DNASU Plasmid | NELFCD (H. sapiens )<br>In pET15_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: NELFCD

Gene Name: negative elongation factor complex member C/D

Original Clone ID:

HR9069-15.13

Keyword:

full length protein truncation

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pET15_NESG

Format:

CLOSED

Source:

Northeast Structural Genomics Consortium

Description:

None

Comments:

None

Mutation / Discrepancy:

No / Yes

Publications:

None

Authors:

PSI:Biology
Northeast Structural Genomics Consortium

HsCD00584057

Price:

AVAILABLE

No restriction
Special MTA: PSI/NESG

Insert Sequence
Protein Sequence

Insert sequence: 1773nts

Open reading frame: 12 to 1784

Reference Sequence Alignment

ATCACAGCCATATGGCGGGGGCCGTGCCGGGCGCCATCATGGACGAGGACTACTACAGGA
GCGCGGCCGAGTGGGGCGACGAGGCTGACGGCGGCCAGCAGGAGGATGATTCTGGAGAAG
GAGAGGATGATGCGGAGGTTCAGCAAGAATGCCTGCATAAATTTTCCACCCGGGATTATA
TCATGGAACCCTCCATCTTCAACACTCTGAAGAGGTATTTTCAGGCAGGAGGGTCTCCAG
AGAATGTTATCCAGCTCTTATCTGAAAACAACACCGCTGTGGCCCAGACTGTGAACCTGC
TGGCCGAGTGGCTCATTCAGACAGGTGTTGAGCCAGTGCAGGTTCAGGAAACTGTGGAAA
ATCACTTGAAGAGTTTGCTGATCAAACATTTTGACCCCCGCAAAGCAGATTCTATTTTTA
CTGAAGAAGGAGAGACCCCAGCGTGGCTGGAACAGATGATTGCACATACCACGTGGCGGG
ACCTTTTTTATAAACTGGCTGAAGCCCATCCAGACTGTTTGATGCTGAACTTCACCGTTA
AGCTTATTTCTGACGCAGGGTACCAGGGGGAGATCACCAGTGTGTCCACAGCATGCCAGC
AGCTAGAAGTGTTCTCGAGAGTGCTCCGGACCTCTCTAGCTACAATTTTAGATGGAGGAG
AAGAAAACCTTGAAAAAAATCTCCCTGAGTTTGCTAAGATGGTGTGCCACGGGGAGCACA
CGTATCTGTTTGCCCAGGCCATGATGTCCGTGCTGGCCCAGGAGGAGCAGGGGGGCTCCG
CTGTGCGCAGGATCGCCCAGGAAGTGCAGCGCTTTGCCCAGGAGAAAGGTCATGACGCCA
GTCAGATCACACTAGCCTTGGGCACAGCTGCCTCCTACCCCAGGGCCTGCCAGGCTCTCG
GGGCCATGCTGTCCAAAGGAGCCCTGAACCCTGCTGACATCACCGTCCTGTTCAAGATGT
TCACAAGCATGGACCCTCCTCCGGTTGAACTTATCCGCGTTCCAGCCTTCCTGGACCTGT
TCATGCAGTCACTCTTTAAACCAGGGGCTCGGATCAACCAGGACCACAAGCACAAATACA
TCCACATCTTGGCGTACGCAGCAAGCGTGGTTGAGACCTGGAAGAAGAACAAGCGAGTGA
GCATCAATAAAGATGAGCTGAAGTCAACGTCAAAAGCTGTCGAAACCGTTCACAATTTGT
GTTGCAACGAGAACAAAGGGGCCTCTGAACTAGTGGCAGAATTGAGCACACTTTATCAGT
GTATTAGGTTTCCAGTGGTAGCAATGGGTGTGCTGAAGTGGGTGGATTGGACTGTATCAG
AACCAAGGTACTTTCAGCTGCAGACTGACCATACCCCTGTCCACCTGGCGTTGCTGGATG
AGATCAGCACCTGCCACCAGCTCCTGCACCCCCAGGTCCTGCAGCTGCTTGTTAAGCTTT
TTGAGACTGAGCACTCCCAGCTGGACGTGATGGAGCAGCTTGAGTTGAAGAAGACACTGC
TGGACAGGATGGTTCACCTGCTGAGTCGAGGTTATGTACTTCCTGTTGTCAGTTACATCC
GAAAGTGTCTGGAGAAGCTGGACACTGACATTTCACTCATTCGCTATTTTGTCACTGAGG
TGCTGGACGTCATTGCTCCTCCTTATACCTCTGACTTCGTGCAACTTTTCCTCCCCATCC
TGGAGAATGACAGCATCGCAGGTACCATCAAAACGGAAGGCGAGCATGACCCTGTGACGG
AGTTTATAGCTCACTGCAAATCTAACTTCATCATGGTGAACTAG

