DNASU Plasmid | CHMP5 (Homo sapiens) in pet16B-TEV (Bacterial Expression)

Explanation of Terms

Gene:

Gene Symbol: CHMP5

Gene Name: charged multivesicular body protein 5

Original Clone ID:

WISP10-592

Keyword:

CHMP5 aa 139-191,A191C

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pet16B-TEV

Format:

CLOSED

Source:

University of Utah

Description:

None

Comments:

None

Mutation / Discrepancy:

Yes / No

Publications:

None

Authors:

University of Utah
William Stubblefield
Wesley I. Sundquist

HsCD00587811

Price:

AVAILABLE

No restriction
Special MTA: SPTA

Insert Sequence
Protein Sequence

Insert sequence: 162nts

Open reading frame: 1 to 162

Reference Sequence Alignment

ATGGAAGATGCAAATGAAATCCAAGAAGCACTGAGTCGCAGTTATGGCACCCCAGAACTG
GATGAAGATGATTTAGAAGCAGAGTTGGATGCACTAGGTGATGAGCTTCTGGCTGATGAA
GACAGTTCTTATTTGGATGAGGCAGCATCTGCACCTTGCTAG

Protein sequence predicted based on nucleotide sequence

MEDANEIQEALSRSYGTPELDEDDLEAELDALGDELLADEDSSYLDEAASAPC*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

End read sequencing of cDNA insert

Mutations:

A191C

g157t; c158g; a159c

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pet16B-TEV

Synonyms:

Sequencing Primer:

Forward: T7

Reverse: T7 terminator

Description:

Bacterial expression vector with T7 promoter, N-terminal 10xHis tag and TEV protease cleavage site; ampicillin resistance in bacteria; restriction enzyme cloning

Comments:

Empty vector Sundquist Lab internal database number = WISP10-610

Size (bp):

5714

Parent Vector:

None

Properties:

bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker

Author Name:

Wesley I. Sundquist

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

TTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATG
TCATGATAATAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAAT
GTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTA
TCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAA
GGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTT
GCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGT
AAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGG
ATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTT
CCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCG
TGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGA
ATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGC
ATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACAC
TGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCG
CTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAA
CCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCC
TGCAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTA
CTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTT
GCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGA
TAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGG
GGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGT
CAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTC
ACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTT
AGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATC
CTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCA
CTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTT
TTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCA
GCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGT
AACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGC
CGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTC
GCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTG
TCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGT
CGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACC
TACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCT
TCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAA
CAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTAT
AGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATG
CTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTT
TACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCG
TTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGA
TACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGG
AAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGT
ATTTCACACCGCATATATGGTGCACTCTCAGTACAATCTGCTCTGATGCC
GCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATG
GCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTG
TCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCT
GCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCTG
CGGTAAAGCTCATCAGCGTGGTCGTGAAGCGATTCACAGATGTCTGCCTG
TTCATCCGCGTCCAGCTCGTTGAGTTTCTCCAGAAGCGTTAATGTCTGGC
TTCTGATAAAGCGGGCCATGTTAAGGGCGGTTTTTTCCTGTTTGGTCACT
GATGCCTCCGTGTAAGGGGGATTTCTGTTCATGGGGGTAATGATACCGAT
GAAACGAGAGAGGATGCTCACGATACGGGTTACTGATGATGAACATGCCC
GGTTACTGGAACGTTGTGAGGGTAAACAACTGGCGGTATGGATGCGGCGG
GACCAGAGAAAAATCACTCAGGGTCAATGCCAGCGCTTCGTTAATACAGA
TGTAGGTGTTCCACAGGGTAGCCAGCAGCATCCTGCGATGCAGATCCGGA
ACATAATGGTGCAGGGCGCTGACTTCCGCGTTTCCAGACTTTACGAAACA
CGGAAACCGAAGACCATTCATGTTGTTGCTCAGGTCGCAGACGTTTTGCA
GCAGCAGTCGCTTCACGTTCGCTCGCGTATCGGTGATTCATTCTGCTAAC
CAGTAAGGCAACCCCGCCAGCCTAGCCGGGTCCTCAACGACAGGAGCACG
ATCATGCGCACCCGTGGCCAGGACCCAACGCTGCCCGAGATGCGCCGCGT
GCGGCTGCTGGAGATGGCGGACGCGATGGATATGTTCTGCCAAGGGTTGG
TTTGCGCATTCACAGTTCTCCGCAAGAATTGATTGGCTCCAATTCTTGGA
GTGGTGAATCCGTTAGCGAGGTGCCGCCGGCTTCCATTCAGGTCGAGGTG
GCCCGGCTCCATGCACCGCGACGCAACGCGGGGAGGCAGACAAGGTATAG
GGCGGCGCCTACAATCCATGCCAACCCGTTCCATGTGCTCGCCGAGGCGG
CATAAATCGCCGTGACGATCAGCGGTCCAGTGATCGAAGTTAGGCTGGTA
AGAGCCGCGAGCGATCCTTGAAGCTGTCCCTGATGGTCGTCATCTACCTG
CCTGGACAGCATGGCCTGCAACGCGGGCATCCCGATGCCGCCGGAAGCGA
GAAGAATCATAATGGGGAAGGCCATCCAGCCTCGCGTCGCGAACGCCAGC
AAGACGTAGCCCAGCGCGTCGGCCGCCATGCCGGCGATAATGGCCTGCTT
CTCGCCGAAACGTTTGGTGGCGGGACCAGTGACGAAGGCTTGAGCGAGGG
CGTGCAAGATTCCGAATACCGCAAGCGACAGGCCGATCATCGTCGCGCTC
CAGCGAAAGCGGTCCTCGCCGAAAATGACCCAGAGCGCTGCCGGCACCTG
TCCTACGAGTTGCATGATAAAGAAGACAGTCATAAGTGCGGCGACGATAG
TCATGCCCCGCGCCCACCGGAAGGAGCTGACTGGGTTGAAGGCTCTCAAG
GGCATCGGTCGAGATCCCGGTGCCTAATGAGTGAGCTAACTTACATTAAT
TGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGC
TGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGG
CGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGC
CCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTT
TGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATA
ACATGAGCTGTCTTCGGTATCGTCGTATCCCACTACCGAGATATCCGCAC
CAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCCATC
TGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCCCTCATTCAGCAT
TTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCCCGTT
CCGCTATCGGCTGAATTTGATTGCGAGTGAGATATTTATGCCAGCCAGCC
AGACGCAGACGCGCCGAGACAGAACTTAATGGGCCCGCTAACAGCGCGAT
TTGCTGGTGACCCAATGCGACCAGATGCTCCACGCCCAGTCGCGTACCGT
CTTCATGGGAGAAAATAATACTGTTGATGGGTGTCTGGTCAGAGACATCA
AGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATC
CTGGTCATCCAGCGGATAGTTAATGATCAGCCCACTGACGCGTTGCGCGA
GAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTACC
ATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGC
CGCGACAATTTGCGACGGCGCGTGCAGGGCCAGACTGGAGGTGGCAACGC
CAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGA
ATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGC
AGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGA
CACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACC
ACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGT
TTTGCGCCATTCGATGGTGTCCGGGATCTCGACGCTCTCCCTTATGCGAC
TCCTGCATTAGGAAGCAGCCCAGTAGTAGGTTGAGGCCGTTGAGCACCGC
CGCCGCAAGGAATGGTGCATGCAAGGAGATGGCGCCCAACAGTCCCCCGG
CCACGGGGCCTGCCACCATACCCACGCCGAAACAAGCGCTCATGAGCCCG
AAGTGGCGAGCCCGATCTTCCCCATCGGTGATGTCGGCGATATAGGCGCC
AGCAACCGCACCTGTGGCGCCGGTGATGCCGGCCACGATGCGTCCGGCGT
AGAGGATCGAGATCTCGATCCCGCGAAATTAATACGACTCACTATAGGGG
AATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAACTTT
AAGAAGGAGATATACCATGGGCCATCATCATCATCATCATCATCATCATC
AT AGC GGC CTT GAA GTC CTC TTT CAG GGA CCC CAT ATGCTCGAGGATCCGGCT
GCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCA
ATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTT
TGCTGAAAGGAGGAACTATATCCGGATATCCCGCAAGAGGCCCGGCAGTA
CCGGCATAACCAAGCCTATGCCTACAGCATCCAGGGTGACGGTGCCGAGG
ATGACGATGAGCGCATTGTTAGATTTCATACACGGTGCCTGACTGCGTTA
GCAATTTAACTGTGATAAACTACCGCATTAAAGCTTATCGATGATAAGCT
GTCAAACATGAGAA

