DNASU Plasmid | PPP2R5A (H. sapiens )<br>In pET15_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: PPP2R5A

Gene Name: protein phosphatase 2, regulatory subunit B', alpha

Original Clone ID:

HR3092-19-397-14.9

Keyword:

targeted domain

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pET15_NESG

Format:

FUSION

Source:

Northeast Structural Genomics Consortium

Description:

None

Comments:

None

Mutation / Discrepancy:

No / Yes

Publications:

None

Authors:

PSI:Biology
Northeast Structural Genomics Consortium

HsCD00585861

Price:

AVAILABLE

No restriction
Special MTA: PSI/NESG

Insert Sequence
Protein Sequence

Insert sequence: 1137nts

Open reading frame: 15 to 1151

Reference Sequence Alignment

ACCATCACAGCCATATGGAGAAAGTGGACGGCTTCACCCGGAAATCGGTCCGCAAGGCGC
AGAGGCAGAAGCGCTCCCAGGGCTCGTCGCAGTTTCGCAGCCAGGGCAGCCAGGCAGAGC
TGCACTCGCTGCCCCAGCTCAAAGATGCCACTTCAAATGAACAACAAGAGCTTTTCTGTC
AGAAGTTGCAGCAGTGTTGTATACTGTTTGATTTCATGGACTCTGTTTCAGACTTGAAGA
GCAAAGAAATTAAAAGAGCAACACTGAATGAACTGGTTGAGTATGTTTCAACTAATCGTG
GTGTAATTGTTGAATCAGCGTATTCTGATATAGTAAAAATGATCAGTGCTAACATCTTCC
GTACACTTCCTCCAAGTGATAATCCAGATTCTGATCCAGAAGAGGATGAACCCACGCTTG
GGGCCTCTTGGCCTCACATACAGTTGGTATATGGATTCTTCTTGAGATTTTTGGAGAGCC
CTGATTTCCAGCCCAGCATTGCAAAACGATACATTGATCAGAAATTCGTACAACAGCTCC
TGGAGCTTTTTGATAGTGAAGATCCCAGAGAACGTGACTTCCTGAAGACTGTTCTGCACC
GAATTTATGGGAAATTTCTTGGATTAAGAGCATTCATCAGAAAACAAATTAACAACATTT
TCCTCAGGTTTATATATGAAACAGAACATTTCAATGGTGTTGCTGAACTTCTTGAAATAT
TAGGAAGTATTACCAATGGCTTTGCATTGCCACTGAAAGCAGAACATAAACAATTTCTAA
TGAAGGTTCTTATTCCTATGCATACTGCAAAAGGATTAGCTTTGTTTCATGCTCAGCTAG
CATATTGTGTTGTACAGTTCCTGGAGAAAGATACAACACTAACAGAGCCAGTGATCAGAG
GACTGCTGAAATTTTGGTCAAAAACCTGCAGTCAGAAAGAGGTGATGTTTTTAGGAGAAA
TTGAAGAAATCTTAGATGTCATTGAACCAACACAGTTCAAAAAAATTGAAGAGCCACTTT
TCAAGCAGATATCCAAGTGTGTATCCAGTTCTCGTTTTCAGGTTGCAGAAAGGGCATTGT
ACTTCTGGAATAACGAATATATTCTTAGTTTGATTGAGGAGAACATTGATAAAATTCTGC
CAATTATGTTTTGCCTAG

Protein sequence predicted based on nucleotide sequence

MEKVDGFTRKSVRKAQRQKRSQGSSQFRSQGSQAELHSLPQLKDATSNEQQELFCQKLQQ
CCILFDFMDSVSDLKSKEIKRATLNELVEYVSTNRGVIVESAYSDIVKMISANIFRTLPP
SDNPDSDPEEDEPTLGASWPHIQLVYGFFLRFLESPDFQPSIAKRYIDQKFVQQLLELFD
SEDPRERDFLKTVLHRIYGKFLGLRAFIRKQINNIFLRFIYETEHFNGVAELLEILGSIT
NGFALPLKAEHKQFLMKVLIPMHTAKGLALFHAQLAYCVVQFLEKDTTLTEPVIRGLLKF
WSKTCSQKEVMFLGEIEEILDVIEPTQFKKIEEPLFKQISKCVSSSRFQVAERALYFWNN
EYILSLIEENIDKILPIMF

