Gene:
Gene Symbol:
VTA1
Gene Name:
vesicle (multivesicular body) trafficking 1
Original Clone ID:
WISP08-36
Keyword:
LIP5 aa 1-307
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pCAG.Myc
Format:
FUSION
Source:
University of Utah
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Utah
Eiji Morita
Wesley I. Sundquist
Eiji Morita
Wesley I. Sundquist
HsCD00587779
Price:
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AVAILABLE
No restriction
Special MTA: SPTA
Special MTA: SPTA
ATGGCCGCGCTTGCACCGCTGCCCCCGCTCCCCGCACAGTTCAAGAGCATACAGCATCAT
CTGAGGACGGCTCAGGAGCATGACAAGCGAGACCCTGTGGTGGCTTATTACTGTCGTTTA
TACGCAATGCAGACTGGAATGAAGATCGATAGTAAAACTCCTGAATGTCGCAAATTTTTA
TCAAAGTTAATGGATCAGTTAGAAGCTCTAAAGAAGCAGTTGGGTGATAATGAAGCTATT
ACTCAAGAAATAGTGGGCTGTGCCCATTTGGAGAATTATGCTTTGAAAATGTTTTTGTAT
GCAGACAATGAAGATCGTGCTGGACGATTTCACAAAAACATGATCAAGTCCTTCTATACT
GCAAGTCTTTTGATAGATGTCATAACAGTATTTGGAGAACTCACTGATGAAAATGTGAAA
CACAGGAAGTATGCCAGATGGAAGGCAACATACATCCATAATTGTTTAAAGAATGGGGAG
ACTCCTCAAGCAGGCCCTGTTGGAATTGAAGAAGATAATGATATTGAAGAAAATGAAGAT
GCTGGAGCAGCCTCTCTGCCCACTCAGCCAACTCAGCCATCATCATCTTCAACTTATGAC
CCAAGCAACATGCCATCAGGCAACTATACTGGAATACAGATTCCTCCGGGTGCACACGCT
CCAGCTAATACACCAGCAGAAGTGCCTCACAGCACAGGTGTAGCAAGTAATACTATCCAA
CCTACTCCACAGACTATACCTGCCATTGATCCCGCACTTTTCAATACAATTTCCCAGGGG
GATGTTCGTCTAACCCCAGAAGACTTTGCTAGAGCTCAGAAGTACTGCAAATATGCTGGC
AGTGCTTTGCAGTATGAAGATGTAAGCACTGCTGTCCAGAATCTACAAAAGGCTCTCAAG
TTACTGACGACAGGCAGAGAATGA
Protein sequence predicted based on nucleotide sequence
MAALAPLPPLPAQFKSIQHHLRTAQEHDKRDPVVAYYCRLYAMQTGMKIDSKTPECRKFL SKLMDQLEALKKQLGDNEAITQEIVGCAHLENYALKMFLYADNEDRAGRFHKNMIKSFYT ASLLIDVITVFGELTDENVKHRKYARWKATYIHNCLKNGETPQAGPVGIEEDNDIEENED AGAASLPTQPTQPSSSSTYDPSNMPSGNYTGIQIPPGAHAPANTPAEVPHSTGVASNTIQ PTPQTIPAIDPALFNTISQGDVRLTPEDFARAQKYCKYAGSALQYEDVSTAVQNLQKALK LLTTGRE*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
End read sequencing of cDNA insert
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pCAG.Myc
Synonyms:
''
Sequencing Primer:
Forward:
Unknown
Reverse:
Unknown
Description:
Mammalian expression vector with a N-terminal myc tag; ampicillin resistance in bacteria; restriction enzyme cloning.
Comments:
Sundquist Lab internal database number = WISP 06-64; Restriction enzyme cloning can be used with this vector - e.g. Xho1, Kpn1 works well
Size (bp):
4899
Parent Vector:
None
Properties:
mammalian expression, multiple cloning site, with tag/fusion/marker
Author Name:
University of Utah
Wesley I. Sundquist
Wesley I. Sundquist
Publications:
PMID:
19525971
Title: Structural basis for ESCRT-III protein autoinhibition.
Vector Map:
Vector Sequence:
Sequence:
Not Available
Vector Features:
Type
Name
Description
Start
End
primer
forward primer
CAGF forward sequencing primer TCGGCTTCTGGCGTGTGACC
1606
1625
primer
reverse primer
CAGR reverse sequencing primer
1929
1952
promoter
CBA
CBA promoter
0
0
selectable marker
AmpR
ampicillin resistance
3911
4570
tag
c-myc
N-terminal c-myc tag
0
0