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ColE

ColE1 origin

1164

1846

primer

T7 forward

T7 forward sequencing primer TAA TAC GAC TCA CTA TAG G

5231

5246

primer

T7 reverse

T7 reverse sequencing primer

5444

5462

promoter

T7 promoter

5230

5249

protease cleavage site

TEV

TEV protease cleavage site

5359

5382

repressor binding site

lac operator

5249

5271

repressor protein gene

LacI

3761

4840

selectable marker

AmpR

ampicillin resistance

407

1066

tag

His

N-terminal 10XHis tag

5323

5352

trxn termination sequence

T7 term

T7 terminator

5456

5502

Gene

Gene Symbol :

CHMP5

Symbol Nomenclature :

CHMP5

SYNONYM :

C9orf83; HSPC177; PNAS-2; SNF7DC2; Vps60

Designation :

Full Nomenclature :

charged multivesicular body protein 5

GENEID :

51510

Locus Tag :

CGI-34

GenBank Accession :

BC007457

HGNC :

26942

MIM :

610900

Target GenBank:

BC007457

Reference Sequence Alignment

  

HsCD00587811      ------------------------------------------------------------
NM_016410.5       ATGAACCGACTCTTCGGGAAAGCGAAACCCAAGGCTCCGCCGCCCAGCCTGACTGACTGC
                                                                              

HsCD00587811      ------------------------------------------------------------
NM_016410.5       ATTGGCACGGTGGACAGTAGAGCAGAATCCATTGACAAGAAGATTTCTCGATTGGATGCT
                                                                              

HsCD00587811      ------------------------------------------------------------
NM_016410.5       GAGCTAGTGAAGTATAAGGATCAGATCAAGAAGATGAGAGAGGGTCCTGCAAAGAATATG
                                                                              

HsCD00587811      ------------------------------------------------------------
NM_016410.5       GTCAAGCAGAAAGCCTTGCGAGTTTTAAAGCAAAAGAGGATGTATGAGCAGCAGCGGGAC
                                                                              

HsCD00587811      ------------------------------------------------------------
NM_016410.5       AATCTTGCCCAACAGTCATTCAACATGGAACAAGCCAATTATACCATCCAGTCTTTGAAG
                                                                              

HsCD00587811      ------------------------------------------------------------
NM_016410.5       GACACCAAGACCACGGTTGATGCTATGAAACTGGGAGTAAAGGAAATGAAGAAGGCATAC
                                                                              

HsCD00587811      ------------------------------------------------------ATGGAA
NM_016410.5       AAGCAAGTGAAGATCGACCAGATTGAGGATTTACAAGACCAGCTAGAGGATATGATGGAA
                                                                        ******

HsCD00587811      GATGCAAATGAAATCCAAGAAGCACTGAGTCGCAGTTATGGCACCCCAGAACTGGATGAA
NM_016410.5       GATGCAAATGAAATCCAAGAAGCACTGAGTCGCAGTTATGGCACCCCAGAACTGGATGAA
                  ************************************************************

HsCD00587811      GATGATTTAGAAGCAGAGTTGGATGCACTAGGTGATGAGCTTCTGGCTGATGAAGACAGT
NM_016410.5       GATGATTTAGAAGCAGAGTTGGATGCACTAGGTGATGAGCTTCTGGCTGATGAAGACAGT
                  ************************************************************

HsCD00587811      TCTTATTTGGATGAGGCAGCATCTGCACCTTGCTAG------------------------
NM_016410.5       TCTTATTTGGATGAGGCAGCATCTGCACCTGCAATTCCAGAAGGTGTTCCCACTGATACA
                  ******************************  .::                         

HsCD00587811      ------------------------------------------------------------
NM_016410.5       AAAAACAAGGATGGAGTTCTGGTGGATGAATTTGGATTGCCACAGATCCCTGCTTCATAG

Pathways

Reactome :

Budding and maturation of HIV virion

Reactome :

Disease

Reactome :

Endosomal Sorting Complex Required For Transport (ESCRT)

Reactome :

HIV Infection

Reactome :

HIV Life Cycle

Reactome :

Late Phase of HIV Life Cycle

Reactome :

Membrane Trafficking

Protein

HPRD :

15408

Clone

Cloning Information :

621345

Original Clone ID :

WISP10-592

Organism

Species Specific ID:

51510

Genome

Chromosome :

Map Location :

9p13.3

Ensembl :

ENSG00000086065

Reagents

Labome :

CHMP5-antibody