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

c112t,P38S;t377c,F126S;

a407g,E136G;a440g,E147G;

t480c,P160P;t719c,I240T;

c904t,P302S;a1040g,H347R

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinPurified: Not_Tested

ProteinExpressed: Protein_Confirmed

SolubleProtein: Tested_Not_Soluble

Find experimental protocols in HR3092

Recommended expression in:

Not Applicable

Vector Name:

pET15_NESG

Synonyms:

pET15, pET15-modified, pET15Cc, pET15_NESG

Sequencing Primer:

Forward: T7

Reverse: T7 terminator

Description:

Bacterial (E. coli) expression vector with T7 lac promoter, adds N-terminal 6x His tag; amp resistance; restriction enzyme cloning.

Comments:

Modified Novagen pET vector-common MCS

Size (bp):

5723

Parent Vector:

None

Properties:

bacterial expression, in vitro transcription, multiple cloning site, with tag/fusion/marker

Author Name:

Gaetano T. Montleione
Jessica Bearden
Northeast Structural Genomics Consortium

Publications:

PMID: 15808222

Title: Robotic cloning and Protein Production Platform of the Northeast Structural Genomics Consortium

Vector Map:

Vector Sequence:

Sequence:

TTCTCATGTTTGACAGCTTATCATCGATAAGCTAGCTTTAATGCGGTAGTTTATCACAGTTAAATTGCTAACGCAGTCAGGCACCGTGTATGAAATCTAACAATGCGCTCATCGTCATCCTCGGCACCGTCACCCTGGATGCTGTAGGCATAGGCTTGGTTATGCCGGTACTGCCGGGCCTCTTGCGGGATATCCGGATATAGTTCCTCCTTTCAGCAAAAAACCCCTCAAGACCCGTTTAGAGGCCCCAAGGGGTTATGCTAGTTATTGCTCAGCGGTGGCAGCAGCCAACTCAGCTTCCTTTCGGGCTTTGTTAGCAGCCGGATCTCGAGAAGCTTTGCGTCGACAGAGCTCGCAGGATCCGCAGAATTCGCCATATGGCTGTGATGGTGATGGTGATGGCCCATGGTATATCTCCTTCTTAAAGTTAAACAAAATTATTTCTAGAGGGGAATTGTTATCCGCTCACAATTCCCCTATAGTGAGTCGTATTAATTTCGCGGGATCGAGATCTCGATCCTCTACGCCGGACGCATCGTGGCCGGCATCACCGGCGCCACAGGTGCGGTTGCTGGCGCCTATATCGCCGACATCACCGATGGGGAAGATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTGTTTCGGCGTGGGTATGGTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCTTGCATGCACCATTCCTTGCGGCGGCGGTGCTCAACGGCCTCAACCTACTACTGGGCTGCTTCCTAATGCAGGAGTCGCATAAGGGAGAGCGTCGAGATCCCGGACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAATGTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAATGCTGAATGAGGGCATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTGCGGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTTATATCCCGCCGTTAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTAAGTTAGCTCACTCATTAGGCACCGGGATCTCGACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACTATCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGCGCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCCTTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTCCCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTGTCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACCAGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGCCTCGGCGAGCACATGGAACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGTCGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACGGATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAACCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCATCTCGGGCAGCGTTGGGTCCTGGCCACGGGTGCGCATGATCGTGCTCCTGTCGTTGAGGACCCGGCTAGGCTGGCGGGGTTGCCTTACTGGTTAGCAGAATGAATCACCGATACGCGAGCGAACGTGAAGCGACTGCTGCTGCAAAACGTCTGCGACCTGAGCAACAACATGAATGGTCTTCGGTTTCCGTGTTTCGTAAAGTCTGGAAACGCGGAAGTCAGCGCCCTGCACCATTATGTTCCGGATCTGCATCGCAGGATGCTGCTGGCTACCCTGTGGAACACCTACATCTGTATTAACGAAGCGCTGGCATTGACCCTGAGTGATTTTTCTCTGGTCCCGCCGCATCCATACCGCCAGTTGTTTACCCTCACAACGTTCCAGTAACCGGGCATGTTCATCATCAGTAACCCGTATCGTGAGCATCCTCTCTCGTTTCATCGGTATCATTACCCCCATGAACAGAAATCCCCCTTACACGGAGGCATCAGTGACCAAACAGGAAAAAACCGCCCTTAACATGGCCCGCTTTATCAGAAGCCAGACATTAACGCTTCTGGAGAAACTCAACGAGCTGGACGCGGATGAACAGGCAGACATCTGTGAATCGCTTCACGACCACGCTGATGAGCTTTACCGCAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTGCAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAACACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTTCAAGAA

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (includes SphI, XbaI, EcoRI, BamHI, XhoI)

327

452

bacterial origin

ori

ColE1 origin of replication

3881

4563

gene fragment

ROP

repressor of primer ROP sequence

3281

3457

primer

T7 forward

T7 forward sequencing primer

481

496

primer

T7 reverse

T7 reverse sequencing primer

257

275

promoter

T7 promoter

478

497

repressor binding site

lacO

lacO regulatory sequence

456

478

selectable marker

AmpR

ampicillin resistance gene

4661

5230

tag

His

N-terminal 6x His tag

387

404

trxn termination sequence

T7 term

T7 termination sequence

257

275

Gene

Gene Symbol :

PPP2R5A

Symbol Nomenclature :

PPP2R5A

SYNONYM :

B56A; PR61A

Designation :

PP2A B subunit isoform B'-alpha|PP2A B subunit isoform B56-alpha|PP2A B subunit isoform PR61-alpha|PP2A B subunit isoform R5-alpha|PP2A, B subunit, B' alpha isoform|PP2A, B subunit, B56 alpha isoform|PP2A, B subunit, PR61 alpha isoform|PP2A, B subunit, R5 alpha isoform|PR61alpha|protein phosphatase 2, regulatory subunit B (B56), alpha isoform|protein phosphatase 2, regulatory subunit B', alpha isoform|serine/threonine protein phosphatase 2A, 56 kDa regulatory subunit, alpha isoform|serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit alpha isoform

Full Nomenclature :

protein phosphatase 2, regulatory subunit B', alpha

GENEID :

5525

GI :

5453950

GenBank Accession :

NM_006243

HGNC :

9309

MIM :

601643

Vega :

OTTHUMG00000036750

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00585861        -------------------------------------ACCATCACAGCCATATGGAGAAA
NM_001199756.1      ------------------------------------------------------------
NM_006243.3         ATGTCGTCGTCGTCGCCGCCGGCGGGGGCTGCCAGCGCCGCCATCTCGGCCTCGGAGAAA
                                                                                

HsCD00585861        GTGGACGGCTTCACCCGGAAATCGGTCCGCAAGGCGCAGAGGCAGAAGCGCTCCCAGGGC
NM_001199756.1      ------------------------------------------------------------
NM_006243.3         GTGGACGGCTTCACCCGGAAATCGGTCCGCAAGGCGCAGAGGCAGAAGCGCTCCCAGGGC
                                                                                

HsCD00585861        TCGTCGCAGTTTCGCAGCCAGGGCAGCCAGGCAGAGCTGCACTCGCTGCCCCAGCTCAAA
NM_001199756.1      ---------------------------------------------------ATGATAATG
NM_006243.3         TCGTCGCAGTTTCGCAGCCAGGGCAGCCAGGCAGAGCTGCACCCGCTGCCCCAGCTCAAA
                                                                       .:*.*.*:.