Protein sequence predicted based on nucleotide sequence

MAGAVPGAIMDEDYYRSAAEWGDEADGGQQEDDSGEGEDDAEVQQECLHKFSTRDYIMEP
SIFNTLKRYFQAGGSPENVIQLLSENNTAVAQTVNLLAEWLIQTGVEPVQVQETVENHLK
SLLIKHFDPRKADSIFTEEGETPAWLEQMIAHTTWRDLFYKLAEAHPDCLMLNFTVKLIS
DAGYQGEITSVSTACQQLEVFSRVLRTSLATILDGGEENLEKNLPEFAKMVCHGEHTYLF
AQAMMSVLAQEEQGGSAVRRIAQEVQRFAQEKGHDASQITLALGTAASYPRACQALGAML
SKGALNPADITVLFKMFTSMDPPPVELIRVPAFLDLFMQSLFKPGARINQDHKHKYIHIL
AYAASVVETWKKNKRVSINKDELKSTSKAVETVHNLCCNENKGASELVAELSTLYQCIRF
PVVAMGVLKWVDWTVSEPRYFQLQTDHTPVHLALLDEISTCHQLLHPQVLQLLVKLFETE
HSQLDVMEQLELKKTLLDRMVHLLSRGYVLPVVSYIRKCLEKLDTDISLIRYFVTEVLDV
IAPPYTSDFVQLFLPILENDSIAGTIKTEGEHDPVTEFIAHCKSNFIMVN*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

g46a,G16R;t259a,Y87N;

c684t,A228A;c714t,Y238Y

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinPurified: Not_Tested

ProteinExpressed: Protein_Confirmed

SolubleProtein: Tested_Not_Soluble

Find experimental protocols in HR9069

Recommended expression in:

Not Applicable

Vector Name:

pET15_NESG

Synonyms:

pET15, pET15-modified, pET15Cc, pET15_NESG

Sequencing Primer:

Forward: T7

Reverse: T7 terminator

Description:

Bacterial (E. coli) expression vector with T7 lac promoter, adds N-terminal 6x His tag; amp resistance; restriction enzyme cloning.

Comments:

Modified Novagen pET vector-common MCS

Size (bp):

5723

Parent Vector:

None

Properties:

bacterial expression, in vitro transcription, multiple cloning site, with tag/fusion/marker

Author Name:

Gaetano T. Montleione
Jessica Bearden
Northeast Structural Genomics Consortium

Publications:

PMID: 15808222

Title: Robotic cloning and Protein Production Platform of the Northeast Structural Genomics Consortium

Vector Map:

Vector Sequence:

Sequence:

TTCTCATGTTTGACAGCTTATCATCGATAAGCTAGCTTTAATGCGGTAGTTTATCACAGTTAAATTGCTAACGCAGTCAGGCACCGTGTATGAAATCTAACAATGCGCTCATCGTCATCCTCGGCACCGTCACCCTGGATGCTGTAGGCATAGGCTTGGTTATGCCGGTACTGCCGGGCCTCTTGCGGGATATCCGGATATAGTTCCTCCTTTCAGCAAAAAACCCCTCAAGACCCGTTTAGAGGCCCCAAGGGGTTATGCTAGTTATTGCTCAGCGGTGGCAGCAGCCAACTCAGCTTCCTTTCGGGCTTTGTTAGCAGCCGGATCTCGAGAAGCTTTGCGTCGACAGAGCTCGCAGGATCCGCAGAATTCGCCATATGGCTGTGATGGTGATGGTGATGGCCCATGGTATATCTCCTTCTTAAAGTTAAACAAAATTATTTCTAGAGGGGAATTGTTATCCGCTCACAATTCCCCTATAGTGAGTCGTATTAATTTCGCGGGATCGAGATCTCGATCCTCTACGCCGGACGCATCGTGGCCGGCATCACCGGCGCCACAGGTGCGGTTGCTGGCGCCTATATCGCCGACATCACCGATGGGGAAGATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTGTTTCGGCGTGGGTATGGTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCTTGCATGCACCATTCCTTGCGGCGGCGGTGCTCAACGGCCTCAACCTACTACTGGGCTGCTTCCTAATGCAGGAGTCGCATAAGGGAGAGCGTCGAGATCCCGGACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAATGTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAATGCTGAATGAGGGCATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTGCGGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTTATATCCCGCCGTTAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTAAGTTAGCTCACTCATTAGGCACCGGGATCTCGACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACTATCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGCGCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCCTTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTCCCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTGTCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACCAGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGCCTCGGCGAGCACATGGAACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGTCGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACGGATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAACCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCATCTCGGGCAGCGTTGGGTCCTGGCCACGGGTGCGCATGATCGTGCTCCTGTCGTTGAGGACCCGGCTAGGCTGGCGGGGTTGCCTTACTGGTTAGCAGAATGAATCACCGATACGCGAGCGAACGTGAAGCGACTGCTGCTGCAAAACGTCTGCGACCTGAGCAACAACATGAATGGTCTTCGGTTTCCGTGTTTCGTAAAGTCTGGAAACGCGGAAGTCAGCGCCCTGCACCATTATGTTCCGGATCTGCATCGCAGGATGCTGCTGGCTACCCTGTGGAACACCTACATCTGTATTAACGAAGCGCTGGCATTGACCCTGAGTGATTTTTCTCTGGTCCCGCCGCATCCATACCGCCAGTTGTTTACCCTCACAACGTTCCAGTAACCGGGCATGTTCATCATCAGTAACCCGTATCGTGAGCATCCTCTCTCGTTTCATCGGTATCATTACCCCCATGAACAGAAATCCCCCTTACACGGAGGCATCAGTGACCAAACAGGAAAAAACCGCCCTTAACATGGCCCGCTTTATCAGAAGCCAGACATTAACGCTTCTGGAGAAACTCAACGAGCTGGACGCGGATGAACAGGCAGACATCTGTGAATCGCTTCACGACCACGCTGATGAGCTTTACCGCAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTGCAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAACACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTTCAAGAA

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (includes SphI, XbaI, EcoRI, BamHI, XhoI)

327

452

bacterial origin

ori

ColE1 origin of replication

3881

4563

gene fragment

ROP

repressor of primer ROP sequence

3281

3457

primer

T7 forward

T7 forward sequencing primer

481

496

primer

T7 reverse

T7 reverse sequencing primer

257

275

promoter

T7 promoter

478

497

repressor binding site

lacO

lacO regulatory sequence

456

478

selectable marker

AmpR

ampicillin resistance gene

4661

5230

tag

His

N-terminal 6x His tag

387

404

trxn termination sequence

T7 term

T7 termination sequence

257

275

Gene

Gene Symbol :

NELFCD

Symbol Nomenclature :

NELFCD

SYNONYM :

NELF-C; NELF-D; TH1; TH1L

Designation :

Full Nomenclature :

negative elongation factor complex member C/D

GENEID :

51497

Locus Tag :

HSPC130

GenBank Accession :

NM_016397

HGNC :

15934

MIM :

605297

Vega :

OTTHUMG00000032861

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00584057      ----------------ATCACAGCCATATGGCGGGGGCCGTGCCGGGCGCCATCATGGAC
NM_198976.2       ATGCGCCGCGCTCGCTCGCGGGAGGGCATGGCGGGGGCCGTGCCGGGCGCCATCATGGAC
                                  . *. ..  . *********************************

