 3

Original Clone ID:

HR9155-1-460-15.3

Keyword:

targeted domain

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pET15_NESG

Format:

CLOSED

Source:

Northeast Structural Genomics Consortium

Description:

None

Comments:

None

Mutation / Discrepancy:

No / Yes

Publications:

None

Authors:

PSI:Biology
Northeast Structural Genomics Consortium

HsCD00583938

Price:

AVAILABLE

No restriction
Special MTA: PSI/NESG

Insert Sequence
Protein Sequence

Insert sequence: 1383nts

Open reading frame: 26 to 1408

Reference Sequence Alignment

CCATCACCATCACCATCACAGCCATATGACTTCAGCAAATAAAGCAATCGAATTACAACT
ACAAGTGAAACAAAATGCAGAAGAATTACAAGACTTTATGCGGGATTTAGAGAACTGGGA
AAAAGACATTAAACAAAAGGATATGGAACTAAGAAGACAGAATGGTGTTCCTGAAGAGAA
TTTACCTCCTATTCGAAATGGGAATTTTAGGAAAAAGAAGAAAGGCAAAGCTAAAGAGTC
TTCCAAAAAAACCAGAGAGGAAAACACAAAAAACAGGATAAAATCTTATGATTATGAGGC
ATGGGCAAAGCTTGATGTGGACCGTATCCTTGATGAGCTTGACAAAGACGATAGTACCCA
TGAGTCTCTGTCTCAAGAATCAGAGTCGGAGGAAGATGGGATTCATGTAGATTCACAAAA
GGCTCTTGTTTTAAAAGAAAAGGGCAATAAATACTTCAAACAAGGAAAATATGATGAAGC
AATTGACTGCTACACAAAAGGCATGGATGCCGATCCATATAATCCCGTGTTGCCAACGAA
CAGAGCGTCAGCATATTTTAGACTGAAAAAATTTGCTGTTGCTGAGTCTGATTGTAATTT
AGCAGTTGCCTTGAATAGAAGTTATACAAAGGCTTATTCCAGACGAGGTGCTGCTCGATT
TGCTTTGCAAAAATTAGAAGAGGCCAAAAAAGATTATGAAAGAGTATTAGAACTAGAACC
AAATAACTTTGAAGCAACAAATGAACTCAGGAAAATCAGTCAGGCTTTAGCATCCAAAGA
AAACTCATATCCAAAGGAAGCTGACATAGCGATTAAGTCAACAGAAGGAGAGCGAAAGCA
AATTGAAGCACAACAGAATAAGCAGCAGGCCATTTCAGAGAAAGATCGGGGGAATGGATT
TTTCAAAGAGGGGAAATATGAAAGAGCAATTGAATGCTATACTCGAGGGATAGCAGCAGA
TGGTGCTAATGCCCTTCTTCCAGCTAACAGAGCTATGGCCTATCTGAGGATTCAGAAATA
TGAAGAAGCTGAAAAAGACTGCACACAAGCCATTTTATTAGATGGCTCATATTCTAAAGC
TTTTGCCGGAAGAGGAACTGCAAGAACATTTTTGGGAAAGCTAAATGAGGCAAAACAAGA
TTTTGAAACTGTTTTACTTCTGGAACCTGGAAATAAGCAAGCAGCAACTGAACTCTCCAA
AATTAAAAAGGAATTAATTGAGAAAGGACGCTGGGATGATGTCTTTCTTGATTCCACACA
AAGACAAAATGTGGTAAAACCCATTGATAATCCACCGCATCCTGGATCAACTAAACCACT
CAAGAAGGTTATTATTGAAGAAACTGGTAATTTGATGCAGACTATTGATGTGCCAGATAG
CACTACTGCTGCTGCTCCAGAGAATTAG

