DNASU Plasmid | VTA1 (H. sapiens )<br>In pGEX2T(TEV) (GST-tagged bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: VTA1

Gene Name: vesicle (multivesicular body) trafficking 1

Original Clone ID:

WISP10-492

Keyword:

LIP5 aa 1-168, M64A

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pGEX2T(TEV)

Format:

CLOSED

Source:

University of Utah

Description:

None

Comments:

None

Mutation / Discrepancy:

Yes / No

Publications:

None

Authors:

University of Utah
William Stubblefield
Wesley I. Sundquist

HsCD00587777

Price:

AVAILABLE

No restriction
Special MTA: SPTA

Insert Sequence
Protein Sequence

Insert sequence: 507nts

Open reading frame: 1 to 507

Reference Sequence Alignment

ATGGCCGCGCTTGCACCGCTGCCCCCGCTCCCCGCACAGTTCAAGAGCATACAGCATCAT
CTGAGGACGGCTCAGGAGCATGACAAGCGAGACCCTGTGGTGGCTTATTACTGTCGTTTA
TACGCAATGCAGACTGGAATGAAGATCGATAGTAAAACTCCTGAATGTCGCAAATTTTTA
TCAAAGTTAGCGGATCAGTTAGAAGCTCTAAAGAAGCAGTTGGGTGATAATGAAGCTATT
ACTCAAGAAATAGTGGGCTGTGCCCATTTGGAGAATTATGCTTTGAAAATGTTTTTGTAT
GCAGACAATGAAGATCGTGCTGGACGATTTCACAAAAACATGATCAAGTCCTTCTATACT
GCAAGTCTTTTGATAGATGTCATAACAGTATTTGGAGAACTCACTGATGAAAATGTGAAA
CACAGGAAGTATGCCAGATGGAAGGCAACATACATCCATAATTGTTTAAAGAATGGGGAG
ACTCCTCAAGCAGGCCCTGTTGGTTAG

Protein sequence predicted based on nucleotide sequence

MAALAPLPPLPAQFKSIQHHLRTAQEHDKRDPVVAYYCRLYAMQTGMKIDSKTPECRKFL
SKLADQLEALKKQLGDNEAITQEIVGCAHLENYALKMFLYADNEDRAGRFHKNMIKSFYT
ASLLIDVITVFGELTDENVKHRKYARWKATYIHNCLKNGETPQAGPVG*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

End read sequencing of cDNA insert

Mutations:

M64A

a190g; t191c

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pGEX2T(TEV)

Synonyms:

Sequencing Primer:

Forward: pGEX5

Reverse: pGEX3

Description:

Bacterial Expression vector with an N-terminal GST tag and TEV site; ampicillin resistance in bacteria; restriction enzyme cloning.

Comments:

Sundquist Lab internal database number = WISP 01-69; Nde1, BamH1 restriction enzyme cloning can be used easily with this vector

Size (bp):

4993

Parent Vector:

None

Properties:

bacterial expression, with tag/fusion/marker

Author Name:

University of Utah
Wesley I. Sundquist

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

Not Available

Vector Features:

Type

Name

Description

Start

End

primer

forward primer

pGEX forward sequencing primer GGTTCCGCGTGGATCTCCGG

920

939

primer

reverse primer

pGEX reverse sequencing primer CCGGGAGCTGCATGTGTCAGAGG

1043

1065

promoter

tac promoter

180

209

protease cleavage site

TEV

N-terminal TEV protease cleavage site

reporter gene

LacZ

LacZ alpha

4655

repressor binding site

lac operator

lac operator binding site

215

238

selectable marker

AmpR

Ampicillin resistance

1599

2259

tag

GST

N-terminal GST tag

258

929

Gene

Gene Symbol :

VTA1

Symbol Nomenclature :

VTA1

SYNONYM :

C6orf55; DRG-1; DRG1; LIP5; My012; SBP1

Designation :

Full Nomenclature :

vesicle (multivesicular body) trafficking 1

GENEID :

51534

Locus Tag :

HSPC228

GenBank Accession :

BC022536

HGNC :

20954

MIM :

610902

Target GenBank:

BC022536

Reference Sequence Alignment

  

HsCD00587777        ------------------------------------------------------------
NM_016485.4         ATGGCCGCGCTTGCACCGCTGCCCCCGCTCCCCGCACAGTTCAAGAGCATACAGCATCAT
NM_001286371.1      ATGGCCGCGCTTGCACCGCTGCCCCCGCTCCCCGCACAGTTCAAGAGCATACAGCATCAT
NM_001286372.1      ------------------------------------------------------------
                                                                                