HsCD00584057      GAGGACTACTACAGGAGCGCGGCCGAGTGGGGCGACGAGGCTGACGGCGGCCAGCAGGAG
NM_198976.2       GAGGACTACTACGGGAGCGCGGCCGAGTGGGGCGACGAGGCTGACGGCGGCCAGCAGGAG
                  ************.***********************************************

HsCD00584057      GATGATTCTGGAGAAGGAGAGGATGATGCGGAGGTTCAGCAAGAATGCCTGCATAAATTT
NM_198976.2       GATGATTCTGGAGAAGGAGAGGATGATGCGGAGGTTCAGCAAGAATGCCTGCATAAATTT
                  ************************************************************

HsCD00584057      TCCACCCGGGATTATATCATGGAACCCTCCATCTTCAACACTCTGAAGAGGTATTTTCAG
NM_198976.2       TCCACCCGGGATTATATCATGGAACCCTCCATCTTCAACACTCTGAAGAGGTATTTTCAG
                  ************************************************************

HsCD00584057      GCAGGAGGGTCTCCAGAGAATGTTATCCAGCTCTTATCTGAAAACAACACCGCTGTGGCC
NM_198976.2       GCAGGAGGGTCTCCAGAGAATGTTATCCAGCTCTTATCTGAAAACTACACCGCTGTGGCC
                  *********************************************:**************

HsCD00584057      CAGACTGTGAACCTGCTGGCCGAGTGGCTCATTCAGACAGGTGTTGAGCCAGTGCAGGTT
NM_198976.2       CAGACTGTGAACCTGCTGGCCGAGTGGCTCATTCAGACAGGTGTTGAGCCAGTGCAGGTT
                  ************************************************************

HsCD00584057      CAGGAAACTGTGGAAAATCACTTGAAGAGTTTGCTGATCAAACATTTTGACCCCCGCAAA
NM_198976.2       CAGGAAACTGTGGAAAATCACTTGAAGAGTTTGCTGATCAAACATTTTGACCCCCGCAAA
                  ************************************************************

HsCD00584057      GCAGATTCTATTTTTACTGAAGAAGGAGAGACCCCAGCGTGGCTGGAACAGATGATTGCA
NM_198976.2       GCAGATTCTATTTTTACTGAAGAAGGAGAGACCCCAGCGTGGCTGGAACAGATGATTGCA
                  ************************************************************

HsCD00584057      CATACCACGTGGCGGGACCTTTTTTATAAACTGGCTGAAGCCCATCCAGACTGTTTGATG
NM_198976.2       CATACCACGTGGCGGGACCTTTTTTATAAACTGGCTGAAGCCCATCCAGACTGTTTGATG
                  ************************************************************

HsCD00584057      CTGAACTTCACCGTTAAGCTTATTTCTGACGCAGGGTACCAGGGGGAGATCACCAGTGTG
NM_198976.2       CTGAACTTCACCGTTAAGCTTATTTCTGACGCAGGGTACCAGGGGGAGATCACCAGTGTG
                  ************************************************************

HsCD00584057      TCCACAGCATGCCAGCAGCTAGAAGTGTTCTCGAGAGTGCTCCGGACCTCTCTAGCTACA
NM_198976.2       TCCACAGCATGCCAGCAGCTAGAAGTGTTCTCGAGAGTGCTCCGGACCTCTCTAGCTACA
                  ************************************************************

HsCD00584057      ATTTTAGATGGAGGAGAAGAAAACCTTGAAAAAAATCTCCCTGAGTTTGCTAAGATGGTG
NM_198976.2       ATTTTAGATGGAGGAGAAGAAAACCTTGAAAAAAATCTCCCTGAGTTTGCCAAGATGGTG
                  ************************************************** *********

HsCD00584057      TGCCACGGGGAGCACACGTATCTGTTTGCCCAGGCCATGATGTCCGTGCTGGCCCAGGAG
NM_198976.2       TGCCACGGGGAGCACACGTACCTGTTTGCCCAGGCCATGATGTCCGTGCTGGCCCAGGAG
                  ******************** ***************************************