Protein sequence predicted based on nucleotide sequence

MTSANKAIELQLQVKQNAEELQDFMRDLENWEKDIKQKDMELRRQNGVPEENLPPIRNGN
FRKKKKGKAKESSKKTREENTKNRIKSYDYEAWAKLDVDRILDELDKDDSTHESLSQESE
SEEDGIHVDSQKALVLKEKGNKYFKQGKYDEAIDCYTKGMDADPYNPVLPTNRASAYFRL
KKFAVAESDCNLAVALNRSYTKAYSRRGAARFALQKLEEAKKDYERVLELEPNNFEATNE
LRKISQALASKENSYPKEADIAIKSTEGERKQIEAQQNKQQAISEKDRGNGFFKEGKYER
AIECYTRGIAADGANALLPANRAMAYLRIQKYEEAEKDCTQAILLDGSYSKAFAGRGTAR
TFLGKLNEAKQDFETVLLLEPGNKQAATELSKIKKELIEKGRWDDVFLDSTQRQNVVKPI
DNPPHPGSTKPLKKVIIEETGNLMQTIDVPDSTTAAAPEN*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

a87g,E29E;a285g,K95K;

a366g,E122E;t785c,V262A;

a983g,K328R;a1063g,R355G;

t1160c,V387A;a1205g,H402R;

a1332g,I444M

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinPurified: Not_Tested

ProteinExpressed: Protein_Confirmed

SolubleProtein: Protein_Soluble

Find experimental protocols in HR9155

Recommended expression in:

Not Applicable

Vector Name:

pET15_NESG

Synonyms:

pET15, pET15-modified, pET15Cc, pET15_NESG

Sequencing Primer:

Forward: T7

Reverse: T7 terminator

Description:

Bacterial (E. coli) expression vector with T7 lac promoter, adds N-terminal 6x His tag; amp resistance; restriction enzyme cloning.

Comments:

Modified Novagen pET vector-common MCS

Size (bp):

5723

Parent Vector:

None

Properties:

bacterial expression, in vitro transcription, multiple cloning site, with tag/fusion/marker

Author Name:

Gaetano T. Montleione
Jessica Bearden
Northeast Structural Genomics Consortium

Publications:

PMID: 15808222

Title: Robotic cloning and Protein Production Platform of the Northeast Structural Genomics Consortium

Vector Map:

Vector Sequence:

Sequence:

TTCTCATGTTTGACAGCTTATCATCGATAAGCTAGCTTTAATGCGGTAGTTTATCACAGTTAAATTGCTAACGCAGTCAGGCACCGTGTATGAAATCTAACAATGCGCTCATCGTCATCCTCGGCACCGTCACCCTGGATGCTGTAGGCATAGGCTTGGTTATGCCGGTACTGCCGGGCCTCTTGCGGGATATCCGGATATAGTTCCTCCTTTCAGCAAAAAACCCCTCAAGACCCGTTTAGAGGCCCCAAGGGGTTATGCTAGTTATTGCTCAGCGGTGGCAGCAGCCAACTCAGCTTCCTTTCGGGCTTTGTTAGCAGCCGGATCTCGAGAAGCTTTGCGTCGACAGAGCTCGCAGGATCCGCAGAATTCGCCATATGGCTGTGATGGTGATGGTGATGGCCCATGGTATATCTCCTTCTTAAAGTTAAACAAAATTATTTCTAGAGGGGAATTGTTATCCGCTCACAATTCCCCTATAGTGAGTCGTATTAATTTCGCGGGATCGAGATCTCGATCCTCTACGCCGGACGCATCGTGGCCGGCATCACCGGCGCCACAGGTGCGGTTGCTGGCGCCTATATCGCCGACATCACCGATGGGGAAGATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTGTTTCGGCGTGGGTATGGTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCTTGCATGCACCATTCCTTGCGGCGGCGGTGCTCAACGGCCTCAACCTACTACTGGGCTGCTTCCTAATGCAGGAGTCGCATAAGGGAGAGCGTCGAGATCCCGGACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAATGTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAATGCTGAATGAGGGCATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTGCGGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTTATATCCCGCCGTTAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTAAGTTAGCTCACTCATTAGGCACCGGGATCTCGACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACTATCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGCGCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCCTTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTCCCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTGTCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACCAGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGCCTCGGCGAGCACATGGAACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGTCGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACGGATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAACCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCATCTCGGGCAGCGTTGGGTCCTGGCCACGGGTGCGCATGATCGTGCTCCTGTCGTTGAGGACCCGGCTAGGCTGGCGGGGTTGCCTTACTGGTTAGCAGAATGAATCACCGATACGCGAGCGAACGTGAAGCGACTGCTGCTGCAAAACGTCTGCGACCTGAGCAACAACATGAATGGTCTTCGGTTTCCGTGTTTCGTAAAGTCTGGAAACGCGGAAGTCAGCGCCCTGCACCATTATGTTCCGGATCTGCATCGCAGGATGCTGCTGGCTACCCTGTGGAACACCTACATCTGTATTAACGAAGCGCTGGCATTGACCCTGAGTGATTTTTCTCTGGTCCCGCCGCATCCATACCGCCAGTTGTTTACCCTCACAACGTTCCAGTAACCGGGCATGTTCATCATCAGTAACCCGTATCGTGAGCATCCTCTCTCGTTTCATCGGTATCATTACCCCCATGAACAGAAATCCCCCTTACACGGAGGCATCAGTGACCAAACAGGAAAAAACCGCCCTTAACATGGCCCGCTTTATCAGAAGCCAGACATTAACGCTTCTGGAGAAACTCAACGAGCTGGACGCGGATGAACAGGCAGACATCTGTGAATCGCTTCACGACCACGCTGATGAGCTTTACCGCAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTGCAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAACACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTTCAAGAA

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (includes SphI, XbaI, EcoRI, BamHI, XhoI)

327

452

bacterial origin

ori

ColE1 origin of replication

3881

4563

gene fragment

ROP

repressor of primer ROP sequence

3281

3457

primer

T7 forward

T7 forward sequencing primer

481

496

primer

T7 reverse

T7 reverse sequencing primer

257

275

promoter

T7 promoter

478

497

repressor binding site

lacO

lacO regulatory sequence

456

478

selectable marker

AmpR

ampicillin resistance gene

4661

5230

tag

His

N-terminal 6x His tag

387

404

trxn termination sequence

T7 term

T7 termination sequence

257

275

Gene

Gene Symbol :

RPAP3

Symbol Nomenclature :

RPAP3

Designation :

RNA polymerase II-associated protein 3

Full Nomenclature :

RNA polymerase II associated protein 3

GENEID :

79657

GI :

225735591

GenBank Accession :

NM_024604

HGNC :

26151

MIM :

611477

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00583938        ------------------------------------------------------------
NM_024604.2         ATGACTTCAGCAAATAAAGCAATCGAATTACAACTACAAGTGAAACAAAATGCAGAAGAA
NM_001146075.1      ATGACTTCAGCAAATAAAGCAATCGAATTACAACTACAAGTGAAACAAAATGCAGAAGAA
NM_001146076.1      ------------------------------------------------------------
                                                                                

HsCD00583938        ------------------------------------------------------------
NM_024604.2         TTACAAGACTTTATGCGGGATTTAGAAAACTGGGAAAAAGACATTAAACAAAAGGATATG
NM_001146075.1      TTACAAGACTTTATGCGGGATTTAGAAAACTGGGAAAAAGACATTAAACAAAAGGATATG
NM_001146076.1      ------------------------------------------------------------
                                                                                

HsCD00583938        ------------------------------------------------------------
NM_024604.2         GAACTAAGAAGACAGAATGGTGTTCCTGAAGAGAATTTACCTCCTATTCGAAATGGGAAT
NM_001146075.1      GAACTAAGAAGACAGAATGGTGTTCCTGAAGAGAATTTACCTCCTATTCGAAATGGGAAT
NM_001146076.1      ------------------------------------------------------------
                                                                                

HsCD00583938        ------------------------------------------------------------
NM_024604.2         TTTAGGAAAAAGAAGAAAGGCAAAGCTAAAGAGTCTTCCAAAAAAACCAGAGAGGAAAAC
NM_001146075.1      TTTAGGAAAAAGAAGAAAGGCAAAGCTAAAGAGTCTTCCAAAAAAACCAGAGAGGAAAAC
NM_001146076.1      ------------------------------------------------------------
                                                                                

