DNASU Plasmid | ZBTB12 (H. sapiens )<br>In pET15_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: ZBTB12

Gene Name: zinc finger and BTB domain containing 12

Original Clone ID:

HR4454E-366-418-14.3

Keyword:

targeted domain

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pET15_NESG

Format:

CLOSED

Source:

Northeast Structural Genomics Consortium

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

PSI:Biology
Northeast Structural Genomics Consortium

HsCD00585229

Price:

AVAILABLE

No restriction
Special MTA: PSI/NESG

Insert Sequence
Protein Sequence

Insert sequence: 165nts

Open reading frame: 31 to 195

Reference Sequence Alignment

ATGGGCCATCACCATCACCATCACAGCCATATGAAGCAGTTCAACCACAGCAGCAACCTC
AACCGCCACATGAACGTGCATCGTGGTGTCAAGTCACACTCGTGCGGCATCTGCGGCAAG
TGCTTCACACAGAAGTCCACCCTTCACGACCACCTCAACCTGCACTCGGGAGCGCGGCCC
TACCGCTGCTCCTAG

Protein sequence predicted based on nucleotide sequence

MKQFNHSSNLNRHMNVHRGVKSHSCGICGKCFTQKSTLHDHLNLHSGARPYRCS*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinPurified: Not_Tested

ProteinExpressed: Protein_Confirmed

SolubleProtein: Protein_Soluble

Find experimental protocols in HR4454E

Recommended expression in:

Not Applicable

Vector Name:

pET15_NESG

Synonyms:

pET15, pET15-modified, pET15Cc, pET15_NESG

Sequencing Primer:

Forward: T7

Reverse: T7 terminator

Description:

Bacterial (E. coli) expression vector with T7 lac promoter, adds N-terminal 6x His tag; amp resistance; restriction enzyme cloning.

Comments:

Modified Novagen pET vector-common MCS

Size (bp):

5723

Parent Vector:

None

Properties:

bacterial expression, in vitro transcription, multiple cloning site, with tag/fusion/marker

Author Name:

Gaetano T. Montleione
Jessica Bearden
Northeast Structural Genomics Consortium

Publications:

PMID: 15808222

Title: Robotic cloning and Protein Production Platform of the Northeast Structural Genomics Consortium

Vector Map:

Vector Sequence:

Sequence:

TTCTCATGTTTGACAGCTTATCATCGATAAGCTAGCTTTAATGCGGTAGTTTATCACAGTTAAATTGCTAACGCAGTCAGGCACCGTGTATGAAATCTAACAATGCGCTCATCGTCATCCTCGGCACCGTCACCCTGGATGCTGTAGGCATAGGCTTGGTTATGCCGGTACTGCCGGGCCTCTTGCGGGATATCCGGATATAGTTCCTCCTTTCAGCAAAAAACCCCTCAAGACCCGTTTAGAGGCCCCAAGGGGTTATGCTAGTTATTGCTCAGCGGTGGCAGCAGCCAACTCAGCTTCCTTTCGGGCTTTGTTAGCAGCCGGATCTCGAGAAGCTTTGCGTCGACAGAGCTCGCAGGATCCGCAGAATTCGCCATATGGCTGTGATGGTGATGGTGATGGCCCATGGTATATCTCCTTCTTAAAGTTAAACAAAATTATTTCTAGAGGGGAATTGTTATCCGCTCACAATTCCCCTATAGTGAGTCGTATTAATTTCGCGGGATCGAGATCTCGATCCTCTACGCCGGACGCATCGTGGCCGGCATCACCGGCGCCACAGGTGCGGTTGCTGGCGCCTATATCGCCGACATCACCGATGGGGAAGATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTGTTTCGGCGTGGGTATGGTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCTTGCATGCACCATTCCTTGCGGCGGCGGTGCTCAACGGCCTCAACCTACTACTGGGCTGCTTCCTAATGCAGGAGTCGCATAAGGGAGAGCGTCGAGATCCCGGACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAATGTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAATGCTGAATGAGGGCATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTGCGGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTTATATCCCGCCGTTAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTAAGTTAGCTCACTCATTAGGCACCGGGATCTCGACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACTATCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGCGCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCCTTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTCCCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTGTCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACCAGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGCCTCGGCGAGCACATGGAACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGTCGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACGGATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAACCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCATCTCGGGCAGCGTTGGGTCCTGGCCACGGGTGCGCATGATCGTGCTCCTGTCGTTGAGGACCCGGCTAGGCTGGCGGGGTTGCCTTACTGGTTAGCAGAATGAATCACCGATACGCGAGCGAACGTGAAGCGACTGCTGCTGCAAAACGTCTGCGACCTGAGCAACAACATGAATGGTCTTCGGTTTCCGTGTTTCGTAAAGTCTGGAAACGCGGAAGTCAGCGCCCTGCACCATTATGTTCCGGATCTGCATCGCAGGATGCTGCTGGCTACCCTGTGGAACACCTACATCTGTATTAACGAAGCGCTGGCATTGACCCTGAGTGATTTTTCTCTGGTCCCGCCGCATCCATACCGCCAGTTGTTTACCCTCACAACGTTCCAGTAACCGGGCATGTTCATCATCAGTAACCCGTATCGTGAGCATCCTCTCTCGTTTCATCGGTATCATTACCCCCATGAACAGAAATCCCCCTTACACGGAGGCATCAGTGACCAAACAGGAAAAAACCGCCCTTAACATGGCCCGCTTTATCAGAAGCCAGACATTAACGCTTCTGGAGAAACTCAACGAGCTGGACGCGGATGAACAGGCAGACATCTGTGAATCGCTTCACGACCACGCTGATGAGCTTTACCGCAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTGCAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAACACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTTCAAGAA

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (includes SphI, XbaI, EcoRI, BamHI, XhoI)

327

452

bacterial origin

ori

ColE1 origin of replication

3881

4563

gene fragment

ROP

repressor of primer ROP sequence

3281

3457

primer

T7 forward

T7 forward sequencing primer

481

496

primer

T7 reverse

T7 reverse sequencing primer

257

275

promoter

T7 promoter

478

497

repressor binding site

lacO

lacO regulatory sequence

456

478

selectable marker

AmpR

ampicillin resistance gene

4661

5230

tag

His

N-terminal 6x His tag

387

404

trxn termination sequence

T7 term

T7 termination sequence

257

275

Gene

Protein

Clone

Experimental

Organism

Genome
Reagents

Gene

Gene Symbol :

ZBTB12

Symbol Nomenclature :

ZBTB12

SYNONYM :

Bat9; C6orf46; D6S59E; G10; NG35

Designation :

HLA-B-associated transcript 9|zinc finger and BTB domain-containing protein 12

Full Nomenclature :

zinc finger and BTB domain containing 12

GENEID :

221527

Locus Tag :

DADB-122G4.2

GI :

32469509

GenBank Accession :

NM_181842

HGNC :

19066

Vega :

OTTHUMG00000031169

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00585229      ------------------------------------------------------------
NM_181842.2       ATGGCCTCTGGGGTGGAAGTCCTGCGCTTCCAGCTGCCCGGCCACGAGGCCGCAACGCTA
                                                                              

HsCD00585229      ------------------------------------------------------------
NM_181842.2       CGGAACATGAACCAGCTCCGGGCAGAGGAGCGGTTCTGCGACGTGACCATTGTGGCCGAC
                                                                              

HsCD00585229      ------------------------------------------------------------
NM_181842.2       AGCCTCAAGTTTCGAGGCCACAAGGTCATCTTGGCCGCCTGCTCACCCTTCCTGCGGGAC
                                                                              

HsCD00585229      ------------------------------------------------------------
NM_181842.2       CAGTTCCTGCTGAACCCCAGCTCGGAGCTGCAGGTCTCCCTGATGCACAGTGCACGCATC
                                                                              

HsCD00585229      ------------------------------------------------------------
NM_181842.2       GTGGCCGACTTGCTCCTCTCCTGCTACACGGGCGCCTTGGAATTCGCTGTTAGGGACATC
                                                                              

HsCD00585229      ------------------------------------------------------------
NM_181842.2       GTCAACTACCTTACAGCCGCCTCCTACCTGCAGATGGAGCACGTGGTGGAGAAATGCCGG
                                                                              

HsCD00585229      ------------------------------------------------------------
NM_181842.2       AATGCCCTCAGCCAGTTCATTGAGCCCAAAATAGGCCTCAAAGAGGATGGGGTCAGTGAG
                                                                              

HsCD00585229      ------------------------------------------------------------
NM_181842.2       GCTAGCCTTGTGAGCAGCATCAGCGCCACCAAGTCCCTCCTCCCTCCAGCCAGGACCCCA
                                                                              

HsCD00585229      ------------------------------------------------------------
NM_181842.2       AAGCCAGCCCCGAAGCCCCCACCCCCACCTCCTCTACCCCCTCCACTCCTGCGGCCAGTG
                                                                              