HsCD00585861        GATGCCACTTCAAATGAACAACAAGAGCTTTTCTGTCAGAAGTTGCAGCAGTGTTGTATA
NM_001199756.1      AATGCCACTTCAAATGAACAACAAGAGCTTTTCTGTCAGAAGTTGCAGCAGTGTTGTATA
NM_006243.3         GATGCCACTTCAAATGAACAACAAGAGCTTTTCTGTCAGAAGTTGCAGCAGTGTTGTATA
                    .***********************************************************

HsCD00585861        CTGTTTGATTTCATGGACTCTGTTTCAGACTTGAAGAGCAAAGAAATTAAAAGAGCAACA
NM_001199756.1      CTGTTTGATTTCATGGACTCTGTTTCAGACTTGAAGAGCAAAGAAATTAAAAGAGCAACA
NM_006243.3         CTGTTTGATTTCATGGACTCTGTTTCAGACTTGAAGAGCAAAGAAATTAAAAGAGCAACA
                    ************************************************************

HsCD00585861        CTGAATGAACTGGTTGAGTATGTTTCAACTAATCGTGGTGTAATTGTTGAATCAGCGTAT
NM_001199756.1      CTGAATGAACTGGTTGAGTATGTTTCAACTAATCGTGGTGTAATTGTTGAATCAGCGTAT
NM_006243.3         CTGAATGAACTGGTTGAGTATGTTTCAACTAATCGTGGTGTAATTGTTGAATCAGCGTAT
                    ************************************************************

HsCD00585861        TCTGATATAGTAAAAATGATCAGTGCTAACATCTTCCGTACACTTCCTCCAAGTGATAAT
NM_001199756.1      TCTGATATAGTAAAAATGATCAGTGCTAACATCTTCCGTACACTTCCTCCAAGTGATAAT
NM_006243.3         TCTGATATAGTAAAAATGATCAGTGCTAACATCTTCCGTACACTTCCTCCAAGTGATAAT
                    ************************************************************

HsCD00585861        CCAGATTCTGATCCAGAAGAGGATGAACCCACGCTTGGGGCCTCTTGGCCTCACATACAG
NM_001199756.1      CCAGATTTTGATCCAGAAGAGGATGAACCCACGCTTGAGGCCTCTTGGCCTCACATACAG
NM_006243.3         CCAGATTTTGATCCAGAAGAGGATGAACCCACGCTTGAGGCCTCTTGGCCTCACATACAG
                    ******* *****************************.**********************

HsCD00585861        TTGGTATATGGATTCTTCTTGAGATTTTTGGAGAGCCCTGATTTCCAGCCCAGCATTGCA
NM_001199756.1      TTGGTATATGAATTCTTCTTGAGATTTTTGGAGAGCCCTGATTTCCAGCCTAGCATTGCA
NM_006243.3         TTGGTATATGAATTCTTCTTGAGATTTTTGGAGAGCCCTGATTTCCAGCCTAGCATTGCA
                    **********.*************************************** *********

HsCD00585861        AAACGATACATTGATCAGAAATTCGTACAACAGCTCCTGGAGCTTTTTGATAGTGAAGAT
NM_001199756.1      AAACGATACATTGATCAGAAATTCGTACAACAGCTCCTGGAGCTTTTTGATAGTGAAGAT
NM_006243.3         AAACGATACATTGATCAGAAATTCGTACAACAGCTCCTGGAGCTTTTTGATAGTGAAGAT
                    ************************************************************

HsCD00585861        CCCAGAGAACGTGACTTCCTGAAGACTGTTCTGCACCGAATTTATGGGAAATTTCTTGGA
NM_001199756.1      CCCAGAGAACGTGACTTCCTGAAGACTGTTCTGCACCGAATTTATGGGAAATTTCTTGGA
NM_006243.3         CCCAGAGAACGTGACTTCCTGAAGACTGTTCTGCACCGAATTTATGGGAAATTTCTTGGA
                    ************************************************************