HsCD00583938        ------------------------------------------------------------
NM_024604.2         ACAAAAAACAGGATAAAATCTTATGATTATGAGGCATGGGCAAAACTTGATGTGGACCGT
NM_001146075.1      ACAAAAAACAGGATAAAATCTTATGATTATGAGGCATGGGCAAAACTTGATGTGGACCGT
NM_001146076.1      ------------------------------------------------------------
                                                                                

HsCD00583938        ------------------------------------------------------------
NM_024604.2         ATCCTTGATGAGCTTGACAAAGACGATAGTACCCATGAGTCTCTGTCTCAAGAATCAGAG
NM_001146075.1      ATCCTTGATGAGCTTGACAAAGACGATAGTACCCATGAGTCTCTGTCTCAAGAATCAGAG
NM_001146076.1      ------------------------------------------------------------
                                                                                

HsCD00583938        ------------------------------------------------------------
NM_024604.2         TCGGAAGAAGATGGGATTCATGTAGATTCACAAAAGGCTCTTGTTTTAAAAGAAAAGGGC
NM_001146075.1      TCGGAAGAAGATGGGATTCATGTAGATTCACAAAAGGCTCTTGTTTTAAAAGAAAAGGGC
NM_001146076.1      ------------------------------------------------------------
                                                                                

HsCD00583938        ------------------------------------------------CCATCACCATCA
NM_024604.2         AATAAATACTTCAAACAAGGAAAATATGATGAAGCAATTGACTGCTACACAAAAGGCATG
NM_001146075.1      AATAAATACTTCAAACAAGGAAAATATGATGAAGCAATTGACTGCTACACAAAAGGCATG
NM_001146076.1      ---------------------------------------------------------ATG
                                                                             : .

HsCD00583938        CCATCACAGCCATATGACTTCAGCAAATAAAGCAATCGAATTACAACTACAAGTGAAACA
NM_024604.2         GATGCCGATCCATATAATCCCGTGTTGCCAACGAACAGAGCGTCAGCATATTTTAGACTG
NM_001146075.1      GATGCCGATCCATATAATCCCGTGTTGCCAACGAACAGAGCGTCAGCATATTTTAGACTG
NM_001146076.1      GATGCCGATCCATATAATCCCGTGTTGCCAACGAACAGAGCGTCAGCATATTTTAGACTG
                     .: *. * ******.*   *.  ::. .**  ** .**.  :**.*::.:: *..*. .

HsCD00583938        AAATGCAGAAGAATTACAAGACTTTATGCGGGATTTAGAGAACTGGGAAAAAGACATTAA
NM_024604.2         AAAAAATTTGCTGTTGCTGAGTCTGATTGTAATTTAGCAGTTGCCTTGAATAGAAGTTAT
NM_001146075.1      AAAAAATTTGCTGTTGCTGAGTCTGATTGTAATTTAGCAGTTGCCTTGAATAGAAGTTAT
NM_001146076.1      AAAAAATTTGCTGTTGCTGAGTCTGATTGTAATTTAGCAGTTGCCTTGAATAGAAGTTAT
                    ***:..: :. :.**.*:...  * **   ..:**:. **::     .**:***..***:

HsCD00583938        ACAAAAGGATATGGAACTAAGAAGACAGAATGGTGTTCCTGAAGAGAATTTACCTCCTAT
NM_024604.2         ACAAAGGCTTATTCCAGACGAGGTGCTGCTCGATTTGCTTTGCAAAAATTAGAAGAGGCC
NM_001146075.1      ACAAAGGCTTATTCCAGACGAGGTGCTGCTCGATTTGCTTTGCAAAAATTAGAAGAGGCC
NM_001146076.1      ACAAAGGCTTATTCCAGACGAGGTGCTGCTCGATTTGCTTTGCAAAAATTAGAAGAGGCC
                    *****.* :***  .* :..... .*:*.: *.* * * * ...*.****:... .  . 

