DNASU Plasmid | CHMP5 (H. sapiens )<br>In pCAG.MCS2.C-Myc (Myc-tagged mammalian expression vector)

Explanation of Terms

Gene:

Gene Symbol: CHMP5

Gene Name: charged multivesicular body protein 5

Original Clone ID:

WISP11-322

Keyword:

CHMP5 aa 1-219, L163D, L167D, L170D, L174D

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pCAG.MCS2.C-Myc

Format:

FUSION

Source:

University of Utah

Description:

None

Comments:

None

Mutation / Discrepancy:

Yes / No

Publications:

None

Authors:

University of Utah
Angela Katsuyama
Wesley I. Sundquist

HsCD00587799

Price:

AVAILABLE

No restriction
Special MTA: SPTA

Insert Sequence
Protein Sequence

Insert sequence: 657nts

Open reading frame: 1 to 657

Reference Sequence Alignment

ATGAACCGACTCTTCGGGAAAGCGAAACCCAAGGCTCCGCCGCCCAGCCTGACTGACTGC
ATTGGCACGGTGGACAGTAGAGCAGAATCCATTGACAAGAAGATTTCTCGATTGGATGCT
GAGCTAGTGAAGTATAAGGATCAGATCAAGAAGATGAGAGAGGGTCCTGCAAAGAATATG
GTCAAGCAGAAAGCCTTGCGAGTTTTAAAGCAAAAGAGGATGTATGAGCAGCAGCGGGAC
AATCTTGCCCAACAGTCATTCAACATGGAACAAGCCAATTATACCATCCAGTCTTTGAAG
GACACCAAGACCACGGTTGATGCTATGAAACTGGGAGTAAAGGAAATGAAGAAGGCATAC
AAGCAAGTGAAGATCGACCAGATTGAGGATTTACAAGACCAGCTAGAGGATATGATGGAA
GATGCAAATGAAATCCAAGAAGCACTGAGTCGCAGTTATGGCACCCCAGAACTGGATGAA
GATGATGACGAAGCAGAGGACGATGCAGACGGTGATGAGGACCTGGCTGATGAAGACAGT
TCTTATTTGGATGAGGCAGCATCTGCACCTGCAATTCCAGAAGGTGTTCCCACTGATACA
AAAAACAAGGATGGAGTTCTGGTGGATGAATTTGGATTGCCACAGATCCCTGCTTCA

Protein sequence predicted based on nucleotide sequence

MNRLFGKAKPKAPPPSLTDCIGTVDSRAESIDKKISRLDAELVKYKDQIKKMREGPAKNM
VKQKALRVLKQKRMYEQQRDNLAQQSFNMEQANYTIQSLKDTKTTVDAMKLGVKEMKKAY
KQVKIDQIEDLQDQLEDMMEDANEIQEALSRSYGTPELDEDDDEAEDDADGDEDLADEDS
SYLDEAASAPAIPEGVPTDTKNKDGVLVDEFGLPQIPAS

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

End read sequencing of cDNA insert

Mutations:

L163D, L167D, L170D, L174D

t487g; t488a; a489c; t499g; t500a; g501c; c508g; t509a; a510c; c520g; t521a; t522c

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: chloramphenicol, ampicillin

Growth Condition: LB with 35ug/ml chloramphenicol and 100ug/ml ampicillin

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pCAG.MCS2.C-Myc

Synonyms:

Sequencing Primer:

Forward: Unknown

Reverse: Unknown

Description:

Mammalian expression vector with a C-terminal myc tag; ampicillin resistance in bacteria; restriction enzyme cloning.

Comments:

Sundquist Lab internal database number = WISP 08-104; Restriction enzyme cloning can be used with this vector - e.g. Xho1, Kpn1 works well

Size (bp):

4813

Parent Vector:

None

Properties:

mammalian expression, multiple cloning site, with tag/fusion/marker

Author Name:

University of Utah
Wesley I. Sundquist

Publications:

PMID: 19525971

Title: Structural basis for ESCRT-III protein autoinhibition.

Vector Map:

Vector Sequence:

Sequence:

Not Available

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ColE

ColE1 origin

3045

3727

primer

forward primer

CAGF forward sequencing primer

1605

1624

primer

reverse primer

CAGR reverse sequencing primer TGTCCCCATAATTTTTGGCAGAGG

1845

1868

selectable marker

AmpR

Ampicillin resistance

3824

4484

tag

c-myc

C-terminal may tag

Gene

Gene Symbol :

CHMP5

Symbol Nomenclature :

CHMP5

SYNONYM :

C9orf83; HSPC177; PNAS-2; SNF7DC2; Vps60

Designation :

Full Nomenclature :

charged multivesicular body protein 5

GENEID :

51510

Locus Tag :

CGI-34

GenBank Accession :