HsCD00585861        TTAAGAGCATTCATCAGAAAACAAATTAACAACATTTTCCTCAGGTTTATATATGAAACA
NM_001199756.1      TTAAGAGCATTCATCAGAAAACAAATTAACAACATTTTCCTCAGGTTTATATATGAAACA
NM_006243.3         TTAAGAGCATTCATCAGAAAACAAATTAACAACATTTTCCTCAGGTTTATATATGAAACA
                    ************************************************************

HsCD00585861        GAACATTTCAATGGTGTTGCTGAACTTCTTGAAATATTAGGAAGTATTACCAATGGCTTT
NM_001199756.1      GAACATTTCAATGGTGTTGCTGAACTTCTTGAAATATTAGGAAGTATTATCAATGGCTTT
NM_006243.3         GAACATTTCAATGGTGTTGCTGAACTTCTTGAAATATTAGGAAGTATTATCAATGGCTTT
                    ************************************************* **********

HsCD00585861        GCATTGCCACTGAAAGCAGAACATAAACAATTTCTAATGAAGGTTCTTATTCCTATGCAT
NM_001199756.1      GCATTGCCACTGAAAGCAGAACATAAACAATTTCTAATGAAGGTTCTTATTCCTATGCAT
NM_006243.3         GCATTGCCACTGAAAGCAGAACATAAACAATTTCTAATGAAGGTTCTTATTCCTATGCAT
                    ************************************************************

HsCD00585861        ACTGCAAAAGGATTAGCTTTGTTTCATGCTCAGCTAGCATATTGTGTTGTACAGTTCCTG
NM_001199756.1      ACTGCAAAAGGATTAGCTTTGTTTCATGCTCAGCTAGCATATTGTGTTGTACAGTTCCTG
NM_006243.3         ACTGCAAAAGGATTAGCTTTGTTTCATGCTCAGCTAGCATATTGTGTTGTACAGTTCCTG
                    ************************************************************

HsCD00585861        GAGAAAGATACAACACTAACAGAGCCAGTGATCAGAGGACTGCTGAAATTTTGGTCAAAA
NM_001199756.1      GAGAAAGATACAACACTAACAGAGCCAGTGATCAGAGGACTGCTGAAATTTTGGCCAAAA
NM_006243.3         GAGAAAGATACAACACTAACAGAGCCAGTGATCAGAGGACTGCTGAAATTTTGGCCAAAA
                    ****************************************************** *****

HsCD00585861        ACCTGCAGTCAGAAAGAGGTGATGTTTTTAGGAGAAATTGAAGAAATCTTAGATGTCATT
NM_001199756.1      ACCTGCAGTCAGAAAGAGGTGATGTTTTTAGGAGAAATTGAAGAAATCTTAGATGTCATT
NM_006243.3         ACCTGCAGTCAGAAAGAGGTGATGTTTTTAGGAGAAATTGAAGAAATCTTAGATGTCATT
                    ************************************************************

HsCD00585861        GAACCAACACAGTTCAAAAAAATTGAAGAGCCACTTTTCAAGCAGATATCCAAGTGTGTA
NM_001199756.1      GAACCAACACAGTTCAAAAAAATTGAAGAGCCACTTTTCAAGCAGATATCCAAGTGTGTA
NM_006243.3         GAACCAACACAGTTCAAAAAAATTGAAGAGCCACTTTTCAAGCAGATATCCAAGTGTGTA
                    ************************************************************

HsCD00585861        TCCAGTTCTCGTTTTCAGGTTGCAGAAAGGGCATTGTACTTCTGGAATAACGAATATATT
NM_001199756.1      TCCAGTTCTCATTTTCAGGTTGCAGAAAGGGCATTGTACTTCTGGAATAACGAATATATT
NM_006243.3         TCCAGTTCTCATTTTCAGGTTGCAGAAAGGGCATTGTACTTCTGGAATAACGAATATATT
                    **********.*************************************************

HsCD00585861        CTTAGTTTGATTGAGGAGAACATTGATAAAATTCTGCCAATTATGTTTTGCCTAG-----
NM_001199756.1      CTTAGTTTGATTGAGGAGAACATTGATAAAATTCTGCCAATTATGTTTGCCAGTTTGTAC
NM_006243.3         CTTAGTTTGATTGAGGAGAACATTGATAAAATTCTGCCAATTATGTTTGCCAGTTTGTAC
                    ************************************************  *. :      