HsCD00583938        TCGAAATGGGAATTTTAGGAAAAAGAAGAAAGGCAAAGCTAAAGAGTCTTCCAAAAAAAC
NM_024604.2         AAAAAAGATTATGAAAGAGTATTAGAACTAGAACCAAATAACTTTGAAGCAACAAATGAA
NM_001146075.1      AAAAAAGATTATGAAAGAGTATTAGAACTAGAACCAAATAACTTTGAAGCAACAAATGAA
NM_001146076.1      AAAAAAGATTATGAAAGAGTATTAGAACTAGAACCAAATAACTTTGAAGCAACAAATGAA
                    :..*** .  *: :::..*:*::**** :*...*.**. :*.: :*:.  ...***:.*.

HsCD00583938        CAGAGAGGAAAACACAAAAAACAGGATAAAATCTTATGATTATGAGGCATGGGCAAAGCT
NM_024604.2         CTCAGGAAAATCAGTCAGGCTTTAGCATCCAAAGAAAACTCATATCCAAAGG--------
NM_001146075.1      CTCAGGAAAATCAGTCAGGCTTTAGCATCCAAAGAAAACTCATATCCAAAGG--------
NM_001146076.1      CTCAGGAAAATCAGTCAGGCTTTAGCATCCAAAGAAAACTCATATCCAAAGG--------
                    *: **...**:... .*...: :.*.::..*:. :*:..* **.:  .*:**        

HsCD00583938        TGATGTGGACCGTATCCTTGATGAGCTTGACAAAGACGATAGTACCCATGAGTCTCTGTC
NM_024604.2         --------------------------------------AAGCTGACATAGTGATTAAGTC
NM_001146075.1      --------------------------------------AAGCTGACATAGTGATTAAGTC
NM_001146076.1      --------------------------------------AAGCTGACATAGTGATTAAGTC
                                                          *:. *..*.::*:*: *.:***

HsCD00583938        TCAAGAATCAGAGTCGGAGGAAGATGGGATTCATGTAGATTCACAAAAGGCTCTTGTTTT
NM_024604.2         AACAGAAGGAGAGCGAAAGCAAATTGAAGCACAACAGAATAAGCAGCAGGCCATTTCAGA
NM_001146075.1      AACAGAAGGAGAGCGAAAGCAAATTGAAGCACAACAGAATAAGCAGCAGGCCATTTCAGA
NM_001146076.1      AACAGAAGGAGAGCGAAAGCAAATTGAAGCACAACAGAATAAGCAGCAGGCCATTTCAGA
                    :..****  ****  ..** **.:**... :**: :..**:..**..**** .**  : :

HsCD00583938        AAAAGAAAAGGGCAATAAATACTTCAAACAAGGAAAATATGATGAAGCAATTGACTGCTA
NM_024604.2         GAAAGATCGGGGGAATGGATTTTTCAAAGAGGGGAAATATGAAAGAGCAATTGAATGCTA
NM_001146075.1      GAAAGATCGGGGGAATGGATTTTTCAAAGAGGGGAAATATGAAAGAGCAATTGAATGCTA
NM_001146076.1      GAAAGATCGGGGGAATGGATTTTTCAAAGAGGGGAAATATGAAAGAGCAATTGAATGCTA
                    .*****:..*** ***..**: ****** *.**.********:..*********.*****

HsCD00583938        CACAAAAGGCATGGATGCCGATCCATATAATCCCGTGTTGCCAACGAACAGAGCGTCAGC
NM_024604.2         TACTCGAGGGATAGCAGCAGATGGTGCTAATGCCCTTCTTCCAGCTAACAGAGCTATGGC
NM_001146075.1      TACTCGAGGGATAGCAGCAGATGGTGCTAATGCCCTTCTTCCAGCTAACAGAGCTATGGC
NM_001146076.1      TACTCGAGGGATAGCAGCAGATGGTGCTAATGCCCTTCTTCCAGCTAACAGAGCTATGGC
                     **:..*** **.*.:**.***  : .**** ** *  * ***.* ******** : .**