HsCD00585229      ------------------------------------------------------------
NM_181842.2       AAGCTGGAGTTCCCACTGGATGAAGACTTGGAGCTGAAAGCCGAGGAAGAGGATGAGGAT
                                                                              

HsCD00585229      ------------------------------------------------------------
NM_181842.2       GAGGATGAGGACGTGTCTGACATCTGCATCGTCAAGGTGGAGTCGGCCCTGGAGGTGGCA
                                                                              

HsCD00585229      ------------------------------------------------------------
NM_181842.2       CACCGGCTCAAACCCCCTGGAGGCCTGGGAGGGGGTCTGGGCATTGGAGGCTCCGTGGGT
                                                                              

HsCD00585229      ------------------------------------------------------------
NM_181842.2       GGCCACCTTGGGGAGCTGGCCCAGAGCAGCGTTCCCCCCAGCACTGTAGCCCCACCGCAG
                                                                              

HsCD00585229      ------------------------------------------------------------
NM_181842.2       GGTGTGGTGAAGGCCTGCTATAGCCTGTCGGAAGATGCAGAAGGGGAGGGCCTGCTGTTG
                                                                              

HsCD00585229      ------------------------------------------------------------
NM_181842.2       ATTCCCGGAGGCCGGGCCAGCGTGGGGGCCACCTCGGGCCTGGTGGAAGCAGCAGCGGTG
                                                                              

HsCD00585229      ------------------------------------------------------------
NM_181842.2       GCCATGGCTGCCCGGGGGGCGGGGGGCAGCCTGGGGGCGGGGGGCAGCCGGGGACCCCTG
                                                                              

HsCD00585229      ------------------------------------------------------------
NM_181842.2       CCTGGGGGCTTCTCAGGTGGAAACCCCTTAAAGAACATCAAGTGCACCAAGTGCCCGGAA
                                                                              

HsCD00585229      ------------------------------------------ATGGGCCATCACCATCAC
NM_181842.2       GTGTTCCAGGGCGTGGAGAAGCTGGTCTTCCACATGCGGGCGCAGCACTTCATCTTCATG
                                                            .:* .* : .:* : .: 

HsCD00585229      CATCACAGCCATATGAAGCAGTTCAACCACAGCAGCAACCTCAACCGCCACATGAACGTG
NM_181842.2       TGCCCTCGCTGTGGCAAGCAGTTCAACCACAGCAGCAACCTCAACCGCCACATGAACGTG
                   . *. .** .*.  *********************************************

HsCD00585229      CATCGTGGTGTCAAGTCACACTCGTGCGGCATCTGCGGCAAGTGCTTCACACAGAAGTCC
NM_181842.2       CATCGTGGTGTCAAGTCACACTCGTGCGGCATCTGCGGCAAGTGCTTCACACAGAAGTCC
                  ************************************************************

HsCD00585229      ACCCTTCACGACCACCTCAACCTGCACTCGGGAGCGCGGCCCTACCGCTGCTCCTAG---
NM_181842.2       ACCCTTCACGACCACCTCAACCTGCACTCGGGAGCGCGGCCCTACCGCTGCTCCTACTGC
                  ********************************************************    

HsCD00585229      ------------------------------------------------------------
NM_181842.2       GACGTGCGCTTCGCCCACAAGCCTGCCATTAGGCGGCACCTCAAGGAGCAACACGGCAAG
                                                                              

HsCD00585229      ------------------------------------------------------------
NM_181842.2       ACCACGGCCGAGAACGTGCTGGAGGCCAGTGTGGCCGAGATTAACGTCCTCATCCGCTAG

Protein

Structural Annotation Wiki :

HR4454E

UniProt :

Q9Y330

HPRD :

15691

PDBe Mol* Structure Viewer

Clone

Cloning Information :

630118

Original Clone ID :

HR4454E-366-418-14.3

Experimental

TargetTrack - Experimental data :

HR4454E

Organism

Species Specific ID:

221527

Genome

Chromosome :

Map Location :

6p21.33

Ensembl :

ENSG00000204366

Reagents

Labome :

ZBTB12-antibody

ZBTB12 (H. sapiens )In pET15_NESG (His-tagged bacterial expression vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Reference Sequence Alignment

Protein

PDBe Mol* Structure Viewer

Clone

Experimental

Organism

Genome

Reagents

ZBTB12 (H. sapiens )
In pET15_NESG (His-tagged bacterial expression vector)