HsCD00585861        ------------------------------------------------------------
NM_001199756.1      AAAATTTCCAAAGAACACTGGAATCCGACCATTGTAGCACTGGTATACAATGTGCTGAAA
NM_006243.3         AAAATTTCCAAAGAACACTGGAATCCGACCATTGTAGCACTGGTATACAATGTGCTGAAA
                                                                                

HsCD00585861        ------------------------------------------------------------
NM_001199756.1      ACCCTAATGGAAATGAATGGCAAGCTTTTCGATGACCTTACTAGCTCATACAAAGCTGAA
NM_006243.3         ACCCTAATGGAAATGAATGGCAAGCTTTTCGATGACCTTACTAGCTCATACAAAGCTGAA
                                                                                

HsCD00585861        ------------------------------------------------------------
NM_001199756.1      AGACAGAGAGAGAAAAAGAAGGAATTGGAACGTGAAGAATTATGGAAAAAATTAGAGGAG
NM_006243.3         AGACAGAGAGAGAAAAAGAAGGAATTGGAACGTGAAGAATTATGGAAAAAATTAGAGGAG
                                                                                

HsCD00585861        ------------------------------------------------------------
NM_001199756.1      CTAAAGCTAAAGAAAGCTCTAGAAAAACAGAATAGTGCTTACAACATGCACAGTATTCTC
NM_006243.3         CTAAAGCTAAAGAAAGCTCTAGAAAAACAGAATAGTGCTTACAACATGCACAGTATTCTC
                                                                                

HsCD00585861        ---------------------
NM_001199756.1      AGCAATACAAGTGCCGAATAA
NM_006243.3         AGCAATACAAGTGCCGAATAA

Pathways

NCI :

C-MYC pathway

NCI :

Canonical Wnt signaling pathway

NCI :

Validated transcriptional targets of deltaNp63 isoforms

Panther :

EGF receptor signaling pathway

Panther :

FGF signaling pathway

Panther :

Wnt signaling pathway

Reactome :

Adaptive Immune System

Reactome :

Beta-catenin phosphorylation cascade

Reactome :

Cell Cycle

Reactome :

Cell Cycle, Mitotic

Reactome :

Costimulation by the CD28 family

Reactome :

CTLA4 inhibitory signaling

Reactome :

Degradation of beta-catenin by the destruction complex

Reactome :

disassembly of the destruction complex and recruitment of AXIN to the membrane

Reactome :

Hemostasis

Reactome :

Immune System

Reactome :

M Phase

Reactome :

Mitotic Anaphase

Reactome :

Mitotic M-M/G1 phases

Reactome :

Mitotic Metaphase and Anaphase

Reactome :

Mitotic Prometaphase

Reactome :

Platelet homeostasis

Reactome :

Platelet sensitization by LDL

Reactome :

Resolution of Sister Chromatid Cohesion

Reactome :

Separation of Sister Chromatids

Reactome :

Signal Transduction

Reactome :

Signaling by Wnt

Reactome :

TCF dependent signaling in response to WNT

WikiPathway :

Glycogen Metabolism

Protein

Structural Annotation Wiki :

HR3092

UniProt :

Q15172

HPRD :

03384

Clone

Cloning Information :

628586

Original Clone ID :

HR3092-19-397-14.9

Experimental

TargetTrack - Experimental data :

HR3092

Organism

Species Specific ID:

5525

Genome

Chromosome :

Map Location :

1q32.2-q32.3

Ensembl :

ENSG00000066027

Reagents

Labome :

PPP2R5A-antibody

PPP2R5A (H. sapiens )In pET15_NESG (His-tagged bacterial expression vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Reference Sequence Alignment

Pathways

Protein

Clone

Experimental

Organism

Genome

Reagents

PPP2R5A (H. sapiens )
In pET15_NESG (His-tagged bacterial expression vector)