HsCD00583938        ATATTTTAGACTGAAAAAATTTGCTGTTGCTGAGTCTGATTGTAATTTAGCAGTTGCCTT
NM_024604.2         CTATCTGAAGATTCAGAAATATGAAGAAGCTGAAAAAGACTGCACACAAGCCATTTTATT
NM_001146075.1      CTATCTGAAGATTCAGAAATATGAAGAAGCTGAAAAAGACTGCACACAAGCCATTTTATT
NM_001146076.1      CTATCTGAAGATTCAGAAATATGAAGAAGCTGAAAAAGACTGCACACAAGCCATTTTATT
                    .*** * *...* .*.****:**.:*::*****.:.:** ** *.: :***..**  .**

HsCD00583938        GAATAGAAGTTATACAAAGGCTTATTCCAGACGAGGTGCTGCTCGATTTGCTTTGCAAAA
NM_024604.2         AGATGGCTCATATTCTAAAGCTTTTGCCAGAAGAGGAACTGCAAGAACATTTTTGGGAAA
NM_001146075.1      AGATGGCTCATATTCTAAAGCTTTTGCCAGAAGAGGAACTGCAAGAACATTTTTGGGAAA
NM_001146076.1      AGATGGCTCATATTCTAAAGCTTTTGCCAGAAGAGGAACTGCAAGAACATTTTTGGGAAA
                    ..**.*.: :***:*:**.****:* *****.****:.****:.**: :  **** .***

HsCD00583938        ATTAGAAGAGGCCAAAAAAGATTATGAAAGAGTATTAGAACTAGAACCAAATAACTTTGA
NM_024604.2         GCTAAATGAGGCAAAACAAGATTTTGAAACTGTTTTACTTCTGGAACCTGGAAATAAGCA
NM_001146075.1      GCTAAATGAGGCAAAACAAGATTTTGAAACTGTTTTACTTCTGGAACCTGGAAATAAGCA
NM_001146076.1      GCTAAATGAGGCAAAACAAGATTTTGAAACTGTTTTACTTCTGGAACCTGGAAATAAGCA
                    . **.*:*****.***.******:***** :**:*** ::**.*****:..:** ::  *

HsCD00583938        AGCAACAAATGAACTCAGGAAAATCAGTCAGGCTTTAG----------------------
NM_024604.2         AGCAGTAACTGAACTCTCCAAAATTAAAAAGGAATTAATTGAGAAAGGACACTGGGATGA
NM_001146075.1      AGCAGTAACTGAACTCTCCAAAATTAAAAAG-----------------------------
NM_001146076.1      AGCAGTAACTGAACTCTCCAAAATTAAAAAGGAATTAATTGAGAAAGGACACTGGGATGA
                    ****. **.*******:  ***** *.:.**                             

HsCD00583938        ------------------------------------------------------------
NM_024604.2         TGTCTTTCTTGATTCCACACAAAGACAAAATGTGGTAAAACCCATTGATAATCCACCGCA
NM_001146075.1      ------------------------------------------------------------
NM_001146076.1      TGTCTTTCTTGATTCCACACAAAGACAAAATGTGGTAAAACCCATTGATAATCCACCGCA
                                                                                

HsCD00583938        --------------CATCCAAAGAAAACTCATATCCAAAGGAAGCTGACATAGCGATTAA
NM_024604.2         TCCTGGATCAACTAAACCACTCAAGAAGGTTATTATTGAAGAAACTGGTAATTTGATACA
NM_001146075.1      -------------AAACCACTCAAGAAGGTTATTATTGAAGAAACTGGTAATTTGATACA
NM_001146076.1      TCCTGGATCAACTAAACCACTCAAGAAGGTTATTATTGAAGAAACTGGTAATTTGATACA
                                  .* *..:..*.**   :::*. :.*.***.***. *::  ***:.*

HsCD00583938        GTCAACAGAAGGAGAGCGAAAGCAAATTGAAGCACAACAGAATAAGCAGCAGGCCATTTC
NM_024604.2         GACTATTGATGTGCCAGATAGCACTACTGCTGCTGCTCCAGAGAATAATCCTATTAATCT
NM_001146075.1      GACTATTGATGTGCCAGATAGCACTACTGCTGCTGCTCCAGAGAATAATCCTATTAATCT
NM_001146076.1      GACTATTGATGTGCCAGATAGCACTACTGCTGCTGCTCCAGAGAATAATCCTATTAATCT
                    *:*:* :**:* . .. .:*. ..:* **.:**: .:*...* ** .* *. .  *:*  