HsCD00584057      GAGCAGGGGGGCTCCGCTGTGCGCAGGATCGCCCAGGAAGTGCAGCGCTTTGCCCAGGAG
NM_198976.2       GAGCAGGGGGGCTCCGCTGTGCGCAGGATCGCCCAGGAAGTGCAGCGCTTTGCCCAGGAG
                  ************************************************************

HsCD00584057      AAAGGTCATGACGCCAGTCAGATCACACTAGCCTTGGGCACAGCTGCCTCCTACCCCAGG
NM_198976.2       AAAGGTCATGACGCCAGTCAGATCACACTAGCCTTGGGCACAGCTGCCTCCTACCCCAGG
                  ************************************************************

HsCD00584057      GCCTGCCAGGCTCTCGGGGCCATGCTGTCCAAAGGAGCCCTGAACCCTGCTGACATCACC
NM_198976.2       GCCTGCCAGGCTCTCGGGGCCATGCTGTCCAAAGGAGCCCTGAACCCTGCTGACATCACC
                  ************************************************************

HsCD00584057      GTCCTGTTCAAGATGTTCACAAGCATGGACCCTCCTCCGGTTGAACTTATCCGCGTTCCA
NM_198976.2       GTCCTGTTCAAGATGTTCACAAGCATGGACCCTCCTCCGGTTGAACTTATCCGCGTTCCA
                  ************************************************************

HsCD00584057      GCCTTCCTGGACCTGTTCATGCAGTCACTCTTTAAACCAGGGGCTCGGATCAACCAGGAC
NM_198976.2       GCCTTCCTGGACCTGTTCATGCAGTCACTCTTTAAACCAGGGGCTCGGATCAACCAGGAC
                  ************************************************************

HsCD00584057      CACAAGCACAAATACATCCACATCTTGGCGTACGCAGCAAGCGTGGTTGAGACCTGGAAG
NM_198976.2       CACAAGCACAAATACATCCACATCTTGGCGTACGCAGCAAGCGTGGTTGAGACCTGGAAG
                  ************************************************************

HsCD00584057      AAGAACAAGCGAGTGAGCATCAATAAAGATGAGCTGAAGTCAACGTCAAAAGCTGTCGAA
NM_198976.2       AAGAACAAGCGAGTGAGCATCAATAAAGATGAGCTGAAGTCAACGTCAAAAGCTGTCGAA
                  ************************************************************

HsCD00584057      ACCGTTCACAATTTGTGTTGCAACGAGAACAAAGGGGCCTCTGAACTAGTGGCAGAATTG
NM_198976.2       ACCGTTCACAATTTGTGTTGCAACGAGAACAAAGGGGCCTCTGAACTAGTGGCAGAATTG
                  ************************************************************

HsCD00584057      AGCACACTTTATCAGTGTATTAGGTTTCCAGTGGTAGCAATGGGTGTGCTGAAGTGGGTG
NM_198976.2       AGCACACTTTATCAGTGTATTAGGTTTCCAGTGGTAGCAATGGGTGTGCTGAAGTGGGTG
                  ************************************************************

HsCD00584057      GATTGGACTGTATCAGAACCAAGGTACTTTCAGCTGCAGACTGACCATACCCCTGTCCAC
NM_198976.2       GATTGGACTGTATCAGAACCAAGGTACTTTCAGCTGCAGACTGACCATACCCCTGTCCAC
                  ************************************************************

HsCD00584057      CTGGCGTTGCTGGATGAGATCAGCACCTGCCACCAGCTCCTGCACCCCCAGGTCCTGCAG
NM_198976.2       CTGGCGTTGCTGGATGAGATCAGCACCTGCCACCAGCTCCTGCACCCCCAGGTCCTGCAG
                  ************************************************************