BC007457

HGNC :

26942

MIM :

610900

Target GenBank:

BC007457

Reference Sequence Alignment

  

HsCD00587799      ATGAACCGACTCTTCGGGAAAGCGAAACCCAAGGCTCCGCCGCCCAGCCTGACTGACTGC
NM_016410.5       ATGAACCGACTCTTCGGGAAAGCGAAACCCAAGGCTCCGCCGCCCAGCCTGACTGACTGC
                  ************************************************************

HsCD00587799      ATTGGCACGGTGGACAGTAGAGCAGAATCCATTGACAAGAAGATTTCTCGATTGGATGCT
NM_016410.5       ATTGGCACGGTGGACAGTAGAGCAGAATCCATTGACAAGAAGATTTCTCGATTGGATGCT
                  ************************************************************

HsCD00587799      GAGCTAGTGAAGTATAAGGATCAGATCAAGAAGATGAGAGAGGGTCCTGCAAAGAATATG
NM_016410.5       GAGCTAGTGAAGTATAAGGATCAGATCAAGAAGATGAGAGAGGGTCCTGCAAAGAATATG
                  ************************************************************

HsCD00587799      GTCAAGCAGAAAGCCTTGCGAGTTTTAAAGCAAAAGAGGATGTATGAGCAGCAGCGGGAC
NM_016410.5       GTCAAGCAGAAAGCCTTGCGAGTTTTAAAGCAAAAGAGGATGTATGAGCAGCAGCGGGAC
                  ************************************************************

HsCD00587799      AATCTTGCCCAACAGTCATTCAACATGGAACAAGCCAATTATACCATCCAGTCTTTGAAG
NM_016410.5       AATCTTGCCCAACAGTCATTCAACATGGAACAAGCCAATTATACCATCCAGTCTTTGAAG
                  ************************************************************

HsCD00587799      GACACCAAGACCACGGTTGATGCTATGAAACTGGGAGTAAAGGAAATGAAGAAGGCATAC
NM_016410.5       GACACCAAGACCACGGTTGATGCTATGAAACTGGGAGTAAAGGAAATGAAGAAGGCATAC
                  ************************************************************

HsCD00587799      AAGCAAGTGAAGATCGACCAGATTGAGGATTTACAAGACCAGCTAGAGGATATGATGGAA
NM_016410.5       AAGCAAGTGAAGATCGACCAGATTGAGGATTTACAAGACCAGCTAGAGGATATGATGGAA
                  ************************************************************

HsCD00587799      GATGCAAATGAAATCCAAGAAGCACTGAGTCGCAGTTATGGCACCCCAGAACTGGATGAA
NM_016410.5       GATGCAAATGAAATCCAAGAAGCACTGAGTCGCAGTTATGGCACCCCAGAACTGGATGAA
                  ************************************************************

HsCD00587799      GATGATGACGAAGCAGAGGACGATGCAGACGGTGATGAGGACCTGGCTGATGAAGACAGT
NM_016410.5       GATGATTTAGAAGCAGAGTTGGATGCACTAGGTGATGAGCTTCTGGCTGATGAAGACAGT
                  ****** :.********* : ****** :.********* : ******************

HsCD00587799      TCTTATTTGGATGAGGCAGCATCTGCACCTGCAATTCCAGAAGGTGTTCCCACTGATACA
NM_016410.5       TCTTATTTGGATGAGGCAGCATCTGCACCTGCAATTCCAGAAGGTGTTCCCACTGATACA
                  ************************************************************

HsCD00587799      AAAAACAAGGATGGAGTTCTGGTGGATGAATTTGGATTGCCACAGATCCCTGCTTCA---
NM_016410.5       AAAAACAAGGATGGAGTTCTGGTGGATGAATTTGGATTGCCACAGATCCCTGCTTCATAG
                  *********************************************************

Pathways

Reactome :

Budding and maturation of HIV virion

Reactome :

Disease

Reactome :

Endosomal Sorting Complex Required For Transport (ESCRT)

Reactome :

HIV Infection

Reactome :

HIV Life Cycle

Reactome :

Late Phase of HIV Life Cycle

Reactome :

Membrane Trafficking

Protein

HPRD :

15408

Clone

Cloning Information :

621333

Original Clone ID :

WISP11-322

Organism

Species Specific ID:

51510

Genome

Chromosome :

Map Location :

9p13.3

Ensembl :

ENSG00000086065

Reagents

Labome :

CHMP5-antibody

CHMP5 (H. sapiens )In pCAG.MCS2.C-Myc (Myc-tagged mammalian expression vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Reference Sequence Alignment

Pathways

Protein

Clone

Organism

Genome

Reagents

CHMP5 (H. sapiens )
In pCAG.MCS2.C-Myc (Myc-tagged mammalian expression vector)