HsCD00583938        AGAGAAAGATCGGGGGAATGGATTTTTCAAAGAGGGGAAATATGAAAGAGCAATTGAATG
NM_024604.2         AGCAAATGTAATAGCAGCCACAGGCACCACAAGTAAGAAGAATTCAAGCCAAGATGACCT
NM_001146075.1      AGCAAATGTAATAGCAGCCACAGGCACCACAAGTAAGAAGAATTCAAGCCAAGATGACCT
NM_001146076.1      AGCAAATGTAATAGCAGCCACAGGCACCACAAGTAAGAAGAATTCAAGCCAAGATGACCT
                    **..**:*::. .* ... . *   : **.*.. ..***.:** .***. .*.:***.  

HsCD00583938        CTATACTCGAGGGATAGCAGCAGATGGTGCTAATGCCCTTCTTCCAGCTAACAGAGCTAT
NM_024604.2         TTTTCCCACAAGTGATACTCCAAGAGCAAAAGTATTGAAAATAGAAGAAGTCAGTGATAC
NM_001146075.1      TTTTCCCACAAGTGATACTCCAAGAGCAAAAGTATTGAAAATAGAAGAAGTCAGTGATAC
NM_001146076.1      TTTTCCCACAAGTGATACTCCAAGAGCAAAAGTATTGAAAATAGAAGAAGTCAGTGATAC
                     *:*.* . *.* .::.*: **..:* :..:.::   .::.*: .**.:.:***:*.** 

HsCD00583938        GGCCTATCTGAGGATTCAG--------------------------------AAATATGAA
NM_024604.2         TTCATCCCTGCAACCTCAAGCCAGTTTGAAGCAGGATGTATGTCAGTCTTACAGCGAGAA
NM_001146075.1      TTCATCCCTGCAACCTCAAGCCAGTTTGAAGCAGGATGTATGTCAGTCTTACAGCGAGAA
NM_001146076.1      TTCATCCCTGCAACCTCAAGCCAGTTTGAAGCAGGATGTATGTCAGTCTTACAGCGAGAA
                      *.*. ***.... ***.                                .*. .:***

HsCD00583938        GAAGCTGAAAAAGACTGCACACAAGCCATTTTATTAGATGGCTCATATTCTAAAGCTTTT
NM_024604.2         AATGCCCATAGAGATAGAACAAAAACCTGCTCAGTTTGCCACAACTGTTCTTCCTCCAAT
NM_001146075.1      AATGCCCATAGAGATAGAACAAAAACCTGCTCAGTTTGCCACAACTGTTCTTCCTCCAAT
NM_001146076.1      AATGCCCATAGAGATAGAACAAAAACCTGCTCAGTTTGCCACAACTGTTCTTCCTCCAAT
                    .*:**  *:*.*** :*.***.**.**:  * * *: .  .*:..*.****:.. * ::*

HsCD00583938        GCCG-------------------------------------GAAGAGGAACTGCAAGAAC
NM_024604.2         TCCTGCAAACTCGTTCCAGCTCGAATCTGATTTCAGACAATTGAAAAGTTCTCCAGATAT
NM_001146075.1      TCCTGCAAACTCGTTCCAGCTCGAATCTGATTTCAGACAATTGAAAAGTTCTCCAGATAT
NM_001146076.1      TCCTGCAAACTCGTTCCAGCTCGAATCTGATTTCAGACAATTGAAAAGTTCTCCAGATAT
                     **                                       .*.*.*::** **..:* 

HsCD00583938        ATTTTTGGGAAAGCTAAATGAGGCAAAACAAGATTTTGAAACTGTTTTACTTCTGGAACC
NM_024604.2         GTTGTATCAGTATTTAAAGCAAATTGAACCATCTTTGTATCCTAAGTTGTTTCAGAAAAA
NM_001146075.1      GTTGTATCAGTATTTAAAGCAAATTGAACCATCTTTGTATCCTAAGTTGTTTCAGAAAAA
NM_001146076.1      GTTGTATCAGTATTTAAAGCAAATTGAACCATCTTTGTATCCTAAGTTGTTTCAGAAAAA
                    .** *:  ..:*  ****  *.. :.***.* .***  *:.**.: **. ***:*.**..

