Gene:
Gene Symbol:
Dtox_3130
Gene Name:
radical SAM protein
Original Clone ID:
APC100450.106
Keyword:
None
Species:
Desulfofarcimen acetoxidans DSM 771
Type:
cDNA
Vector Name:
pMCSG73
Format:
FUSION
Source:
Midwest Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Argonne National Laboratory
Michael Endres
PSI:Biology
Midwest Center for Structural Genomics
Michael Endres
PSI:Biology
Midwest Center for Structural Genomics
DaCD00586741
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 1212nts
Open reading frame: 5 to 1216
TGCCGAAGATGAAAACGTCGGAAAGTTGAATTTAGAGGACAAGCCTGATTTGGTTGGAAT
TAGCGTAAACGTGGACACATCACAACGAGCCTACGAAATCTCAAAACATTACCGCAAGCA
GGGAATTAAGGTTATTTTAGGCGGGATTCACGCTAGTGCCAATTCGGATGAGGCCCTAGC
TTACGCTGACTCAGTTTGCATTGGAGAAGCGGAGGAGTTATGGGAACAAGTTATAGCAGA
TGTCCAAACAGGCAATTTAAAAAAGAAATATTATAATGATAAGCCCACTAATCTAGCCAA
TACTCCTATTCCCAAATGGAGTTTAATCGACAAATCTAAGTATTTGTACACTAACGTTGT
GGTAACTAGCAGGGGATGTCCCTTTCGATGTGAGTTTTGTTATAACAGTTGTGAATACGT
GCATAACAAGTTTAGAAATCGCCCCATTGAACACGTTATTGAAGAAGTCAAAGCTTTAGG
TACTAAGCAGGTCATGTTTATTGACGACAATTTTATTGGAAATATTATCTGGACAAAGGA
ATTCCTAAGGCAAATTAAGCCACTTTATCTGAAATGGCATGCAGCAGTTTCAGCTAACTT
AGTTCAGCACCTCGATCTTTTAGATGAAATGAAGGAGTCAGGATGTGAAAGTCTATTTAT
TGGCTTTGAGACGATTAATAAAGCTTCCATTGAAAGTGTAAGTAAATACCAAAACAATAT
TGAGAGTTATGAAAAGCTAATCCAGGAGATACATTCTAGAGGCATAATGGTCAATGCCAG
CTTGGTATTTGGTTTTGATGATGATGAAGATACCGTATTTGAGGAGACGTTGAATTGGCT
TGTTAAAAATAAAATAGAGACAATGACAGCCCATATTTTAACTCCGTACCCGGGAACAAA
GATCTATCAAAGATTGAAATCCCAAAGCCGCATTATTGATCATGAACCAACCCATTATAA
CACTTCTTATGTAGTTTTCTCACCCAATAAAATGACCCAGCAACAACTTTATGAAGGTTA
TCTGAAAATATATGAGGACTTCTATTCAATTAAAAATATCATAAAAAGACTTCCCAATGA
TCGGAACCAAAGGATTCCTTATTTATTATTTAATTTTGTCTATCGTAAATTCGGAAAGAT
AACATCAAAAATTGCGAGCTTTGGCTTGATGAATAATTTAGGCAAATCAGCGCGAAAATT
GTCCTATGGTATTGAT
Protein sequence predicted based on nucleotide sequence
EDENVGKLNLEDKPDLVGISVNVDTSQRAYEISKHYRKQGIKVILGGIHASANSDEALAY ADSVCIGEAEELWEQVIADVQTGNLKKKYYNDKPTNLANTPIPKWSLIDKSKYLYTNVVV TSRGCPFRCEFCYNSCEYVHNKFRNRPIEHVIEEVKALGTKQVMFIDDNFIGNIIWTKEF LRQIKPLYLKWHAAVSANLVQHLDLLDEMKESGCESLFIGFETINKASIESVSKYQNNIE SYEKLIQEIHSRGIMVNASLVFGFDDDEDTVFEETLNWLVKNKIETMTAHILTPYPGTKI YQRLKSQSRIIDHEPTHYNTSYVVFSPNKMTQQQLYEGYLKIYEDFYSIKNIIKRLPNDR NQRIPYLLFNFVYRKFGKITSKIASFGLMNNLGKSARKLSYGID
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in MCSG-APC100450
Recommended expression in:
Not Applicable
Vector Name:
pMCSG73
Synonyms:
''
Sequencing Primer:
Forward:
MCSG73F
Reverse:
T7 terminator
Description:
Bacterial expression vector with a T7 promoter and TVMV-cleavable N-terminal NusA tag and TEV-cleavable N-terminal 6xHis and StrepII tag; Contains rate argU and ileX tRNA genes; ampicillin resistance in bacteria; ligation independent cloning.
Comments:
None
Size (bp):
6227
Parent Vector:
Name:
pMCSG53
Description:
Properties:
bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name:
Midwest Center for Structural Genomics
PSI:Biology
Midwest Center for Structural Genomics
PSI:Biology
Midwest Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
pBR322 origin
pBR322 origin of replication
4220
4808
ligation independent cloning
LIC
ligation independent cloning site
138
16
promoter
T7
T7 promoter
1779
1795
protease cleavage site
TVMV
TVMV protease cleavage site
218
238
protease cleavage site
TEV
TEV protease cleavage site
155
175
repressor binding site
lac operator
lac operator
1754
1776
repressor protein gene
LacI
LacI CDS
2832
3791
selectable marker
AmpR
ampicillin resistance gene
4979
5839
tRNA
ileX
ileX tRNA gene
2378
2529
tRNA
argU
argU tRNA
1862
2218
tag
NusA
N-terminal NusA
249
1708
tag
His
N-terminal 6xHis tag
200
217
tag
strep
N-terminal StrepII tag
176
199
trxn termination sequence
T7 term
T7 terminator
26
72