HsCD00584057      CTGCTTGTTAAGCTTTTTGAGACTGAGCACTCCCAGCTGGACGTGATGGAGCAGCTTGAG
NM_198976.2       CTGCTTGTTAAGCTTTTTGAGACTGAGCACTCCCAGCTGGACGTGATGGAGCAGCTTGAG
                  ************************************************************

HsCD00584057      TTGAAGAAGACACTGCTGGACAGGATGGTTCACCTGCTGAGTCGAGGTTATGTACTTCCT
NM_198976.2       TTGAAGAAGACACTGCTGGACAGGATGGTTCACCTGCTGAGTCGAGGTTATGTACTTCCT
                  ************************************************************

HsCD00584057      GTTGTCAGTTACATCCGAAAGTGTCTGGAGAAGCTGGACACTGACATTTCACTCATTCGC
NM_198976.2       GTTGTCAGTTACATCCGAAAGTGTCTGGAGAAGCTGGACACTGACATTTCACTCATTCGC
                  ************************************************************

HsCD00584057      TATTTTGTCACTGAGGTGCTGGACGTCATTGCTCCTCCTTATACCTCTGACTTCGTGCAA
NM_198976.2       TATTTTGTCACTGAGGTGCTGGACGTCATTGCTCCTCCTTATACCTCTGACTTCGTGCAA
                  ************************************************************

HsCD00584057      CTTTTCCTCCCCATCCTGGAGAATGACAGCATCGCAGGTACCATCAAAACGGAAGGCGAG
NM_198976.2       CTTTTCCTCCCCATCCTGGAGAATGACAGCATCGCAGGTACCATCAAAACGGAAGGCGAG
                  ************************************************************

HsCD00584057      CATGACCCTGTGACGGAGTTTATAGCTCACTGCAAATCTAACTTCATCATGGTGAACTAG
NM_198976.2       CATGACCCTGTGACGGAGTTTATAGCTCACTGCAAATCTAACTTCATCATGGTGAACTAA
                  ***********************************************************.

Pathways

Reactome :

Abortive elongation of HIV-1 transcript in the absence of Tat

Reactome :

Disease

Reactome :

Elongation arrest and recovery

Reactome :

Formation of HIV elongation complex in the absence of HIV Tat

Reactome :

Formation of HIV-1 elongation complex containing HIV-1 Tat

Reactome :

Formation of RNA Pol II elongation complex

Reactome :

Formation of the Early Elongation Complex

Reactome :

Formation of the HIV-1 Early Elongation Complex

Reactome :

Gene Expression

Reactome :

HIV elongation arrest and recovery

Reactome :

HIV Infection

Reactome :

HIV Life Cycle

Reactome :

HIV Transcription Elongation

Reactome :

Late Phase of HIV Life Cycle

Reactome :

Pausing and recovery of HIV elongation

Reactome :

Pausing and recovery of Tat-mediated HIV elongation

Reactome :

RNA Polymerase II Pre-transcription Events

Reactome :

RNA Polymerase II Transcription

Reactome :

RNA Polymerase II Transcription Elongation

Reactome :

Tat-mediated elongation of the HIV-1 transcript

Reactome :

Tat-mediated HIV elongation arrest and recovery

Reactome :

Transcription

Reactome :

Transcription of the HIV genome

WikiPathway :

Epithelium TarBase

WikiPathway :

Leukocyte TarBase

WikiPathway :

Lymphocyte TarBase

WikiPathway :

Muscle cell TarBase

Protein

Structural Annotation Wiki :

HR9069

UniProt :

Q8IXH7

HPRD :

16096

Clone

Cloning Information :

629612

Original Clone ID :

HR9069-15.13

Experimental

TargetTrack - Experimental data :

HR9069

Organism

Species Specific ID:

51497

Genome

Chromosome :

Map Location :

20q13

Ensembl :

ENSG00000101158

Reagents

Labome :

NELFCD-antibody

NELFCD (H. sapiens )In pET15_NESG (His-tagged bacterial expression vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Reference Sequence Alignment

Pathways

Protein

Clone

Experimental

Organism

Genome

Reagents

NELFCD (H. sapiens )
In pET15_NESG (His-tagged bacterial expression vector)