HsCD00583938        TGGAAATAAGCAAGCAGCAACTGAACTCTCCAAAATTAAAAAGGAATTAATTGAGAAAGG
NM_024604.2         TCTGGATCCAGATGTATTCAACCAGATCGTTAAAATTCTGCATGACTTTTACATTGAGAA
NM_001146075.1      TCTGGATCCAGATGTATTCAACCAGATCGTTAAAATTCTGCATGACTTTTACATTGAGAA
NM_001146076.1      TCTGGATCCAGATGTATTCAACCAGATCGTTAAAATTCTGCATGACTTTTACATTGAGAA
                    *  ..**... *:* *  .*.  *..**   ******.:..* **.**::: .: .*...

HsCD00583938        ACGCTGGGATGATGTCTTTCTTGATTCCACACAAAGACAAAATGTGGTAAAACCCATTGA
NM_024604.2         AGAAAAGCCATTACTCATCTTTGAAATCTTACAAAGACTTTCTGAACTAAAAAGGTTTGA
NM_001146075.1      AGAAAAGCCATTACTCATCTTTGAAATCTTACAAAGACTTTCTGAACTAAAAAGGTTTGA
NM_001146076.1      AGAAAAGCCATTACTCATCTTTGAAATCTTACAAAGACTTTCTGAACTAAAAAGGTTTGA
                    * ..:.* .: :: **:*  ****:: *: ********:::.**:. *****.  :****

HsCD00583938        TAATCCACCGCATCCTGGATCAACTAAACCACTCAAGAAGGTTATTATTGAAGAAACTGG
NM_024604.2         TATGGCAGTGATGTTTATGTCAGAAACAGAGAAAAAGATTGCACGTGCATTATTTAATCA
NM_001146075.1      TATGGCAGTGATGTTTATGTCAGAAACAGAGAAAAAGATTGCACGTGCATTATTTAATCA
NM_001146076.1      TATGGCAGTGATGTTTATGTCAGAAACAGAGAAAAAGATTGCACGTGCATTATTTAATCA
                    **:  **  *.:   *. .***..:*.* ...:.****: * :. *. : :* ::*.* .

HsCD00583938        TAATTTGATGCAGACTATTGATGTGCCAGATAGCACTACTGCTGCTGCTCCAGAGAATTA
NM_024604.2         CATAGACAAGTCAGGATTGAAGGATAGTTCTGTCGAAGAACTCAAGAAAAGATACGGTGG
NM_001146075.1      CATAGACAAGTCAGGATTGAAGGATAGTTCTGTCGAAGAACTCAAGAAAAGATACGGTGG
NM_001146076.1      CATAGACAAGTCAGGATTGAAGGATAGTTCTGTCGAAGAACTCAAGAAAAGATACGGTGG
                     *:: : *:* ... ::* .* *: . : .*. *..:..:   .. ..:. * * ..* .

HsCD00583938        G---
NM_024604.2         TTGA
NM_001146075.1      TTGA
NM_001146076.1      TTGA

Protein

SMART domain :

TPR : Tetratricopeptide repeats

Structural Annotation Wiki :

HR9155

UniProt :

Q9H6T3

HPRD :

07970

Clone

Cloning Information :

629655

Original Clone ID :

HR9155-1-460-15.3

Experimental

TargetTrack - Experimental data :

HR9155

Organism

Species Specific ID:

79657

Genome

Chromosome :

Map Location :

12q13.11

Reagents

Labome :

RPAP3-antibody

RPAP3 (H. sapiens )In pET15_NESG (His-tagged bacterial expression vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Reference Sequence Alignment

Protein

Clone

Experimental

Organism

Genome

Reagents

RPAP3 (H. sapiens )
In pET15_NESG (His-tagged bacterial expression vector)