HsCD00587777        ------------------------------------------------------------
NM_016485.4         CTGAGGACGGCTCAGGAGCATGACAAGCGAGACCCTGTGGTGGCTTATTACTGTCGTTTA
NM_001286371.1      CTGAGGACGGCTCAGGAGCATGACAAGCGAGACCCTGTGGTGGCTTATTACTGTCGTTTA
NM_001286372.1      -------------------ATGACAAGCGAGACCCTGTGGTGGCTTATTACT--------
                                                                                

HsCD00587777        ------------------------------------------------------------
NM_016485.4         TACGCAATGCAGACTGGAATGAAGATCGATAGTAAAACTCCTGAATGTCGCAAATTTTTA
NM_001286371.1      TACGCAATGCAGACTGGAATGAAGATCGATAGTAAAACTCCTGAATGTCGCAAATTTTTA
NM_001286372.1      ------------------------------------------------------------
                                                                                

HsCD00587777        ------------------------------------------------------------
NM_016485.4         TCAAAGTTAATGGATCAGTTAGAAGCTCTAAAGAAGCAGTTGGGTGATAATGAAGCTATT
NM_001286371.1      TCAAAGTTAATGGATCAGTTAGAAGCTCTAAAGAAGCAGTTGGGTGATAATGAAGCTATT
NM_001286372.1      ---------------------------CTAAAGAAGCAGTTGGGTGATAATGAAGCTATT
                                                                                

HsCD00587777        ------------------------------------------------ATGGCCGCGCTT
NM_016485.4         ACTCAAGAAATAGTGGGCTGTGCCCATTTGGAGAATTATGCTTTGAAAATGTTTTTGTAT
NM_001286371.1      ACTCAAGAAATAGTGGGCTGTGCCCATTTGGAGAATTATGCTTTGAAAATGTTTTTGTAT
NM_001286372.1      ACTCAAGAAATAGTGGGCTGTGCCCATTTGGAGAATTATGCTTTGAAAATGTTTTTGTAT
                                                                    ***     * :*

HsCD00587777        GCACCGCTGCCCCCGCTCCCCGCACAGTTCAAGAGCATACAGCATCATCTGAGGACGGCT
NM_016485.4         GCAGACAATGAAGATCGTGCTGGACGATTTCACAAAAACATGATCAAGTCCTTCTATACT
NM_001286371.1      GCAGACAATGAAGATCGTGCTGGACGATTTCACAAAAACATGATCAAGTCCTTCTATACT
NM_001286372.1      GCAGACAATGAAGATCGTGCTGGACGATTTCACAAAAACATGATCAAGTCCTTCTATACT
                    *** . .:  .. . *   * * **..** .* *..*:..:*.: .*    :  :. .**

HsCD00587777        CAGGAGCATGACAAGCGAGACCCTGTGGTGGCTTATTACTGTCGTTTATACGCAATGCAG
NM_016485.4         GCAAGTCTTTTGATAGATGTCATAACAGTATTTGGAGAACTCACTGATGAAAATGTGAAA
NM_001286371.1      GCAAGTCTTTTGATAGATGTCATAACAGTATTTGGAGAACTCACTGATGAAAATGTGAAA
NM_001286372.1      GCAAGTCTTTTGATAGATGTCATAACAGTATTTGGAGAACTCACTGATGAAAATGTGAAA
                     .... *:* : *:. .:*:*. :. .**.  * .: *.   . * :: *...:.**.*.

HsCD00587777        ACTGGAATGAAGATCGATAGTAAAACTCCTGAATGTCGCAAATTTTTATCAAAGTTAGCG
NM_016485.4         CACAGGAAGTATGCCAGATGGAAGGCAACATACATCCATAATTGTTTAAAGAATGGGGAG
NM_001286371.1      CACAGGAAGTATGCCAGATGGAAGGCAACATACATCCATAATTGTTTAAAGAATGGGGAG
NM_001286372.1      CACAGGAAGTATGCCAGATGGAAGGCAACATACATCCATAATTGTTTAAAGAATGGGGAG
                    .. .*.*:*:* . *..::* **..*:.*: *.:  *. **:* ****:..**   .*.*

HsCD00587777        GATCAGTTAGAAGCTCTAAAGAAGCAGTTGGGTGATAATGAAGCTATTACTCAAGAAATA
NM_016485.4         ACTCCTCAAGCAGGCCCTGTTGGAATTGAAGAAGATAATGATATTGAAGAAAATGAAGAT
NM_001286371.1      ACTCCTCAAGCAGGCCCTGTTGGAATTGAAGAAGATAATGATATTGAAGAAAATGAAGAT
NM_001286372.1      ACTCCTCAAGCAGGCCCTGTTGGAATTGAAGAAGATAATGATATTGAAGAAAATGAAGAT
                    ..**.  :**.**  * :.: ....:  :.*.:********:. *.::..:.*:***.::

HsCD00587777        GTGGGCTGTGCCCATTTGGAGAATTATGCTTTGAAAATGTTTTTGTATGCAGACAATGAA
NM_016485.4         GCTGGAGCAGCCTCTCTGCCCACTCAGCCAACTCAGCCATCATCATCTTCAACTTATGAC
NM_001286371.1      GCTGGAGCAGCCTCTCTGCCCACTCAGCCAACTCAGCCATCATCATCTTCAACTTATGAC
NM_001286372.1      GCTGGAGCAGCCTCTCTGCCCACTCAGCCAACTCAGCCATCATCATCTTCAACTTATGAC
                    *  **.  :*** .* ** . *.* *  *::  .*.. .* :* .*.* **.. :****.

HsCD00587777        GATCGTGCTGGACGATTTCACAAAAACATGATCAAGTCCTTCTATACTGCAAGTCTTTTG
NM_016485.4         CCAAGCAACATGCCATCAGGCAACTATACTGGAATACAGATTCCTCCGGGTGCACACGCT
NM_001286371.1      CCAAGCAACATGCCATCAGGCAACTATACTGGAATACAGATTCCTCCGGGTGCACACGCT
NM_001286372.1      CCAAGCAACATGCCATCAGGCAACTATACTGGAATACAGATTCCTCCGGGTGCACACGCT
                     .:.* .. . .* ** : .***.:* *  . .*:. . :*  .*.* * :. :*:    

HsCD00587777        ATAGATGTCATAACAGTATTTGG-------------------------------------
NM_016485.4         CCAGCTAATACACCAGCAGAAGTGCCTCACAGCACAGGTGTAGCAAGTAATACTATCCAA
NM_001286371.1      CCAGCTAATACACCAGCAGAAGTGCCTCACAGCACAG-----------------------
NM_001286372.1      CCAGCTAATACACCAGCAGAAGTGCCTCACAGCACAG-----------------------
                    . **.*.: * *.*** * ::*                                      

HsCD00587777        ------------------------------------------------------------
NM_016485.4         CCTACTCCACAGACTATACCTGCCATTGATCCCGCACTTTTCAATACAATTTCCCAGGGG
NM_001286371.1      ----------------------------------------------------------GG
NM_001286372.1      ----------------------------------------------------------GG
                                                                                

HsCD00587777        ------------------------------AGAACTCACTGATGAAAATGTGAAACACAG
NM_016485.4         GATGTTCGTCTAACCCCAGAAGACTTTGCTAGAGCTCAGAAGTACTGCAAATATGCTGGC
NM_001286371.1      GATGTTCGTCTAACCCCAGAAGACTTTGCTAGAGCTCAGAAGTACTGCAAATATGCTGGC
NM_001286372.1      GATGTTCGTCTAACCCCAGAAGACTTTGCTAGAGCTCAGAAGTACTGCAAATATGCTGGC
                                                  ***.**** :..*..:..:.: *:.*: . 

HsCD00587777        GAAGTATGCCAGATGGAAGGCAACATACATCCATAATTGTTTAAAGAATGGGGAGACTCC
NM_016485.4         AGTGCTTTGCAGTATGAAGATGTAAGCACTGCTGTCCAGAATCTACAAAAGGCTCTCAAG
NM_001286371.1      AGTGCTTTGCAGTATGAAGATGTAAGCACTGCTGTCCAGAATCTACAAAAGGCTCTCAAG
NM_001286372.1      AGTGCTTTGCAGTATGAAGATGTAAGCACTGCTGTCCAGAATCTACAAAAGGCTCTCAAG
                    ..:* :*  ***:: ****. .:.* ...* *: :. :*::*.:* **:.** : :*:. 

HsCD00587777        TCAAGCAGGCCCTGTTGGTTAG--
NM_016485.4         TTACTGACGACAGGCAGAGAATGA
NM_001286371.1      TTACTGACGACAGGCAGAGAATGA
NM_001286372.1      TTACTGACGACAGGCAGAGAATGA
                    * *.  * *.*. * :*. :*

Pathways

Reactome :

Budding and maturation of HIV virion

Reactome :

Disease

Reactome :

Endosomal Sorting Complex Required For Transport (ESCRT)

Reactome :

HIV Infection

Reactome :

HIV Life Cycle

Reactome :

Late Phase of HIV Life Cycle

Reactome :

Membrane Trafficking

Protein

HPRD :

09855

Clone

Cloning Information :

621311

Original Clone ID :

WISP10-492

Organism

Species Specific ID:

51534

Genome

Chromosome :

Map Location :

6q24.1

Reagents

Labome :

VTA1-antibody

VTA1 (H. sapiens )In pGEX2T(TEV) (GST-tagged bacterial expression vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Reference Sequence Alignment

Pathways

Protein

Clone

Organism

Genome

Reagents

VTA1 (H. sapiens )
In pGEX2T(TEV) (GST-tagged bacterial expression vector)